Firmicutes bacterium CAG:124

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2364 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5IBY7|R5IBY7_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:124 OX=1263002 GN=BN480_00939 PE=4 SV=1
MM1 pKa = 7.61TKK3 pKa = 10.44RR4 pKa = 11.84LLCVCLALVLLFGVLAGCSKK24 pKa = 11.22NEE26 pKa = 3.89AQTADD31 pKa = 3.67DD32 pKa = 3.96TKK34 pKa = 10.57KK35 pKa = 9.13TEE37 pKa = 4.38QSDD40 pKa = 4.31TEE42 pKa = 4.85DD43 pKa = 3.24KK44 pKa = 11.02TSTQFAYY51 pKa = 9.62QAQYY55 pKa = 11.05FDD57 pKa = 4.81LPEE60 pKa = 5.22DD61 pKa = 3.72IQWIGTSCVTGDD73 pKa = 3.63TLYY76 pKa = 9.23FTASVPDD83 pKa = 4.07GGKK86 pKa = 8.21EE87 pKa = 3.98TYY89 pKa = 9.58TDD91 pKa = 3.56EE92 pKa = 4.4NGEE95 pKa = 4.22EE96 pKa = 3.98ISYY99 pKa = 8.41DD100 pKa = 3.47TYY102 pKa = 11.52SEE104 pKa = 4.43VIFRR108 pKa = 11.84FDD110 pKa = 4.38LDD112 pKa = 3.37TGEE115 pKa = 5.42CVQLDD120 pKa = 4.0NYY122 pKa = 9.58VAEE125 pKa = 4.48PVVEE129 pKa = 4.21NPDD132 pKa = 3.52MPDD135 pKa = 3.35GVTMNPDD142 pKa = 2.67GSMQTFNSSTSIQTMAAGADD162 pKa = 3.73GTLWLYY168 pKa = 10.7RR169 pKa = 11.84QTSRR173 pKa = 11.84YY174 pKa = 9.98ADD176 pKa = 4.32DD177 pKa = 3.77GTEE180 pKa = 4.04GDD182 pKa = 4.82SISEE186 pKa = 4.6LIQLDD191 pKa = 3.35AHH193 pKa = 5.81GTLLRR198 pKa = 11.84TITPVSDD205 pKa = 3.71EE206 pKa = 4.32EE207 pKa = 4.5TDD209 pKa = 4.08DD210 pKa = 3.77TDD212 pKa = 2.76SWRR215 pKa = 11.84YY216 pKa = 7.86TYY218 pKa = 10.31IDD220 pKa = 4.96SILSDD225 pKa = 3.7DD226 pKa = 3.48KK227 pKa = 11.63GYY229 pKa = 11.32VYY231 pKa = 10.18TYY233 pKa = 10.4DD234 pKa = 3.62YY235 pKa = 10.54QTVNVYY241 pKa = 10.54GPDD244 pKa = 3.39GSFVFSKK251 pKa = 10.86SGDD254 pKa = 3.61EE255 pKa = 4.64LNGQICQLSASEE267 pKa = 4.33VGMTTSSADD276 pKa = 2.85GKK278 pKa = 10.04MVFKK282 pKa = 10.91QLDD285 pKa = 4.06PEE287 pKa = 4.36TKK289 pKa = 9.93DD290 pKa = 3.02WGKK293 pKa = 8.21EE294 pKa = 4.08TPVSSRR300 pKa = 11.84AWNILPGNDD309 pKa = 2.63VYY311 pKa = 11.54AYY313 pKa = 10.76FFMDD317 pKa = 3.08NGNIFGEE324 pKa = 4.08RR325 pKa = 11.84RR326 pKa = 11.84DD327 pKa = 3.59NGEE330 pKa = 3.8VEE332 pKa = 5.18KK333 pKa = 11.29VVDD336 pKa = 4.11WVACDD341 pKa = 3.35VDD343 pKa = 4.64SNNINSDD350 pKa = 3.16QFGFLSDD357 pKa = 3.5GRR359 pKa = 11.84IVAVTYY365 pKa = 10.09DD366 pKa = 3.74YY367 pKa = 11.82SDD369 pKa = 4.9DD370 pKa = 4.25GPSKK374 pKa = 10.5QQILVLNRR382 pKa = 11.84VDD384 pKa = 3.55AASVTAKK391 pKa = 10.16TEE393 pKa = 3.87LTLACLYY400 pKa = 10.79LDD402 pKa = 3.67YY403 pKa = 11.02NLRR406 pKa = 11.84SQIVKK411 pKa = 9.97FNKK414 pKa = 9.9SNPDD418 pKa = 3.01YY419 pKa = 10.81RR420 pKa = 11.84IVVKK424 pKa = 10.46DD425 pKa = 3.51YY426 pKa = 11.6SEE428 pKa = 4.24YY429 pKa = 10.76ATDD432 pKa = 3.95DD433 pKa = 4.21DD434 pKa = 5.08YY435 pKa = 12.16NAGLTKK441 pKa = 10.79LNTEE445 pKa = 4.64IISGNVPDD453 pKa = 4.15ILVNGTEE460 pKa = 4.29LPIGQYY466 pKa = 9.19AAKK469 pKa = 10.46GLLEE473 pKa = 4.91DD474 pKa = 4.02LWPYY478 pKa = 11.2LDD480 pKa = 5.38ADD482 pKa = 3.91PEE484 pKa = 4.2YY485 pKa = 11.3SRR487 pKa = 11.84DD488 pKa = 3.48KK489 pKa = 11.51LMSQPLNAAQTDD501 pKa = 3.83GKK503 pKa = 10.69LYY505 pKa = 10.44RR506 pKa = 11.84LPIDD510 pKa = 4.13FGVTTAVGLGKK521 pKa = 10.48VVGEE525 pKa = 4.1YY526 pKa = 7.33TTWTLADD533 pKa = 3.71VNDD536 pKa = 4.71ALSKK540 pKa = 10.41LPEE543 pKa = 4.39GATVFNKK550 pKa = 10.43YY551 pKa = 7.23YY552 pKa = 9.27TQAEE556 pKa = 4.17MLQYY560 pKa = 10.05CIAMNAEE567 pKa = 5.11SFMNWQDD574 pKa = 3.55GTCSFDD580 pKa = 3.31TDD582 pKa = 3.31EE583 pKa = 5.29FRR585 pKa = 11.84ALLEE589 pKa = 4.09FVKK592 pKa = 10.2PFPAEE597 pKa = 3.9YY598 pKa = 9.96DD599 pKa = 3.48WQSDD603 pKa = 3.5SDD605 pKa = 4.74DD606 pKa = 4.04YY607 pKa = 11.84EE608 pKa = 5.05SDD610 pKa = 3.5YY611 pKa = 11.64TRR613 pKa = 11.84LKK615 pKa = 10.73NGKK618 pKa = 8.22QLLYY622 pKa = 8.15PTSLSGFSDD631 pKa = 4.29LYY633 pKa = 10.01YY634 pKa = 10.94TFAALNNDD642 pKa = 2.27IRR644 pKa = 11.84FIGFPRR650 pKa = 11.84EE651 pKa = 4.24DD652 pKa = 3.63GSSGNAFNASCTLSISTTCKK672 pKa = 10.34DD673 pKa = 4.24KK674 pKa = 11.32SGAWAFIRR682 pKa = 11.84STLSDD687 pKa = 4.54DD688 pKa = 3.78YY689 pKa = 11.66QEE691 pKa = 5.63SIWNYY696 pKa = 9.42PIVKK700 pKa = 10.02SVFEE704 pKa = 4.45AKK706 pKa = 10.2AQEE709 pKa = 4.19AMTQEE714 pKa = 4.5YY715 pKa = 7.69EE716 pKa = 3.76TDD718 pKa = 3.33ADD720 pKa = 4.08GNQILDD726 pKa = 4.09DD727 pKa = 5.28DD728 pKa = 5.25GNPIPISSGGMSYY741 pKa = 11.28GNEE744 pKa = 3.88PMIEE748 pKa = 4.31LYY750 pKa = 10.74AVTQEE755 pKa = 4.22QYY757 pKa = 11.37DD758 pKa = 3.77AVLALIDD765 pKa = 3.79STTTFVDD772 pKa = 3.28YY773 pKa = 10.76DD774 pKa = 3.85QNVLDD779 pKa = 5.24IISDD783 pKa = 3.5EE784 pKa = 4.1AAGYY788 pKa = 8.73FAGSKK793 pKa = 8.16TVEE796 pKa = 4.25EE797 pKa = 4.16ASKK800 pKa = 10.69LIQSRR805 pKa = 11.84VSLYY809 pKa = 10.27IQEE812 pKa = 4.22QKK814 pKa = 11.15

Molecular weight: 90.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5IIM2|R5IIM2_9FIRM Filamentation protein Fic1 OS=Firmicutes bacterium CAG:124 OX=1263002 GN=BN480_00069 PE=4 SV=1
MM1 pKa = 7.09ATVRR5 pKa = 11.84TYY7 pKa = 10.02QPRR10 pKa = 11.84KK11 pKa = 7.15RR12 pKa = 11.84HH13 pKa = 5.32RR14 pKa = 11.84SKK16 pKa = 10.5VHH18 pKa = 5.66GFRR21 pKa = 11.84KK22 pKa = 10.08RR23 pKa = 11.84MATANGRR30 pKa = 11.84KK31 pKa = 8.93VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.49GRR41 pKa = 11.84AKK43 pKa = 10.69LSAA46 pKa = 3.92

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2364

0

2364

714807

29

2678

302.4

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.31 ± 0.057

1.855 ± 0.027

5.61 ± 0.045

6.547 ± 0.047

3.784 ± 0.035

7.59 ± 0.047

1.801 ± 0.023

5.643 ± 0.049

4.79 ± 0.049

9.96 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.026

3.252 ± 0.031

4.122 ± 0.035

3.643 ± 0.034

5.544 ± 0.054

5.54 ± 0.043

5.808 ± 0.056

6.848 ± 0.036

1.016 ± 0.021

3.61 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski