Wolbachia phage WO
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9T088|A0A6B9T088_9CAUD Uncharacterized protein OS=Wolbachia phage WO OX=112596 PE=4 SV=1
MM1 pKa = 7.08 NTTFSAGEE9 pKa = 4.29 DD10 pKa = 3.72 EE11 pKa = 4.92 DD12 pKa = 4.52 SEE14 pKa = 5.4 SIISLRR20 pKa = 11.84 YY21 pKa = 8.53 GVLSVLHH28 pKa = 6.25 FPGSDD33 pKa = 3.37 DD34 pKa = 5.82 AIVEE38 pKa = 4.42 SS39 pKa = 4.35
Molecular weight: 4.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.2
IPC2_protein 3.859
IPC_protein 3.656
Toseland 3.503
ProMoST 3.872
Dawson 3.668
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.427
Solomon 3.617
Lehninger 3.567
Nozaki 3.834
DTASelect 3.961
Thurlkill 3.592
EMBOSS 3.643
Sillero 3.795
Patrickios 3.3
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A6B9SWW5|A0A6B9SWW5_9CAUD Head-tail preconnector protein OS=Wolbachia phage WO OX=112596 PE=4 SV=1
MM1 pKa = 7.36 TKK3 pKa = 10.35 AKK5 pKa = 10.33 SKK7 pKa = 11.35 LEE9 pKa = 3.83 IVNPNASGIDD19 pKa = 3.16 IGSAVHH25 pKa = 5.72 YY26 pKa = 9.89 VCVPEE31 pKa = 5.42 GRR33 pKa = 11.84 DD34 pKa = 3.23 KK35 pKa = 11.37 QRR37 pKa = 11.84 VQKK40 pKa = 10.27 FGCFTADD47 pKa = 3.23 LHH49 pKa = 7.09 NLARR53 pKa = 11.84 WLKK56 pKa = 8.19 KK57 pKa = 10.71 CKK59 pKa = 8.85 VTTVAMEE66 pKa = 4.05 STGVYY71 pKa = 9.43 WISLFQILEE80 pKa = 4.13 SYY82 pKa = 9.94 EE83 pKa = 4.35 FEE85 pKa = 4.35 VKK87 pKa = 10.25 LVNARR92 pKa = 11.84 HH93 pKa = 5.39 VKK95 pKa = 9.8 NVPGRR100 pKa = 11.84 KK101 pKa = 8.81 SDD103 pKa = 3.62 VQDD106 pKa = 4.12 CQWLQQLHH114 pKa = 6.47 SYY116 pKa = 10.58 GLLRR120 pKa = 11.84 GSFRR124 pKa = 11.84 PDD126 pKa = 2.65 NQMCVLRR133 pKa = 11.84 SYY135 pKa = 10.65 VRR137 pKa = 11.84 QRR139 pKa = 11.84 KK140 pKa = 9.06 KK141 pKa = 9.91 LTEE144 pKa = 4.02 SASTHH149 pKa = 4.52 VLRR152 pKa = 11.84 MQKK155 pKa = 10.9 ALIQMNIQLHH165 pKa = 5.37 KK166 pKa = 10.47 VISNITGVTGMKK178 pKa = 9.52 IIEE181 pKa = 4.69 AIIEE185 pKa = 4.24 GEE187 pKa = 4.09 RR188 pKa = 11.84 NPEE191 pKa = 3.48 KK192 pKa = 10.42 LAEE195 pKa = 4.11 FRR197 pKa = 11.84 SSNIKK202 pKa = 9.84 NDD204 pKa = 3.1 KK205 pKa = 9.47 ATIAKK210 pKa = 10.04 ALTGDD215 pKa = 3.51 YY216 pKa = 10.46 RR217 pKa = 11.84 EE218 pKa = 3.96 EE219 pKa = 3.85 HH220 pKa = 6.63 LFVLKK225 pKa = 10.75 QEE227 pKa = 4.25 LEE229 pKa = 4.2 LYY231 pKa = 10.56 NIYY234 pKa = 9.31 QEE236 pKa = 5.51 KK237 pKa = 10.12 IAEE240 pKa = 4.17 CDD242 pKa = 3.51 EE243 pKa = 4.79 NIEE246 pKa = 4.16 NYY248 pKa = 10.61 YY249 pKa = 9.1 KK250 pKa = 10.33 TFEE253 pKa = 4.36 TKK255 pKa = 10.08 FCGNKK260 pKa = 9.17 QCSKK264 pKa = 10.23 IKK266 pKa = 10.46 NRR268 pKa = 11.84 STKK271 pKa = 9.85 NRR273 pKa = 11.84 PNFSIHH279 pKa = 6.43 EE280 pKa = 4.08 EE281 pKa = 3.98 LHH283 pKa = 6.42 RR284 pKa = 11.84 VTGMDD289 pKa = 3.47 FTKK292 pKa = 10.67 VPGLDD297 pKa = 3.1 ALSIQTIISEE307 pKa = 4.2 TGINHH312 pKa = 6.28 NKK314 pKa = 9.03 WPTEE318 pKa = 3.65 KK319 pKa = 10.59 HH320 pKa = 4.77 FSSWLGLSPANKK332 pKa = 8.33 ITGEE336 pKa = 4.3 KK337 pKa = 10.12 VFSTRR342 pKa = 11.84 TCRR345 pKa = 11.84 VINRR349 pKa = 11.84 AANAFRR355 pKa = 11.84 MAAHH359 pKa = 6.42 CVSRR363 pKa = 11.84 SNSGIGAYY371 pKa = 9.33 CRR373 pKa = 11.84 RR374 pKa = 11.84 LKK376 pKa = 10.66 KK377 pKa = 10.49 RR378 pKa = 11.84 LGAPKK383 pKa = 10.27 AITATARR390 pKa = 11.84 KK391 pKa = 8.3 LACIFYY397 pKa = 11.17 SMLKK401 pKa = 10.13 HH402 pKa = 4.81 GQEE405 pKa = 4.22 YY406 pKa = 9.59 VEE408 pKa = 5.17 KK409 pKa = 10.96 GIDD412 pKa = 3.75 YY413 pKa = 10.75 YY414 pKa = 11.31 EE415 pKa = 4.11 KK416 pKa = 10.5 LYY418 pKa = 10.76 KK419 pKa = 10.38 EE420 pKa = 4.11 RR421 pKa = 11.84 VLKK424 pKa = 10.73 NLSKK428 pKa = 10.6 KK429 pKa = 10.48 ASEE432 pKa = 4.14 LGYY435 pKa = 11.13 VLTKK439 pKa = 10.12 KK440 pKa = 10.23 HH441 pKa = 5.58 EE442 pKa = 4.74 LII444 pKa = 4.91
Molecular weight: 50.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.113
IPC2_protein 8.96
IPC_protein 8.829
Toseland 9.692
ProMoST 9.37
Dawson 9.911
Bjellqvist 9.604
Wikipedia 10.058
Rodwell 10.35
Grimsley 9.97
Solomon 9.926
Lehninger 9.897
Nozaki 9.809
DTASelect 9.56
Thurlkill 9.765
EMBOSS 10.101
Sillero 9.853
Patrickios 7.585
IPC_peptide 9.926
IPC2_peptide 8.273
IPC2.peptide.svr19 8.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
38
0
38
8756
39
542
230.4
26.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.19 ± 0.241
1.313 ± 0.122
4.591 ± 0.334
7.709 ± 0.595
4.249 ± 0.407
6.567 ± 0.237
2.033 ± 0.158
8.771 ± 0.472
7.64 ± 0.443
9.525 ± 0.399
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.147
4.751 ± 0.336
3.243 ± 0.215
2.912 ± 0.243
5.151 ± 0.437
6.658 ± 0.314
4.922 ± 0.27
6.738 ± 0.296
1.268 ± 0.213
3.415 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here