Simian virus 41 (SV41)
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P35341|L_SV41 RNA-directed RNA polymerase L OS=Simian virus 41 OX=2560766 GN=L PE=3 SV=1
MM1 pKa = 7.45 SSVLKK6 pKa = 9.72 TFEE9 pKa = 4.27 RR10 pKa = 11.84 FTIQQEE16 pKa = 4.37 LQDD19 pKa = 4.5 HH20 pKa = 6.5 EE21 pKa = 4.9 EE22 pKa = 4.22 DD23 pKa = 3.74 TPVPLEE29 pKa = 4.08 TIRR32 pKa = 11.84 PLIRR36 pKa = 11.84 VFVVNSNDD44 pKa = 3.43 PALRR48 pKa = 11.84 AQLLLFNLRR57 pKa = 11.84 IIMSNTARR65 pKa = 11.84 EE66 pKa = 4.12 SHH68 pKa = 5.79 KK69 pKa = 9.86 TGALLSMFSLPAAAMGNHH87 pKa = 6.73 LKK89 pKa = 10.63 LATRR93 pKa = 11.84 SPEE96 pKa = 3.63 ASIDD100 pKa = 3.52 RR101 pKa = 11.84 VEE103 pKa = 3.87 ITGFEE108 pKa = 3.96 GRR110 pKa = 11.84 SFRR113 pKa = 11.84 VVPDD117 pKa = 3.33 ARR119 pKa = 11.84 STMSRR124 pKa = 11.84 AEE126 pKa = 3.9 VLAYY130 pKa = 9.62 EE131 pKa = 5.47 AIAEE135 pKa = 4.97 DD136 pKa = 4.79 IPDD139 pKa = 4.07 TLNHH143 pKa = 5.98 KK144 pKa = 9.06 TPFVNADD151 pKa = 3.65 VEE153 pKa = 4.24 QGDD156 pKa = 3.79 YY157 pKa = 11.56 DD158 pKa = 3.76 EE159 pKa = 5.32 TEE161 pKa = 4.42 GFLEE165 pKa = 4.19 LCYY168 pKa = 10.61 SVLMQAWIVTCKK180 pKa = 10.52 CMTAPDD186 pKa = 3.9 QPPISIEE193 pKa = 3.69 KK194 pKa = 10.45 RR195 pKa = 11.84 MAKK198 pKa = 9.66 YY199 pKa = 9.36 QQQGRR204 pKa = 11.84 INPRR208 pKa = 11.84 FILQPEE214 pKa = 4.06 ARR216 pKa = 11.84 RR217 pKa = 11.84 IIQNAIRR224 pKa = 11.84 KK225 pKa = 9.23 AMVVRR230 pKa = 11.84 HH231 pKa = 5.62 FLTYY235 pKa = 9.71 EE236 pKa = 3.74 LQMAQSKK243 pKa = 8.03 TLLANRR249 pKa = 11.84 YY250 pKa = 8.04 YY251 pKa = 11.72 AMVGDD256 pKa = 3.48 VGKK259 pKa = 10.62 YY260 pKa = 9.31 IEE262 pKa = 4.36 HH263 pKa = 6.76 SGMGGFFCTLKK274 pKa = 10.9 YY275 pKa = 10.94 GLGTRR280 pKa = 11.84 WPTLALAAFSGEE292 pKa = 4.06 LQKK295 pKa = 11.25 LKK297 pKa = 11.14 ALMLHH302 pKa = 5.45 YY303 pKa = 10.47 QSLGPMAKK311 pKa = 10.14 YY312 pKa = 8.52 MALLEE317 pKa = 4.47 SPKK320 pKa = 11.04 LMDD323 pKa = 4.61 FAPAEE328 pKa = 4.13 YY329 pKa = 9.83 PLMYY333 pKa = 9.81 SYY335 pKa = 11.85 AMGIGTVIDD344 pKa = 3.62 TNMRR348 pKa = 11.84 NYY350 pKa = 10.9 AYY352 pKa = 10.14 GRR354 pKa = 11.84 SYY356 pKa = 11.49 LNPQYY361 pKa = 10.46 FQLGVEE367 pKa = 4.63 TARR370 pKa = 11.84 KK371 pKa = 7.58 QQGAVDD377 pKa = 3.6 HH378 pKa = 6.43 RR379 pKa = 11.84 TAEE382 pKa = 4.21 DD383 pKa = 3.41 LGMSQADD390 pKa = 3.88 KK391 pKa = 11.56 VEE393 pKa = 4.1 LAATLAKK400 pKa = 9.61 LTIGQGGRR408 pKa = 11.84 GRR410 pKa = 11.84 QPLDD414 pKa = 3.43 DD415 pKa = 4.86 PFAGAAGDD423 pKa = 4.0 YY424 pKa = 10.36 QGAAAGGAQGFDD436 pKa = 3.35 YY437 pKa = 10.96 ASRR440 pKa = 11.84 RR441 pKa = 11.84 VRR443 pKa = 11.84 KK444 pKa = 9.91 YY445 pKa = 10.58 NDD447 pKa = 3.27 YY448 pKa = 11.24 EE449 pKa = 4.58 SDD451 pKa = 3.62 EE452 pKa = 4.29 EE453 pKa = 5.52 AGMDD457 pKa = 4.53 DD458 pKa = 6.04 DD459 pKa = 4.98 YY460 pKa = 11.64 EE461 pKa = 4.4 QEE463 pKa = 3.87 ARR465 pKa = 11.84 EE466 pKa = 4.02 GRR468 pKa = 11.84 GYY470 pKa = 11.05 DD471 pKa = 4.84 DD472 pKa = 5.61 DD473 pKa = 5.49 DD474 pKa = 3.76 ARR476 pKa = 11.84 QGIGGQSGFDD486 pKa = 3.11 FSVPQRR492 pKa = 11.84 APGMSDD498 pKa = 3.57 EE499 pKa = 4.47 EE500 pKa = 4.53 FQAQMTKK507 pKa = 10.57 YY508 pKa = 8.28 IQHH511 pKa = 5.49 VQQHH515 pKa = 4.66 YY516 pKa = 10.73 QEE518 pKa = 4.48 AQEE521 pKa = 4.12 GAEE524 pKa = 3.97 DD525 pKa = 3.41 GGYY528 pKa = 9.7 NQTTDD533 pKa = 3.47 DD534 pKa = 3.85 QGAGGDD540 pKa = 3.72 FDD542 pKa = 4.03 TT543 pKa = 5.6
Molecular weight: 60.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.912
IPC2_protein 4.965
IPC_protein 4.927
Toseland 4.825
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.041
Wikipedia 4.774
Rodwell 4.813
Grimsley 4.749
Solomon 4.902
Lehninger 4.863
Nozaki 5.016
DTASelect 5.181
Thurlkill 4.825
EMBOSS 4.813
Sillero 5.08
Patrickios 3.872
IPC_peptide 4.914
IPC2_peptide 5.08
IPC2.peptide.svr19 5.022
Protein with the highest isoelectric point:
>sp|P27018|NCAP_SV41 Nucleoprotein OS=Simian virus 41 OX=2560766 GN=N PE=2 SV=1
MM1 pKa = 7.07 PTISIPADD9 pKa = 3.43 PASPDD14 pKa = 3.21 QGLKK18 pKa = 10.31 PFPIQLDD25 pKa = 3.84 SKK27 pKa = 10.65 DD28 pKa = 3.71 GKK30 pKa = 10.47 SGKK33 pKa = 9.17 LVKK36 pKa = 10.24 QIRR39 pKa = 11.84 IKK41 pKa = 11.11 YY42 pKa = 7.59 LTEE45 pKa = 4.36 PNSRR49 pKa = 11.84 SPPLTFINTYY59 pKa = 9.66 GFIYY63 pKa = 10.55 ARR65 pKa = 11.84 DD66 pKa = 3.54 LSGGIMNEE74 pKa = 3.85 QSSGIQSGSVTACMMTLGPGPDD96 pKa = 2.86 IKK98 pKa = 10.98 NANRR102 pKa = 11.84 VLAALNGFYY111 pKa = 11.05 VKK113 pKa = 10.14 VRR115 pKa = 11.84 KK116 pKa = 8.02 TSSLKK121 pKa = 10.18 EE122 pKa = 3.66 EE123 pKa = 4.29 AVFEE127 pKa = 4.21 LVNVPKK133 pKa = 10.81 LLANHH138 pKa = 7.28 ALCKK142 pKa = 9.9 QGRR145 pKa = 11.84 LVCSAEE151 pKa = 4.14 KK152 pKa = 10.19 FVKK155 pKa = 10.57 NPSKK159 pKa = 10.56 MMAGQEE165 pKa = 3.98 YY166 pKa = 9.89 LYY168 pKa = 10.82 FPTFVSLTYY177 pKa = 10.62 CPSNLNYY184 pKa = 9.94 QVAKK188 pKa = 9.72 PILKK192 pKa = 9.1 IRR194 pKa = 11.84 SRR196 pKa = 11.84 FVYY199 pKa = 9.6 SIHH202 pKa = 6.04 MEE204 pKa = 3.94 IIFRR208 pKa = 11.84 LLCKK212 pKa = 9.77 PDD214 pKa = 3.57 SPLLKK219 pKa = 9.82 TYY221 pKa = 9.41 ATDD224 pKa = 3.7 PEE226 pKa = 4.57 GRR228 pKa = 11.84 GCLASVWIHH237 pKa = 5.23 VCNILKK243 pKa = 10.14 NKK245 pKa = 9.73 KK246 pKa = 8.61 IKK248 pKa = 9.95 QRR250 pKa = 11.84 GVDD253 pKa = 3.46 SYY255 pKa = 11.19 FSSKK259 pKa = 10.15 AISMQLTVSIADD271 pKa = 3.27 TWGPTVIIKK280 pKa = 10.84 ANGHH284 pKa = 5.23 IPKK287 pKa = 8.97 TAAPFFSKK295 pKa = 10.67 DD296 pKa = 2.96 GVACHH301 pKa = 6.66 PLQDD305 pKa = 3.76 VSPALTKK312 pKa = 10.62 SLWSVGCEE320 pKa = 3.39 ITKK323 pKa = 10.51 ARR325 pKa = 11.84 LILQEE330 pKa = 4.47 SNISDD335 pKa = 3.98 LLKK338 pKa = 9.76 TQDD341 pKa = 5.43 LITDD345 pKa = 3.67 QIKK348 pKa = 9.78 IKK350 pKa = 10.25 KK351 pKa = 8.01 GHH353 pKa = 4.81 SHH355 pKa = 7.66 FGRR358 pKa = 11.84 SSFNPFKK365 pKa = 10.81 KK366 pKa = 10.49 AISLPNLTQLGQDD379 pKa = 4.52 DD380 pKa = 4.07 EE381 pKa = 4.75 DD382 pKa = 3.77
Molecular weight: 42.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.88
IPC2_protein 8.756
IPC_protein 8.624
Toseland 9.487
ProMoST 9.194
Dawson 9.721
Bjellqvist 9.458
Wikipedia 9.853
Rodwell 10.16
Grimsley 9.765
Solomon 9.75
Lehninger 9.721
Nozaki 9.677
DTASelect 9.385
Thurlkill 9.589
EMBOSS 9.911
Sillero 9.692
Patrickios 5.359
IPC_peptide 9.75
IPC2_peptide 8.097
IPC2.peptide.svr19 7.958
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4943
225
2269
706.1
78.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.951 ± 1.123
1.78 ± 0.256
5.017 ± 0.448
4.673 ± 0.423
3.338 ± 0.41
5.887 ± 0.818
1.962 ± 0.324
7.04 ± 0.605
4.876 ± 0.79
10.803 ± 0.912
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.303
4.876 ± 0.473
5.341 ± 0.457
4.329 ± 0.362
4.39 ± 0.351
8.133 ± 0.568
6.777 ± 0.464
5.665 ± 0.395
1.072 ± 0.279
3.702 ± 0.501
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here