Streptococcus satellite phage Javan359
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKK1|A0A4D5ZKK1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan359 OX=2558659 GN=JavanS359_0010 PE=4 SV=1
MM1 pKa = 7.23 TVNTNDD7 pKa = 3.31 VLVDD11 pKa = 3.87 YY12 pKa = 10.78 EE13 pKa = 4.69 GLCCQLTDD21 pKa = 3.53 TLLVLEE27 pKa = 4.51 MASMEE32 pKa = 4.33 DD33 pKa = 3.64 SKK35 pKa = 11.49 QSSALLNTVIQAMNQLISEE54 pKa = 4.69 HH55 pKa = 5.24 TQQANDD61 pKa = 3.24 YY62 pKa = 9.35 RR63 pKa = 11.84 KK64 pKa = 10.3 GIKK67 pKa = 9.84 HH68 pKa = 5.58 EE69 pKa = 4.12
Molecular weight: 7.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.333
IPC2_protein 4.507
IPC_protein 4.329
Toseland 4.164
ProMoST 4.355
Dawson 4.291
Bjellqvist 4.546
Wikipedia 4.202
Rodwell 4.164
Grimsley 4.088
Solomon 4.279
Lehninger 4.24
Nozaki 4.418
DTASelect 4.584
Thurlkill 4.202
EMBOSS 4.215
Sillero 4.444
Patrickios 3.198
IPC_peptide 4.279
IPC2_peptide 4.431
IPC2.peptide.svr19 4.377
Protein with the highest isoelectric point:
>tr|A0A4D5ZLR4|A0A4D5ZLR4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan359 OX=2558659 GN=JavanS359_0013 PE=4 SV=1
MM1 pKa = 8.07 RR2 pKa = 11.84 YY3 pKa = 10.02 LEE5 pKa = 5.8 AGATWEE11 pKa = 4.69 DD12 pKa = 3.61 VAEE15 pKa = 4.52 KK16 pKa = 10.76 LNKK19 pKa = 5.44 TTRR22 pKa = 11.84 YY23 pKa = 8.12 INSRR27 pKa = 11.84 RR28 pKa = 11.84 KK29 pKa = 9.8 VIAKK33 pKa = 9.85 RR34 pKa = 11.84 FIEE37 pKa = 4.21 LKK39 pKa = 10.65 GYY41 pKa = 10.28
Molecular weight: 4.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.487
IPC_protein 9.663
Toseland 10.145
ProMoST 9.867
Dawson 10.335
Bjellqvist 9.984
Wikipedia 10.496
Rodwell 10.76
Grimsley 10.394
Solomon 10.379
Lehninger 10.35
Nozaki 10.101
DTASelect 9.984
Thurlkill 10.175
EMBOSS 10.54
Sillero 10.233
Patrickios 10.54
IPC_peptide 10.379
IPC2_peptide 8.565
IPC2.peptide.svr19 8.555
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
3073
41
388
153.7
17.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.556 ± 0.383
0.618 ± 0.109
6.281 ± 0.505
8.168 ± 0.663
4.068 ± 0.388
4.686 ± 0.56
1.464 ± 0.315
7.029 ± 0.331
11.129 ± 0.48
9.762 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.985 ± 0.297
5.76 ± 0.363
2.31 ± 0.347
4.361 ± 0.293
4.295 ± 0.432
6.411 ± 0.572
6.671 ± 0.548
5.272 ± 0.429
0.814 ± 0.109
4.361 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here