Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Sporolactobacillaceae; Sporolactobacillaceae incertae sedis; [Bacillus] selenitireducens

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6XXS5|D6XXS5_BACIE Acriflavin resistance protein OS=Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10) OX=439292 GN=Bsel_2618 PE=4 SV=1
MM1 pKa = 7.44TIKK4 pKa = 10.6HH5 pKa = 6.46LLTASMALFLTACSADD21 pKa = 2.94SSTDD25 pKa = 3.33VEE27 pKa = 4.21ATEE30 pKa = 4.3DD31 pKa = 3.59QEE33 pKa = 5.42LEE35 pKa = 4.35DD36 pKa = 4.13VSIMLDD42 pKa = 3.71WYY44 pKa = 9.56PNAVHH49 pKa = 6.7SYY51 pKa = 10.1LYY53 pKa = 10.0TAMEE57 pKa = 3.45QGYY60 pKa = 9.03FEE62 pKa = 4.59EE63 pKa = 4.85EE64 pKa = 4.46GIEE67 pKa = 3.88LSIRR71 pKa = 11.84FPANPTDD78 pKa = 4.28PLSLAATGDD87 pKa = 3.24ITIGITYY94 pKa = 9.85QPDD97 pKa = 3.63VITARR102 pKa = 11.84NQGIPVVSIAAIARR116 pKa = 11.84SPLNHH121 pKa = 7.25IIYY124 pKa = 9.81TDD126 pKa = 3.74DD127 pKa = 5.21AIEE130 pKa = 4.5SPADD134 pKa = 3.5LAGKK138 pKa = 8.93QVGYY142 pKa = 9.83PGIPVNEE149 pKa = 3.99PMIDD153 pKa = 3.07TMVRR157 pKa = 11.84HH158 pKa = 6.43AGGDD162 pKa = 3.62PNEE165 pKa = 4.25VEE167 pKa = 4.76LIDD170 pKa = 3.86VGFEE174 pKa = 4.13LGSSAVSGRR183 pKa = 11.84VDD185 pKa = 3.4AVSGAYY191 pKa = 9.2INHH194 pKa = 6.16EE195 pKa = 4.39VPVLRR200 pKa = 11.84YY201 pKa = 9.27QGHH204 pKa = 5.11EE205 pKa = 3.55VHH207 pKa = 6.15YY208 pKa = 9.02FNPVDD213 pKa = 3.55YY214 pKa = 10.14GVPPFYY220 pKa = 10.49EE221 pKa = 4.03LVMVTSEE228 pKa = 3.98EE229 pKa = 4.25TLDD232 pKa = 3.57EE233 pKa = 4.31NQPLIEE239 pKa = 4.45AFWRR243 pKa = 11.84AASRR247 pKa = 11.84GFDD250 pKa = 4.79DD251 pKa = 4.41MQADD255 pKa = 4.03PDD257 pKa = 3.96GSLDD261 pKa = 3.66VLFTHH266 pKa = 7.25EE267 pKa = 4.25DD268 pKa = 3.37QEE270 pKa = 4.35NFPLIRR276 pKa = 11.84EE277 pKa = 4.23VEE279 pKa = 4.15EE280 pKa = 3.98EE281 pKa = 4.07SLGILLEE288 pKa = 4.34KK289 pKa = 8.78MTLDD293 pKa = 3.5GATFGSQYY301 pKa = 11.17ADD303 pKa = 3.38DD304 pKa = 4.03WQATIDD310 pKa = 3.48WLYY313 pKa = 10.02EE314 pKa = 3.87SGFIEE319 pKa = 4.5EE320 pKa = 5.1KK321 pKa = 10.51PDD323 pKa = 3.71TEE325 pKa = 4.66DD326 pKa = 3.47SFINLVEE333 pKa = 4.25DD334 pKa = 3.73

Molecular weight: 36.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6XSG8|D6XSG8_BACIE Pseudouridine synthase OS=Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10) OX=439292 GN=Bsel_1242 PE=3 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.18RR13 pKa = 11.84KK14 pKa = 8.17KK15 pKa = 9.24VHH17 pKa = 5.91GFRR20 pKa = 11.84QRR22 pKa = 11.84MSTSNGRR29 pKa = 11.84RR30 pKa = 11.84VLASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.73GRR40 pKa = 11.84KK41 pKa = 8.76VLSAA45 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3231

0

3231

1028036

30

3273

318.2

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.742 ± 0.048

0.599 ± 0.013

6.231 ± 0.043

7.946 ± 0.063

4.344 ± 0.036

7.257 ± 0.041

2.31 ± 0.023

6.745 ± 0.046

5.175 ± 0.039

9.328 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.135 ± 0.022

3.661 ± 0.026

3.772 ± 0.025

3.71 ± 0.025

4.973 ± 0.034

5.901 ± 0.031

5.672 ± 0.033

7.255 ± 0.035

1.033 ± 0.015

3.212 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski