Amino acid dipepetide frequency for Pseudomonas phage VW-6S

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.523AlaAla: 14.523 ± 2.82
1.225AlaCys: 1.225 ± 0.379
6.737AlaAsp: 6.737 ± 0.918
6.474AlaGlu: 6.474 ± 1.002
3.237AlaPhe: 3.237 ± 0.565
9.099AlaGly: 9.099 ± 1.225
1.837AlaHis: 1.837 ± 0.375
4.199AlaIle: 4.199 ± 0.56
6.387AlaLys: 6.387 ± 0.772
10.149AlaLeu: 10.149 ± 1.189
3.237AlaMet: 3.237 ± 0.628
3.937AlaAsn: 3.937 ± 0.633
4.112AlaPro: 4.112 ± 0.534
5.687AlaGln: 5.687 ± 1.315
7.349AlaArg: 7.349 ± 0.883
6.124AlaSer: 6.124 ± 0.817
5.774AlaThr: 5.774 ± 0.825
7.612AlaVal: 7.612 ± 1.059
1.925AlaTrp: 1.925 ± 0.364
2.45AlaTyr: 2.45 ± 0.482
0.0AlaXaa: 0.0 ± 0.0
Cys
1.137CysAla: 1.137 ± 0.334
0.087CysCys: 0.087 ± 0.079
0.437CysAsp: 0.437 ± 0.232
0.787CysGlu: 0.787 ± 0.253
0.175CysPhe: 0.175 ± 0.117
1.137CysGly: 1.137 ± 0.348
0.525CysHis: 0.525 ± 0.339
0.262CysIle: 0.262 ± 0.153
0.175CysLys: 0.175 ± 0.137
0.612CysLeu: 0.612 ± 0.255
0.175CysMet: 0.175 ± 0.143
0.525CysAsn: 0.525 ± 0.276
0.437CysPro: 0.437 ± 0.196
0.262CysGln: 0.262 ± 0.145
1.05CysArg: 1.05 ± 0.287
0.437CysSer: 0.437 ± 0.21
0.35CysThr: 0.35 ± 0.211
0.437CysVal: 0.437 ± 0.195
0.175CysTrp: 0.175 ± 0.112
0.175CysTyr: 0.175 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
6.474AspAla: 6.474 ± 1.062
0.35AspCys: 0.35 ± 0.168
2.975AspAsp: 2.975 ± 0.617
2.712AspGlu: 2.712 ± 0.516
2.012AspPhe: 2.012 ± 0.391
5.512AspGly: 5.512 ± 0.602
0.612AspHis: 0.612 ± 0.299
3.762AspIle: 3.762 ± 0.492
2.887AspLys: 2.887 ± 0.445
5.512AspLeu: 5.512 ± 0.595
1.575AspMet: 1.575 ± 0.314
2.362AspAsn: 2.362 ± 0.473
2.8AspPro: 2.8 ± 0.509
2.1AspGln: 2.1 ± 0.487
4.287AspArg: 4.287 ± 0.608
2.887AspSer: 2.887 ± 0.612
3.587AspThr: 3.587 ± 0.529
4.024AspVal: 4.024 ± 0.6
1.312AspTrp: 1.312 ± 0.408
1.575AspTyr: 1.575 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
7.174GluAla: 7.174 ± 0.689
0.7GluCys: 0.7 ± 0.254
3.062GluAsp: 3.062 ± 0.481
2.45GluGlu: 2.45 ± 0.528
1.837GluPhe: 1.837 ± 0.369
3.587GluGly: 3.587 ± 0.606
1.4GluHis: 1.4 ± 0.399
2.625GluIle: 2.625 ± 0.48
4.199GluLys: 4.199 ± 0.698
5.774GluLeu: 5.774 ± 0.676
2.187GluMet: 2.187 ± 0.446
1.925GluAsn: 1.925 ± 0.4
2.187GluPro: 2.187 ± 0.522
2.887GluGln: 2.887 ± 0.387
3.237GluArg: 3.237 ± 0.638
2.8GluSer: 2.8 ± 0.53
2.975GluThr: 2.975 ± 0.583
3.85GluVal: 3.85 ± 0.629
1.312GluTrp: 1.312 ± 0.418
1.4GluTyr: 1.4 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
2.187PheAla: 2.187 ± 0.435
0.437PheCys: 0.437 ± 0.213
2.537PheAsp: 2.537 ± 0.44
2.275PheGlu: 2.275 ± 0.429
1.312PhePhe: 1.312 ± 0.361
3.325PheGly: 3.325 ± 0.537
0.787PheHis: 0.787 ± 0.275
0.962PheIle: 0.962 ± 0.234
2.1PheLys: 2.1 ± 0.408
2.012PheLeu: 2.012 ± 0.395
0.7PheMet: 0.7 ± 0.259
1.05PheAsn: 1.05 ± 0.271
1.225PhePro: 1.225 ± 0.322
1.225PheGln: 1.225 ± 0.304
2.1PheArg: 2.1 ± 0.462
2.887PheSer: 2.887 ± 0.571
2.537PheThr: 2.537 ± 0.744
1.75PheVal: 1.75 ± 0.451
0.437PheTrp: 0.437 ± 0.222
1.4PheTyr: 1.4 ± 0.3
0.0PheXaa: 0.0 ± 0.0
Gly
8.311GlyAla: 8.311 ± 0.889
0.7GlyCys: 0.7 ± 0.257
5.512GlyAsp: 5.512 ± 0.661
4.812GlyGlu: 4.812 ± 0.873
3.237GlyPhe: 3.237 ± 0.558
6.649GlyGly: 6.649 ± 0.9
1.225GlyHis: 1.225 ± 0.321
3.937GlyIle: 3.937 ± 0.607
5.774GlyLys: 5.774 ± 0.478
7.349GlyLeu: 7.349 ± 0.753
2.1GlyMet: 2.1 ± 0.696
3.587GlyAsn: 3.587 ± 0.603
2.275GlyPro: 2.275 ± 0.415
3.762GlyGln: 3.762 ± 0.979
4.637GlyArg: 4.637 ± 0.574
3.412GlySer: 3.412 ± 0.602
4.462GlyThr: 4.462 ± 0.844
6.037GlyVal: 6.037 ± 0.71
2.275GlyTrp: 2.275 ± 0.389
3.15GlyTyr: 3.15 ± 0.554
0.0GlyXaa: 0.0 ± 0.0
His
1.487HisAla: 1.487 ± 0.501
0.175HisCys: 0.175 ± 0.15
1.225HisAsp: 1.225 ± 0.33
0.962HisGlu: 0.962 ± 0.288
0.962HisPhe: 0.962 ± 0.28
1.225HisGly: 1.225 ± 0.35
0.787HisHis: 0.787 ± 0.305
0.787HisIle: 0.787 ± 0.287
0.962HisLys: 0.962 ± 0.301
1.662HisLeu: 1.662 ± 0.514
0.525HisMet: 0.525 ± 0.238
0.437HisAsn: 0.437 ± 0.19
1.225HisPro: 1.225 ± 0.436
0.787HisGln: 0.787 ± 0.262
0.875HisArg: 0.875 ± 0.279
0.7HisSer: 0.7 ± 0.27
1.225HisThr: 1.225 ± 0.361
0.787HisVal: 0.787 ± 0.242
0.262HisTrp: 0.262 ± 0.147
0.612HisTyr: 0.612 ± 0.239
0.0HisXaa: 0.0 ± 0.0
Ile
5.249IleAla: 5.249 ± 0.466
0.262IleCys: 0.262 ± 0.142
4.374IleAsp: 4.374 ± 0.609
3.587IleGlu: 3.587 ± 0.573
0.787IlePhe: 0.787 ± 0.261
3.15IleGly: 3.15 ± 0.495
0.962IleHis: 0.962 ± 0.336
2.012IleIle: 2.012 ± 0.386
2.537IleLys: 2.537 ± 0.418
2.012IleLeu: 2.012 ± 0.338
0.612IleMet: 0.612 ± 0.264
1.837IleAsn: 1.837 ± 0.368
2.362IlePro: 2.362 ± 0.424
2.275IleGln: 2.275 ± 0.437
2.975IleArg: 2.975 ± 0.453
3.5IleSer: 3.5 ± 0.543
4.112IleThr: 4.112 ± 0.546
2.45IleVal: 2.45 ± 0.437
0.7IleTrp: 0.7 ± 0.217
1.312IleTyr: 1.312 ± 0.263
0.0IleXaa: 0.0 ± 0.0
Lys
7.437LysAla: 7.437 ± 1.059
0.087LysCys: 0.087 ± 0.084
2.45LysAsp: 2.45 ± 0.49
2.712LysGlu: 2.712 ± 0.461
2.012LysPhe: 2.012 ± 0.415
4.899LysGly: 4.899 ± 0.622
0.612LysHis: 0.612 ± 0.353
1.75LysIle: 1.75 ± 0.395
3.062LysLys: 3.062 ± 0.467
6.299LysLeu: 6.299 ± 0.703
1.312LysMet: 1.312 ± 0.403
2.362LysAsn: 2.362 ± 0.581
3.5LysPro: 3.5 ± 0.64
2.712LysGln: 2.712 ± 0.428
3.15LysArg: 3.15 ± 0.656
3.325LysSer: 3.325 ± 0.721
2.537LysThr: 2.537 ± 0.382
2.537LysVal: 2.537 ± 0.6
0.875LysTrp: 0.875 ± 0.301
0.7LysTyr: 0.7 ± 0.22
0.0LysXaa: 0.0 ± 0.0
Leu
8.661LeuAla: 8.661 ± 1.016
1.225LeuCys: 1.225 ± 0.396
5.249LeuAsp: 5.249 ± 0.528
5.249LeuGlu: 5.249 ± 0.613
2.537LeuPhe: 2.537 ± 0.629
7.787LeuGly: 7.787 ± 0.847
1.487LeuHis: 1.487 ± 0.358
3.762LeuIle: 3.762 ± 0.654
4.549LeuLys: 4.549 ± 0.741
6.737LeuLeu: 6.737 ± 0.915
2.45LeuMet: 2.45 ± 0.515
3.325LeuAsn: 3.325 ± 0.588
4.374LeuPro: 4.374 ± 0.828
3.5LeuGln: 3.5 ± 0.65
4.987LeuArg: 4.987 ± 0.676
5.774LeuSer: 5.774 ± 0.62
5.687LeuThr: 5.687 ± 0.677
5.687LeuVal: 5.687 ± 0.719
1.487LeuTrp: 1.487 ± 0.293
2.362LeuTyr: 2.362 ± 0.372
0.0LeuXaa: 0.0 ± 0.0
Met
3.675MetAla: 3.675 ± 0.663
0.175MetCys: 0.175 ± 0.114
1.225MetAsp: 1.225 ± 0.324
0.962MetGlu: 0.962 ± 0.287
0.525MetPhe: 0.525 ± 0.266
2.275MetGly: 2.275 ± 0.505
0.175MetHis: 0.175 ± 0.112
1.225MetIle: 1.225 ± 0.271
1.662MetLys: 1.662 ± 0.334
3.5MetLeu: 3.5 ± 0.6
0.7MetMet: 0.7 ± 0.225
1.05MetAsn: 1.05 ± 0.295
1.75MetPro: 1.75 ± 0.404
1.137MetGln: 1.137 ± 0.27
1.487MetArg: 1.487 ± 0.292
1.75MetSer: 1.75 ± 0.346
2.187MetThr: 2.187 ± 0.503
1.925MetVal: 1.925 ± 0.563
0.35MetTrp: 0.35 ± 0.21
0.262MetTyr: 0.262 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
4.199AsnAla: 4.199 ± 0.768
0.087AsnCys: 0.087 ± 0.086
2.012AsnAsp: 2.012 ± 0.293
1.575AsnGlu: 1.575 ± 0.42
0.787AsnPhe: 0.787 ± 0.28
3.937AsnGly: 3.937 ± 0.804
1.05AsnHis: 1.05 ± 0.408
1.225AsnIle: 1.225 ± 0.292
2.45AsnLys: 2.45 ± 0.509
3.237AsnLeu: 3.237 ± 0.414
1.312AsnMet: 1.312 ± 0.444
1.75AsnAsn: 1.75 ± 0.417
2.625AsnPro: 2.625 ± 0.551
1.487AsnGln: 1.487 ± 0.375
1.662AsnArg: 1.662 ± 0.396
2.1AsnSer: 2.1 ± 0.452
2.45AsnThr: 2.45 ± 0.505
2.012AsnVal: 2.012 ± 0.378
0.437AsnTrp: 0.437 ± 0.197
1.575AsnTyr: 1.575 ± 0.361
0.0AsnXaa: 0.0 ± 0.0
Pro
4.199ProAla: 4.199 ± 0.628
0.525ProCys: 0.525 ± 0.272
3.15ProAsp: 3.15 ± 0.521
3.062ProGlu: 3.062 ± 0.632
1.05ProPhe: 1.05 ± 0.317
3.675ProGly: 3.675 ± 0.591
0.962ProHis: 0.962 ± 0.341
2.625ProIle: 2.625 ± 0.443
2.45ProLys: 2.45 ± 0.492
2.712ProLeu: 2.712 ± 0.476
1.837ProMet: 1.837 ± 0.423
1.837ProAsn: 1.837 ± 0.394
1.662ProPro: 1.662 ± 0.405
1.312ProGln: 1.312 ± 0.27
1.837ProArg: 1.837 ± 0.431
3.675ProSer: 3.675 ± 0.623
3.412ProThr: 3.412 ± 0.532
2.712ProVal: 2.712 ± 0.528
0.525ProTrp: 0.525 ± 0.19
1.05ProTyr: 1.05 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
7.262GlnAla: 7.262 ± 0.645
0.437GlnCys: 0.437 ± 0.185
1.925GlnAsp: 1.925 ± 0.506
2.275GlnGlu: 2.275 ± 0.388
1.662GlnPhe: 1.662 ± 0.394
3.587GlnGly: 3.587 ± 0.59
0.787GlnHis: 0.787 ± 0.238
2.625GlnIle: 2.625 ± 0.392
2.187GlnLys: 2.187 ± 0.537
3.587GlnLeu: 3.587 ± 0.566
1.4GlnMet: 1.4 ± 0.354
0.962GlnAsn: 0.962 ± 0.202
1.312GlnPro: 1.312 ± 0.233
1.487GlnGln: 1.487 ± 0.402
2.537GlnArg: 2.537 ± 0.543
2.537GlnSer: 2.537 ± 0.56
2.275GlnThr: 2.275 ± 0.549
3.237GlnVal: 3.237 ± 0.509
0.7GlnTrp: 0.7 ± 0.194
0.612GlnTyr: 0.612 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
5.599ArgAla: 5.599 ± 0.687
0.35ArgCys: 0.35 ± 0.17
2.975ArgAsp: 2.975 ± 0.493
5.074ArgGlu: 5.074 ± 0.728
2.1ArgPhe: 2.1 ± 0.65
4.024ArgGly: 4.024 ± 0.669
1.4ArgHis: 1.4 ± 0.351
3.237ArgIle: 3.237 ± 0.448
2.1ArgLys: 2.1 ± 0.408
6.387ArgLeu: 6.387 ± 0.928
1.4ArgMet: 1.4 ± 0.299
1.312ArgAsn: 1.312 ± 0.401
2.45ArgPro: 2.45 ± 0.479
3.15ArgGln: 3.15 ± 0.502
2.625ArgArg: 2.625 ± 0.599
3.062ArgSer: 3.062 ± 0.58
3.062ArgThr: 3.062 ± 0.569
3.85ArgVal: 3.85 ± 0.605
1.05ArgTrp: 1.05 ± 0.395
1.312ArgTyr: 1.312 ± 0.318
0.0ArgXaa: 0.0 ± 0.0
Ser
6.999SerAla: 6.999 ± 1.112
0.962SerCys: 0.962 ± 0.292
3.675SerAsp: 3.675 ± 0.549
3.237SerGlu: 3.237 ± 0.522
2.45SerPhe: 2.45 ± 0.411
5.074SerGly: 5.074 ± 0.758
0.962SerHis: 0.962 ± 0.408
3.587SerIle: 3.587 ± 0.501
2.975SerLys: 2.975 ± 0.493
5.337SerLeu: 5.337 ± 0.764
1.837SerMet: 1.837 ± 0.34
2.1SerAsn: 2.1 ± 0.448
2.187SerPro: 2.187 ± 0.496
2.8SerGln: 2.8 ± 0.419
2.887SerArg: 2.887 ± 0.462
3.675SerSer: 3.675 ± 0.7
2.625SerThr: 2.625 ± 0.468
4.549SerVal: 4.549 ± 0.765
0.875SerTrp: 0.875 ± 0.233
1.4SerTyr: 1.4 ± 0.356
0.0SerXaa: 0.0 ± 0.0
Thr
7.612ThrAla: 7.612 ± 1.498
0.35ThrCys: 0.35 ± 0.176
3.587ThrAsp: 3.587 ± 0.521
2.537ThrGlu: 2.537 ± 0.395
2.275ThrPhe: 2.275 ± 0.517
4.899ThrGly: 4.899 ± 0.542
0.787ThrHis: 0.787 ± 0.295
3.587ThrIle: 3.587 ± 0.568
2.45ThrLys: 2.45 ± 0.539
5.599ThrLeu: 5.599 ± 0.769
1.662ThrMet: 1.662 ± 0.408
3.675ThrAsn: 3.675 ± 0.497
2.625ThrPro: 2.625 ± 0.532
2.362ThrGln: 2.362 ± 0.365
2.975ThrArg: 2.975 ± 0.587
4.199ThrSer: 4.199 ± 0.539
3.85ThrThr: 3.85 ± 0.524
3.587ThrVal: 3.587 ± 0.512
0.875ThrTrp: 0.875 ± 0.265
1.75ThrTyr: 1.75 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
6.824ValAla: 6.824 ± 0.659
0.787ValCys: 0.787 ± 0.269
4.024ValAsp: 4.024 ± 0.638
4.112ValGlu: 4.112 ± 0.557
2.8ValPhe: 2.8 ± 0.458
6.124ValGly: 6.124 ± 0.991
0.437ValHis: 0.437 ± 0.208
3.15ValIle: 3.15 ± 0.619
3.062ValLys: 3.062 ± 0.547
4.199ValLeu: 4.199 ± 0.569
2.012ValMet: 2.012 ± 0.318
2.012ValAsn: 2.012 ± 0.428
3.15ValPro: 3.15 ± 0.471
2.012ValGln: 2.012 ± 0.38
3.762ValArg: 3.762 ± 0.463
4.812ValSer: 4.812 ± 0.792
4.899ValThr: 4.899 ± 0.677
5.074ValVal: 5.074 ± 0.848
0.612ValTrp: 0.612 ± 0.274
1.4ValTyr: 1.4 ± 0.297
0.0ValXaa: 0.0 ± 0.0
Trp
1.137TrpAla: 1.137 ± 0.251
0.175TrpCys: 0.175 ± 0.122
0.612TrpAsp: 0.612 ± 0.365
0.962TrpGlu: 0.962 ± 0.329
0.7TrpPhe: 0.7 ± 0.247
1.4TrpGly: 1.4 ± 0.416
0.612TrpHis: 0.612 ± 0.258
1.05TrpIle: 1.05 ± 0.366
0.875TrpLys: 0.875 ± 0.23
1.4TrpLeu: 1.4 ± 0.277
0.35TrpMet: 0.35 ± 0.154
0.7TrpAsn: 0.7 ± 0.237
0.437TrpPro: 0.437 ± 0.156
1.137TrpGln: 1.137 ± 0.273
0.612TrpArg: 0.612 ± 0.202
1.137TrpSer: 1.137 ± 0.275
1.312TrpThr: 1.312 ± 0.382
1.4TrpVal: 1.4 ± 0.402
0.262TrpTrp: 0.262 ± 0.143
0.525TrpTyr: 0.525 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.925TyrAla: 1.925 ± 0.407
0.262TyrCys: 0.262 ± 0.137
1.575TyrAsp: 1.575 ± 0.357
1.75TyrGlu: 1.75 ± 0.425
0.787TyrPhe: 0.787 ± 0.265
1.837TyrGly: 1.837 ± 0.406
0.262TyrHis: 0.262 ± 0.137
0.7TyrIle: 0.7 ± 0.211
1.487TyrLys: 1.487 ± 0.509
2.887TyrLeu: 2.887 ± 0.477
0.525TyrMet: 0.525 ± 0.203
1.225TyrAsn: 1.225 ± 0.299
1.575TyrPro: 1.575 ± 0.352
1.312TyrGln: 1.312 ± 0.332
1.487TyrArg: 1.487 ± 0.325
1.4TyrSer: 1.4 ± 0.28
1.925TyrThr: 1.925 ± 0.453
1.75TyrVal: 1.75 ± 0.331
0.35TyrTrp: 0.35 ± 0.188
0.612TyrTyr: 0.612 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (11431 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski