Amino acid dipepetide frequency for Firmicutes bacterium CAG:124

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.91AlaAla: 13.91 ± 0.223	1.851AlaCys: 1.851 ± 0.056	5.856AlaAsp: 5.856 ± 0.1	7.511AlaGlu: 7.511 ± 0.119	3.615AlaPhe: 3.615 ± 0.075	8.181AlaGly: 8.181 ± 0.108	1.675AlaHis: 1.675 ± 0.054	5.407AlaIle: 5.407 ± 0.09	4.814AlaLys: 4.814 ± 0.081	10.817AlaLeu: 10.817 ± 0.165	2.892AlaMet: 2.892 ± 0.062	2.606AlaAsn: 2.606 ± 0.073	3.67AlaPro: 3.67 ± 0.087	4.316AlaGln: 4.316 ± 0.08	5.013AlaArg: 5.013 ± 0.092	5.164AlaSer: 5.164 ± 0.106	3.437AlaThr: 3.437 ± 0.09	8.177AlaVal: 8.177 ± 0.128	0.911AlaTrp: 0.911 ± 0.036	3.278AlaTyr: 3.278 ± 0.077	0.0AlaXaa: 0.0 ± 0.0
Cys	2.096CysAla: 2.096 ± 0.06	0.388CysCys: 0.388 ± 0.024	1.014CysAsp: 1.014 ± 0.039	1.093CysGlu: 1.093 ± 0.042	0.704CysPhe: 0.704 ± 0.034	2.029CysGly: 2.029 ± 0.063	0.318CysHis: 0.318 ± 0.02	1.046CysIle: 1.046 ± 0.036	0.755CysLys: 0.755 ± 0.034	1.384CysLeu: 1.384 ± 0.047	0.508CysMet: 0.508 ± 0.028	0.516CysAsn: 0.516 ± 0.026	0.883CysPro: 0.883 ± 0.036	0.46CysGln: 0.46 ± 0.026	1.273CysArg: 1.273 ± 0.044	0.936CysSer: 0.936 ± 0.037	1.03CysThr: 1.03 ± 0.041	1.356CysVal: 1.356 ± 0.048	0.183CysTrp: 0.183 ± 0.016	0.578CysTyr: 0.578 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	6.325AspAla: 6.325 ± 0.096	1.102AspCys: 1.102 ± 0.045	2.864AspAsp: 2.864 ± 0.089	4.351AspGlu: 4.351 ± 0.077	2.374AspPhe: 2.374 ± 0.052	5.371AspGly: 5.371 ± 0.113	0.895AspHis: 0.895 ± 0.04	3.155AspIle: 3.155 ± 0.067	2.465AspLys: 2.465 ± 0.066	4.842AspLeu: 4.842 ± 0.089	1.5AspMet: 1.5 ± 0.043	1.514AspAsn: 1.514 ± 0.05	2.311AspPro: 2.311 ± 0.062	1.217AspGln: 1.217 ± 0.044	2.939AspArg: 2.939 ± 0.076	2.72AspSer: 2.72 ± 0.069	3.316AspThr: 3.316 ± 0.068	3.821AspVal: 3.821 ± 0.086	0.699AspTrp: 0.699 ± 0.032	2.321AspTyr: 2.321 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	7.014GluAla: 7.014 ± 0.106	0.697GluCys: 0.697 ± 0.026	3.511GluAsp: 3.511 ± 0.087	4.418GluGlu: 4.418 ± 0.094	2.094GluPhe: 2.094 ± 0.053	3.598GluGly: 3.598 ± 0.075	1.291GluHis: 1.291 ± 0.047	4.14GluIle: 4.14 ± 0.079	4.348GluLys: 4.348 ± 0.086	7.083GluLeu: 7.083 ± 0.131	1.749GluMet: 1.749 ± 0.058	3.058GluAsn: 3.058 ± 0.07	2.658GluPro: 2.658 ± 0.071	3.485GluGln: 3.485 ± 0.088	3.735GluArg: 3.735 ± 0.077	2.832GluSer: 2.832 ± 0.069	3.807GluThr: 3.807 ± 0.078	3.248GluVal: 3.248 ± 0.064	0.523GluTrp: 0.523 ± 0.032	2.181GluTyr: 2.181 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	3.762PheAla: 3.762 ± 0.084	0.935PheCys: 0.935 ± 0.035	2.57PheAsp: 2.57 ± 0.057	2.091PheGlu: 2.091 ± 0.064	1.599PhePhe: 1.599 ± 0.058	3.206PheGly: 3.206 ± 0.067	0.751PheHis: 0.751 ± 0.032	1.817PheIle: 1.817 ± 0.047	1.167PheLys: 1.167 ± 0.046	3.67PheLeu: 3.67 ± 0.078	0.87PheMet: 0.87 ± 0.037	1.056PheAsn: 1.056 ± 0.041	1.484PhePro: 1.484 ± 0.045	1.038PheGln: 1.038 ± 0.04	2.072PheArg: 2.072 ± 0.062	2.867PheSer: 2.867 ± 0.067	2.342PheThr: 2.342 ± 0.064	2.608PheVal: 2.608 ± 0.071	0.4PheTrp: 0.4 ± 0.024	1.532PheTyr: 1.532 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	6.947GlyAla: 6.947 ± 0.128	1.665GlyCys: 1.665 ± 0.055	3.702GlyAsp: 3.702 ± 0.076	4.712GlyGlu: 4.712 ± 0.09	3.173GlyPhe: 3.173 ± 0.071	5.944GlyGly: 5.944 ± 0.109	1.356GlyHis: 1.356 ± 0.052	4.625GlyIle: 4.625 ± 0.088	4.545GlyLys: 4.545 ± 0.089	6.763GlyLeu: 6.763 ± 0.125	2.423GlyMet: 2.423 ± 0.06	2.282GlyAsn: 2.282 ± 0.063	1.819GlyPro: 1.819 ± 0.048	2.521GlyGln: 2.521 ± 0.074	4.049GlyArg: 4.049 ± 0.075	4.612GlySer: 4.612 ± 0.099	4.892GlyThr: 4.892 ± 0.126	5.767GlyVal: 5.767 ± 0.102	0.979GlyTrp: 0.979 ± 0.04	3.125GlyTyr: 3.125 ± 0.084	0.003GlyXaa: 0.003 ± 0.002
His	1.684HisAla: 1.684 ± 0.05	0.372HisCys: 0.372 ± 0.026	1.067HisAsp: 1.067 ± 0.043	1.07HisGlu: 1.07 ± 0.048	0.757HisPhe: 0.757 ± 0.034	1.554HisGly: 1.554 ± 0.047	0.478HisHis: 0.478 ± 0.039	1.249HisIle: 1.249 ± 0.043	0.706HisLys: 0.706 ± 0.032	1.556HisLeu: 1.556 ± 0.057	0.448HisMet: 0.448 ± 0.023	0.592HisAsn: 0.592 ± 0.032	0.992HisPro: 0.992 ± 0.037	0.512HisGln: 0.512 ± 0.025	0.881HisArg: 0.881 ± 0.037	0.872HisSer: 0.872 ± 0.036	1.16HisThr: 1.16 ± 0.047	1.203HisVal: 1.203 ± 0.039	0.224HisTrp: 0.224 ± 0.017	0.635HisTyr: 0.635 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.748IleAla: 5.748 ± 0.089	1.174IleCys: 1.174 ± 0.041	3.356IleAsp: 3.356 ± 0.074	3.412IleGlu: 3.412 ± 0.07	2.026IlePhe: 2.026 ± 0.064	4.425IleGly: 4.425 ± 0.089	1.013IleHis: 1.013 ± 0.04	2.966IleIle: 2.966 ± 0.075	2.152IleLys: 2.152 ± 0.059	5.775IleLeu: 5.775 ± 0.113	1.336IleMet: 1.336 ± 0.04	1.785IleAsn: 1.785 ± 0.053	2.706IlePro: 2.706 ± 0.058	1.609IleGln: 1.609 ± 0.052	3.366IleArg: 3.366 ± 0.07	3.632IleSer: 3.632 ± 0.074	3.495IleThr: 3.495 ± 0.075	4.13IleVal: 4.13 ± 0.085	0.501IleTrp: 0.501 ± 0.028	1.821IleTyr: 1.821 ± 0.057	0.001IleXaa: 0.001 ± 0.001
Lys	4.496LysAla: 4.496 ± 0.097	0.588LysCys: 0.588 ± 0.03	2.507LysAsp: 2.507 ± 0.066	2.922LysGlu: 2.922 ± 0.072	1.284LysPhe: 1.284 ± 0.049	2.886LysGly: 2.886 ± 0.064	0.726LysHis: 0.726 ± 0.034	2.806LysIle: 2.806 ± 0.067	3.581LysLys: 3.581 ± 0.079	4.903LysLeu: 4.903 ± 0.089	1.337LysMet: 1.337 ± 0.046	2.185LysAsn: 2.185 ± 0.059	2.142LysPro: 2.142 ± 0.054	1.987LysGln: 1.987 ± 0.056	2.783LysArg: 2.783 ± 0.066	2.41LysSer: 2.41 ± 0.058	3.549LysThr: 3.549 ± 0.081	2.623LysVal: 2.623 ± 0.069	0.46LysTrp: 0.46 ± 0.028	1.719LysTyr: 1.719 ± 0.053	0.0LysXaa: 0.0 ± 0.0
Leu	10.064LeuAla: 10.064 ± 0.163	2.269LeuCys: 2.269 ± 0.071	5.753LeuAsp: 5.753 ± 0.093	5.716LeuGlu: 5.716 ± 0.1	3.714LeuPhe: 3.714 ± 0.091	6.792LeuGly: 6.792 ± 0.116	2.11LeuHis: 2.11 ± 0.064	4.815LeuIle: 4.815 ± 0.096	4.207LeuLys: 4.207 ± 0.09	10.779LeuLeu: 10.779 ± 0.209	2.528LeuMet: 2.528 ± 0.067	2.998LeuAsn: 2.998 ± 0.064	5.089LeuPro: 5.089 ± 0.101	3.296LeuGln: 3.296 ± 0.068	6.329LeuArg: 6.329 ± 0.118	6.556LeuSer: 6.556 ± 0.099	6.42LeuThr: 6.42 ± 0.106	5.958LeuVal: 5.958 ± 0.12	0.883LeuTrp: 0.883 ± 0.037	3.32LeuTyr: 3.32 ± 0.075	0.003LeuXaa: 0.003 ± 0.002
Met	2.588MetAla: 2.588 ± 0.06	0.347MetCys: 0.347 ± 0.02	1.54MetAsp: 1.54 ± 0.044	1.9MetGlu: 1.9 ± 0.061	0.82MetPhe: 0.82 ± 0.037	1.747MetGly: 1.747 ± 0.055	0.459MetHis: 0.459 ± 0.024	1.543MetIle: 1.543 ± 0.053	1.771MetLys: 1.771 ± 0.047	2.85MetLeu: 2.85 ± 0.071	0.765MetMet: 0.765 ± 0.032	1.179MetAsn: 1.179 ± 0.04	1.132MetPro: 1.132 ± 0.039	1.442MetGln: 1.442 ± 0.043	1.533MetArg: 1.533 ± 0.049	1.36MetSer: 1.36 ± 0.044	1.861MetThr: 1.861 ± 0.048	1.507MetVal: 1.507 ± 0.05	0.182MetTrp: 0.182 ± 0.018	0.739MetTyr: 0.739 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.415AsnAla: 3.415 ± 0.077	0.527AsnCys: 0.527 ± 0.026	1.605AsnAsp: 1.605 ± 0.053	1.949AsnGlu: 1.949 ± 0.054	1.196AsnPhe: 1.196 ± 0.046	3.086AsnGly: 3.086 ± 0.079	0.561AsnHis: 0.561 ± 0.029	2.09AsnIle: 2.09 ± 0.051	1.269AsnLys: 1.269 ± 0.042	3.018AsnLeu: 3.018 ± 0.071	0.907AsnMet: 0.907 ± 0.038	1.072AsnAsn: 1.072 ± 0.05	1.683AsnPro: 1.683 ± 0.06	0.981AsnGln: 0.981 ± 0.037	1.809AsnArg: 1.809 ± 0.048	1.528AsnSer: 1.528 ± 0.051	1.854AsnThr: 1.854 ± 0.056	2.292AsnVal: 2.292 ± 0.064	0.379AsnTrp: 0.379 ± 0.028	1.3AsnTyr: 1.3 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	4.537ProAla: 4.537 ± 0.086	0.651ProCys: 0.651 ± 0.027	3.159ProAsp: 3.159 ± 0.063	4.225ProGlu: 4.225 ± 0.087	1.519ProPhe: 1.519 ± 0.051	3.09ProGly: 3.09 ± 0.065	0.649ProHis: 0.649 ± 0.03	1.975ProIle: 1.975 ± 0.055	1.774ProLys: 1.774 ± 0.056	3.38ProLeu: 3.38 ± 0.093	1.058ProMet: 1.058 ± 0.038	1.26ProAsn: 1.26 ± 0.047	1.286ProPro: 1.286 ± 0.06	1.637ProGln: 1.637 ± 0.054	1.553ProArg: 1.553 ± 0.048	1.956ProSer: 1.956 ± 0.057	1.919ProThr: 1.919 ± 0.059	3.639ProVal: 3.639 ± 0.083	0.39ProTrp: 0.39 ± 0.024	1.558ProTyr: 1.558 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	3.826GlnAla: 3.826 ± 0.086	0.459GlnCys: 0.459 ± 0.025	1.679GlnAsp: 1.679 ± 0.056	2.471GlnGlu: 2.471 ± 0.065	1.206GlnPhe: 1.206 ± 0.044	1.823GlnGly: 1.823 ± 0.054	0.635GlnHis: 0.635 ± 0.031	2.408GlnIle: 2.408 ± 0.063	2.126GlnLys: 2.126 ± 0.056	3.693GlnLeu: 3.693 ± 0.085	1.147GlnMet: 1.147 ± 0.048	1.504GlnAsn: 1.504 ± 0.046	1.689GlnPro: 1.689 ± 0.058	1.61GlnGln: 1.61 ± 0.057	1.977GlnArg: 1.977 ± 0.063	1.945GlnSer: 1.945 ± 0.057	2.608GlnThr: 2.608 ± 0.058	1.861GlnVal: 1.861 ± 0.052	0.355GlnTrp: 0.355 ± 0.024	1.405GlnTyr: 1.405 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	5.239ArgAla: 5.239 ± 0.102	0.965ArgCys: 0.965 ± 0.042	2.643ArgAsp: 2.643 ± 0.064	4.099ArgGlu: 4.099 ± 0.091	2.233ArgPhe: 2.233 ± 0.064	3.436ArgGly: 3.436 ± 0.064	0.986ArgHis: 0.986 ± 0.041	3.37ArgIle: 3.37 ± 0.068	2.741ArgLys: 2.741 ± 0.061	5.727ArgLeu: 5.727 ± 0.111	1.778ArgMet: 1.778 ± 0.056	1.631ArgAsn: 1.631 ± 0.053	2.026ArgPro: 2.026 ± 0.065	2.499ArgGln: 2.499 ± 0.063	3.846ArgArg: 3.846 ± 0.095	2.823ArgSer: 2.823 ± 0.065	3.116ArgThr: 3.116 ± 0.067	3.587ArgVal: 3.587 ± 0.076	0.569ArgTrp: 0.569 ± 0.031	2.121ArgTyr: 2.121 ± 0.06	0.001ArgXaa: 0.001 ± 0.002
Ser	5.732SerAla: 5.732 ± 0.11	1.102SerCys: 1.102 ± 0.042	3.137SerAsp: 3.137 ± 0.075	3.372SerGlu: 3.372 ± 0.072	2.486SerPhe: 2.486 ± 0.068	5.539SerGly: 5.539 ± 0.106	0.933SerHis: 0.933 ± 0.034	3.071SerIle: 3.071 ± 0.067	2.084SerLys: 2.084 ± 0.062	4.969SerLeu: 4.969 ± 0.097	1.553SerMet: 1.553 ± 0.047	1.637SerAsn: 1.637 ± 0.053	1.943SerPro: 1.943 ± 0.057	1.628SerGln: 1.628 ± 0.055	3.138SerArg: 3.138 ± 0.068	3.153SerSer: 3.153 ± 0.087	2.83SerThr: 2.83 ± 0.072	4.366SerVal: 4.366 ± 0.089	0.638SerTrp: 0.638 ± 0.036	2.083SerTyr: 2.083 ± 0.067	0.001SerXaa: 0.001 ± 0.001
Thr	6.34ThrAla: 6.34 ± 0.122	0.879ThrCys: 0.879 ± 0.038	3.384ThrAsp: 3.384 ± 0.071	3.457ThrGlu: 3.457 ± 0.079	2.181ThrPhe: 2.181 ± 0.067	5.22ThrGly: 5.22 ± 0.114	1.004ThrHis: 1.004 ± 0.042	3.503ThrIle: 3.503 ± 0.076	2.359ThrLys: 2.359 ± 0.06	6.03ThrLeu: 6.03 ± 0.112	1.409ThrMet: 1.409 ± 0.046	1.752ThrAsn: 1.752 ± 0.054	2.876ThrPro: 2.876 ± 0.07	1.949ThrGln: 1.949 ± 0.057	2.604ThrArg: 2.604 ± 0.073	2.679ThrSer: 2.679 ± 0.07	2.885ThrThr: 2.885 ± 0.086	5.113ThrVal: 5.113 ± 0.131	0.56ThrTrp: 0.56 ± 0.032	1.898ThrTyr: 1.898 ± 0.079	0.0ThrXaa: 0.0 ± 0.0
Val	5.113ValAla: 5.113 ± 0.096	1.683ValCys: 1.683 ± 0.044	3.629ValAsp: 3.629 ± 0.08	3.808ValGlu: 3.808 ± 0.087	2.918ValPhe: 2.918 ± 0.076	4.393ValGly: 4.393 ± 0.093	1.235ValHis: 1.235 ± 0.047	4.004ValIle: 4.004 ± 0.097	3.194ValLys: 3.194 ± 0.069	7.672ValLeu: 7.672 ± 0.112	1.977ValMet: 1.977 ± 0.05	2.236ValAsn: 2.236 ± 0.06	3.004ValPro: 3.004 ± 0.064	2.472ValGln: 2.472 ± 0.065	3.886ValArg: 3.886 ± 0.082	4.824ValSer: 4.824 ± 0.087	4.633ValThr: 4.633 ± 0.139	4.496ValVal: 4.496 ± 0.098	0.782ValTrp: 0.782 ± 0.039	2.524ValTyr: 2.524 ± 0.065	0.001ValXaa: 0.001 ± 0.001
Trp	0.814TrpAla: 0.814 ± 0.038	0.196TrpCys: 0.196 ± 0.017	0.614TrpAsp: 0.614 ± 0.031	0.541TrpGlu: 0.541 ± 0.03	0.438TrpPhe: 0.438 ± 0.025	0.72TrpGly: 0.72 ± 0.035	0.214TrpHis: 0.214 ± 0.015	0.52TrpIle: 0.52 ± 0.029	0.539TrpLys: 0.539 ± 0.027	1.118TrpLeu: 1.118 ± 0.05	0.299TrpMet: 0.299 ± 0.023	0.42TrpAsn: 0.42 ± 0.023	0.312TrpPro: 0.312 ± 0.021	0.523TrpGln: 0.523 ± 0.026	0.576TrpArg: 0.576 ± 0.029	0.609TrpSer: 0.609 ± 0.029	0.569TrpThr: 0.569 ± 0.033	0.568TrpVal: 0.568 ± 0.028	0.134TrpTrp: 0.134 ± 0.013	0.435TrpTyr: 0.435 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.446TyrAla: 3.446 ± 0.076	0.699TyrCys: 0.699 ± 0.034	2.511TyrAsp: 2.511 ± 0.073	2.352TyrGlu: 2.352 ± 0.061	1.498TyrPhe: 1.498 ± 0.045	2.861TyrGly: 2.861 ± 0.071	0.723TyrHis: 0.723 ± 0.032	1.917TyrIle: 1.917 ± 0.05	1.3TyrLys: 1.3 ± 0.046	3.569TyrLeu: 3.569 ± 0.081	0.781TyrMet: 0.781 ± 0.034	1.235TyrAsn: 1.235 ± 0.047	1.512TyrPro: 1.512 ± 0.039	1.242TyrGln: 1.242 ± 0.042	2.075TyrArg: 2.075 ± 0.055	1.921TyrSer: 1.921 ± 0.062	2.359TyrThr: 2.359 ± 0.087	2.166TyrVal: 2.166 ± 0.065	0.407TyrTrp: 0.407 ± 0.022	1.528TyrTyr: 1.528 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.003XaaGly: 0.003 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.013XaaXaa: 0.013 ± 0.005

Statistics based on 2364 proteins (714807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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