Thioclava sp. SK-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thioclava; unclassified Thioclava

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1C2HNN6|A0A1C2HNN6_9RHOB Peptidase_M48 domain-containing protein OS=Thioclava sp. SK-1 OX=1889770 GN=BFP70_09865 PE=3 SV=1
MM1 pKa = 7.74AILWGLGGPAAHH13 pKa = 7.12AGDD16 pKa = 4.51FQIDD20 pKa = 3.34WEE22 pKa = 4.37AFDD25 pKa = 3.98WPAGVTGPLVRR36 pKa = 11.84TLRR39 pKa = 11.84DD40 pKa = 3.36QNGFEE45 pKa = 4.08VDD47 pKa = 3.45VTVTHH52 pKa = 6.95TGPFAGYY59 pKa = 8.93PDD61 pKa = 4.31GAGGTIPTPDD71 pKa = 3.92DD72 pKa = 3.25VTIFGGSTEE81 pKa = 4.12SLILVVDD88 pKa = 4.58APQNQGAIGDD98 pKa = 3.96SRR100 pKa = 11.84TISTVSASSGGIAILVDD117 pKa = 4.05DD118 pKa = 4.51LTIDD122 pKa = 3.61VFDD125 pKa = 5.55IDD127 pKa = 4.01STDD130 pKa = 3.3NNAVSDD136 pKa = 3.84RR137 pKa = 11.84CDD139 pKa = 3.3FLTVFGDD146 pKa = 3.47NGNPTLSTLSLTPSVVVGPGFGSGLTGPITANQAQCIYY184 pKa = 10.77FEE186 pKa = 5.12GPTGSPTSPNDD197 pKa = 3.17DD198 pKa = 3.76TGSVRR203 pKa = 11.84ATFPDD208 pKa = 3.39ATSSVTFWYY217 pKa = 10.15DD218 pKa = 3.06EE219 pKa = 4.41SIQNVRR225 pKa = 11.84NYY227 pKa = 11.07NVFTTYY233 pKa = 11.2DD234 pKa = 3.23PGARR238 pKa = 11.84GLGMFGNANFTVDD251 pKa = 3.64QSISLARR258 pKa = 11.84SVTPISGLQGDD269 pKa = 4.58TVTYY273 pKa = 10.48SYY275 pKa = 10.76TVTNNGEE282 pKa = 3.99LPFNIGQDD290 pKa = 3.65VVIEE294 pKa = 4.44DD295 pKa = 4.56DD296 pKa = 3.98LLGSVTCPAITAPIAPGGTVTCTAPYY322 pKa = 9.01TISALDD328 pKa = 3.65VLTGTVNSTATAGIGAIGQAFSARR352 pKa = 11.84LQSNSQSLSLVTSVLSGDD370 pKa = 4.31SGAQNCTPQSVFAHH384 pKa = 5.84TRR386 pKa = 11.84SQLAGPGSAAALTTSDD402 pKa = 3.72IFLFDD407 pKa = 5.34DD408 pKa = 3.37VTEE411 pKa = 4.53DD412 pKa = 3.58VNGNAIDD419 pKa = 4.08VVFQLDD425 pKa = 3.73QISNASAVKK434 pKa = 10.8LEE436 pKa = 4.37GSLQALMTPSNNGYY450 pKa = 7.48VTYY453 pKa = 10.66RR454 pKa = 11.84LRR456 pKa = 11.84LVQDD460 pKa = 4.11GTATPANPQGIAIEE474 pKa = 3.93QSRR477 pKa = 11.84INGVIVQQTDD487 pKa = 2.56VDD489 pKa = 4.23SRR491 pKa = 11.84GSSDD495 pKa = 3.83DD496 pKa = 3.48SSDD499 pKa = 3.39VVGPVSSPRR508 pKa = 11.84AISYY512 pKa = 10.77LNTAPLVAFPAPGQAIAMDD531 pKa = 4.42PAKK534 pKa = 10.75QGDD537 pKa = 3.72PSNWIDD543 pKa = 4.37EE544 pKa = 4.47PNEE547 pKa = 4.21TNFDD551 pKa = 3.38NYY553 pKa = 9.98ATYY556 pKa = 10.19EE557 pKa = 4.0YY558 pKa = 9.64DD559 pKa = 3.48TFIEE563 pKa = 4.72GAFIHH568 pKa = 6.84GFTGSSTNPATRR580 pKa = 11.84GSGILLCGIVNSSADD595 pKa = 3.56VIARR599 pKa = 11.84DD600 pKa = 4.07DD601 pKa = 5.03DD602 pKa = 4.3YY603 pKa = 11.51TASPLNSLLGGTAGEE618 pKa = 4.22VLVNDD623 pKa = 5.5TINALPALFPTATLEE638 pKa = 4.2VLTPAVPQNDD648 pKa = 3.96GDD650 pKa = 4.1PVPVLEE656 pKa = 4.56TSGVDD661 pKa = 2.98AGRR664 pKa = 11.84VIVPPGVPAGTYY676 pKa = 8.92TIDD679 pKa = 3.8YY680 pKa = 8.86RR681 pKa = 11.84LCDD684 pKa = 3.47SLTPGDD690 pKa = 3.98CDD692 pKa = 3.28RR693 pKa = 11.84AKK695 pKa = 10.03VTLAVFDD702 pKa = 4.1GLGLDD707 pKa = 4.32FGDD710 pKa = 4.51APVTYY715 pKa = 10.06LVAAHH720 pKa = 7.05AVRR723 pKa = 11.84PSPTIYY729 pKa = 10.22LGSVAPDD736 pKa = 3.29IEE738 pKa = 4.52IVAQSDD744 pKa = 3.41ATATADD750 pKa = 4.96DD751 pKa = 5.76LIDD754 pKa = 4.88NDD756 pKa = 5.19DD757 pKa = 3.63EE758 pKa = 4.55DD759 pKa = 6.91AILFPVMTQGAISTLNIPVTGAGVLQAWIDD789 pKa = 3.85FNGDD793 pKa = 3.2GVFEE797 pKa = 4.23EE798 pKa = 4.64TLGEE802 pKa = 4.43RR803 pKa = 11.84IASDD807 pKa = 3.94LSDD810 pKa = 4.79DD811 pKa = 3.69GTGFDD816 pKa = 3.94TVAGDD821 pKa = 3.69GVIQVDD827 pKa = 3.87VAVPTDD833 pKa = 3.2ATTATTYY840 pKa = 11.35ARR842 pKa = 11.84FRR844 pKa = 11.84YY845 pKa = 9.66ASTPGLPAAGFALDD859 pKa = 5.22GEE861 pKa = 4.69VEE863 pKa = 4.54DD864 pKa = 4.27YY865 pKa = 11.55SLVIVAADD873 pKa = 3.44LVDD876 pKa = 5.01RR877 pKa = 11.84GDD879 pKa = 3.89APASYY884 pKa = 10.51GDD886 pKa = 3.63PRR888 pKa = 11.84HH889 pKa = 6.06IVVPSIYY896 pKa = 10.26LGAGLPDD903 pKa = 4.2TEE905 pKa = 4.4WVPQNSVTADD915 pKa = 3.54ADD917 pKa = 3.91DD918 pKa = 4.44LAGSDD923 pKa = 4.79DD924 pKa = 4.01EE925 pKa = 5.39DD926 pKa = 6.34AIALFPVLEE935 pKa = 4.95AGTTQNLTVQTHH947 pKa = 5.04EE948 pKa = 4.25TLSLQLALGLPVLSGITNLQLWVDD972 pKa = 3.78WDD974 pKa = 3.56QNGRR978 pKa = 11.84FEE980 pKa = 4.23TSEE983 pKa = 3.9QVAVNYY989 pKa = 10.09RR990 pKa = 11.84DD991 pKa = 3.57GGAGDD996 pKa = 3.58TDD998 pKa = 3.83GVFNNQISLEE1008 pKa = 4.1IPVPGDD1014 pKa = 2.73IGNGISYY1021 pKa = 10.9ARR1023 pKa = 11.84VRR1025 pKa = 11.84WSTTSIVGLEE1035 pKa = 4.18PFDD1038 pKa = 4.1GLNLDD1043 pKa = 4.81GEE1045 pKa = 4.67VEE1047 pKa = 4.5DD1048 pKa = 4.83YY1049 pKa = 11.25LVTLSNPNAPLVCDD1063 pKa = 3.13TGFYY1067 pKa = 9.81MIYY1070 pKa = 10.34EE1071 pKa = 4.42SGAQPILEE1079 pKa = 4.43KK1080 pKa = 10.97LQISGSAGSYY1090 pKa = 7.34STNGTTYY1097 pKa = 9.62PANFSGTYY1105 pKa = 9.18TMSGWGWNEE1114 pKa = 3.16VDD1116 pKa = 3.39NYY1118 pKa = 10.4IYY1120 pKa = 10.82GIFRR1124 pKa = 11.84NTFQLHH1130 pKa = 5.19RR1131 pKa = 11.84VTYY1134 pKa = 9.13SGAVQLVSDD1143 pKa = 4.29MSALGLEE1150 pKa = 4.53PVGTTLEE1157 pKa = 4.0ILPNGVLIYY1166 pKa = 10.58NSQATTGRR1174 pKa = 11.84YY1175 pKa = 7.83QLVDD1179 pKa = 3.25ISDD1182 pKa = 3.98PSAPTNLGVLEE1193 pKa = 5.07AGSSAPNGLDD1203 pKa = 2.93MAYY1206 pKa = 10.52NPRR1209 pKa = 11.84DD1210 pKa = 3.18GLLYY1214 pKa = 10.06MVANGNDD1221 pKa = 3.43IYY1223 pKa = 11.53ALDD1226 pKa = 4.28PLGGVAGATSTVLVADD1242 pKa = 4.14NVTLPAGTGSYY1253 pKa = 10.51QLDD1256 pKa = 4.1SVWMDD1261 pKa = 3.19EE1262 pKa = 3.79NGYY1265 pKa = 10.33FYY1267 pKa = 10.66AYY1269 pKa = 10.63DD1270 pKa = 3.62NVSQQIFALEE1280 pKa = 3.96LGEE1283 pKa = 5.26AGDD1286 pKa = 4.52RR1287 pKa = 11.84PASFQFFKK1295 pKa = 11.26VDD1297 pKa = 3.74GTGSAQIGNDD1307 pKa = 3.36GASCRR1312 pKa = 11.84KK1313 pKa = 9.27GSFYY1317 pKa = 11.08ASTVFAEE1324 pKa = 4.73GTISGRR1330 pKa = 11.84LYY1332 pKa = 10.58HH1333 pKa = 7.35DD1334 pKa = 4.7SNASGAYY1341 pKa = 9.61DD1342 pKa = 3.39VGEE1345 pKa = 4.5SGLPADD1351 pKa = 4.62ISMALYY1357 pKa = 10.23RR1358 pKa = 11.84DD1359 pKa = 3.46NATPADD1365 pKa = 4.18LTDD1368 pKa = 5.78DD1369 pKa = 3.61ILVTTTQTDD1378 pKa = 3.18GDD1380 pKa = 3.81GRR1382 pKa = 11.84YY1383 pKa = 9.46VFAGVDD1389 pKa = 3.19ATVTYY1394 pKa = 10.35RR1395 pKa = 11.84IEE1397 pKa = 4.09VNEE1400 pKa = 4.65ADD1402 pKa = 4.18PEE1404 pKa = 4.23IPANRR1409 pKa = 11.84TISTPNPRR1417 pKa = 11.84IGVTVMTGAEE1427 pKa = 4.15TSDD1430 pKa = 3.13QDD1432 pKa = 4.14FGFEE1436 pKa = 3.9ATALQADD1443 pKa = 4.54LSLNKK1448 pKa = 10.14AAFDD1452 pKa = 3.67TSGAALSSAQDD1463 pKa = 3.44GAQIDD1468 pKa = 4.03FVLTVTNDD1476 pKa = 3.44GPATATDD1483 pKa = 3.36VRR1485 pKa = 11.84VRR1487 pKa = 11.84DD1488 pKa = 4.61LIPDD1492 pKa = 4.18GFTYY1496 pKa = 10.7VSNDD1500 pKa = 2.83AAAQAMGYY1508 pKa = 10.06DD1509 pKa = 4.0PSSGIWTLTDD1519 pKa = 3.87LPDD1522 pKa = 3.88GTSATLTLRR1531 pKa = 11.84VVMKK1535 pKa = 10.1ATGTHH1540 pKa = 5.99TNTAEE1545 pKa = 4.16IVASAIPDD1553 pKa = 4.14PDD1555 pKa = 3.69SDD1557 pKa = 4.16PAVGTLRR1564 pKa = 11.84DD1565 pKa = 4.19DD1566 pKa = 4.17LSDD1569 pKa = 4.93GIVDD1573 pKa = 4.55DD1574 pKa = 5.49DD1575 pKa = 4.11EE1576 pKa = 7.04ASATVSFIGMGPTLSGTVFRR1596 pKa = 11.84DD1597 pKa = 3.0NGAAGDD1603 pKa = 3.73TAYY1606 pKa = 10.77DD1607 pKa = 3.95GLQGAQEE1614 pKa = 4.16PGIPDD1619 pKa = 3.35ATVTVADD1626 pKa = 3.78TVGTVLGQPVVAADD1640 pKa = 4.13GTWSLTLPEE1649 pKa = 4.85GFNSAVTVTLAPTSDD1664 pKa = 3.39GQVVSEE1670 pKa = 4.99SPGTLPGLVNTQARR1684 pKa = 11.84DD1685 pKa = 3.29GSFGFTPVSGGTYY1698 pKa = 9.86SNLNFGVIPSARR1710 pKa = 11.84LRR1712 pKa = 11.84NSQQAAMRR1720 pKa = 11.84SGQVITLRR1728 pKa = 11.84HH1729 pKa = 6.5EE1730 pKa = 4.29YY1731 pKa = 8.85TAQAPGNVTFEE1742 pKa = 4.33IDD1744 pKa = 3.28VHH1746 pKa = 5.35QATMPGLFSTGIFLDD1761 pKa = 4.29PACNGTPITAVNGAQPITADD1781 pKa = 3.3TRR1783 pKa = 11.84ICLVIRR1789 pKa = 11.84VTASSAASPGASIAFDD1805 pKa = 4.86LIADD1809 pKa = 4.13TTYY1812 pKa = 11.4GATGLSAQVRR1822 pKa = 11.84NTDD1825 pKa = 3.2VVRR1828 pKa = 11.84VDD1830 pKa = 3.53SSQGQLTLRR1839 pKa = 11.84KK1840 pKa = 7.12TVRR1843 pKa = 11.84NITQGTPEE1851 pKa = 4.27GVSNGAALGDD1861 pKa = 3.56VLEE1864 pKa = 4.03YY1865 pKa = 10.38RR1866 pKa = 11.84IYY1868 pKa = 10.99LDD1870 pKa = 4.36NPGTLPATQIVIHH1883 pKa = 7.11DD1884 pKa = 4.06RR1885 pKa = 11.84TPPYY1889 pKa = 7.57TTLAAPIPSSTAIGTDD1905 pKa = 3.87VICTLAVPSPNNAGYY1920 pKa = 10.53AGPLRR1925 pKa = 11.84WEE1927 pKa = 4.7CAGTHH1932 pKa = 6.22PPGATGSVTFQVQIAPP1948 pKa = 3.74

Molecular weight:
202.22 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1C2H9L7|A0A1C2H9L7_9RHOB Uncharacterized protein OS=Thioclava sp. SK-1 OX=1889770 GN=BFP70_17735 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.12GGRR28 pKa = 11.84RR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.0GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight:
5.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3819

0

3819

1238436

32

17860

324.3

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.189 ± 0.055

0.928 ± 0.017

6.188 ± 0.051

5.246 ± 0.054

3.641 ± 0.037

8.483 ± 0.064

2.127 ± 0.026

5.412 ± 0.027

3.149 ± 0.044

10.045 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.878 ± 0.03

2.668 ± 0.023

5.028 ± 0.045

3.827 ± 0.031

6.399 ± 0.052

5.388 ± 0.036

5.697 ± 0.065

7.125 ± 0.036

1.367 ± 0.018

2.215 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski