Terricaulis silvestris
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3942 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I6MHR6|A0A6I6MHR6_9CAUL Fatty acid desaturase OS=Terricaulis silvestris OX=2686094 GN=DSM104635_01324 PE=4 SV=1
MM1 pKa = 7.54 FIADD5 pKa = 4.93 PEE7 pKa = 4.67 LNWTNNWLGFNGYY20 pKa = 9.28 GATTLIHH27 pKa = 6.43 EE28 pKa = 5.28 LGHH31 pKa = 6.54 SIGLSHH37 pKa = 7.56 PGAYY41 pKa = 10.04 NFAPGIPLSYY51 pKa = 10.59 LGLAEE56 pKa = 4.11 YY57 pKa = 10.48 AQDD60 pKa = 3.69 SEE62 pKa = 4.89 QYY64 pKa = 11.2 SIMSYY69 pKa = 9.17 WAPAEE74 pKa = 4.04 TGAQILDD81 pKa = 3.93 FSTFLFGNAQTPMLHH96 pKa = 7.23 DD97 pKa = 4.02 IYY99 pKa = 10.79 VAQSIYY105 pKa = 10.89 GADD108 pKa = 3.43 PTTRR112 pKa = 11.84 AGDD115 pKa = 3.71 TIYY118 pKa = 11.0 GFNSTAGRR126 pKa = 11.84 DD127 pKa = 3.61 AFDD130 pKa = 4.06 FSSNAFPNVSIYY142 pKa = 10.74 DD143 pKa = 3.39 AGGNDD148 pKa = 4.19 TIDD151 pKa = 3.22 LSGFNASVFLDD162 pKa = 3.53 LHH164 pKa = 6.9 DD165 pKa = 4.58 GAFSSGAQAAPTAAVVNANRR185 pKa = 11.84 AALTALTDD193 pKa = 4.0 GAQVFAPLTQAQIDD207 pKa = 3.59 NTINIRR213 pKa = 11.84 SGNHH217 pKa = 6.44 AIFIQGDD224 pKa = 3.55 TGVAGVRR231 pKa = 11.84 ATAYY235 pKa = 10.22 DD236 pKa = 4.01 NLSIAYY242 pKa = 7.11 GTVIEE247 pKa = 4.3 NGIGGSQRR255 pKa = 11.84 DD256 pKa = 4.06 VLWGNEE262 pKa = 3.66 VANRR266 pKa = 11.84 LEE268 pKa = 4.1 GRR270 pKa = 11.84 GGNDD274 pKa = 2.93 VLNGFEE280 pKa = 4.6 GADD283 pKa = 3.67 TLVGGAGNDD292 pKa = 3.52 LFVLSVVEE300 pKa = 4.45 SGDD303 pKa = 3.44 KK304 pKa = 11.35 VMDD307 pKa = 4.04 FQTGLDD313 pKa = 4.38 DD314 pKa = 3.89 IDD316 pKa = 4.42 LRR318 pKa = 11.84 GTGIDD323 pKa = 3.49 MTWIGGAAFSGVAGQVRR340 pKa = 11.84 FASDD344 pKa = 3.52 VLSVDD349 pKa = 3.12 INGDD353 pKa = 3.24 GVADD357 pKa = 4.59 MIASVLGDD365 pKa = 4.26 DD366 pKa = 5.41 LVASDD371 pKa = 4.84 VLILL375 pKa = 4.14
Molecular weight: 39.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.541
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.757
Rodwell 3.592
Grimsley 3.439
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.202
Thurlkill 3.605
EMBOSS 3.757
Sillero 3.91
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A6I6MZJ7|A0A6I6MZJ7_9CAUL Uncharacterized protein OS=Terricaulis silvestris OX=2686094 GN=DSM104635_03403 PE=4 SV=1
MM1 pKa = 7.39 AKK3 pKa = 9.86 KK4 pKa = 10.06 VKK6 pKa = 9.96 KK7 pKa = 10.02 ARR9 pKa = 11.84 KK10 pKa = 5.98 TAKK13 pKa = 8.58 KK14 pKa = 8.52 TKK16 pKa = 9.7 ARR18 pKa = 11.84 KK19 pKa = 8.54 AAPKK23 pKa = 8.9 KK24 pKa = 7.97 TVRR27 pKa = 11.84 KK28 pKa = 8.05 AAKK31 pKa = 9.71 RR32 pKa = 11.84 KK33 pKa = 5.85 TAKK36 pKa = 8.87 KK37 pKa = 7.47 TARR40 pKa = 11.84 KK41 pKa = 7.71 TKK43 pKa = 9.11 VAKK46 pKa = 9.73 KK47 pKa = 10.19 AKK49 pKa = 8.05 TARR52 pKa = 11.84 KK53 pKa = 6.81 PAKK56 pKa = 8.68 RR57 pKa = 11.84 KK58 pKa = 9.9 KK59 pKa = 8.86 MARR62 pKa = 11.84 KK63 pKa = 6.67 TKK65 pKa = 10.3 KK66 pKa = 9.92 VVAPPVAMM74 pKa = 5.03
Molecular weight: 8.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.228
IPC_protein 12.691
Toseland 12.896
ProMoST 13.349
Dawson 12.896
Bjellqvist 12.866
Wikipedia 13.349
Rodwell 13.042
Grimsley 12.939
Solomon 13.364
Lehninger 13.276
Nozaki 12.896
DTASelect 12.866
Thurlkill 12.896
EMBOSS 13.378
Sillero 12.896
Patrickios 12.749
IPC_peptide 13.364
IPC2_peptide 12.34
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3942
0
3942
1171740
29
3256
297.2
32.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.785 ± 0.065
0.81 ± 0.012
5.765 ± 0.03
5.925 ± 0.036
3.787 ± 0.024
8.462 ± 0.044
1.882 ± 0.018
4.931 ± 0.026
2.914 ± 0.039
9.521 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.329 ± 0.018
2.69 ± 0.025
5.19 ± 0.026
3.182 ± 0.021
7.518 ± 0.044
5.232 ± 0.025
5.178 ± 0.028
7.223 ± 0.033
1.449 ± 0.017
2.228 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here