Sinocyclocheilus rhinocerous

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi; Cypriniphysae; Cypriniformes;

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A673LUS3|A0A673LUS3_9TELE Isoform of A0A673LSP0 Protein arginine N-methyltransferase 8-B-like OS=Sinocyclocheilus rhinocerous OX=307959 GN=LOC107722452 PE=4 SV=1
MM1 pKa = 7.63CLCLIDD7 pKa = 4.89SVLLVSFSFYY17 pKa = 11.16DD18 pKa = 3.48PNLCYY23 pKa = 10.3ILDD26 pKa = 4.18ALFLLYY32 pKa = 10.63GVIITAFFVRR42 pKa = 11.84DD43 pKa = 4.26CDD45 pKa = 5.93DD46 pKa = 6.26DD47 pKa = 7.31DD48 pKa = 7.64DD49 pKa = 7.42DD50 pKa = 7.58DD51 pKa = 6.79DD52 pKa = 6.44GLFQPLQKK60 pKa = 10.7KK61 pKa = 10.47SDD63 pKa = 3.45DD64 pKa = 3.61TYY66 pKa = 11.65RR67 pKa = 11.84EE68 pKa = 4.17IEE70 pKa = 4.29TKK72 pKa = 10.43PGVSVTDD79 pKa = 3.67SLLLSVTKK87 pKa = 9.6DD88 pKa = 3.38TYY90 pKa = 11.59DD91 pKa = 3.58SLHH94 pKa = 6.03MQQIHH99 pKa = 6.26PPPRR103 pKa = 5.03

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A673JDJ6|A0A673JDJ6_9TELE Coiled-coil domain containing 125 OS=Sinocyclocheilus rhinocerous OX=307959 GN=ccdc125 PE=4 SV=1
GG1 pKa = 7.26GNTHH5 pKa = 5.85QRR7 pKa = 11.84TSITQQRR14 pKa = 11.84TSITQQRR21 pKa = 11.84TSITQQRR28 pKa = 11.84TSITHH33 pKa = 5.5ITQQRR38 pKa = 11.84TSITQQRR45 pKa = 11.84TSITQQRR52 pKa = 11.84TSITQQRR59 pKa = 11.84TSITQQRR66 pKa = 11.84TSITQQRR73 pKa = 11.84TSITQQRR80 pKa = 11.84TSITQQRR87 pKa = 11.84TSKK90 pKa = 10.98GPAA93 pKa = 2.94

Molecular weight: 10.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44739

52785

97524

58701194

7

8693

601.9

67.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.3 ± 0.006

2.314 ± 0.006

5.257 ± 0.004

6.86 ± 0.011

3.893 ± 0.005

6.021 ± 0.008

2.667 ± 0.003

4.847 ± 0.005

5.895 ± 0.007

9.714 ± 0.009

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.003

4.037 ± 0.004

5.254 ± 0.007

4.678 ± 0.008

5.444 ± 0.006

8.253 ± 0.009

5.538 ± 0.005

6.428 ± 0.007

1.168 ± 0.002

2.94 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski