Rhizoctonia solani virus 717
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9Q9X2|Q9Q9X2_9VIRU Putative RNA-dependent RNA polymerase OS=Rhizoctonia solani virus 717 OX=46618 PE=4 SV=1
MM1 pKa = 7.85 LYY3 pKa = 10.41 NFKK6 pKa = 11.1 AFISKK11 pKa = 9.74 VSHH14 pKa = 6.96 SIRR17 pKa = 11.84 DD18 pKa = 3.98 YY19 pKa = 10.39 LTEE22 pKa = 4.18 AYY24 pKa = 9.2 IRR26 pKa = 11.84 RR27 pKa = 11.84 NFEE30 pKa = 3.34 WSNFNKK36 pKa = 10.28 LHH38 pKa = 6.65 TDD40 pKa = 3.53 PSTAYY45 pKa = 10.7 YY46 pKa = 10.29 DD47 pKa = 3.27 IRR49 pKa = 11.84 DD50 pKa = 3.82 PSNVRR55 pKa = 11.84 AYY57 pKa = 10.19 HH58 pKa = 6.18 ALFGTRR64 pKa = 11.84 RR65 pKa = 11.84 QPPPKK70 pKa = 9.94 QIEE73 pKa = 4.35 LEE75 pKa = 4.2 SYY77 pKa = 9.18 MLNLIHH83 pKa = 7.49 RR84 pKa = 11.84 NAIKK88 pKa = 10.32 NRR90 pKa = 11.84 PFQFYY95 pKa = 10.26 TDD97 pKa = 4.54 LFTDD101 pKa = 3.51 TDD103 pKa = 4.42 LPTDD107 pKa = 5.33 RR108 pKa = 11.84 IPKK111 pKa = 9.72 PGIEE115 pKa = 4.04 LVNAYY120 pKa = 7.22 FHH122 pKa = 7.26 PGNVIRR128 pKa = 11.84 PNPEE132 pKa = 3.13 FSKK135 pKa = 10.91 QLTPDD140 pKa = 3.26 MEE142 pKa = 6.07 LDD144 pKa = 3.38 QFEE147 pKa = 4.95 SYY149 pKa = 10.73 IPGDD153 pKa = 3.32 IDD155 pKa = 3.73 YY156 pKa = 10.79 GPPIDD161 pKa = 5.58 NEE163 pKa = 4.41 LLTLIYY169 pKa = 10.1 RR170 pKa = 11.84 KK171 pKa = 9.85 YY172 pKa = 9.83 PQYY175 pKa = 10.98 LDD177 pKa = 5.46 PITKK181 pKa = 8.61 YY182 pKa = 10.31 CRR184 pKa = 11.84 PAGTTDD190 pKa = 2.59 ATFRR194 pKa = 11.84 DD195 pKa = 4.49 FNKK198 pKa = 10.35 EE199 pKa = 3.84 QIKK202 pKa = 9.31 TEE204 pKa = 4.22 DD205 pKa = 3.25 SDD207 pKa = 4.58 ALRR210 pKa = 11.84 LSNIMDD216 pKa = 5.9 LIHH219 pKa = 6.88 EE220 pKa = 4.76 FMNITPYY227 pKa = 10.2 MPLHH231 pKa = 6.02 FVDD234 pKa = 4.25 TFYY237 pKa = 11.5 CKK239 pKa = 10.63 LPLVTGTGYY248 pKa = 10.78 HH249 pKa = 5.8 NRR251 pKa = 11.84 HH252 pKa = 5.7 SYY254 pKa = 9.57 FRR256 pKa = 11.84 RR257 pKa = 11.84 SFAHH261 pKa = 6.2 FCHH264 pKa = 6.84 PEE266 pKa = 4.07 LYY268 pKa = 10.47 AEE270 pKa = 4.65 KK271 pKa = 8.44 PTSKK275 pKa = 10.74 GYY277 pKa = 10.29 FFNATKK283 pKa = 10.48 HH284 pKa = 4.88 EE285 pKa = 3.95 NRR287 pKa = 11.84 FLVHH291 pKa = 6.88 KK292 pKa = 9.18 IKK294 pKa = 10.8 HH295 pKa = 5.41 SGYY298 pKa = 9.39 PFDD301 pKa = 4.22 FTSDD305 pKa = 3.1 SDD307 pKa = 4.3 RR308 pKa = 11.84 NSKK311 pKa = 9.03 MADD314 pKa = 3.43 FLKK317 pKa = 10.75 SFPTMMFTRR326 pKa = 11.84 NHH328 pKa = 5.28 ISKK331 pKa = 10.37 RR332 pKa = 11.84 DD333 pKa = 3.44 GTLKK337 pKa = 10.16 VRR339 pKa = 11.84 PVYY342 pKa = 10.59 AVDD345 pKa = 4.16 EE346 pKa = 4.51 LFLDD350 pKa = 4.56 LEE352 pKa = 4.57 CMLAFPATVQARR364 pKa = 11.84 KK365 pKa = 9.47 PEE367 pKa = 4.1 CCIMYY372 pKa = 10.3 GLEE375 pKa = 4.27 TIRR378 pKa = 11.84 GSNIKK383 pKa = 10.13 LDD385 pKa = 3.71 SLAQGFISFATIDD398 pKa = 3.19 WSGYY402 pKa = 6.98 DD403 pKa = 3.9 QRR405 pKa = 11.84 LPWFIVRR412 pKa = 11.84 AFFFIYY418 pKa = 10.26 LPSLLIISHH427 pKa = 6.6 GYY429 pKa = 8.34 MPTSEE434 pKa = 4.69 YY435 pKa = 10.64 EE436 pKa = 4.06 DD437 pKa = 3.28 TSMNISDD444 pKa = 3.51 MFTRR448 pKa = 11.84 FFNLINFTATWYY460 pKa = 10.72 FNMVFLSADD469 pKa = 3.08 GFAFRR474 pKa = 11.84 RR475 pKa = 11.84 QFAGVPSGMLLTQFLDD491 pKa = 3.73 SFGNLYY497 pKa = 10.67 LIIDD501 pKa = 3.87 SLLEE505 pKa = 3.99 FGCTYY510 pKa = 11.56 DD511 pKa = 5.14 DD512 pKa = 4.17 IKK514 pKa = 11.59 SLMLFIMGDD523 pKa = 3.62 DD524 pKa = 3.55 NSIFTNWTIDD534 pKa = 3.41 KK535 pKa = 9.92 LHH537 pKa = 6.93 DD538 pKa = 4.49 FISFMEE544 pKa = 4.63 RR545 pKa = 11.84 YY546 pKa = 9.0 CLKK549 pKa = 9.56 RR550 pKa = 11.84 WNMHH554 pKa = 5.18 LSKK557 pKa = 9.17 TKK559 pKa = 10.71 SVITTLRR566 pKa = 11.84 SKK568 pKa = 10.66 IEE570 pKa = 4.02 TLSYY574 pKa = 10.17 RR575 pKa = 11.84 CNFGKK580 pKa = 9.73 PRR582 pKa = 11.84 RR583 pKa = 11.84 DD584 pKa = 3.36 VEE586 pKa = 4.11 KK587 pKa = 10.91 LIAQLVYY594 pKa = 10.03 PEE596 pKa = 5.38 HH597 pKa = 6.25 GLKK600 pKa = 9.66 PQFMSSRR607 pKa = 11.84 AIGLAYY613 pKa = 10.1 ASCAQDD619 pKa = 3.26 STFHH623 pKa = 6.11 EE624 pKa = 5.44 FCHH627 pKa = 6.49 DD628 pKa = 3.66 VYY630 pKa = 11.2 RR631 pKa = 11.84 LYY633 pKa = 11.25 LPVADD638 pKa = 5.5 LSPAAIRR645 pKa = 11.84 NTRR648 pKa = 11.84 VWILKK653 pKa = 9.15 LLEE656 pKa = 4.17 MEE658 pKa = 4.2 EE659 pKa = 4.36 TEE661 pKa = 4.83 ALIPLDD667 pKa = 4.11 HH668 pKa = 7.15 FPTMSEE674 pKa = 3.84 IQHH677 pKa = 6.31 LLSYY681 pKa = 9.88 YY682 pKa = 10.19 HH683 pKa = 6.91 GPLRR687 pKa = 11.84 PEE689 pKa = 4.17 PKK691 pKa = 8.81 WNYY694 pKa = 10.04 AHH696 pKa = 7.2 FPQDD700 pKa = 3.06 PDD702 pKa = 3.88 FRR704 pKa = 11.84 PKK706 pKa = 10.84 DD707 pKa = 3.77 YY708 pKa = 10.59 VTLLDD713 pKa = 3.52 YY714 pKa = 10.49 MEE716 pKa = 5.31 RR717 pKa = 11.84 NNISFPEE724 pKa = 4.7 LINFTVV730 pKa = 3.1
Molecular weight: 85.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.007
IPC2_protein 6.008
IPC_protein 6.097
Toseland 6.504
ProMoST 6.504
Dawson 6.427
Bjellqvist 6.415
Wikipedia 6.453
Rodwell 6.427
Grimsley 6.664
Solomon 6.427
Lehninger 6.427
Nozaki 6.707
DTASelect 6.898
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.825
Patrickios 3.834
IPC_peptide 6.453
IPC2_peptide 6.751
IPC2.peptide.svr19 6.718
Protein with the highest isoelectric point:
>tr|Q9Q9X2|Q9Q9X2_9VIRU Putative RNA-dependent RNA polymerase OS=Rhizoctonia solani virus 717 OX=46618 PE=4 SV=1
MM1 pKa = 7.95 PSPKK5 pKa = 9.44 ISLQDD10 pKa = 3.4 RR11 pKa = 11.84 LSKK14 pKa = 9.4 VTNLNPEE21 pKa = 4.12 VKK23 pKa = 9.11 FTQGDD28 pKa = 4.23 KK29 pKa = 10.96 IPDD32 pKa = 3.46 RR33 pKa = 11.84 AIDD36 pKa = 3.43 FHH38 pKa = 7.0 EE39 pKa = 4.58 VYY41 pKa = 10.31 KK42 pKa = 11.13 ANSSSKK48 pKa = 9.83 IDD50 pKa = 3.49 SANVFTIDD58 pKa = 3.43 VLPNFVPILCFVIYY72 pKa = 9.8 HH73 pKa = 6.38 ANVYY77 pKa = 8.89 STDD80 pKa = 3.54 INKK83 pKa = 9.89 HH84 pKa = 4.21 GHH86 pKa = 5.99 SKK88 pKa = 9.68 TSVPTFVSYY97 pKa = 10.97 CMSIIYY103 pKa = 9.77 GYY105 pKa = 10.83 FLLSDD110 pKa = 3.85 MYY112 pKa = 11.06 VRR114 pKa = 11.84 PSPSVFASSWKK125 pKa = 10.53 NSTFRR130 pKa = 11.84 DD131 pKa = 3.4 NFATFLASLPVPEE144 pKa = 4.98 FLLPILTSFHH154 pKa = 6.93 PFADD158 pKa = 3.88 DD159 pKa = 3.11 TRR161 pKa = 11.84 HH162 pKa = 5.18 NVIFVASAAGYY173 pKa = 9.88 RR174 pKa = 11.84 YY175 pKa = 7.9 EE176 pKa = 3.85 QHH178 pKa = 6.47 FGKK181 pKa = 10.37 IIPTLAFYY189 pKa = 10.5 NIHH192 pKa = 7.57 DD193 pKa = 4.31 IVCEE197 pKa = 4.06 QPSNAQKK204 pKa = 10.77 FDD206 pKa = 3.5 ILNALYY212 pKa = 10.67 SKK214 pKa = 10.73 AIYY217 pKa = 9.97 HH218 pKa = 5.7 ITATNFNASLANYY231 pKa = 9.28 IGYY234 pKa = 10.03 GYY236 pKa = 10.63 NNAAARR242 pKa = 11.84 TNDD245 pKa = 3.64 RR246 pKa = 11.84 GHH248 pKa = 5.75 STGKK252 pKa = 9.99 LFQVFEE258 pKa = 4.68 SLFSPVLFRR267 pKa = 11.84 DD268 pKa = 3.26 YY269 pKa = 11.04 HH270 pKa = 6.18 KK271 pKa = 10.53 RR272 pKa = 11.84 HH273 pKa = 5.83 SLASISLEE281 pKa = 3.84 PPTFATSAFNVYY293 pKa = 10.22 DD294 pKa = 3.43 WLFAATRR301 pKa = 11.84 DD302 pKa = 3.6 NLAEE306 pKa = 3.98 QKK308 pKa = 10.26 IVLSSVKK315 pKa = 10.59 SFVLDD320 pKa = 3.59 SVKK323 pKa = 10.62 CSGTLADD330 pKa = 5.28 AFTASTGAQITRR342 pKa = 11.84 HH343 pKa = 5.93 GYY345 pKa = 10.64 SLMSLPTAHH354 pKa = 6.91 GKK356 pKa = 9.22 NLIDD360 pKa = 4.69 ANGDD364 pKa = 4.01 LISSAKK370 pKa = 10.42 LSATDD375 pKa = 3.66 RR376 pKa = 11.84 QISRR380 pKa = 11.84 SARR383 pKa = 11.84 DD384 pKa = 3.16 IAIEE388 pKa = 4.01 IGFLPPIDD396 pKa = 3.91 ATPSHH401 pKa = 6.57 KK402 pKa = 10.3 QPQPQALDD410 pKa = 3.98 NKK412 pKa = 9.68 DD413 pKa = 3.44 QPIPDD418 pKa = 3.65 SHH420 pKa = 8.22 VSNASWPWNLLHH432 pKa = 6.52 STLNPPTAPKK442 pKa = 10.42 LSDD445 pKa = 3.74 VIQVPSPEE453 pKa = 4.17 TEE455 pKa = 3.9 FEE457 pKa = 4.02 IFDD460 pKa = 4.21 SDD462 pKa = 3.31 RR463 pKa = 11.84 HH464 pKa = 5.95 HH465 pKa = 6.45 YY466 pKa = 10.18 PPVRR470 pKa = 11.84 VLNFPDD476 pKa = 4.85 DD477 pKa = 4.11 STVDD481 pKa = 3.21 AHH483 pKa = 7.01 LATLTGMVIEE493 pKa = 4.61 SFEE496 pKa = 4.1 IDD498 pKa = 3.7 GSVVAHH504 pKa = 6.95 PDD506 pKa = 2.93 AHH508 pKa = 6.91 LSNGVDD514 pKa = 3.48 NTWFAVSAISLRR526 pKa = 11.84 YY527 pKa = 8.35 VYY529 pKa = 10.32 QYY531 pKa = 9.42 NTLQNGNASPIRR543 pKa = 11.84 ARR545 pKa = 11.84 SRR547 pKa = 11.84 VFRR550 pKa = 11.84 PTQGAHH556 pKa = 7.14 PIATLFVDD564 pKa = 4.24 RR565 pKa = 11.84 TQINLPRR572 pKa = 11.84 PAPYY576 pKa = 10.35 LYY578 pKa = 10.62 DD579 pKa = 3.3 QLVNNTLPGLTIVQNATWIAKK600 pKa = 8.82 VKK602 pKa = 10.66 SFLGSNVSHH611 pKa = 7.28 RR612 pKa = 11.84 LRR614 pKa = 11.84 RR615 pKa = 11.84 TEE617 pKa = 3.49 NTYY620 pKa = 10.73 PGFDD624 pKa = 4.06 DD625 pKa = 4.25 RR626 pKa = 11.84 DD627 pKa = 3.55 IYY629 pKa = 10.95 VWSPYY634 pKa = 10.46 SYY636 pKa = 10.51 TPVSPRR642 pKa = 11.84 DD643 pKa = 3.68 DD644 pKa = 4.38 ADD646 pKa = 3.02 IDD648 pKa = 3.74 YY649 pKa = 10.62 SEE651 pKa = 4.14 QVTYY655 pKa = 10.82 FLTNLRR661 pKa = 11.84 TIFGTRR667 pKa = 11.84 NRR669 pKa = 11.84 MIEE672 pKa = 4.04 VQNSLEE678 pKa = 4.04 VMPVMM683 pKa = 5.69
Molecular weight: 76.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.051
IPC2_protein 5.957
IPC_protein 6.071
Toseland 6.605
ProMoST 6.465
Dawson 6.402
Bjellqvist 6.389
Wikipedia 6.44
Rodwell 6.402
Grimsley 6.81
Solomon 6.402
Lehninger 6.402
Nozaki 6.693
DTASelect 6.883
Thurlkill 6.912
EMBOSS 6.883
Sillero 6.795
Patrickios 3.948
IPC_peptide 6.427
IPC2_peptide 6.751
IPC2.peptide.svr19 6.723
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1413
683
730
706.5
81.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.369 ± 1.205
1.062 ± 0.341
6.723 ± 0.096
3.751 ± 0.799
7.077 ± 0.874
3.539 ± 0.018
3.468 ± 0.033
6.653 ± 0.046
4.176 ± 0.474
8.846 ± 0.673
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.265 ± 0.783
5.449 ± 0.501
6.723 ± 0.218
2.76 ± 0.33
5.308 ± 0.446
8.209 ± 1.251
6.582 ± 0.11
4.883 ± 1.431
1.062 ± 0.026
5.096 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here