Chicken associated smacovirus
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L6KWB5|A0A1L6KWB5_9VIRU Capsid protein OS=Chicken associated smacovirus OX=1932006 GN=CAP PE=4 SV=1
MM1 pKa = 7.6 NSQTYY6 pKa = 10.45 SYY8 pKa = 10.94 FLDD11 pKa = 3.68 LQTRR15 pKa = 11.84 SDD17 pKa = 3.4 RR18 pKa = 11.84 VSVVKK23 pKa = 10.23 VAAGGRR29 pKa = 11.84 EE30 pKa = 3.84 AMRR33 pKa = 11.84 RR34 pKa = 11.84 LLPFFAAYY42 pKa = 9.48 KK43 pKa = 8.7 YY44 pKa = 10.61 FKK46 pKa = 10.36 LGRR49 pKa = 11.84 VKK51 pKa = 10.87 LKK53 pKa = 9.78 FVPASTLPVDD63 pKa = 3.64 PTGLSLEE70 pKa = 4.56 AGEE73 pKa = 4.25 QTVDD77 pKa = 4.27 PRR79 pKa = 11.84 DD80 pKa = 3.35 QFNPGLVRR88 pKa = 11.84 ITNGEE93 pKa = 4.02 YY94 pKa = 9.56 WGNPQDD100 pKa = 4.81 DD101 pKa = 4.19 SQVTNMDD108 pKa = 3.26 RR109 pKa = 11.84 YY110 pKa = 8.59 YY111 pKa = 11.3 QLMLDD116 pKa = 3.72 NRR118 pKa = 11.84 WYY120 pKa = 10.83 KK121 pKa = 10.86 FNLQSGFEE129 pKa = 3.98 RR130 pKa = 11.84 HH131 pKa = 5.39 ATPLYY136 pKa = 9.72 WGIGQIRR143 pKa = 11.84 QDD145 pKa = 3.81 IFPGIAQNYY154 pKa = 7.3 PGRR157 pKa = 11.84 QAIGEE162 pKa = 4.4 SEE164 pKa = 4.45 VPQVISSKK172 pKa = 9.88 TYY174 pKa = 9.45 ISKK177 pKa = 10.37 DD178 pKa = 3.15 AQGSVKK184 pKa = 10.09 TNLIWNSDD192 pKa = 3.28 SSGWGIFQVGEE203 pKa = 4.06 RR204 pKa = 11.84 KK205 pKa = 9.67 EE206 pKa = 4.85 LGWLPTDD213 pKa = 3.45 YY214 pKa = 10.76 TLNEE218 pKa = 3.91 QLGNFPGDD226 pKa = 3.2 TGVNVPPEE234 pKa = 4.17 IEE236 pKa = 4.55 LLTIILPKK244 pKa = 10.11 AYY246 pKa = 8.48 KK247 pKa = 8.9 TIYY250 pKa = 9.1 YY251 pKa = 8.74 YY252 pKa = 10.29 RR253 pKa = 11.84 VYY255 pKa = 9.82 VTEE258 pKa = 4.23 TVHH261 pKa = 7.23 FKK263 pKa = 11.26 DD264 pKa = 3.81 PVAMNTVPMSDD275 pKa = 2.96 STQLFNVGINGYY287 pKa = 10.24 DD288 pKa = 3.43 RR289 pKa = 11.84 FWNVLAPGQASYY301 pKa = 11.44 NVTPITSTQQYY312 pKa = 10.26 NDD314 pKa = 3.19 GDD316 pKa = 3.84 GYY318 pKa = 10.93 RR319 pKa = 11.84 FRR321 pKa = 11.84 EE322 pKa = 4.79 GEE324 pKa = 3.86
Molecular weight: 36.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.28
IPC2_protein 5.525
IPC_protein 5.436
Toseland 5.448
ProMoST 5.41
Dawson 5.474
Bjellqvist 5.601
Wikipedia 5.372
Rodwell 5.385
Grimsley 5.397
Solomon 5.474
Lehninger 5.436
Nozaki 5.601
DTASelect 5.792
Thurlkill 5.474
EMBOSS 5.448
Sillero 5.677
Patrickios 3.859
IPC_peptide 5.474
IPC2_peptide 5.664
IPC2.peptide.svr19 5.776
Protein with the highest isoelectric point:
>tr|A0A1L6KWB5|A0A1L6KWB5_9VIRU Capsid protein OS=Chicken associated smacovirus OX=1932006 GN=CAP PE=4 SV=1
MM1 pKa = 7.45 NKK3 pKa = 9.26 QAYY6 pKa = 8.55 KK7 pKa = 10.82 DD8 pKa = 3.66 LGEE11 pKa = 4.36 CQSMQTYY18 pKa = 9.37 MLTIPRR24 pKa = 11.84 KK25 pKa = 6.27 VHH27 pKa = 5.94 KK28 pKa = 9.22 RR29 pKa = 11.84 TLKK32 pKa = 10.49 IMLEE36 pKa = 3.99 QNDD39 pKa = 3.56 VKK41 pKa = 10.91 KK42 pKa = 11.11 YY43 pKa = 10.31 IIAKK47 pKa = 8.7 EE48 pKa = 3.99 RR49 pKa = 11.84 GFGGYY54 pKa = 7.19 EE55 pKa = 3.27 HH56 pKa = 6.37 WQIRR60 pKa = 11.84 LKK62 pKa = 10.21 TSNKK66 pKa = 9.82 NFFIWCKK73 pKa = 10.02 INIPEE78 pKa = 4.06 AHH80 pKa = 6.39 VEE82 pKa = 4.0 EE83 pKa = 6.19 AMDD86 pKa = 3.06 TWDD89 pKa = 3.6 YY90 pKa = 10.85 EE91 pKa = 4.41 RR92 pKa = 11.84 KK93 pKa = 10.12 EE94 pKa = 4.14 GVYY97 pKa = 7.59 WTSDD101 pKa = 3.2 DD102 pKa = 3.82 TNEE105 pKa = 3.59 IRR107 pKa = 11.84 ALRR110 pKa = 11.84 FGKK113 pKa = 9.75 PNLKK117 pKa = 9.56 QKK119 pKa = 10.63 RR120 pKa = 11.84 VLKK123 pKa = 9.98 LLKK126 pKa = 10.39 YY127 pKa = 10.28 QGDD130 pKa = 3.86 RR131 pKa = 11.84 NILVWYY137 pKa = 9.81 DD138 pKa = 3.46 PVGKK142 pKa = 10.12 AGKK145 pKa = 9.25 SWIVGHH151 pKa = 6.66 LWEE154 pKa = 4.68 QGKK157 pKa = 9.8 ACYY160 pKa = 9.91 VPPTLGTPKK169 pKa = 10.45 EE170 pKa = 4.19 IIQWVHH176 pKa = 4.72 SAYY179 pKa = 10.67 DD180 pKa = 3.63 NEE182 pKa = 4.01 GLIIIDD188 pKa = 4.92 IPRR191 pKa = 11.84 SWKK194 pKa = 9.57 WNEE197 pKa = 3.71 ALYY200 pKa = 9.3 TAIEE204 pKa = 4.36 TIKK207 pKa = 10.95 DD208 pKa = 3.31 GLVYY212 pKa = 10.41 DD213 pKa = 4.16 PRR215 pKa = 11.84 YY216 pKa = 8.31 SARR219 pKa = 11.84 MKK221 pKa = 10.37 NIRR224 pKa = 11.84 GVKK227 pKa = 8.95 VLVMTNTYY235 pKa = 9.8 PRR237 pKa = 11.84 VSALSEE243 pKa = 4.12 DD244 pKa = 3.06 RR245 pKa = 11.84 WDD247 pKa = 4.21 IINEE251 pKa = 4.0 GGEE254 pKa = 4.21 APLSS258 pKa = 3.72
Molecular weight: 30.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.609
IPC2_protein 8.551
IPC_protein 8.463
Toseland 9.092
ProMoST 8.931
Dawson 9.414
Bjellqvist 9.18
Wikipedia 9.589
Rodwell 9.619
Grimsley 9.472
Solomon 9.472
Lehninger 9.428
Nozaki 9.238
DTASelect 9.136
Thurlkill 9.253
EMBOSS 9.545
Sillero 9.385
Patrickios 4.622
IPC_peptide 9.458
IPC2_peptide 7.775
IPC2.peptide.svr19 7.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
582
258
324
291.0
33.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.811 ± 0.153
0.515 ± 0.434
5.67 ± 0.163
5.67 ± 0.876
3.265 ± 1.15
7.732 ± 0.766
1.203 ± 0.493
6.529 ± 1.34
6.529 ± 2.12
7.388 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.234 ± 0.322
5.498 ± 0.308
5.326 ± 0.713
4.983 ± 1.002
5.67 ± 0.096
5.155 ± 0.858
6.014 ± 0.654
6.701 ± 0.595
3.093 ± 0.785
6.014 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here