Luteimonas sp. J16

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Luteimonas; unclassified Luteimonas

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A562BYV3|A0A562BYV3_9GAMM Outer membrane protein OS=Luteimonas sp. J16 OX=935283 GN=L599_003700000220 PE=3 SV=1
MM1 pKa = 7.73SSRR4 pKa = 11.84KK5 pKa = 9.14IAIATLLSAGLLAAAPASAEE25 pKa = 3.99VSGSVTVVSDD35 pKa = 3.18YY36 pKa = 11.3LFRR39 pKa = 11.84GVTQTNEE46 pKa = 3.71KK47 pKa = 9.77PALQSGVTWEE57 pKa = 4.58HH58 pKa = 6.06EE59 pKa = 4.09SGFYY63 pKa = 9.98VGGWGSSISWLADD76 pKa = 2.99SDD78 pKa = 4.54PDD80 pKa = 3.6VSSQVEE86 pKa = 3.77LDD88 pKa = 3.5GFIGYY93 pKa = 9.92AGEE96 pKa = 4.5FGDD99 pKa = 5.02SGFGYY104 pKa = 10.65DD105 pKa = 4.83VGANYY110 pKa = 10.39YY111 pKa = 8.59WYY113 pKa = 9.44PGSYY117 pKa = 8.85PAGYY121 pKa = 9.91NDD123 pKa = 4.62PDD125 pKa = 3.67TLEE128 pKa = 4.31VYY130 pKa = 10.55FGLSWNVLSAKK141 pKa = 10.14YY142 pKa = 10.44SYY144 pKa = 10.58ATTDD148 pKa = 3.28LFGIPDD154 pKa = 3.91SDD156 pKa = 3.8GSTALDD162 pKa = 4.9LGADD166 pKa = 3.62WEE168 pKa = 4.53FDD170 pKa = 3.73GGWSVGVAYY179 pKa = 10.07GRR181 pKa = 11.84QWVSGYY187 pKa = 10.68GGEE190 pKa = 5.31LDD192 pKa = 3.32YY193 pKa = 11.24DD194 pKa = 3.9YY195 pKa = 11.19WKK197 pKa = 10.83VAAGKK202 pKa = 10.21SFEE205 pKa = 4.17NGFGIGLEE213 pKa = 4.02WHH215 pKa = 6.89DD216 pKa = 4.08TDD218 pKa = 5.89IDD220 pKa = 4.04GLDD223 pKa = 3.6DD224 pKa = 3.76TVLVSLSKK232 pKa = 11.01SFF234 pKa = 3.75

Molecular weight:
25.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A562CA02|A0A562CA02_9GAMM Acyl-coenzyme A dehydrogenase OS=Luteimonas sp. J16 OX=935283 GN=L599_000100001300 PE=3 SV=1
MM1 pKa = 7.43AAKK4 pKa = 10.01KK5 pKa = 9.64AAKK8 pKa = 10.02KK9 pKa = 10.17KK10 pKa = 8.69VAKK13 pKa = 10.13KK14 pKa = 9.83AAKK17 pKa = 10.06KK18 pKa = 8.76PAKK21 pKa = 9.77KK22 pKa = 10.14AAGKK26 pKa = 10.15KK27 pKa = 8.04ATKK30 pKa = 9.98KK31 pKa = 9.05VAKK34 pKa = 10.25KK35 pKa = 9.99KK36 pKa = 8.78VAKK39 pKa = 10.41KK40 pKa = 10.0KK41 pKa = 8.69VAKK44 pKa = 10.41KK45 pKa = 10.1KK46 pKa = 8.67VAKK49 pKa = 10.06KK50 pKa = 9.06ATRR53 pKa = 11.84KK54 pKa = 8.54VAKK57 pKa = 10.06KK58 pKa = 8.98ATKK61 pKa = 8.69KK62 pKa = 7.49TAKK65 pKa = 10.27RR66 pKa = 11.84PAKK69 pKa = 10.01KK70 pKa = 9.77AAKK73 pKa = 9.82KK74 pKa = 8.7ATKK77 pKa = 9.86KK78 pKa = 9.92AAKK81 pKa = 9.86KK82 pKa = 9.99KK83 pKa = 8.76VAKK86 pKa = 8.83KK87 pKa = 5.61TTRR90 pKa = 11.84KK91 pKa = 9.26AAKK94 pKa = 9.86KK95 pKa = 9.96KK96 pKa = 8.81VAKK99 pKa = 10.09KK100 pKa = 9.49AAKK103 pKa = 10.1RR104 pKa = 11.84PAKK107 pKa = 10.15KK108 pKa = 9.82AAKK111 pKa = 9.54KK112 pKa = 9.55AAKK115 pKa = 9.91KK116 pKa = 8.71ATKK119 pKa = 9.99KK120 pKa = 9.94AAKK123 pKa = 9.63KK124 pKa = 9.84KK125 pKa = 10.33AAGTKK130 pKa = 9.84KK131 pKa = 9.87PARR134 pKa = 11.84KK135 pKa = 8.3ATKK138 pKa = 9.88KK139 pKa = 9.82KK140 pKa = 10.24ARR142 pKa = 11.84KK143 pKa = 7.81ATRR146 pKa = 11.84KK147 pKa = 9.89SSAQALPPVPTPLII161 pKa = 4.02

Molecular weight:
17.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3050

0

3050

1002375

28

2717

328.6

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.795 ± 0.062

0.837 ± 0.014

6.013 ± 0.032

5.634 ± 0.037

3.282 ± 0.025

9.09 ± 0.042

2.27 ± 0.023

3.705 ± 0.034

1.992 ± 0.034

10.783 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.02

2.078 ± 0.026

5.829 ± 0.038

3.195 ± 0.024

8.836 ± 0.049

4.573 ± 0.029

4.446 ± 0.031

7.75 ± 0.036

1.589 ± 0.02

2.17 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski