Avon-Heathcote Estuary associated circular virus 23

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMK7|A0A0C5IMK7_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 23 OX=1618247 PE=4 SV=1
MM1 pKa = 7.44TARR4 pKa = 11.84NWCFTLNNPTEE15 pKa = 4.84HH16 pKa = 7.21IDD18 pKa = 3.77FKK20 pKa = 10.74SEE22 pKa = 3.05KK23 pKa = 10.21WMDD26 pKa = 3.75LKK28 pKa = 11.11LAIYY32 pKa = 9.62MMEE35 pKa = 4.45KK36 pKa = 10.78GEE38 pKa = 4.22TGTPHH43 pKa = 5.24FQGYY47 pKa = 10.36LEE49 pKa = 4.16MKK51 pKa = 9.25EE52 pKa = 4.08PQRR55 pKa = 11.84MSFFKK60 pKa = 10.86KK61 pKa = 10.25LMPRR65 pKa = 11.84AHH67 pKa = 6.21LEE69 pKa = 3.56KK70 pKa = 10.52RR71 pKa = 11.84RR72 pKa = 11.84GSKK75 pKa = 9.13QQAINYY81 pKa = 7.04VLKK84 pKa = 10.32TMDD87 pKa = 4.36PEE89 pKa = 4.5CSQWEE94 pKa = 3.91ITMTGEE100 pKa = 3.93QPIITGLPVAIHH112 pKa = 6.5CKK114 pKa = 8.9GTPHH118 pKa = 7.57DD119 pKa = 4.53LLKK122 pKa = 10.94SCEE125 pKa = 4.16PKK127 pKa = 10.45KK128 pKa = 10.6SLKK131 pKa = 10.05EE132 pKa = 3.61RR133 pKa = 11.84LEE135 pKa = 4.04DD136 pKa = 3.45VKK138 pKa = 11.16EE139 pKa = 4.45LIRR142 pKa = 11.84SGYY145 pKa = 9.76NDD147 pKa = 3.52EE148 pKa = 4.99QIMEE152 pKa = 4.06SHH154 pKa = 6.56YY155 pKa = 11.24DD156 pKa = 3.3LFVRR160 pKa = 11.84YY161 pKa = 8.97HH162 pKa = 6.46RR163 pKa = 11.84AFALTRR169 pKa = 11.84RR170 pKa = 11.84IMSSPRR176 pKa = 11.84DD177 pKa = 3.55HH178 pKa = 6.55EE179 pKa = 4.34MKK181 pKa = 10.77VIVCQGPTGTGKK193 pKa = 10.4SRR195 pKa = 11.84WAKK198 pKa = 7.72EE199 pKa = 4.28TYY201 pKa = 9.92PNAYY205 pKa = 8.08WKK207 pKa = 10.03QRR209 pKa = 11.84SQWWCGYY216 pKa = 9.12EE217 pKa = 4.11GQDD220 pKa = 4.43TIVLDD225 pKa = 4.25EE226 pKa = 4.62FYY228 pKa = 11.22GWLQYY233 pKa = 11.47DD234 pKa = 4.37LLLRR238 pKa = 11.84LCDD241 pKa = 4.16RR242 pKa = 11.84YY243 pKa = 10.26PLYY246 pKa = 11.16LEE248 pKa = 4.36TKK250 pKa = 9.84GGQVNCKK257 pKa = 9.96AKK259 pKa = 10.17TIVITTNATPEE270 pKa = 3.79SWYY273 pKa = 9.1KK274 pKa = 7.79TEE276 pKa = 5.02KK277 pKa = 10.73YY278 pKa = 9.44FLSFIRR284 pKa = 11.84RR285 pKa = 11.84VSEE288 pKa = 3.51WRR290 pKa = 11.84VFPIWGEE297 pKa = 3.81MEE299 pKa = 4.27TYY301 pKa = 10.51VLYY304 pKa = 11.03SEE306 pKa = 5.25AVSKK310 pKa = 10.46FFLNTEE316 pKa = 4.03

Molecular weight: 37.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMK7|A0A0C5IMK7_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 23 OX=1618247 PE=4 SV=1
MM1 pKa = 6.98VFARR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 8.19RR8 pKa = 11.84KK9 pKa = 7.87TYY11 pKa = 10.54AGRR14 pKa = 11.84KK15 pKa = 7.2RR16 pKa = 11.84SRR18 pKa = 11.84RR19 pKa = 11.84SFKK22 pKa = 10.16RR23 pKa = 11.84RR24 pKa = 11.84SNVKK28 pKa = 8.56RR29 pKa = 11.84RR30 pKa = 11.84MVRR33 pKa = 11.84RR34 pKa = 11.84PRR36 pKa = 11.84NIGRR40 pKa = 11.84LPGFPEE46 pKa = 4.32TYY48 pKa = 8.89KK49 pKa = 10.57TVLVYY54 pKa = 11.12NEE56 pKa = 5.2GISIDD61 pKa = 3.73AAAGGATYY69 pKa = 11.13YY70 pKa = 11.06SFNCMNLYY78 pKa = 10.29DD79 pKa = 4.5PNYY82 pKa = 9.96SGTGHH87 pKa = 6.24QPLYY91 pKa = 10.37YY92 pKa = 10.54DD93 pKa = 4.28NLTAIYY99 pKa = 10.08NRR101 pKa = 11.84WRR103 pKa = 11.84VRR105 pKa = 11.84AAYY108 pKa = 8.33ITVTILDD115 pKa = 4.13TFVSNATVDD124 pKa = 3.06AGAAYY129 pKa = 9.66RR130 pKa = 11.84AQGTGGRR137 pKa = 11.84LIVCRR142 pKa = 11.84DD143 pKa = 2.79INAADD148 pKa = 4.47NEE150 pKa = 4.67TTTNNIQEE158 pKa = 4.24EE159 pKa = 4.16RR160 pKa = 11.84SKK162 pKa = 10.92NIKK165 pKa = 8.3WRR167 pKa = 11.84YY168 pKa = 9.31FSAQTNGRR176 pKa = 11.84DD177 pKa = 3.65PKK179 pKa = 10.76LKK181 pKa = 9.93MMCIPHH187 pKa = 5.61VQLKK191 pKa = 9.08FAKK194 pKa = 10.25DD195 pKa = 3.34DD196 pKa = 3.71AQLFGSVGSGPTANCFFNIGVAGVDD221 pKa = 3.52DD222 pKa = 4.46TVNPPNVRR230 pKa = 11.84LNVRR234 pKa = 11.84ISYY237 pKa = 10.35VVDD240 pKa = 4.34FFDD243 pKa = 6.4RR244 pKa = 11.84IVDD247 pKa = 3.67QPQNN251 pKa = 3.31

Molecular weight: 28.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

567

251

316

283.5

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.82 ± 1.576

2.116 ± 0.324

4.762 ± 0.752

5.82 ± 2.37

4.409 ± 0.23

6.173 ± 0.618

1.764 ± 0.599

5.291 ± 0.177

6.702 ± 1.189

6.526 ± 1.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.732

5.291 ± 1.904

4.586 ± 0.126

3.704 ± 0.32

8.113 ± 1.637

5.115 ± 0.207

7.055 ± 0.174

5.82 ± 1.33

2.293 ± 0.926

5.467 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski