Thioclava sp. SK-1
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3819 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C2HNN6|A0A1C2HNN6_9RHOB Peptidase_M48 domain-containing protein OS=Thioclava sp. SK-1 OX=1889770 GN=BFP70_09865 PE=3 SV=1
MM1 pKa = 7.74 AILWGLGGPAAHH13 pKa = 7.12 AGDD16 pKa = 4.51 FQIDD20 pKa = 3.34 WEE22 pKa = 4.37 AFDD25 pKa = 3.98 WPAGVTGPLVRR36 pKa = 11.84 TLRR39 pKa = 11.84 DD40 pKa = 3.36 QNGFEE45 pKa = 4.08 VDD47 pKa = 3.45 VTVTHH52 pKa = 6.95 TGPFAGYY59 pKa = 8.93 PDD61 pKa = 4.31 GAGGTIPTPDD71 pKa = 3.92 DD72 pKa = 3.25 VTIFGGSTEE81 pKa = 4.12 SLILVVDD88 pKa = 4.58 APQNQGAIGDD98 pKa = 3.96 SRR100 pKa = 11.84 TISTVSASSGGIAILVDD117 pKa = 4.05 DD118 pKa = 4.51 LTIDD122 pKa = 3.61 VFDD125 pKa = 5.55 IDD127 pKa = 4.01 STDD130 pKa = 3.3 NNAVSDD136 pKa = 3.84 RR137 pKa = 11.84 CDD139 pKa = 3.3 FLTVFGDD146 pKa = 3.47 NGNPTLSTLSLTPSVVVGPGFGSGLTGPITANQAQCIYY184 pKa = 10.77 FEE186 pKa = 5.12 GPTGSPTSPNDD197 pKa = 3.17 DD198 pKa = 3.76 TGSVRR203 pKa = 11.84 ATFPDD208 pKa = 3.39 ATSSVTFWYY217 pKa = 10.15 DD218 pKa = 3.06 EE219 pKa = 4.41 SIQNVRR225 pKa = 11.84 NYY227 pKa = 11.07 NVFTTYY233 pKa = 11.2 DD234 pKa = 3.23 PGARR238 pKa = 11.84 GLGMFGNANFTVDD251 pKa = 3.64 QSISLARR258 pKa = 11.84 SVTPISGLQGDD269 pKa = 4.58 TVTYY273 pKa = 10.48 SYY275 pKa = 10.76 TVTNNGEE282 pKa = 3.99 LPFNIGQDD290 pKa = 3.65 VVIEE294 pKa = 4.44 DD295 pKa = 4.56 DD296 pKa = 3.98 LLGSVTCPAITAPIAPGGTVTCTAPYY322 pKa = 9.01 TISALDD328 pKa = 3.65 VLTGTVNSTATAGIGAIGQAFSARR352 pKa = 11.84 LQSNSQSLSLVTSVLSGDD370 pKa = 4.31 SGAQNCTPQSVFAHH384 pKa = 5.84 TRR386 pKa = 11.84 SQLAGPGSAAALTTSDD402 pKa = 3.72 IFLFDD407 pKa = 5.34 DD408 pKa = 3.37 VTEE411 pKa = 4.53 DD412 pKa = 3.58 VNGNAIDD419 pKa = 4.08 VVFQLDD425 pKa = 3.73 QISNASAVKK434 pKa = 10.8 LEE436 pKa = 4.37 GSLQALMTPSNNGYY450 pKa = 7.48 VTYY453 pKa = 10.66 RR454 pKa = 11.84 LRR456 pKa = 11.84 LVQDD460 pKa = 4.11 GTATPANPQGIAIEE474 pKa = 3.93 QSRR477 pKa = 11.84 INGVIVQQTDD487 pKa = 2.56 VDD489 pKa = 4.23 SRR491 pKa = 11.84 GSSDD495 pKa = 3.83 DD496 pKa = 3.48 SSDD499 pKa = 3.39 VVGPVSSPRR508 pKa = 11.84 AISYY512 pKa = 10.77 LNTAPLVAFPAPGQAIAMDD531 pKa = 4.42 PAKK534 pKa = 10.75 QGDD537 pKa = 3.72 PSNWIDD543 pKa = 4.37 EE544 pKa = 4.47 PNEE547 pKa = 4.21 TNFDD551 pKa = 3.38 NYY553 pKa = 9.98 ATYY556 pKa = 10.19 EE557 pKa = 4.0 YY558 pKa = 9.64 DD559 pKa = 3.48 TFIEE563 pKa = 4.72 GAFIHH568 pKa = 6.84 GFTGSSTNPATRR580 pKa = 11.84 GSGILLCGIVNSSADD595 pKa = 3.56 VIARR599 pKa = 11.84 DD600 pKa = 4.07 DD601 pKa = 5.03 DD602 pKa = 4.3 YY603 pKa = 11.51 TASPLNSLLGGTAGEE618 pKa = 4.22 VLVNDD623 pKa = 5.5 TINALPALFPTATLEE638 pKa = 4.2 VLTPAVPQNDD648 pKa = 3.96 GDD650 pKa = 4.1 PVPVLEE656 pKa = 4.56 TSGVDD661 pKa = 2.98 AGRR664 pKa = 11.84 VIVPPGVPAGTYY676 pKa = 8.92 TIDD679 pKa = 3.8 YY680 pKa = 8.86 RR681 pKa = 11.84 LCDD684 pKa = 3.47 SLTPGDD690 pKa = 3.98 CDD692 pKa = 3.28 RR693 pKa = 11.84 AKK695 pKa = 10.03 VTLAVFDD702 pKa = 4.1 GLGLDD707 pKa = 4.32 FGDD710 pKa = 4.51 APVTYY715 pKa = 10.06 LVAAHH720 pKa = 7.05 AVRR723 pKa = 11.84 PSPTIYY729 pKa = 10.22 LGSVAPDD736 pKa = 3.29 IEE738 pKa = 4.52 IVAQSDD744 pKa = 3.41 ATATADD750 pKa = 4.96 DD751 pKa = 5.76 LIDD754 pKa = 4.88 NDD756 pKa = 5.19 DD757 pKa = 3.63 EE758 pKa = 4.55 DD759 pKa = 6.91 AILFPVMTQGAISTLNIPVTGAGVLQAWIDD789 pKa = 3.85 FNGDD793 pKa = 3.2 GVFEE797 pKa = 4.23 EE798 pKa = 4.64 TLGEE802 pKa = 4.43 RR803 pKa = 11.84 IASDD807 pKa = 3.94 LSDD810 pKa = 4.79 DD811 pKa = 3.69 GTGFDD816 pKa = 3.94 TVAGDD821 pKa = 3.69 GVIQVDD827 pKa = 3.87 VAVPTDD833 pKa = 3.2 ATTATTYY840 pKa = 11.35 ARR842 pKa = 11.84 FRR844 pKa = 11.84 YY845 pKa = 9.66 ASTPGLPAAGFALDD859 pKa = 5.22 GEE861 pKa = 4.69 VEE863 pKa = 4.54 DD864 pKa = 4.27 YY865 pKa = 11.55 SLVIVAADD873 pKa = 3.44 LVDD876 pKa = 5.01 RR877 pKa = 11.84 GDD879 pKa = 3.89 APASYY884 pKa = 10.51 GDD886 pKa = 3.63 PRR888 pKa = 11.84 HH889 pKa = 6.06 IVVPSIYY896 pKa = 10.26 LGAGLPDD903 pKa = 4.2 TEE905 pKa = 4.4 WVPQNSVTADD915 pKa = 3.54 ADD917 pKa = 3.91 DD918 pKa = 4.44 LAGSDD923 pKa = 4.79 DD924 pKa = 4.01 EE925 pKa = 5.39 DD926 pKa = 6.34 AIALFPVLEE935 pKa = 4.95 AGTTQNLTVQTHH947 pKa = 5.04 EE948 pKa = 4.25 TLSLQLALGLPVLSGITNLQLWVDD972 pKa = 3.78 WDD974 pKa = 3.56 QNGRR978 pKa = 11.84 FEE980 pKa = 4.23 TSEE983 pKa = 3.9 QVAVNYY989 pKa = 10.09 RR990 pKa = 11.84 DD991 pKa = 3.57 GGAGDD996 pKa = 3.58 TDD998 pKa = 3.83 GVFNNQISLEE1008 pKa = 4.1 IPVPGDD1014 pKa = 2.73 IGNGISYY1021 pKa = 10.9 ARR1023 pKa = 11.84 VRR1025 pKa = 11.84 WSTTSIVGLEE1035 pKa = 4.18 PFDD1038 pKa = 4.1 GLNLDD1043 pKa = 4.81 GEE1045 pKa = 4.67 VEE1047 pKa = 4.5 DD1048 pKa = 4.83 YY1049 pKa = 11.25 LVTLSNPNAPLVCDD1063 pKa = 3.13 TGFYY1067 pKa = 9.81 MIYY1070 pKa = 10.34 EE1071 pKa = 4.42 SGAQPILEE1079 pKa = 4.43 KK1080 pKa = 10.97 LQISGSAGSYY1090 pKa = 7.34 STNGTTYY1097 pKa = 9.62 PANFSGTYY1105 pKa = 9.18 TMSGWGWNEE1114 pKa = 3.16 VDD1116 pKa = 3.39 NYY1118 pKa = 10.4 IYY1120 pKa = 10.82 GIFRR1124 pKa = 11.84 NTFQLHH1130 pKa = 5.19 RR1131 pKa = 11.84 VTYY1134 pKa = 9.13 SGAVQLVSDD1143 pKa = 4.29 MSALGLEE1150 pKa = 4.53 PVGTTLEE1157 pKa = 4.0 ILPNGVLIYY1166 pKa = 10.58 NSQATTGRR1174 pKa = 11.84 YY1175 pKa = 7.83 QLVDD1179 pKa = 3.25 ISDD1182 pKa = 3.98 PSAPTNLGVLEE1193 pKa = 5.07 AGSSAPNGLDD1203 pKa = 2.93 MAYY1206 pKa = 10.52 NPRR1209 pKa = 11.84 DD1210 pKa = 3.18 GLLYY1214 pKa = 10.06 MVANGNDD1221 pKa = 3.43 IYY1223 pKa = 11.53 ALDD1226 pKa = 4.28 PLGGVAGATSTVLVADD1242 pKa = 4.14 NVTLPAGTGSYY1253 pKa = 10.51 QLDD1256 pKa = 4.1 SVWMDD1261 pKa = 3.19 EE1262 pKa = 3.79 NGYY1265 pKa = 10.33 FYY1267 pKa = 10.66 AYY1269 pKa = 10.63 DD1270 pKa = 3.62 NVSQQIFALEE1280 pKa = 3.96 LGEE1283 pKa = 5.26 AGDD1286 pKa = 4.52 RR1287 pKa = 11.84 PASFQFFKK1295 pKa = 11.26 VDD1297 pKa = 3.74 GTGSAQIGNDD1307 pKa = 3.36 GASCRR1312 pKa = 11.84 KK1313 pKa = 9.27 GSFYY1317 pKa = 11.08 ASTVFAEE1324 pKa = 4.73 GTISGRR1330 pKa = 11.84 LYY1332 pKa = 10.58 HH1333 pKa = 7.35 DD1334 pKa = 4.7 SNASGAYY1341 pKa = 9.61 DD1342 pKa = 3.39 VGEE1345 pKa = 4.5 SGLPADD1351 pKa = 4.62 ISMALYY1357 pKa = 10.23 RR1358 pKa = 11.84 DD1359 pKa = 3.46 NATPADD1365 pKa = 4.18 LTDD1368 pKa = 5.78 DD1369 pKa = 3.61 ILVTTTQTDD1378 pKa = 3.18 GDD1380 pKa = 3.81 GRR1382 pKa = 11.84 YY1383 pKa = 9.46 VFAGVDD1389 pKa = 3.19 ATVTYY1394 pKa = 10.35 RR1395 pKa = 11.84 IEE1397 pKa = 4.09 VNEE1400 pKa = 4.65 ADD1402 pKa = 4.18 PEE1404 pKa = 4.23 IPANRR1409 pKa = 11.84 TISTPNPRR1417 pKa = 11.84 IGVTVMTGAEE1427 pKa = 4.15 TSDD1430 pKa = 3.13 QDD1432 pKa = 4.14 FGFEE1436 pKa = 3.9 ATALQADD1443 pKa = 4.54 LSLNKK1448 pKa = 10.14 AAFDD1452 pKa = 3.67 TSGAALSSAQDD1463 pKa = 3.44 GAQIDD1468 pKa = 4.03 FVLTVTNDD1476 pKa = 3.44 GPATATDD1483 pKa = 3.36 VRR1485 pKa = 11.84 VRR1487 pKa = 11.84 DD1488 pKa = 4.61 LIPDD1492 pKa = 4.18 GFTYY1496 pKa = 10.7 VSNDD1500 pKa = 2.83 AAAQAMGYY1508 pKa = 10.06 DD1509 pKa = 4.0 PSSGIWTLTDD1519 pKa = 3.87 LPDD1522 pKa = 3.88 GTSATLTLRR1531 pKa = 11.84 VVMKK1535 pKa = 10.1 ATGTHH1540 pKa = 5.99 TNTAEE1545 pKa = 4.16 IVASAIPDD1553 pKa = 4.14 PDD1555 pKa = 3.69 SDD1557 pKa = 4.16 PAVGTLRR1564 pKa = 11.84 DD1565 pKa = 4.19 DD1566 pKa = 4.17 LSDD1569 pKa = 4.93 GIVDD1573 pKa = 4.55 DD1574 pKa = 5.49 DD1575 pKa = 4.11 EE1576 pKa = 7.04 ASATVSFIGMGPTLSGTVFRR1596 pKa = 11.84 DD1597 pKa = 3.0 NGAAGDD1603 pKa = 3.73 TAYY1606 pKa = 10.77 DD1607 pKa = 3.95 GLQGAQEE1614 pKa = 4.16 PGIPDD1619 pKa = 3.35 ATVTVADD1626 pKa = 3.78 TVGTVLGQPVVAADD1640 pKa = 4.13 GTWSLTLPEE1649 pKa = 4.85 GFNSAVTVTLAPTSDD1664 pKa = 3.39 GQVVSEE1670 pKa = 4.99 SPGTLPGLVNTQARR1684 pKa = 11.84 DD1685 pKa = 3.29 GSFGFTPVSGGTYY1698 pKa = 9.86 SNLNFGVIPSARR1710 pKa = 11.84 LRR1712 pKa = 11.84 NSQQAAMRR1720 pKa = 11.84 SGQVITLRR1728 pKa = 11.84 HH1729 pKa = 6.5 EE1730 pKa = 4.29 YY1731 pKa = 8.85 TAQAPGNVTFEE1742 pKa = 4.33 IDD1744 pKa = 3.28 VHH1746 pKa = 5.35 QATMPGLFSTGIFLDD1761 pKa = 4.29 PACNGTPITAVNGAQPITADD1781 pKa = 3.3 TRR1783 pKa = 11.84 ICLVIRR1789 pKa = 11.84 VTASSAASPGASIAFDD1805 pKa = 4.86 LIADD1809 pKa = 4.13 TTYY1812 pKa = 11.4 GATGLSAQVRR1822 pKa = 11.84 NTDD1825 pKa = 3.2 VVRR1828 pKa = 11.84 VDD1830 pKa = 3.53 SSQGQLTLRR1839 pKa = 11.84 KK1840 pKa = 7.12 TVRR1843 pKa = 11.84 NITQGTPEE1851 pKa = 4.27 GVSNGAALGDD1861 pKa = 3.56 VLEE1864 pKa = 4.03 YY1865 pKa = 10.38 RR1866 pKa = 11.84 IYY1868 pKa = 10.99 LDD1870 pKa = 4.36 NPGTLPATQIVIHH1883 pKa = 7.11 DD1884 pKa = 4.06 RR1885 pKa = 11.84 TPPYY1889 pKa = 7.57 TTLAAPIPSSTAIGTDD1905 pKa = 3.87 VICTLAVPSPNNAGYY1920 pKa = 10.53 AGPLRR1925 pKa = 11.84 WEE1927 pKa = 4.7 CAGTHH1932 pKa = 6.22 PPGATGSVTFQVQIAPP1948 pKa = 3.74
Molecular weight: 202.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.671
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.503
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.401
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.706
Sillero 3.872
Patrickios 1.405
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.784
Protein with the highest isoelectric point:
>tr|A0A1C2H9L7|A0A1C2H9L7_9RHOB Uncharacterized protein OS=Thioclava sp. SK-1 OX=1889770 GN=BFP70_17735 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.12 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.0 GRR39 pKa = 11.84 KK40 pKa = 8.91 SLSAA44 pKa = 3.86
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3819
0
3819
1238436
32
17860
324.3
35.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.189 ± 0.055
0.928 ± 0.017
6.188 ± 0.051
5.246 ± 0.054
3.641 ± 0.037
8.483 ± 0.064
2.127 ± 0.026
5.412 ± 0.027
3.149 ± 0.044
10.045 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.878 ± 0.03
2.668 ± 0.023
5.028 ± 0.045
3.827 ± 0.031
6.399 ± 0.052
5.388 ± 0.036
5.697 ± 0.065
7.125 ± 0.036
1.367 ± 0.018
2.215 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here