Amino acid dipepetide frequency for Psilocybe cyanescens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.799AlaAla: 7.799 ± 0.057	0.989AlaCys: 0.989 ± 0.013	3.882AlaAsp: 3.882 ± 0.03	4.309AlaGlu: 4.309 ± 0.035	3.21AlaPhe: 3.21 ± 0.026	5.211AlaGly: 5.211 ± 0.039	1.968AlaHis: 1.968 ± 0.019	4.3AlaIle: 4.3 ± 0.03	3.671AlaLys: 3.671 ± 0.029	7.801AlaLeu: 7.801 ± 0.047	1.739AlaMet: 1.739 ± 0.017	3.067AlaAsn: 3.067 ± 0.025	4.781AlaPro: 4.781 ± 0.038	3.172AlaGln: 3.172 ± 0.029	4.475AlaArg: 4.475 ± 0.029	7.548AlaSer: 7.548 ± 0.042	5.084AlaThr: 5.084 ± 0.032	5.092AlaVal: 5.092 ± 0.035	1.027AlaTrp: 1.027 ± 0.014	2.164AlaTyr: 2.164 ± 0.017	0.004AlaXaa: 0.004 ± 0.001
Cys	0.895CysAla: 0.895 ± 0.012	0.246CysCys: 0.246 ± 0.007	0.645CysAsp: 0.645 ± 0.011	0.554CysGlu: 0.554 ± 0.01	0.51CysPhe: 0.51 ± 0.008	0.814CysGly: 0.814 ± 0.012	0.339CysHis: 0.339 ± 0.007	0.753CysIle: 0.753 ± 0.012	0.527CysLys: 0.527 ± 0.01	1.239CysLeu: 1.239 ± 0.015	0.273CysMet: 0.273 ± 0.008	0.451CysAsn: 0.451 ± 0.01	0.716CysPro: 0.716 ± 0.013	0.428CysGln: 0.428 ± 0.009	0.678CysArg: 0.678 ± 0.012	0.996CysSer: 0.996 ± 0.015	0.76CysThr: 0.76 ± 0.013	0.778CysVal: 0.778 ± 0.011	0.181CysTrp: 0.181 ± 0.005	0.328CysTyr: 0.328 ± 0.007	0.001CysXaa: 0.001 ± 0.0
Asp	4.391AspAla: 4.391 ± 0.03	0.605AspCys: 0.605 ± 0.01	4.251AspAsp: 4.251 ± 0.039	4.121AspGlu: 4.121 ± 0.032	2.065AspPhe: 2.065 ± 0.017	3.803AspGly: 3.803 ± 0.032	1.288AspHis: 1.288 ± 0.016	3.43AspIle: 3.43 ± 0.025	2.355AspLys: 2.355 ± 0.027	4.862AspLeu: 4.862 ± 0.033	1.216AspMet: 1.216 ± 0.014	2.122AspAsn: 2.122 ± 0.019	3.23AspPro: 3.23 ± 0.023	1.674AspGln: 1.674 ± 0.016	2.715AspArg: 2.715 ± 0.024	4.352AspSer: 4.352 ± 0.027	3.07AspThr: 3.07 ± 0.022	3.717AspVal: 3.717 ± 0.027	0.836AspTrp: 0.836 ± 0.012	1.461AspTyr: 1.461 ± 0.018	0.002AspXaa: 0.002 ± 0.001
Glu	4.522GluAla: 4.522 ± 0.033	0.64GluCys: 0.64 ± 0.011	4.129GluAsp: 4.129 ± 0.042	5.228GluGlu: 5.228 ± 0.058	1.801GluPhe: 1.801 ± 0.016	3.485GluGly: 3.485 ± 0.031	1.439GluHis: 1.439 ± 0.017	3.005GluIle: 3.005 ± 0.025	3.241GluLys: 3.241 ± 0.028	4.864GluLeu: 4.864 ± 0.034	1.283GluMet: 1.283 ± 0.016	2.135GluAsn: 2.135 ± 0.018	2.454GluPro: 2.454 ± 0.026	2.146GluGln: 2.146 ± 0.022	3.656GluArg: 3.656 ± 0.039	4.037GluSer: 4.037 ± 0.03	3.026GluThr: 3.026 ± 0.02	3.297GluVal: 3.297 ± 0.028	0.843GluTrp: 0.843 ± 0.012	1.589GluTyr: 1.589 ± 0.016	0.002GluXaa: 0.002 ± 0.001
Phe	2.926PheAla: 2.926 ± 0.026	0.577PheCys: 0.577 ± 0.01	2.267PheAsp: 2.267 ± 0.021	2.03PheGlu: 2.03 ± 0.023	1.745PhePhe: 1.745 ± 0.019	2.588PheGly: 2.588 ± 0.025	0.953PheHis: 0.953 ± 0.011	2.138PheIle: 2.138 ± 0.021	1.685PheLys: 1.685 ± 0.017	3.722PheLeu: 3.722 ± 0.031	0.793PheMet: 0.793 ± 0.01	1.641PheAsn: 1.641 ± 0.017	2.067PhePro: 2.067 ± 0.018	1.297PheGln: 1.297 ± 0.014	1.903PheArg: 1.903 ± 0.018	3.465PheSer: 3.465 ± 0.028	2.217PheThr: 2.217 ± 0.02	2.479PheVal: 2.479 ± 0.023	0.59PheTrp: 0.59 ± 0.01	1.1PheTyr: 1.1 ± 0.014	0.002PheXaa: 0.002 ± 0.001
Gly	4.821GlyAla: 4.821 ± 0.037	0.774GlyCys: 0.774 ± 0.012	3.256GlyAsp: 3.256 ± 0.025	3.27GlyGlu: 3.27 ± 0.031	2.458GlyPhe: 2.458 ± 0.022	5.628GlyGly: 5.628 ± 0.059	1.725GlyHis: 1.725 ± 0.02	3.272GlyIle: 3.272 ± 0.022	3.324GlyLys: 3.324 ± 0.029	5.432GlyLeu: 5.432 ± 0.031	1.466GlyMet: 1.466 ± 0.018	2.51GlyAsn: 2.51 ± 0.026	3.159GlyPro: 3.159 ± 0.028	2.307GlyGln: 2.307 ± 0.021	3.611GlyArg: 3.611 ± 0.028	5.646GlySer: 5.646 ± 0.037	3.896GlyThr: 3.896 ± 0.03	4.089GlyVal: 4.089 ± 0.031	1.015GlyTrp: 1.015 ± 0.014	1.881GlyTyr: 1.881 ± 0.019	0.003GlyXaa: 0.003 ± 0.001
His	1.995HisAla: 1.995 ± 0.018	0.36HisCys: 0.36 ± 0.008	1.368HisAsp: 1.368 ± 0.016	1.236HisGlu: 1.236 ± 0.014	1.03HisPhe: 1.03 ± 0.012	1.582HisGly: 1.582 ± 0.018	0.984HisHis: 0.984 ± 0.017	1.564HisIle: 1.564 ± 0.016	0.958HisLys: 0.958 ± 0.012	2.504HisLeu: 2.504 ± 0.022	0.506HisMet: 0.506 ± 0.008	1.003HisAsn: 1.003 ± 0.014	1.985HisPro: 1.985 ± 0.022	1.016HisGln: 1.016 ± 0.016	1.595HisArg: 1.595 ± 0.017	2.288HisSer: 2.288 ± 0.02	1.535HisThr: 1.535 ± 0.015	1.53HisVal: 1.53 ± 0.016	0.327HisTrp: 0.327 ± 0.006	0.705HisTyr: 0.705 ± 0.011	0.001HisXaa: 0.001 ± 0.0
Ile	4.276IleAla: 4.276 ± 0.029	0.783IleCys: 0.783 ± 0.013	2.988IleAsp: 2.988 ± 0.024	2.783IleGlu: 2.783 ± 0.022	2.249IlePhe: 2.249 ± 0.021	3.058IleGly: 3.058 ± 0.023	1.382IleHis: 1.382 ± 0.015	2.917IleIle: 2.917 ± 0.022	2.284IleLys: 2.284 ± 0.02	5.223IleLeu: 5.223 ± 0.034	1.082IleMet: 1.082 ± 0.013	2.023IleAsn: 2.023 ± 0.019	3.545IlePro: 3.545 ± 0.024	2.025IleGln: 2.025 ± 0.018	2.85IleArg: 2.85 ± 0.022	4.692IleSer: 4.692 ± 0.027	3.148IleThr: 3.148 ± 0.024	3.502IleVal: 3.502 ± 0.027	0.766IleTrp: 0.766 ± 0.012	1.495IleTyr: 1.495 ± 0.015	0.002IleXaa: 0.002 ± 0.0
Lys	4.109LysAla: 4.109 ± 0.031	0.512LysCys: 0.512 ± 0.01	2.781LysAsp: 2.781 ± 0.026	3.263LysGlu: 3.263 ± 0.03	1.556LysPhe: 1.556 ± 0.016	2.84LysGly: 2.84 ± 0.028	1.182LysHis: 1.182 ± 0.016	2.43LysIle: 2.43 ± 0.02	3.254LysLys: 3.254 ± 0.033	3.997LysLeu: 3.997 ± 0.029	0.992LysMet: 0.992 ± 0.013	1.77LysAsn: 1.77 ± 0.019	2.545LysPro: 2.545 ± 0.024	1.667LysGln: 1.667 ± 0.015	3.182LysArg: 3.182 ± 0.026	3.619LysSer: 3.619 ± 0.028	2.743LysThr: 2.743 ± 0.022	2.73LysVal: 2.73 ± 0.021	0.637LysTrp: 0.637 ± 0.01	1.292LysTyr: 1.292 ± 0.015	0.001LysXaa: 0.001 ± 0.0
Leu	7.68LeuAla: 7.68 ± 0.047	1.227LeuCys: 1.227 ± 0.016	5.087LeuAsp: 5.087 ± 0.029	5.24LeuGlu: 5.24 ± 0.037	3.535LeuPhe: 3.535 ± 0.031	5.343LeuGly: 5.343 ± 0.039	2.506LeuHis: 2.506 ± 0.023	4.346LeuIle: 4.346 ± 0.033	4.302LeuLys: 4.302 ± 0.026	8.63LeuLeu: 8.63 ± 0.062	1.755LeuMet: 1.755 ± 0.016	3.444LeuAsn: 3.444 ± 0.024	6.054LeuPro: 6.054 ± 0.036	3.755LeuGln: 3.755 ± 0.028	5.47LeuArg: 5.47 ± 0.032	8.336LeuSer: 8.336 ± 0.042	5.268LeuThr: 5.268 ± 0.034	5.652LeuVal: 5.652 ± 0.037	1.184LeuTrp: 1.184 ± 0.014	2.466LeuTyr: 2.466 ± 0.02	0.003LeuXaa: 0.003 ± 0.001
Met	1.823MetAla: 1.823 ± 0.015	0.24MetCys: 0.24 ± 0.006	1.26MetAsp: 1.26 ± 0.014	1.16MetGlu: 1.16 ± 0.013	0.775MetPhe: 0.775 ± 0.012	1.302MetGly: 1.302 ± 0.016	0.547MetHis: 0.547 ± 0.009	0.985MetIle: 0.985 ± 0.013	1.0MetLys: 1.0 ± 0.013	1.812MetLeu: 1.812 ± 0.018	0.492MetMet: 0.492 ± 0.009	0.815MetAsn: 0.815 ± 0.011	1.29MetPro: 1.29 ± 0.015	0.872MetGln: 0.872 ± 0.012	1.188MetArg: 1.188 ± 0.013	2.011MetSer: 2.011 ± 0.021	1.251MetThr: 1.251 ± 0.014	1.182MetVal: 1.182 ± 0.013	0.266MetTrp: 0.266 ± 0.006	0.541MetTyr: 0.541 ± 0.009	0.001MetXaa: 0.001 ± 0.0
Asn	3.284AsnAla: 3.284 ± 0.026	0.432AsnCys: 0.432 ± 0.009	2.113AsnAsp: 2.113 ± 0.021	1.956AsnGlu: 1.956 ± 0.019	1.462AsnPhe: 1.462 ± 0.017	2.783AsnGly: 2.783 ± 0.027	0.935AsnHis: 0.935 ± 0.013	2.348AsnIle: 2.348 ± 0.021	1.659AsnLys: 1.659 ± 0.018	3.571AsnLeu: 3.571 ± 0.025	0.851AsnMet: 0.851 ± 0.013	1.865AsnAsn: 1.865 ± 0.023	2.614AsnPro: 2.614 ± 0.021	1.328AsnGln: 1.328 ± 0.016	1.884AsnArg: 1.884 ± 0.017	3.234AsnSer: 3.234 ± 0.029	2.527AsnThr: 2.527 ± 0.023	2.561AsnVal: 2.561 ± 0.02	0.502AsnTrp: 0.502 ± 0.008	0.987AsnTyr: 0.987 ± 0.012	0.002AsnXaa: 0.002 ± 0.001
Pro	5.139ProAla: 5.139 ± 0.042	0.529ProCys: 0.529 ± 0.013	3.011ProAsp: 3.011 ± 0.021	3.324ProGlu: 3.324 ± 0.027	2.265ProPhe: 2.265 ± 0.02	3.548ProGly: 3.548 ± 0.029	1.597ProHis: 1.597 ± 0.016	2.984ProIle: 2.984 ± 0.022	2.549ProLys: 2.549 ± 0.023	5.41ProLeu: 5.41 ± 0.034	1.085ProMet: 1.085 ± 0.015	2.407ProAsn: 2.407 ± 0.023	6.223ProPro: 6.223 ± 0.07	2.435ProGln: 2.435 ± 0.03	3.252ProArg: 3.252 ± 0.027	7.216ProSer: 7.216 ± 0.055	4.564ProThr: 4.564 ± 0.031	3.756ProVal: 3.756 ± 0.031	0.669ProTrp: 0.669 ± 0.01	1.574ProTyr: 1.574 ± 0.017	0.003ProXaa: 0.003 ± 0.001
Gln	3.122GlnAla: 3.122 ± 0.025	0.467GlnCys: 0.467 ± 0.01	1.923GlnAsp: 1.923 ± 0.018	2.191GlnGlu: 2.191 ± 0.02	1.333GlnPhe: 1.333 ± 0.015	2.225GlnGly: 2.225 ± 0.023	1.171GlnHis: 1.171 ± 0.016	1.94GlnIle: 1.94 ± 0.017	1.805GlnLys: 1.805 ± 0.017	3.338GlnLeu: 3.338 ± 0.026	0.827GlnMet: 0.827 ± 0.012	1.518GlnAsn: 1.518 ± 0.018	2.383GlnPro: 2.383 ± 0.025	2.462GlnGln: 2.462 ± 0.064	2.331GlnArg: 2.331 ± 0.023	3.135GlnSer: 3.135 ± 0.025	2.343GlnThr: 2.343 ± 0.021	2.069GlnVal: 2.069 ± 0.017	0.515GlnTrp: 0.515 ± 0.01	1.12GlnTyr: 1.12 ± 0.014	0.001GlnXaa: 0.001 ± 0.0
Arg	4.318ArgAla: 4.318 ± 0.028	0.683ArgCys: 0.683 ± 0.012	3.013ArgAsp: 3.013 ± 0.029	3.466ArgGlu: 3.466 ± 0.04	2.075ArgPhe: 2.075 ± 0.021	3.259ArgGly: 3.259 ± 0.029	1.54ArgHis: 1.54 ± 0.015	3.025ArgIle: 3.025 ± 0.024	3.201ArgLys: 3.201 ± 0.028	5.146ArgLeu: 5.146 ± 0.031	1.274ArgMet: 1.274 ± 0.014	2.172ArgAsn: 2.172 ± 0.017	3.39ArgPro: 3.39 ± 0.03	2.26ArgGln: 2.26 ± 0.019	4.583ArgArg: 4.583 ± 0.036	4.889ArgSer: 4.889 ± 0.034	3.467ArgThr: 3.467 ± 0.029	3.375ArgVal: 3.375 ± 0.026	0.834ArgTrp: 0.834 ± 0.011	1.582ArgTyr: 1.582 ± 0.016	0.003ArgXaa: 0.003 ± 0.001
Ser	7.099SerAla: 7.099 ± 0.037	0.942SerCys: 0.942 ± 0.015	4.448SerAsp: 4.448 ± 0.03	4.11SerGlu: 4.11 ± 0.029	3.463SerPhe: 3.463 ± 0.023	5.792SerGly: 5.792 ± 0.039	2.274SerHis: 2.274 ± 0.019	4.563SerIle: 4.563 ± 0.027	3.904SerLys: 3.904 ± 0.027	8.17SerLeu: 8.17 ± 0.044	1.779SerMet: 1.779 ± 0.017	3.496SerAsn: 3.496 ± 0.025	6.277SerPro: 6.277 ± 0.055	3.292SerGln: 3.292 ± 0.029	5.172SerArg: 5.172 ± 0.04	11.295SerSer: 11.295 ± 0.091	6.555SerThr: 6.555 ± 0.044	5.216SerVal: 5.216 ± 0.032	1.075SerTrp: 1.075 ± 0.013	2.186SerTyr: 2.186 ± 0.02	0.002SerXaa: 0.002 ± 0.001
Thr	4.941ThrAla: 4.941 ± 0.034	0.733ThrCys: 0.733 ± 0.011	2.849ThrAsp: 2.849 ± 0.023	2.795ThrGlu: 2.795 ± 0.022	2.498ThrPhe: 2.498 ± 0.021	3.813ThrGly: 3.813 ± 0.031	1.57ThrHis: 1.57 ± 0.016	3.43ThrIle: 3.43 ± 0.025	2.584ThrLys: 2.584 ± 0.02	5.832ThrLeu: 5.832 ± 0.039	1.213ThrMet: 1.213 ± 0.013	2.326ThrAsn: 2.326 ± 0.021	4.82ThrPro: 4.82 ± 0.036	2.155ThrGln: 2.155 ± 0.02	3.382ThrArg: 3.382 ± 0.025	6.252ThrSer: 6.252 ± 0.034	4.707ThrThr: 4.707 ± 0.043	3.861ThrVal: 3.861 ± 0.027	0.809ThrTrp: 0.809 ± 0.01	1.636ThrTyr: 1.636 ± 0.019	0.004ThrXaa: 0.004 ± 0.001
Val	5.013ValAla: 5.013 ± 0.031	0.82ValCys: 0.82 ± 0.011	3.763ValAsp: 3.763 ± 0.023	3.649ValGlu: 3.649 ± 0.026	2.468ValPhe: 2.468 ± 0.023	3.818ValGly: 3.818 ± 0.033	1.58ValHis: 1.58 ± 0.017	3.321ValIle: 3.321 ± 0.026	2.851ValLys: 2.851 ± 0.025	5.983ValLeu: 5.983 ± 0.041	1.281ValMet: 1.281 ± 0.014	2.338ValAsn: 2.338 ± 0.02	3.854ValPro: 3.854 ± 0.03	2.431ValGln: 2.431 ± 0.019	3.216ValArg: 3.216 ± 0.025	4.874ValSer: 4.874 ± 0.03	3.496ValThr: 3.496 ± 0.024	4.494ValVal: 4.494 ± 0.032	0.874ValTrp: 0.874 ± 0.013	1.727ValTyr: 1.727 ± 0.017	0.003ValXaa: 0.003 ± 0.001
Trp	0.978TrpAla: 0.978 ± 0.012	0.184TrpCys: 0.184 ± 0.006	0.905TrpAsp: 0.905 ± 0.014	0.779TrpGlu: 0.779 ± 0.011	0.54TrpPhe: 0.54 ± 0.01	0.831TrpGly: 0.831 ± 0.012	0.315TrpHis: 0.315 ± 0.007	0.798TrpIle: 0.798 ± 0.013	0.817TrpLys: 0.817 ± 0.012	1.174TrpLeu: 1.174 ± 0.015	0.327TrpMet: 0.327 ± 0.007	0.654TrpAsn: 0.654 ± 0.011	0.562TrpPro: 0.562 ± 0.009	0.442TrpGln: 0.442 ± 0.01	0.856TrpArg: 0.856 ± 0.011	1.044TrpSer: 1.044 ± 0.014	0.885TrpThr: 0.885 ± 0.012	0.823TrpVal: 0.823 ± 0.012	0.268TrpTrp: 0.268 ± 0.007	0.4TrpTyr: 0.4 ± 0.009	0.001TrpXaa: 0.001 ± 0.0
Tyr	2.158TyrAla: 2.158 ± 0.02	0.37TyrCys: 0.37 ± 0.008	1.625TyrAsp: 1.625 ± 0.017	1.357TyrGlu: 1.357 ± 0.017	1.144TyrPhe: 1.144 ± 0.014	1.82TyrGly: 1.82 ± 0.019	0.776TyrHis: 0.776 ± 0.01	1.521TyrIle: 1.521 ± 0.015	1.13TyrLys: 1.13 ± 0.013	2.57TyrLeu: 2.57 ± 0.022	0.577TyrMet: 0.577 ± 0.009	1.139TyrAsn: 1.139 ± 0.012	1.591TyrPro: 1.591 ± 0.019	1.051TyrGln: 1.051 ± 0.012	1.551TyrArg: 1.551 ± 0.015	2.177TyrSer: 2.177 ± 0.018	1.666TyrThr: 1.666 ± 0.018	1.648TyrVal: 1.648 ± 0.017	0.363TyrTrp: 0.363 ± 0.007	0.891TyrTyr: 0.891 ± 0.013	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.003XaaLeu: 0.003 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.002XaaAsn: 0.002 ± 0.001	0.003XaaPro: 0.003 ± 0.001	0.002XaaGln: 0.002 ± 0.0	0.004XaaArg: 0.004 ± 0.001	0.004XaaSer: 0.004 ± 0.001	0.004XaaThr: 0.004 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 15931 proteins (6841066 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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