Streptomyces sp. CB03234

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6567 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5LNN2|A0A1Q5LNN2_9ACTN Transferase OS=Streptomyces sp. CB03234 OX=1703937 GN=AMK26_19100 PE=4 SV=1
MM1 pKa = 7.39LTTDD5 pKa = 4.79FVPGTPNWIDD15 pKa = 3.52LGAPDD20 pKa = 4.14TDD22 pKa = 3.55AAVAFYY28 pKa = 10.79SAVFGWTFQSAGPEE42 pKa = 3.61AGGYY46 pKa = 10.83GFFQRR51 pKa = 11.84DD52 pKa = 3.64GGTVAALGPLTEE64 pKa = 5.09EE65 pKa = 4.68GASPAWTPYY74 pKa = 10.04FHH76 pKa = 6.89TPDD79 pKa = 3.66ADD81 pKa = 3.64ATSKK85 pKa = 10.87AVEE88 pKa = 4.07QAGGTVRR95 pKa = 11.84VPPMDD100 pKa = 3.31VFTAGRR106 pKa = 11.84MAAFTDD112 pKa = 3.54PTGADD117 pKa = 3.34FAVWQPGDD125 pKa = 3.67TKK127 pKa = 11.41GLDD130 pKa = 3.19TVMEE134 pKa = 4.95PNTLCWTEE142 pKa = 5.19LYY144 pKa = 7.71TTDD147 pKa = 3.84AAAAKK152 pKa = 10.05DD153 pKa = 4.53FYY155 pKa = 11.22RR156 pKa = 11.84SVCSWQYY163 pKa = 11.29QDD165 pKa = 3.6MDD167 pKa = 3.46MGGGMVYY174 pKa = 10.33SVVSSPGGGTDD185 pKa = 4.43DD186 pKa = 5.93DD187 pKa = 4.88PGQGGIMQLQEE198 pKa = 3.97EE199 pKa = 4.37NLAAGSGSEE208 pKa = 3.49WHH210 pKa = 7.16PYY212 pKa = 10.08FGVTDD217 pKa = 4.28CDD219 pKa = 3.43ATFATATEE227 pKa = 4.5HH228 pKa = 6.84GATPLVAPIDD238 pKa = 3.91APGVGRR244 pKa = 11.84LAMLMDD250 pKa = 4.76PSGAPFALIKK260 pKa = 10.68GDD262 pKa = 3.61PTTTT266 pKa = 3.23

Molecular weight: 27.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5LHT0|A0A1Q5LHT0_9ACTN Short-chain dehydrogenase OS=Streptomyces sp. CB03234 OX=1703937 GN=AMK26_26600 PE=4 SV=1
MM1 pKa = 7.45ARR3 pKa = 11.84GAGAGLPRR11 pKa = 11.84GRR13 pKa = 11.84GHH15 pKa = 6.93RR16 pKa = 11.84AAVAGRR22 pKa = 11.84RR23 pKa = 11.84HH24 pKa = 5.98RR25 pKa = 11.84FARR28 pKa = 11.84ALSRR32 pKa = 11.84AAITASVTGFRR43 pKa = 11.84RR44 pKa = 11.84AGPVTARR51 pKa = 11.84VTASHH56 pKa = 7.73RR57 pKa = 11.84IPAHH61 pKa = 5.92RR62 pKa = 11.84APP64 pKa = 4.3

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6566

1

6567

2133522

34

5168

324.9

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.787 ± 0.048

0.805 ± 0.01

5.941 ± 0.025

5.768 ± 0.031

2.703 ± 0.017

9.49 ± 0.03

2.319 ± 0.013

3.164 ± 0.024

2.222 ± 0.022

10.341 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.77 ± 0.013

1.724 ± 0.015

6.167 ± 0.029

2.671 ± 0.018

8.114 ± 0.033

4.741 ± 0.02

6.127 ± 0.027

8.507 ± 0.029

1.511 ± 0.013

2.128 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski