Sulfolobus islandicus rod-shaped virus 1 (SIRV-1) (Sulfolobus virus SIRV-1)

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Rudiviridae; Icerudivirus; Icerudivirus SIRV1

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q8QL32|Y84_SIRV1 Uncharacterized protein 84 OS=Sulfolobus islandicus rod-shaped virus 1 OX=157898 GN=84 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84IEE4 pKa = 5.89DD5 pKa = 3.82PFQNIFGVVRR15 pKa = 11.84IAIDD19 pKa = 3.56KK20 pKa = 10.59IKK22 pKa = 10.42EE23 pKa = 3.89LEE25 pKa = 4.01QRR27 pKa = 11.84GQIYY31 pKa = 10.19GLSRR35 pKa = 11.84FLPNSIIVMKK45 pKa = 10.23TIDD48 pKa = 3.71YY49 pKa = 9.96PLIVDD54 pKa = 4.42LYY56 pKa = 9.4IPGFTFYY63 pKa = 10.49FQFSIIQDD71 pKa = 3.58QQTKK75 pKa = 10.53QIFIDD80 pKa = 3.96DD81 pKa = 3.84FRR83 pKa = 11.84VVYY86 pKa = 7.3PQPYY90 pKa = 9.3AKK92 pKa = 10.34QINSTANFEE101 pKa = 4.26LSEE104 pKa = 4.27EE105 pKa = 4.24EE106 pKa = 4.88NEE108 pKa = 4.81DD109 pKa = 3.59EE110 pKa = 4.87

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8QL37|Y101_SIRV1 Uncharacterized protein 101 OS=Sulfolobus islandicus rod-shaped virus 1 OX=157898 GN=101 PE=4 SV=1
MM1 pKa = 7.64AKK3 pKa = 10.12GHH5 pKa = 5.64TKK7 pKa = 10.06RR8 pKa = 11.84SYY10 pKa = 7.93SQRR13 pKa = 11.84YY14 pKa = 8.74AKK16 pKa = 9.27WQAKK20 pKa = 9.13FNAFSNPTVASTILSNVAPVAQQNFQTNVPTFTAVNEE57 pKa = 4.21NVSAVLSQYY66 pKa = 11.11GITGPNRR73 pKa = 11.84AIYY76 pKa = 9.99QGFGLKK82 pKa = 9.31IARR85 pKa = 11.84ALNRR89 pKa = 11.84IGSGPALVNMINGLKK104 pKa = 9.94SYY106 pKa = 10.4YY107 pKa = 9.85ISAFNANPQVLDD119 pKa = 3.52AVVNIITGSPTGYY132 pKa = 10.61VSS134 pKa = 3.22

Molecular weight: 14.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

8526

55

1070

198.3

23.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.164 ± 0.351

0.786 ± 0.179

4.563 ± 0.299

6.697 ± 0.666

5.829 ± 0.278

4.046 ± 0.256

1.091 ± 0.151

11.049 ± 0.518

8.55 ± 0.638

9.864 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.205

7.518 ± 0.562

3.249 ± 0.346

4.539 ± 0.414

2.944 ± 0.279

6.181 ± 0.45

4.879 ± 0.282

5.172 ± 0.273

0.821 ± 0.113

6.111 ± 0.364
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski