Peanut chlorotic streak virus
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q84684|Q84684_9VIRU Putative cell-to-cell transport protein OS=Peanut chlorotic streak virus OX=35593 PE=4 SV=1
MM1 pKa = 7.74 EE2 pKa = 7.07 DD3 pKa = 3.06 IFKK6 pKa = 10.16 MISEE10 pKa = 4.32 KK11 pKa = 10.56 KK12 pKa = 9.45 EE13 pKa = 3.72 KK14 pKa = 10.41 LKK16 pKa = 11.18 EE17 pKa = 4.05 EE18 pKa = 4.11 IEE20 pKa = 4.38 EE21 pKa = 4.11 KK22 pKa = 10.39 RR23 pKa = 11.84 SLVSRR28 pKa = 11.84 LQKK31 pKa = 10.43 EE32 pKa = 4.04 ISDD35 pKa = 4.02 KK36 pKa = 11.03 EE37 pKa = 4.26 KK38 pKa = 10.73 EE39 pKa = 4.03 LSALSQTEE47 pKa = 3.74 QSLGLSIKK55 pKa = 10.4 KK56 pKa = 7.79 EE57 pKa = 3.99 QPEE60 pKa = 4.04 KK61 pKa = 11.0 LVIQSSSEE69 pKa = 3.98 KK70 pKa = 10.0 YY71 pKa = 10.4 DD72 pKa = 3.35 EE73 pKa = 4.1 EE74 pKa = 4.2 EE75 pKa = 3.75 RR76 pKa = 11.84 AKK78 pKa = 10.24 RR79 pKa = 11.84 YY80 pKa = 9.16 YY81 pKa = 10.63 VIYY84 pKa = 9.98 NGPGKK89 pKa = 10.54 GIYY92 pKa = 9.82 DD93 pKa = 3.61 EE94 pKa = 4.26 WGKK97 pKa = 11.08 ASLFITGVKK106 pKa = 10.09 GIRR109 pKa = 11.84 HH110 pKa = 5.96 KK111 pKa = 11.09 KK112 pKa = 9.36 FLSKK116 pKa = 10.83 KK117 pKa = 8.13 EE118 pKa = 4.02 AQDD121 pKa = 3.9 SFNEE125 pKa = 4.12 EE126 pKa = 3.71 NKK128 pKa = 10.12 EE129 pKa = 3.86 AAKK132 pKa = 9.11 TAEE135 pKa = 3.86 VSKK138 pKa = 10.93 AYY140 pKa = 10.41 LEE142 pKa = 4.46 KK143 pKa = 10.71 LQSPKK148 pKa = 10.22 PQTSRR153 pKa = 11.84 MVNLGKK159 pKa = 10.11 IPSPRR164 pKa = 11.84 TIDD167 pKa = 4.27 HH168 pKa = 7.03 ILTKK172 pKa = 10.6 ADD174 pKa = 3.62 LQEE177 pKa = 4.26 KK178 pKa = 10.48 ARR180 pKa = 11.84 LTKK183 pKa = 10.31 KK184 pKa = 10.34 IYY186 pKa = 10.53 DD187 pKa = 4.08 DD188 pKa = 5.82 DD189 pKa = 5.0 YY190 pKa = 11.48 MFLKK194 pKa = 10.36 KK195 pKa = 10.9 YY196 pKa = 10.82 SDD198 pKa = 4.06 DD199 pKa = 4.16 DD200 pKa = 4.04 KK201 pKa = 10.96 QTSIYY206 pKa = 10.07 PVDD209 pKa = 4.23 SKK211 pKa = 11.88 SLGAKK216 pKa = 9.82 AVVLPEE222 pKa = 4.12 ADD224 pKa = 3.58 NIKK227 pKa = 10.02 TYY229 pKa = 10.23 MLYY232 pKa = 9.88 QCGFIHH238 pKa = 8.03 AIYY241 pKa = 9.91 FKK243 pKa = 10.91 SLDD246 pKa = 3.1 IFRR249 pKa = 11.84 YY250 pKa = 9.38 FPEE253 pKa = 3.85 NMKK256 pKa = 10.47 RR257 pKa = 11.84 AIQVYY262 pKa = 7.61 AQRR265 pKa = 11.84 MLKK268 pKa = 10.24 AEE270 pKa = 3.8 KK271 pKa = 10.48 GMRR274 pKa = 11.84 EE275 pKa = 3.96 GFLRR279 pKa = 11.84 IYY281 pKa = 9.06 STNPEE286 pKa = 3.89 FDD288 pKa = 3.08 EE289 pKa = 4.69 SGEE292 pKa = 4.25 VVQPAIQLCQIGGSNSNYY310 pKa = 9.34 PVMGVIEE317 pKa = 4.92 DD318 pKa = 3.66 VDD320 pKa = 3.58 EE321 pKa = 5.35 LEE323 pKa = 5.23 AFLHH327 pKa = 6.11 NYY329 pKa = 8.77 TYY331 pKa = 10.97 ILQKK335 pKa = 10.59 GISVQGRR342 pKa = 11.84 INYY345 pKa = 7.89 SAKK348 pKa = 9.65 STIIWSQQARR358 pKa = 11.84 KK359 pKa = 9.14 PSEE362 pKa = 3.55 QDD364 pKa = 2.89 IQILTEE370 pKa = 3.84 FLLRR374 pKa = 11.84 TVKK377 pKa = 10.95 NEE379 pKa = 3.79 FNFSAEE385 pKa = 4.25 VKK387 pKa = 10.59 QEE389 pKa = 3.18 ICLRR393 pKa = 11.84 LQRR396 pKa = 11.84 DD397 pKa = 3.53 FSEE400 pKa = 4.75 DD401 pKa = 3.62 HH402 pKa = 6.35 VCQLCEE408 pKa = 3.87 QSSGDD413 pKa = 3.62 LEE415 pKa = 4.81 PIKK418 pKa = 10.89 EE419 pKa = 4.12 EE420 pKa = 4.01
Molecular weight: 48.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.786
IPC2_protein 5.931
IPC_protein 5.944
Toseland 6.135
ProMoST 6.033
Dawson 6.109
Bjellqvist 6.148
Wikipedia 6.059
Rodwell 6.071
Grimsley 6.211
Solomon 6.109
Lehninger 6.084
Nozaki 6.326
DTASelect 6.491
Thurlkill 6.453
EMBOSS 6.415
Sillero 6.427
Patrickios 4.368
IPC_peptide 6.122
IPC2_peptide 6.3
IPC2.peptide.svr19 6.553
Protein with the highest isoelectric point:
>tr|Q84683|Q84683_9VIRU Inclusion body matrix protein OS=Peanut chlorotic streak virus OX=35593 PE=3 SV=1
MM1 pKa = 7.4 SSKK4 pKa = 10.39 NSSFIKK10 pKa = 10.3 VKK12 pKa = 10.72 LFNKK16 pKa = 9.87 YY17 pKa = 9.79 LYY19 pKa = 10.6 AYY21 pKa = 8.69 IDD23 pKa = 3.55 TGATICLAQAKK34 pKa = 9.36 ILPIKK39 pKa = 9.34 YY40 pKa = 7.09 WKK42 pKa = 10.7 KK43 pKa = 8.3 MIKK46 pKa = 9.22 PIKK49 pKa = 10.24 VRR51 pKa = 11.84 IANNKK56 pKa = 8.65 VIHH59 pKa = 6.15 IWYY62 pKa = 9.43 KK63 pKa = 10.83 AVDD66 pKa = 4.05 LVLLLEE72 pKa = 4.96 GKK74 pKa = 10.1 RR75 pKa = 11.84 FPLPSVYY82 pKa = 10.23 QQDD85 pKa = 3.23 AGLPLILGNNFLKK98 pKa = 10.69 LYY100 pKa = 10.68 NPFIQTLEE108 pKa = 4.37 TISLRR113 pKa = 11.84 CPQLEE118 pKa = 4.25 KK119 pKa = 10.77 QPSSLITTKK128 pKa = 10.17 IYY130 pKa = 9.9 NTFSLFGGVIVNILKK145 pKa = 8.76 QQIYY149 pKa = 9.92 IAIEE153 pKa = 4.13 DD154 pKa = 4.16 EE155 pKa = 4.4 VTQLLEE161 pKa = 4.99 AICSQNPLDD170 pKa = 3.89 PQKK173 pKa = 11.03 NRR175 pKa = 11.84 NQIIVHH181 pKa = 6.7 IDD183 pKa = 3.67 LIDD186 pKa = 3.57 PTKK189 pKa = 10.49 EE190 pKa = 3.76 VNVPNRR196 pKa = 11.84 IPYY199 pKa = 5.78 TQKK202 pKa = 10.96 DD203 pKa = 2.86 IDD205 pKa = 3.81 EE206 pKa = 4.52 FRR208 pKa = 11.84 EE209 pKa = 4.15 EE210 pKa = 3.76 TSKK213 pKa = 11.08 QIEE216 pKa = 4.26 LGILRR221 pKa = 11.84 QSKK224 pKa = 10.15 SPHH227 pKa = 5.9 SAPAFYY233 pKa = 10.93 VEE235 pKa = 3.96 NHH237 pKa = 5.76 NEE239 pKa = 3.48 IKK241 pKa = 10.17 RR242 pKa = 11.84 GKK244 pKa = 9.21 RR245 pKa = 11.84 RR246 pKa = 11.84 LVINYY251 pKa = 9.15 KK252 pKa = 8.7 MNKK255 pKa = 7.06 ATKK258 pKa = 9.82 GDD260 pKa = 3.77 AYY262 pKa = 11.35 NLDD265 pKa = 3.65 RR266 pKa = 11.84 LYY268 pKa = 10.78 LTDD271 pKa = 5.48 RR272 pKa = 11.84 EE273 pKa = 4.8 SNWFSTLDD281 pKa = 3.42 AKK283 pKa = 11.03 SGFLQLRR290 pKa = 11.84 LDD292 pKa = 4.26 EE293 pKa = 4.27 EE294 pKa = 4.85 TKK296 pKa = 10.59 PLTAFSCPPQMHH308 pKa = 6.83 LEE310 pKa = 4.16 YY311 pKa = 10.94 NVMPMGLKK319 pKa = 9.34 QAPSQFQRR327 pKa = 11.84 FMDD330 pKa = 3.77 NNLRR334 pKa = 11.84 GLEE337 pKa = 4.71 DD338 pKa = 3.16 ISLAYY343 pKa = 9.58 IDD345 pKa = 5.51 DD346 pKa = 4.58 IIVFTKK352 pKa = 9.22 GTKK355 pKa = 9.64 DD356 pKa = 3.32 YY357 pKa = 10.9 HH358 pKa = 6.46 LKK360 pKa = 9.84 QVARR364 pKa = 11.84 VLIQLGNHH372 pKa = 5.35 GVILSKK378 pKa = 10.84 EE379 pKa = 3.81 KK380 pKa = 10.98 AKK382 pKa = 10.56 IAFEE386 pKa = 4.34 EE387 pKa = 4.55 IEE389 pKa = 4.1 FLGLKK394 pKa = 9.3 ILKK397 pKa = 10.13 NGFIEE402 pKa = 4.34 PQKK405 pKa = 11.02 HH406 pKa = 5.37 LLEE409 pKa = 5.37 KK410 pKa = 10.15 IAEE413 pKa = 4.44 FPDD416 pKa = 3.22 QLQDD420 pKa = 2.92 RR421 pKa = 11.84 KK422 pKa = 10.55 QIQKK426 pKa = 10.4 FLGCLNYY433 pKa = 9.87 IGEE436 pKa = 4.24 KK437 pKa = 10.95 GFFKK441 pKa = 10.68 EE442 pKa = 3.76 LAKK445 pKa = 10.27 EE446 pKa = 4.02 RR447 pKa = 11.84 KK448 pKa = 9.07 VLQKK452 pKa = 9.88 MLSEE456 pKa = 4.03 KK457 pKa = 10.8 LPWKK461 pKa = 9.99 WNDD464 pKa = 3.19 LATLAVKK471 pKa = 10.36 RR472 pKa = 11.84 LKK474 pKa = 9.98 QVCKK478 pKa = 10.14 NLPRR482 pKa = 11.84 LYY484 pKa = 10.09 VAKK487 pKa = 10.36 PSDD490 pKa = 4.22 LLILTTDD497 pKa = 3.8 ASDD500 pKa = 3.93 TTWGAVLMAVPNAYY514 pKa = 9.9 EE515 pKa = 4.01 EE516 pKa = 4.31 FLSFVTNNRR525 pKa = 11.84 PSDD528 pKa = 3.38 LKK530 pKa = 10.34 TFLKK534 pKa = 10.46 QKK536 pKa = 9.66 EE537 pKa = 4.34 GSSRR541 pKa = 11.84 YY542 pKa = 9.41 AEE544 pKa = 3.82 KK545 pKa = 10.63 SVPEE549 pKa = 4.6 KK550 pKa = 10.64 LNSMHH555 pKa = 7.21 DD556 pKa = 3.36 KK557 pKa = 10.82 SHH559 pKa = 6.41 GEE561 pKa = 3.77 KK562 pKa = 10.49 AEE564 pKa = 4.2 KK565 pKa = 10.23 SVSRR569 pKa = 11.84 QTSQGRR575 pKa = 11.84 SSSQSQQSYY584 pKa = 9.56 IGSFMITKK592 pKa = 9.41 WSSGTFKK599 pKa = 10.45 PAEE602 pKa = 4.0 EE603 pKa = 4.68 NYY605 pKa = 9.09 TVHH608 pKa = 6.7 EE609 pKa = 4.49 KK610 pKa = 10.86 EE611 pKa = 4.29 LLAFVNALKK620 pKa = 10.66 KK621 pKa = 10.29 FQIDD625 pKa = 3.5 LRR627 pKa = 11.84 PVKK630 pKa = 10.54 FIFQTDD636 pKa = 3.66 NSWVQGFLVNKK647 pKa = 10.34 LKK649 pKa = 9.5 EE650 pKa = 4.13 TYY652 pKa = 10.3 NRR654 pKa = 11.84 GRR656 pKa = 11.84 LARR659 pKa = 11.84 WSMFIQQFDD668 pKa = 4.72 FISLHH673 pKa = 5.62 IAGKK677 pKa = 9.99 EE678 pKa = 3.73 NYY680 pKa = 9.95 LADD683 pKa = 3.61 TLTRR687 pKa = 11.84 EE688 pKa = 4.4 WKK690 pKa = 8.93 TSSRR694 pKa = 3.78
Molecular weight: 80.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.855
IPC2_protein 8.829
IPC_protein 8.668
Toseland 9.56
ProMoST 9.268
Dawson 9.794
Bjellqvist 9.458
Wikipedia 9.94
Rodwell 10.233
Grimsley 9.853
Solomon 9.809
Lehninger 9.78
Nozaki 9.604
DTASelect 9.443
Thurlkill 9.648
EMBOSS 9.984
Sillero 9.721
Patrickios 5.448
IPC_peptide 9.809
IPC2_peptide 7.907
IPC2.peptide.svr19 7.964
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1896
320
694
474.0
54.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.325 ± 0.623
1.53 ± 0.427
5.222 ± 0.421
8.228 ± 0.875
3.956 ± 0.455
4.483 ± 0.127
1.319 ± 0.222
8.07 ± 0.897
9.968 ± 0.544
9.124 ± 0.901
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.741 ± 0.139
5.063 ± 0.49
4.061 ± 0.317
6.013 ± 0.212
5.38 ± 0.635
6.909 ± 0.567
4.747 ± 0.354
4.641 ± 0.611
0.844 ± 0.262
4.378 ± 0.565
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here