Euprosterna elaeasa virus (isolate Euprosterna elaeasa/-/Zeddam/-) (EvV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Permutotetraviridae; Alphapermutotetravirus; Euprosterna elaeasa virus

Average proteome isoelectric point is 7.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q8QYM6|Q8QYM6_EEVZ Putative replicase OS=Euprosterna elaeasa virus (isolate Euprosterna elaeasa/-/Zeddam/-) OX=1289470 PE=4 SV=1
MM1 pKa = 7.31EE2 pKa = 5.76ASNPVIAPTRR12 pKa = 11.84LSLEE16 pKa = 3.89AMLEE20 pKa = 3.94EE21 pKa = 4.4RR22 pKa = 11.84ASVVRR27 pKa = 11.84QDD29 pKa = 3.75LQSLRR34 pKa = 11.84VRR36 pKa = 11.84LEE38 pKa = 3.72GAPRR42 pKa = 11.84TLAPQAPEE50 pKa = 3.72KK51 pKa = 10.34QGRR54 pKa = 11.84DD55 pKa = 3.33SAQAAAKK62 pKa = 10.44SIVTEE67 pKa = 3.82LRR69 pKa = 11.84DD70 pKa = 3.7AVKK73 pKa = 9.91EE74 pKa = 4.08AQGLEE79 pKa = 4.19HH80 pKa = 7.14KK81 pKa = 10.84SLDD84 pKa = 3.51FLEE87 pKa = 4.39QLGEE91 pKa = 4.16YY92 pKa = 8.19PVCGIIHH99 pKa = 7.28GDD101 pKa = 3.73HH102 pKa = 7.53PIHH105 pKa = 7.47PSGTNNNKK113 pKa = 10.01GKK115 pKa = 10.8VSVQRR120 pKa = 11.84QFTTSMNSVDD130 pKa = 3.9ALQVALRR137 pKa = 11.84YY138 pKa = 9.9EE139 pKa = 4.36DD140 pKa = 3.71VPLVRR145 pKa = 11.84DD146 pKa = 3.42IALKK150 pKa = 7.92TTYY153 pKa = 10.32TDD155 pKa = 3.18GSLAGFVEE163 pKa = 4.22RR164 pKa = 11.84LKK166 pKa = 11.22LQTTRR171 pKa = 11.84PCVQEE176 pKa = 3.93KK177 pKa = 9.91VSRR180 pKa = 11.84RR181 pKa = 11.84LLRR184 pKa = 11.84EE185 pKa = 4.08LFPYY189 pKa = 10.33DD190 pKa = 3.48PQKK193 pKa = 11.04LVDD196 pKa = 3.74VSRR199 pKa = 11.84EE200 pKa = 3.8LSDD203 pKa = 3.97LVLEE207 pKa = 4.68IKK209 pKa = 9.86TNATASAGPPYY220 pKa = 9.72WRR222 pKa = 11.84SKK224 pKa = 10.7RR225 pKa = 11.84DD226 pKa = 3.68ALPDD230 pKa = 3.42MLDD233 pKa = 3.35CVLPLLYY240 pKa = 10.3QHH242 pKa = 6.95IVDD245 pKa = 4.99GDD247 pKa = 3.87LATLKK252 pKa = 10.55NKK254 pKa = 10.18HH255 pKa = 6.52PEE257 pKa = 3.91LFLAEE262 pKa = 4.84CKK264 pKa = 10.73NKK266 pKa = 8.46TDD268 pKa = 3.36RR269 pKa = 11.84YY270 pKa = 8.59EE271 pKa = 3.96VEE273 pKa = 4.08KK274 pKa = 10.87LGEE277 pKa = 3.82KK278 pKa = 8.15TRR280 pKa = 11.84PYY282 pKa = 10.1FSHH285 pKa = 7.38PFHH288 pKa = 6.91LSALVSVLSQSFSKK302 pKa = 10.6ALKK305 pKa = 10.4IMTEE309 pKa = 3.8EE310 pKa = 4.0STSYY314 pKa = 10.44NAYY317 pKa = 9.77GFSWTNGGAEE327 pKa = 4.9DD328 pKa = 3.72LVIWARR334 pKa = 11.84GAGEE338 pKa = 4.11VGKK341 pKa = 9.97EE342 pKa = 3.99PPRR345 pKa = 11.84IACYY349 pKa = 10.76GDD351 pKa = 3.48DD352 pKa = 3.29TDD354 pKa = 4.21IYY356 pKa = 9.93YY357 pKa = 10.03RR358 pKa = 11.84QKK360 pKa = 10.38GRR362 pKa = 11.84LYY364 pKa = 10.4RR365 pKa = 11.84ICPDD369 pKa = 4.09FKK371 pKa = 11.49QMDD374 pKa = 3.92GSVDD378 pKa = 3.23EE379 pKa = 4.47TTIKK383 pKa = 10.83AVVDD387 pKa = 4.19YY388 pKa = 10.49VVHH391 pKa = 6.53AHH393 pKa = 6.02TSQYY397 pKa = 10.99PLAKK401 pKa = 10.35GFWLEE406 pKa = 4.04VGKK409 pKa = 10.5LWVEE413 pKa = 4.29MATSSPFLIDD423 pKa = 2.99GTLVYY428 pKa = 10.24RR429 pKa = 11.84KK430 pKa = 9.15KK431 pKa = 10.78QKK433 pKa = 10.44DD434 pKa = 3.4GLMTGVVGTTLFDD447 pKa = 3.8TVKK450 pKa = 10.74SALAYY455 pKa = 9.81KK456 pKa = 10.42DD457 pKa = 3.17WADD460 pKa = 3.37QLLFGDD466 pKa = 5.3LEE468 pKa = 4.21LLKK471 pKa = 10.27EE472 pKa = 4.08KK473 pKa = 10.67KK474 pKa = 9.9AIKK477 pKa = 9.92FFKK480 pKa = 9.68EE481 pKa = 3.36KK482 pKa = 9.96HH483 pKa = 5.39GLVIKK488 pKa = 10.48EE489 pKa = 4.31GTWNPTAVTEE499 pKa = 4.4DD500 pKa = 3.36PGYY503 pKa = 11.08DD504 pKa = 3.85EE505 pKa = 6.11LWTEE509 pKa = 4.15QKK511 pKa = 10.84FLGLQIKK518 pKa = 7.88VVKK521 pKa = 10.33HH522 pKa = 6.33ADD524 pKa = 3.21TDD526 pKa = 3.75EE527 pKa = 4.43KK528 pKa = 11.38VFVPTLPYY536 pKa = 10.54SDD538 pKa = 4.35WLSMWVTPRR547 pKa = 11.84ARR549 pKa = 11.84LGHH552 pKa = 5.95KK553 pKa = 9.63EE554 pKa = 4.2SYY556 pKa = 9.51TMRR559 pKa = 11.84QRR561 pKa = 11.84TLFDD565 pKa = 3.4RR566 pKa = 11.84ARR568 pKa = 11.84GLLVTGAAFDD578 pKa = 3.61KK579 pKa = 10.69QARR582 pKa = 11.84EE583 pKa = 3.99LMGSVINEE591 pKa = 4.1TPSEE595 pKa = 4.24IVCMKK600 pKa = 9.17VQEE603 pKa = 4.54GGGRR607 pKa = 11.84GAPPSYY613 pKa = 11.27AFLTRR618 pKa = 11.84DD619 pKa = 3.29GNFEE623 pKa = 4.15FPVSDD628 pKa = 4.87GFPDD632 pKa = 4.13LAWTTRR638 pKa = 11.84LYY640 pKa = 11.23SRR642 pKa = 11.84DD643 pKa = 3.29QTGDD647 pKa = 3.26MPRR650 pKa = 11.84VFPDD654 pKa = 3.2AVTLIAAYY662 pKa = 9.96RR663 pKa = 11.84KK664 pKa = 9.69RR665 pKa = 11.84LMDD668 pKa = 2.97TRR670 pKa = 11.84IAVKK674 pKa = 9.94EE675 pKa = 4.22GNVSYY680 pKa = 11.0LMEE683 pKa = 4.13VAVAEE688 pKa = 4.39TGLNMEE694 pKa = 4.69MPAEE698 pKa = 4.13AVVLPPKK705 pKa = 9.95PPSIQKK711 pKa = 9.55PRR713 pKa = 11.84EE714 pKa = 3.44FTYY717 pKa = 11.05AEE719 pKa = 4.12NVSTSTGSLVVKK731 pKa = 10.5KK732 pKa = 10.33VDD734 pKa = 4.02KK735 pKa = 11.32VPDD738 pKa = 3.5SAGTLEE744 pKa = 4.45TIFQKK749 pKa = 10.87FMEE752 pKa = 4.71SVNKK756 pKa = 10.16QIEE759 pKa = 4.26EE760 pKa = 3.93LRR762 pKa = 11.84AGQEE766 pKa = 3.96KK767 pKa = 10.06IEE769 pKa = 4.09RR770 pKa = 11.84TIIMGAALPAEE781 pKa = 5.36AIRR784 pKa = 11.84KK785 pKa = 9.59KK786 pKa = 10.01MGNVDD791 pKa = 3.53LRR793 pKa = 11.84KK794 pKa = 7.68TAEE797 pKa = 3.9KK798 pKa = 10.43AALFTHH804 pKa = 6.71EE805 pKa = 4.53EE806 pKa = 3.98AGDD809 pKa = 4.13LYY811 pKa = 11.39LSTNPVLGHH820 pKa = 6.97PDD822 pKa = 3.05SFEE825 pKa = 4.07SGEE828 pKa = 4.13RR829 pKa = 11.84AKK831 pKa = 10.43EE832 pKa = 3.78VHH834 pKa = 6.2KK835 pKa = 11.19VIGKK839 pKa = 9.71HH840 pKa = 4.51STPEE844 pKa = 3.32RR845 pKa = 11.84KK846 pKa = 9.51ARR848 pKa = 11.84EE849 pKa = 3.97KK850 pKa = 11.09AEE852 pKa = 4.11PVHH855 pKa = 6.46DD856 pKa = 3.37TGEE859 pKa = 4.29FVKK862 pKa = 9.64ITGPAPGGVPTQCEE876 pKa = 4.04PGQTPWYY883 pKa = 8.37SLCRR887 pKa = 11.84SVSKK891 pKa = 10.77AGYY894 pKa = 9.06QVTTRR899 pKa = 11.84TEE901 pKa = 3.75PGLDD905 pKa = 3.07HH906 pKa = 7.07NGRR909 pKa = 11.84PFVRR913 pKa = 11.84AVVLQRR919 pKa = 11.84NMRR922 pKa = 11.84LKK924 pKa = 9.35PQPWQEE930 pKa = 3.91WLVSTGRR937 pKa = 11.84SLARR941 pKa = 11.84AKK943 pKa = 10.35EE944 pKa = 3.89LASDD948 pKa = 3.95YY949 pKa = 11.35ALEE952 pKa = 4.06LVQRR956 pKa = 11.84EE957 pKa = 4.53RR958 pKa = 11.84YY959 pKa = 8.5KK960 pKa = 10.92QPLDD964 pKa = 3.47ATTSWAEE971 pKa = 3.9DD972 pKa = 3.5AEE974 pKa = 4.61LGDD977 pKa = 4.48TIQLLDD983 pKa = 3.53RR984 pKa = 11.84GRR986 pKa = 11.84HH987 pKa = 3.95VFSLIGEE994 pKa = 4.13RR995 pKa = 11.84FEE997 pKa = 4.72RR998 pKa = 11.84VNPGIGTKK1006 pKa = 9.88HH1007 pKa = 5.71QLTDD1011 pKa = 3.62RR1012 pKa = 11.84GDD1014 pKa = 3.64LMVWQPASGTYY1025 pKa = 10.11VVVAPPRR1032 pKa = 11.84KK1033 pKa = 7.14TRR1035 pKa = 11.84SGARR1039 pKa = 11.84SRR1041 pKa = 11.84TLASMVKK1048 pKa = 9.74SVVRR1052 pKa = 11.84ANVDD1056 pKa = 3.26LRR1058 pKa = 11.84VRR1060 pKa = 11.84TAGEE1064 pKa = 3.82PFFRR1068 pKa = 11.84DD1069 pKa = 3.33PVEE1072 pKa = 4.15LSNLKK1077 pKa = 10.48KK1078 pKa = 10.63KK1079 pKa = 10.62INEE1082 pKa = 4.16NKK1084 pKa = 10.16NEE1086 pKa = 4.0AFHH1089 pKa = 6.57EE1090 pKa = 4.47SEE1092 pKa = 4.69DD1093 pKa = 3.77LRR1095 pKa = 11.84SEE1097 pKa = 3.93PEE1099 pKa = 3.86EE1100 pKa = 4.12YY1101 pKa = 10.19QRR1103 pKa = 11.84GPSSSFHH1110 pKa = 6.62EE1111 pKa = 4.56DD1112 pKa = 2.72SPRR1115 pKa = 11.84AGDD1118 pKa = 3.44EE1119 pKa = 4.05HH1120 pKa = 8.77SDD1122 pKa = 3.26ARR1124 pKa = 11.84QHH1126 pKa = 6.76DD1127 pKa = 4.77RR1128 pKa = 11.84VPHH1131 pKa = 4.75VQQLSTKK1138 pKa = 9.5LQGEE1142 pKa = 4.36NRR1144 pKa = 11.84LEE1146 pKa = 4.01ARR1148 pKa = 11.84DD1149 pKa = 3.52ARR1151 pKa = 11.84RR1152 pKa = 11.84DD1153 pKa = 3.49RR1154 pKa = 11.84RR1155 pKa = 11.84EE1156 pKa = 3.59PSLDD1160 pKa = 3.4KK1161 pKa = 11.16EE1162 pKa = 4.49GAEE1165 pKa = 4.5VPQVPSGRR1173 pKa = 11.84PSYY1176 pKa = 10.43RR1177 pKa = 11.84PSLRR1181 pKa = 11.84DD1182 pKa = 3.2PNPRR1186 pKa = 11.84VQQLEE1191 pKa = 4.12EE1192 pKa = 4.06QVRR1195 pKa = 11.84SLQAQITTIAGVRR1208 pKa = 11.84PAPPRR1213 pKa = 11.84GGARR1217 pKa = 11.84KK1218 pKa = 9.68SGNMWRR1224 pKa = 11.84RR1225 pKa = 11.84GGEE1228 pKa = 3.64SRR1230 pKa = 11.84APQRR1234 pKa = 11.84GPGAPGSGGGASQNRR1249 pKa = 11.84KK1250 pKa = 8.47PRR1252 pKa = 11.84GPKK1255 pKa = 9.22SWW1257 pKa = 3.14

Molecular weight:
140.47 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8QYM6|Q8QYM6_EEVZ Putative replicase OS=Euprosterna elaeasa virus (isolate Euprosterna elaeasa/-/Zeddam/-) OX=1289470 PE=4 SV=1
MM1 pKa = 7.93KK2 pKa = 10.15IRR4 pKa = 11.84MRR6 pKa = 11.84LSMNQKK12 pKa = 9.34ILEE15 pKa = 4.33ANLRR19 pKa = 11.84NIKK22 pKa = 9.67EE23 pKa = 4.14GHH25 pKa = 6.64PLAFMRR31 pKa = 11.84TAQGRR36 pKa = 11.84EE37 pKa = 3.61MSIAMLANMIVCPTCNNSLRR57 pKa = 11.84NYY59 pKa = 10.33KK60 pKa = 10.02EE61 pKa = 3.62RR62 pKa = 11.84TDD64 pKa = 3.24WRR66 pKa = 11.84RR67 pKa = 11.84GTLDD71 pKa = 3.75EE72 pKa = 5.11IEE74 pKa = 5.34EE75 pKa = 3.87NLAWIKK81 pKa = 10.93RR82 pKa = 11.84EE83 pKa = 4.07LRR85 pKa = 11.84CLRR88 pKa = 11.84CPPDD92 pKa = 3.5DD93 pKa = 4.17HH94 pKa = 8.95LIDD97 pKa = 3.82QACGIPTPEE106 pKa = 4.2SSNSKK111 pKa = 9.99SKK113 pKa = 10.68SVHH116 pKa = 5.21FKK118 pKa = 10.0HH119 pKa = 7.05RR120 pKa = 11.84SRR122 pKa = 11.84RR123 pKa = 11.84LPEE126 pKa = 4.23SAQLPQGAGRR136 pKa = 11.84GSLVTCGDD144 pKa = 3.51VEE146 pKa = 4.57EE147 pKa = 5.16NPGPPSVARR156 pKa = 11.84GLQEE160 pKa = 4.21VVAGLAKK167 pKa = 10.01TGSPEE172 pKa = 3.43ARR174 pKa = 11.84NPGKK178 pKa = 8.78QARR181 pKa = 11.84RR182 pKa = 11.84RR183 pKa = 11.84RR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84TVKK190 pKa = 9.62GTGNPNQPFAVTAGAALTRR209 pKa = 11.84VPALRR214 pKa = 11.84GTLAMSEE221 pKa = 4.53PISPSSVGWLEE232 pKa = 4.27TNLDD236 pKa = 3.37PCGEE240 pKa = 4.28YY241 pKa = 10.55KK242 pKa = 9.64STLDD246 pKa = 3.49YY247 pKa = 11.6GKK249 pKa = 10.32VPDD252 pKa = 4.3GAIPMSVCGQFRR264 pKa = 11.84EE265 pKa = 4.57TFTIRR270 pKa = 11.84HH271 pKa = 6.18PGANPMVAPLDD282 pKa = 3.65GTMWTLGIIRR292 pKa = 11.84TNLWRR297 pKa = 11.84TPFLLIADD305 pKa = 3.99MRR307 pKa = 11.84NAEE310 pKa = 4.32ISDD313 pKa = 3.76SSLDD317 pKa = 3.47EE318 pKa = 4.11VARR321 pKa = 11.84SFNNYY326 pKa = 9.04VGEE329 pKa = 4.3IEE331 pKa = 5.07LASHH335 pKa = 6.45PNWVEE340 pKa = 3.69TLQEE344 pKa = 3.78GLYY347 pKa = 10.07WSIQRR352 pKa = 11.84WTALIGVPPPSDD364 pKa = 3.21TGVSPYY370 pKa = 9.31ITDD373 pKa = 3.64FRR375 pKa = 11.84MTGSGFTVSSNTPSLINQGMVVVAQFNPNTEE406 pKa = 3.91NKK408 pKa = 9.98EE409 pKa = 4.01IVPHH413 pKa = 5.9SEE415 pKa = 3.83SGEE418 pKa = 4.21TPMGWSRR425 pKa = 11.84VYY427 pKa = 10.93SLSTSQIVTTYY438 pKa = 9.48IQPGVGGAQNTFTVIANRR456 pKa = 11.84GPAVINSSVLPAVTQGYY473 pKa = 9.04RR474 pKa = 11.84FLNGDD479 pKa = 4.05TYY481 pKa = 11.81AVGDD485 pKa = 3.76SLRR488 pKa = 11.84YY489 pKa = 9.75QFTEE493 pKa = 4.11RR494 pKa = 11.84DD495 pKa = 3.37SGGYY499 pKa = 7.1TVYY502 pKa = 10.68LQRR505 pKa = 11.84STDD508 pKa = 2.97GTTFTNIGPPSTTTAAPSGTSTASGVGFILEE539 pKa = 4.18GDD541 pKa = 3.92VLAGRR546 pKa = 11.84VNMLTLPPFTQSDD559 pKa = 3.96LMQQTPKK566 pKa = 9.97TCVFQIKK573 pKa = 10.17EE574 pKa = 3.66GGFYY578 pKa = 10.58VRR580 pKa = 11.84QDD582 pKa = 2.59VWQPVYY588 pKa = 11.58NMTPASRR595 pKa = 11.84YY596 pKa = 8.31APVRR600 pKa = 11.84FVSGAITLDD609 pKa = 3.62SLNSSVGTIRR619 pKa = 11.84DD620 pKa = 3.84TADD623 pKa = 2.99SNYY626 pKa = 10.06GFALCHH632 pKa = 5.18MTSIPLACAPFIKK645 pKa = 10.57AEE647 pKa = 4.02MRR649 pKa = 11.84FEE651 pKa = 4.23AVPGRR656 pKa = 11.84NSPWGLFATSTPPKK670 pKa = 10.54DD671 pKa = 3.62EE672 pKa = 4.02VALTISRR679 pKa = 11.84TVMDD683 pKa = 5.11LEE685 pKa = 4.45PFAMPLAYY693 pKa = 10.46NGWGLMFSKK702 pKa = 10.38IINIVRR708 pKa = 11.84KK709 pKa = 8.82VPKK712 pKa = 10.14VLRR715 pKa = 11.84TASNVADD722 pKa = 3.72VVANCVEE729 pKa = 4.24DD730 pKa = 4.0AQAGATRR737 pKa = 11.84VYY739 pKa = 10.07EE740 pKa = 4.26SVRR743 pKa = 11.84GRR745 pKa = 11.84RR746 pKa = 11.84GAMRR750 pKa = 11.84DD751 pKa = 3.18MTLQQQ756 pKa = 3.28

Molecular weight:
82.7 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2013

756

1257

1006.5

111.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.799 ± 0.067

1.043 ± 0.287

5.017 ± 0.553

6.657 ± 0.928

3.13 ± 0.093

7.601 ± 0.316

1.689 ± 0.332

3.676 ± 0.641

5.067 ± 1.206

8.197 ± 0.555

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.46

3.477 ± 0.885

6.855 ± 0.221

4.074 ± 0.195

7.65 ± 0.128

7.054 ± 0.465

6.955 ± 0.726

7.501 ± 0.119

1.49 ± 0.051

2.633 ± 0.063

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski