Aeropyrum pernix bacilliform virus 1 (isolate -/Japan/Tanaka/2005) (APBV1)

Taxonomy: Viruses; Clavaviridae; Clavavirus; Aeropyrum pernix bacilliform virus 1

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4QF74|D4QF74_APBV1 Minor virion protein OS=Aeropyrum pernix bacilliform virus 1 (isolate -/Japan/Tanaka/2005) OX=1289471 PE=4 SV=1
MM1 pKa = 7.25NLKK4 pKa = 10.03AWAISIGVGVGLLVILPTLAGLLDD28 pKa = 3.66MAVNSIPAPSTINWSKK44 pKa = 11.31VGTIDD49 pKa = 4.07PNNNTSIQVSTTTPMLIKK67 pKa = 10.78LEE69 pKa = 4.11VTEE72 pKa = 4.33WNLVIVMKK80 pKa = 10.19QYY82 pKa = 11.43KK83 pKa = 8.92DD84 pKa = 2.79TDD86 pKa = 3.58GAIRR90 pKa = 11.84ASHH93 pKa = 6.74EE94 pKa = 4.4YY95 pKa = 9.96IDD97 pKa = 5.12DD98 pKa = 4.21PNGYY102 pKa = 7.69VTGWNWDD109 pKa = 3.64PNEE112 pKa = 3.98GVIMTSPKK120 pKa = 10.01IGFDD124 pKa = 3.26TTEE127 pKa = 3.96GSGQVEE133 pKa = 4.81WYY135 pKa = 9.45YY136 pKa = 11.4GAGALEE142 pKa = 4.39YY143 pKa = 11.1NLTTLPASVDD153 pKa = 3.44VYY155 pKa = 10.59IAEE158 pKa = 4.68GVDD161 pKa = 3.45KK162 pKa = 9.98YY163 pKa = 11.79DD164 pKa = 3.86PMAEE168 pKa = 4.15SGLGDD173 pKa = 4.03LGILARR179 pKa = 11.84VVAVFGLLAALAVAFHH195 pKa = 6.62KK196 pKa = 11.15VGGGLL201 pKa = 3.44

Molecular weight: 21.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4QF78|D4QF78_APBV1 Uncharacterized protein OS=Aeropyrum pernix bacilliform virus 1 (isolate -/Japan/Tanaka/2005) OX=1289471 PE=4 SV=1
MM1 pKa = 8.16RR2 pKa = 11.84YY3 pKa = 9.7NLLLEE8 pKa = 4.43LARR11 pKa = 11.84LKK13 pKa = 10.44PQLYY17 pKa = 10.49LMAIAPGRR25 pKa = 11.84PPWEE29 pKa = 4.18TVIKK33 pKa = 10.12AYY35 pKa = 10.79SEE37 pKa = 3.96ARR39 pKa = 11.84GLEE42 pKa = 3.99RR43 pKa = 11.84EE44 pKa = 4.29VARR47 pKa = 11.84ITLQTSYY54 pKa = 10.96RR55 pKa = 11.84SLKK58 pKa = 9.68GRR60 pKa = 11.84VVTKK64 pKa = 10.27RR65 pKa = 11.84RR66 pKa = 11.84SLGKK70 pKa = 10.36VVLTPAGSWRR80 pKa = 11.84LYY82 pKa = 10.06LIEE85 pKa = 4.44VGRR88 pKa = 11.84FDD90 pKa = 4.09

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

1553

50

201

110.9

12.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.92 ± 0.942

0.901 ± 0.413

4.765 ± 0.588

6.053 ± 1.114

3.67 ± 0.622

7.791 ± 0.882

1.417 ± 0.382

6.761 ± 0.503

4.765 ± 0.666

12.041 ± 0.642

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.347

3.284 ± 0.479

4.765 ± 0.488

2.447 ± 0.316

6.053 ± 1.023

5.087 ± 0.297

5.023 ± 0.662

9.015 ± 0.851

1.867 ± 0.328

3.863 ± 0.496
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski