Streptococcus satellite phage Javan560
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRJ9|A0A4D5ZRJ9_9VIRU Putative transcription regulator OS=Streptococcus satellite phage Javan560 OX=2558749 GN=JavanS560_0010 PE=4 SV=1
MM1 pKa = 7.43 TEE3 pKa = 3.92 QEE5 pKa = 4.23 FFEE8 pKa = 4.26 QAEE11 pKa = 4.44 KK12 pKa = 10.72 EE13 pKa = 4.16 LEE15 pKa = 3.98 EE16 pKa = 5.29 LNQQRR21 pKa = 11.84 ADD23 pKa = 3.95 FMAMDD28 pKa = 4.67 FKK30 pKa = 11.14 EE31 pKa = 4.79 LNNADD36 pKa = 4.08 YY37 pKa = 11.19 INFLEE42 pKa = 4.25 IGNRR46 pKa = 11.84 IIAEE50 pKa = 4.1 DD51 pKa = 3.48 VTLNVYY57 pKa = 9.26 EE58 pKa = 4.74 LYY60 pKa = 10.55 KK61 pKa = 11.05 HH62 pKa = 6.73 PDD64 pKa = 2.88 TRR66 pKa = 11.84 AKK68 pKa = 10.51 CFATIAKK75 pKa = 9.47 IAYY78 pKa = 8.24 HH79 pKa = 5.81 VNNMFQTADD88 pKa = 3.41 RR89 pKa = 11.84 MEE91 pKa = 4.33 AMIDD95 pKa = 3.39 SLEE98 pKa = 4.06 LHH100 pKa = 6.61 FQNTVKK106 pKa = 10.94 KK107 pKa = 9.38 LTLQTDD113 pKa = 3.52 SDD115 pKa = 4.1 KK116 pKa = 11.3 LAEE119 pKa = 4.53 LLLEE123 pKa = 4.33 IKK125 pKa = 10.21 KK126 pKa = 10.59 DD127 pKa = 3.9 NPNMTAEE134 pKa = 4.19 QEE136 pKa = 4.46 SQFIRR141 pKa = 11.84 DD142 pKa = 3.69 MAVSGLLAMQQ152 pKa = 4.41
Molecular weight: 17.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.621
IPC2_protein 4.558
IPC_protein 4.469
Toseland 4.317
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.266
Rodwell 4.304
Grimsley 4.228
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.66
Thurlkill 4.317
EMBOSS 4.291
Sillero 4.584
Patrickios 3.986
IPC_peptide 4.418
IPC2_peptide 4.571
IPC2.peptide.svr19 4.534
Protein with the highest isoelectric point:
>tr|A0A4D5ZT18|A0A4D5ZT18_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan560 OX=2558749 GN=JavanS560_0001 PE=4 SV=1
MM1 pKa = 7.38 IMKK4 pKa = 8.9 ITEE7 pKa = 4.09 VKK9 pKa = 10.48 KK10 pKa = 10.14 KK11 pKa = 10.37 DD12 pKa = 3.24 GSIVYY17 pKa = 9.62 RR18 pKa = 11.84 ASVYY22 pKa = 10.8 LGIDD26 pKa = 4.16 AITGKK31 pKa = 10.19 KK32 pKa = 10.01 VKK34 pKa = 10.03 TNVTGRR40 pKa = 11.84 TKK42 pKa = 10.82 KK43 pKa = 9.46 EE44 pKa = 4.12 VKK46 pKa = 10.43 NKK48 pKa = 6.58 TQQAIATFKK57 pKa = 10.34 TDD59 pKa = 2.4 GATRR63 pKa = 11.84 YY64 pKa = 9.59 QSATITSYY72 pKa = 11.51 KK73 pKa = 10.13 EE74 pKa = 4.02 LAEE77 pKa = 4.25 LWWNSYY83 pKa = 8.4 KK84 pKa = 9.73 HH85 pKa = 4.85 TVKK88 pKa = 10.64 PNTRR92 pKa = 11.84 GNIRR96 pKa = 11.84 GLLKK100 pKa = 10.74 NHH102 pKa = 6.69 VIPLFGAYY110 pKa = 9.51 KK111 pKa = 10.15 LDD113 pKa = 3.71 KK114 pKa = 10.24 LTTPLLQSIVIKK126 pKa = 10.71 LADD129 pKa = 3.5 KK130 pKa = 11.26 ANTGEE135 pKa = 4.04 AGAYY139 pKa = 8.96 LHH141 pKa = 6.56 YY142 pKa = 11.05 DD143 pKa = 4.09 KK144 pKa = 11.05 IHH146 pKa = 6.58 ALNKK150 pKa = 10.18 RR151 pKa = 11.84 ILQYY155 pKa = 10.87 GVVMQVLPYY164 pKa = 9.81 NPARR168 pKa = 11.84 EE169 pKa = 4.25 VILPRR174 pKa = 11.84 NAKK177 pKa = 9.33 KK178 pKa = 9.43 ATRR181 pKa = 11.84 KK182 pKa = 9.25 KK183 pKa = 10.03 VKK185 pKa = 10.16 HH186 pKa = 6.24 FNDD189 pKa = 3.34 EE190 pKa = 3.94 QLKK193 pKa = 10.12 QFLDD197 pKa = 3.77 YY198 pKa = 11.27 LDD200 pKa = 4.69 NLDD203 pKa = 3.61 PAKK206 pKa = 10.57 YY207 pKa = 9.45 RR208 pKa = 11.84 NIYY211 pKa = 9.89 EE212 pKa = 3.9 VTLYY216 pKa = 10.98 KK217 pKa = 10.5 FLLATGCRR225 pKa = 11.84 INEE228 pKa = 3.89 VLALHH233 pKa = 6.87 WSDD236 pKa = 4.12 IDD238 pKa = 4.14 LNNATVSITKK248 pKa = 8.91 TLNRR252 pKa = 11.84 YY253 pKa = 9.22 GSINSPKK260 pKa = 9.97 SNASIRR266 pKa = 11.84 DD267 pKa = 3.53 INIDD271 pKa = 3.67 SQTVAMMKK279 pKa = 9.85 EE280 pKa = 3.79 YY281 pKa = 10.41 RR282 pKa = 11.84 RR283 pKa = 11.84 RR284 pKa = 11.84 QIQKK288 pKa = 8.64 AWTLGRR294 pKa = 11.84 SEE296 pKa = 4.42 TVVFSDD302 pKa = 5.82 FIHH305 pKa = 7.8 DD306 pKa = 3.96 YY307 pKa = 11.31 PEE309 pKa = 5.01 DD310 pKa = 3.47 KK311 pKa = 10.71 TLGNRR316 pKa = 11.84 LTTRR320 pKa = 11.84 LKK322 pKa = 11.06 NIGLPNIGFHH332 pKa = 6.38 GFRR335 pKa = 11.84 HH336 pKa = 4.87 THH338 pKa = 6.58 ASLLLNSGIPYY349 pKa = 10.0 KK350 pKa = 10.34 EE351 pKa = 3.8 LQHH354 pKa = 6.73 RR355 pKa = 11.84 LGHH358 pKa = 5.7 SRR360 pKa = 11.84 ISMTMDD366 pKa = 3.13 IYY368 pKa = 11.65 SHH370 pKa = 6.98 LSKK373 pKa = 10.96 EE374 pKa = 4.13 NAKK377 pKa = 10.54 NAVTFYY383 pKa = 11.11 EE384 pKa = 4.32 KK385 pKa = 11.04 ALGNLL390 pKa = 3.69
Molecular weight: 44.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.111
IPC2_protein 9.414
IPC_protein 9.355
Toseland 10.072
ProMoST 9.721
Dawson 10.262
Bjellqvist 9.911
Wikipedia 10.423
Rodwell 10.789
Grimsley 10.335
Solomon 10.277
Lehninger 10.248
Nozaki 10.043
DTASelect 9.911
Thurlkill 10.116
EMBOSS 10.467
Sillero 10.175
Patrickios 10.365
IPC_peptide 10.277
IPC2_peptide 8.404
IPC2.peptide.svr19 8.426
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2054
37
390
146.7
16.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.573 ± 0.488
0.438 ± 0.167
5.891 ± 0.37
8.812 ± 1.105
3.311 ± 0.34
5.112 ± 0.486
1.801 ± 0.409
7.011 ± 0.227
9.834 ± 0.605
10.711 ± 0.54
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.775 ± 0.384
4.966 ± 0.515
2.288 ± 0.368
4.82 ± 0.567
5.063 ± 0.353
4.771 ± 0.506
6.573 ± 0.387
4.138 ± 0.297
0.925 ± 0.116
4.187 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here