Luteimonas sp. J16
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3050 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A562BYV3|A0A562BYV3_9GAMM Outer membrane protein OS=Luteimonas sp. J16 OX=935283 GN=L599_003700000220 PE=3 SV=1
MM1 pKa = 7.73 SSRR4 pKa = 11.84 KK5 pKa = 9.14 IAIATLLSAGLLAAAPASAEE25 pKa = 3.99 VSGSVTVVSDD35 pKa = 3.18 YY36 pKa = 11.3 LFRR39 pKa = 11.84 GVTQTNEE46 pKa = 3.71 KK47 pKa = 9.77 PALQSGVTWEE57 pKa = 4.58 HH58 pKa = 6.06 EE59 pKa = 4.09 SGFYY63 pKa = 9.98 VGGWGSSISWLADD76 pKa = 2.99 SDD78 pKa = 4.54 PDD80 pKa = 3.6 VSSQVEE86 pKa = 3.77 LDD88 pKa = 3.5 GFIGYY93 pKa = 9.92 AGEE96 pKa = 4.5 FGDD99 pKa = 5.02 SGFGYY104 pKa = 10.65 DD105 pKa = 4.83 VGANYY110 pKa = 10.39 YY111 pKa = 8.59 WYY113 pKa = 9.44 PGSYY117 pKa = 8.85 PAGYY121 pKa = 9.91 NDD123 pKa = 4.62 PDD125 pKa = 3.67 TLEE128 pKa = 4.31 VYY130 pKa = 10.55 FGLSWNVLSAKK141 pKa = 10.14 YY142 pKa = 10.44 SYY144 pKa = 10.58 ATTDD148 pKa = 3.28 LFGIPDD154 pKa = 3.91 SDD156 pKa = 3.8 GSTALDD162 pKa = 4.9 LGADD166 pKa = 3.62 WEE168 pKa = 4.53 FDD170 pKa = 3.73 GGWSVGVAYY179 pKa = 10.07 GRR181 pKa = 11.84 QWVSGYY187 pKa = 10.68 GGEE190 pKa = 5.31 LDD192 pKa = 3.32 YY193 pKa = 11.24 DD194 pKa = 3.9 YY195 pKa = 11.19 WKK197 pKa = 10.83 VAAGKK202 pKa = 10.21 SFEE205 pKa = 4.17 NGFGIGLEE213 pKa = 4.02 WHH215 pKa = 6.89 DD216 pKa = 4.08 TDD218 pKa = 5.89 IDD220 pKa = 4.04 GLDD223 pKa = 3.6 DD224 pKa = 3.76 TVLVSLSKK232 pKa = 11.01 SFF234 pKa = 3.75
Molecular weight: 25.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.719
IPC_protein 3.732
Toseland 3.503
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.859
Patrickios 0.299
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A562CA02|A0A562CA02_9GAMM Acyl-coenzyme A dehydrogenase OS=Luteimonas sp. J16 OX=935283 GN=L599_000100001300 PE=3 SV=1
MM1 pKa = 7.43 AAKK4 pKa = 10.01 KK5 pKa = 9.64 AAKK8 pKa = 10.02 KK9 pKa = 10.17 KK10 pKa = 8.69 VAKK13 pKa = 10.13 KK14 pKa = 9.83 AAKK17 pKa = 10.06 KK18 pKa = 8.76 PAKK21 pKa = 9.77 KK22 pKa = 10.14 AAGKK26 pKa = 10.15 KK27 pKa = 8.04 ATKK30 pKa = 9.98 KK31 pKa = 9.05 VAKK34 pKa = 10.25 KK35 pKa = 9.99 KK36 pKa = 8.78 VAKK39 pKa = 10.41 KK40 pKa = 10.0 KK41 pKa = 8.69 VAKK44 pKa = 10.41 KK45 pKa = 10.1 KK46 pKa = 8.67 VAKK49 pKa = 10.06 KK50 pKa = 9.06 ATRR53 pKa = 11.84 KK54 pKa = 8.54 VAKK57 pKa = 10.06 KK58 pKa = 8.98 ATKK61 pKa = 8.69 KK62 pKa = 7.49 TAKK65 pKa = 10.27 RR66 pKa = 11.84 PAKK69 pKa = 10.01 KK70 pKa = 9.77 AAKK73 pKa = 9.82 KK74 pKa = 8.7 ATKK77 pKa = 9.86 KK78 pKa = 9.92 AAKK81 pKa = 9.86 KK82 pKa = 9.99 KK83 pKa = 8.76 VAKK86 pKa = 8.83 KK87 pKa = 5.61 TTRR90 pKa = 11.84 KK91 pKa = 9.26 AAKK94 pKa = 9.86 KK95 pKa = 9.96 KK96 pKa = 8.81 VAKK99 pKa = 10.09 KK100 pKa = 9.49 AAKK103 pKa = 10.1 RR104 pKa = 11.84 PAKK107 pKa = 10.15 KK108 pKa = 9.82 AAKK111 pKa = 9.54 KK112 pKa = 9.55 AAKK115 pKa = 9.91 KK116 pKa = 8.71 ATKK119 pKa = 9.99 KK120 pKa = 9.94 AAKK123 pKa = 9.63 KK124 pKa = 9.84 KK125 pKa = 10.33 AAGTKK130 pKa = 9.84 KK131 pKa = 9.87 PARR134 pKa = 11.84 KK135 pKa = 8.3 ATKK138 pKa = 9.88 KK139 pKa = 9.82 KK140 pKa = 10.24 ARR142 pKa = 11.84 KK143 pKa = 7.81 ATRR146 pKa = 11.84 KK147 pKa = 9.89 SSAQALPPVPTPLII161 pKa = 4.02
Molecular weight: 17.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 11.359
IPC_protein 12.632
Toseland 12.91
ProMoST 13.29
Dawson 12.925
Bjellqvist 12.837
Wikipedia 13.32
Rodwell 13.378
Grimsley 12.954
Solomon 13.334
Lehninger 13.247
Nozaki 12.896
DTASelect 12.837
Thurlkill 12.896
EMBOSS 13.378
Sillero 12.896
Patrickios 13.086
IPC_peptide 13.334
IPC2_peptide 12.281
IPC2.peptide.svr19 8.94
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3050
0
3050
1002375
28
2717
328.6
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.795 ± 0.062
0.837 ± 0.014
6.013 ± 0.032
5.634 ± 0.037
3.282 ± 0.025
9.09 ± 0.042
2.27 ± 0.023
3.705 ± 0.034
1.992 ± 0.034
10.783 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.086 ± 0.02
2.078 ± 0.026
5.829 ± 0.038
3.195 ± 0.024
8.836 ± 0.049
4.573 ± 0.029
4.446 ± 0.031
7.75 ± 0.036
1.589 ± 0.02
2.17 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here