Mirabilis leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077V1S0|A0A077V1S0_9GEMI Replication-associated protein OS=Mirabilis leaf curl virus OX=1431395 GN=AC1 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.42FPEE11 pKa = 4.46TVYY14 pKa = 11.2GLRR17 pKa = 11.84CMLAVKK23 pKa = 10.04YY24 pKa = 10.02CQLVEE29 pKa = 4.14NTYY32 pKa = 11.12SPDD35 pKa = 3.18TLGYY39 pKa = 10.84DD40 pKa = 4.78LIRR43 pKa = 11.84DD44 pKa = 4.64LILVIRR50 pKa = 11.84AKK52 pKa = 10.78NYY54 pKa = 8.53VQATSRR60 pKa = 11.84YY61 pKa = 7.01NHH63 pKa = 5.65FHH65 pKa = 6.27SRR67 pKa = 11.84LEE69 pKa = 4.28GTSPAQLRR77 pKa = 11.84QPICQPCCCPHH88 pKa = 6.64CPRR91 pKa = 11.84HH92 pKa = 5.92KK93 pKa = 10.19GKK95 pKa = 10.85GMGEE99 pKa = 3.88QAHH102 pKa = 6.13EE103 pKa = 4.57SEE105 pKa = 4.59AQDD108 pKa = 3.3VV109 pKa = 3.64

Molecular weight: 12.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077V302|A0A077V302_9GEMI AC4 Protein OS=Mirabilis leaf curl virus OX=1431395 GN=AC4 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFGSPYY27 pKa = 9.67VNRR30 pKa = 11.84AAAPTVRR37 pKa = 11.84VTKK40 pKa = 10.59ARR42 pKa = 11.84AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.99PRR54 pKa = 11.84MYY56 pKa = 10.12RR57 pKa = 11.84IYY59 pKa = 10.41RR60 pKa = 11.84SPDD63 pKa = 3.0VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.1SRR80 pKa = 11.84HH81 pKa = 6.13DD82 pKa = 3.26IQHH85 pKa = 6.35IGKK88 pKa = 8.97VMCVSDD94 pKa = 3.5VTRR97 pKa = 11.84GIGLTHH103 pKa = 6.67RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 10.03PVYY115 pKa = 10.54VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.56QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.65KK201 pKa = 9.58FIRR204 pKa = 11.84VNNYY208 pKa = 7.74VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.67FYY251 pKa = 11.26DD252 pKa = 3.51SVSNN256 pKa = 3.9

Molecular weight: 29.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1090

97

360

181.7

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.147 ± 0.475

2.294 ± 0.58

4.495 ± 0.537

4.495 ± 0.592

4.128 ± 0.717

4.954 ± 0.373

3.853 ± 0.631

5.138 ± 0.725

5.596 ± 0.8

7.248 ± 1.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.7

6.147 ± 0.863

6.055 ± 0.567

5.229 ± 0.63

6.33 ± 1.247

8.257 ± 1.353

5.688 ± 0.986

6.422 ± 1.415

1.376 ± 0.265

3.67 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski