Nocardioides sp. LS1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4366 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A401Y118|A0A401Y118_9ACTN TetR family transcriptional regulator OS=Nocardioides sp. LS1 OX=1027620 GN=NLS1_03070 PE=4 SV=1
MM1 pKa = 7.15TLKK4 pKa = 9.87LTLDD8 pKa = 3.57LTSCTGCACCMMEE21 pKa = 5.54CPDD24 pKa = 5.34LFDD27 pKa = 5.83IDD29 pKa = 5.12DD30 pKa = 4.11EE31 pKa = 5.12SGLAVLLEE39 pKa = 4.14AHH41 pKa = 6.88PSDD44 pKa = 5.0DD45 pKa = 4.07RR46 pKa = 11.84LDD48 pKa = 3.67EE49 pKa = 4.23AEE51 pKa = 4.14RR52 pKa = 11.84AVRR55 pKa = 11.84SCPEE59 pKa = 3.57GAIVLEE65 pKa = 4.45QAA67 pKa = 4.09

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A401Y205|A0A401Y205_9ACTN Putative flavin-containing monoamine oxidase AofH OS=Nocardioides sp. LS1 OX=1027620 GN=aofH_2 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.57LIIIALAAVAVLGGGRR18 pKa = 11.84FVSHH22 pKa = 7.33RR23 pKa = 11.84LATHH27 pKa = 5.14QRR29 pKa = 11.84VVPNPLARR37 pKa = 11.84INTQVHH43 pKa = 7.25RR44 pKa = 11.84INQQ47 pKa = 3.34

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4366

0

4366

1402571

40

2529

321.2

34.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.831 ± 0.048

0.766 ± 0.01

6.536 ± 0.029

5.573 ± 0.038

2.858 ± 0.02

9.236 ± 0.032

2.407 ± 0.017

3.501 ± 0.025

2.121 ± 0.031

10.1 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.923 ± 0.016

1.89 ± 0.02

5.551 ± 0.028

2.764 ± 0.02

7.401 ± 0.04

5.365 ± 0.027

6.301 ± 0.037

9.348 ± 0.039

1.531 ± 0.017

1.994 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski