Thermogladius calderae (strain DSM 22663 / VKM B-2946 / 1633)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Desulfurococcales; Desulfurococcaceae; Thermogladius; Thermogladius calderae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3TER3|I3TER3_THEC1 FeoA family protein OS=Thermogladius calderae (strain DSM 22663 / VKM B-2946 / 1633) OX=1184251 GN=TCELL_0827 PE=4 SV=1
MM1 pKa = 8.08SEE3 pKa = 3.76EE4 pKa = 4.06HH5 pKa = 6.23TADD8 pKa = 2.98TGAKK12 pKa = 9.17SRR14 pKa = 11.84YY15 pKa = 8.28EE16 pKa = 4.14EE17 pKa = 3.85IRR19 pKa = 11.84ALKK22 pKa = 9.68PLRR25 pKa = 11.84QGTFLGEE32 pKa = 4.13EE33 pKa = 4.46GEE35 pKa = 4.24KK36 pKa = 10.59FYY38 pKa = 11.09VAKK41 pKa = 10.65SEE43 pKa = 4.25EE44 pKa = 4.32EE45 pKa = 4.36VYY47 pKa = 10.66EE48 pKa = 4.12LSPLAYY54 pKa = 10.03YY55 pKa = 9.83IWLLCDD61 pKa = 3.71GEE63 pKa = 4.55HH64 pKa = 5.75TVEE67 pKa = 5.14DD68 pKa = 3.88VANTISSEE76 pKa = 4.11VNMPLEE82 pKa = 5.19DD83 pKa = 3.22IVEE86 pKa = 4.18PLVEE90 pKa = 4.24VLEE93 pKa = 4.51SLHH96 pKa = 6.18GAQLVVYY103 pKa = 9.68

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3TDH6|I3TDH6_THEC1 50S ribosomal protein L12 OS=Thermogladius calderae (strain DSM 22663 / VKM B-2946 / 1633) OX=1184251 GN=rpl12 PE=3 SV=1
MM1 pKa = 7.76ARR3 pKa = 11.84FKK5 pKa = 10.82PLARR9 pKa = 11.84KK10 pKa = 9.13LRR12 pKa = 11.84LAAALKK18 pKa = 10.27SNRR21 pKa = 11.84PIPIWVTLKK30 pKa = 8.86TNRR33 pKa = 11.84RR34 pKa = 11.84VRR36 pKa = 11.84RR37 pKa = 11.84AFRR40 pKa = 11.84LRR42 pKa = 11.84HH43 pKa = 4.42WRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 11.14LKK50 pKa = 10.54LL51 pKa = 3.4

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1414

0

1414

409803

37

1362

289.8

32.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.657 ± 0.058

0.686 ± 0.021

4.553 ± 0.046

7.044 ± 0.074

3.55 ± 0.034

7.706 ± 0.062

1.503 ± 0.025

5.484 ± 0.056

5.408 ± 0.069

11.222 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.99 ± 0.023

2.737 ± 0.039

4.619 ± 0.044

1.77 ± 0.032

6.659 ± 0.06

6.408 ± 0.051

4.758 ± 0.053

10.833 ± 0.06

1.13 ± 0.023

4.283 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski