Trichomonas vaginalis virus 1 (TVV1)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q90155|Q90155_TTV1 RNA-directed RNA polymerase (Fragment) OS=Trichomonas vaginalis virus 1 OX=674953 GN=pol PE=3 SV=1
MM1 pKa = 7.19 EE2 pKa = 5.64 ASANGLSHH10 pKa = 7.93 DD11 pKa = 4.42 DD12 pKa = 4.09 NANKK16 pKa = 9.65 SQNVGPSTLPRR27 pKa = 11.84 SDD29 pKa = 3.14 KK30 pKa = 11.09 QGGEE34 pKa = 3.64 KK35 pKa = 10.34 HH36 pKa = 6.28 EE37 pKa = 4.3 NSFNSFSNDD46 pKa = 2.5 FFFNFLRR53 pKa = 11.84 MSTNTHH59 pKa = 6.17 ISDD62 pKa = 3.74 SPGVSFVAKK71 pKa = 10.42 DD72 pKa = 3.41 GTPYY76 pKa = 10.95 SSLTIPSGVGRR87 pKa = 11.84 LTHH90 pKa = 6.16 NVVASAVQLNITASNTLEE108 pKa = 3.65 VDD110 pKa = 3.38 YY111 pKa = 11.5 GFGQDD116 pKa = 3.26 VSRR119 pKa = 11.84 TTGTIPIPIFDD130 pKa = 3.77 GEE132 pKa = 4.61 KK133 pKa = 9.59 YY134 pKa = 10.29 KK135 pKa = 10.09 EE136 pKa = 4.0 TARR139 pKa = 11.84 ALAAIFSKK147 pKa = 10.62 KK148 pKa = 10.06 GMSVDD153 pKa = 3.52 VTSQTVQEE161 pKa = 4.23 TLKK164 pKa = 11.21 NSDD167 pKa = 3.33 LTIATVAAGYY177 pKa = 7.73 YY178 pKa = 7.0 TALAARR184 pKa = 11.84 HH185 pKa = 5.96 EE186 pKa = 4.28 LTKK189 pKa = 10.79 DD190 pKa = 3.28 VSEE193 pKa = 4.52 AAHH196 pKa = 6.69 TIPFVTALSDD206 pKa = 3.54 TFSAALNAQRR216 pKa = 11.84 TSHH219 pKa = 6.43 VISSCLRR226 pKa = 11.84 CPNSRR231 pKa = 11.84 NAQRR235 pKa = 11.84 DD236 pKa = 3.62 IVIGTVLWNNVFVEE250 pKa = 4.4 SLSEE254 pKa = 3.98 HH255 pKa = 5.97 NMAVPNPNDD264 pKa = 2.91 ISFFIPNKK272 pKa = 9.99 ALSSSWWCAIWLLNAFLHH290 pKa = 6.5 SFIAPTRR297 pKa = 11.84 IHH299 pKa = 6.94 IFITQGEE306 pKa = 4.94 TYY308 pKa = 10.07 HH309 pKa = 6.92 LAPFTDD315 pKa = 3.16 SDD317 pKa = 3.84 VYY319 pKa = 10.42 EE320 pKa = 4.07 AVRR323 pKa = 11.84 FLLAMSKK330 pKa = 10.16 SSRR333 pKa = 11.84 PMPEE337 pKa = 3.86 SVEE340 pKa = 4.31 SMLYY344 pKa = 10.39 AYY346 pKa = 7.32 GTQMIIQPHH355 pKa = 4.82 SLYY358 pKa = 10.63 TEE360 pKa = 4.42 GGLIRR365 pKa = 11.84 RR366 pKa = 11.84 MIFTVPHH373 pKa = 6.59 LPAHH377 pKa = 6.9 GYY379 pKa = 9.65 FVTNSEE385 pKa = 4.1 FSRR388 pKa = 11.84 YY389 pKa = 7.39 MNIAVPDD396 pKa = 4.1 DD397 pKa = 3.67 PRR399 pKa = 11.84 SAKK402 pKa = 10.69 DD403 pKa = 3.21 FVIGAGTGLLQIVLAYY419 pKa = 9.72 QAAFSCAGPIALHH432 pKa = 5.63 WHH434 pKa = 6.29 ANDD437 pKa = 5.58 AISQGMDD444 pKa = 2.8 RR445 pKa = 11.84 VASIYY450 pKa = 10.99 LEE452 pKa = 3.79 GRR454 pKa = 11.84 YY455 pKa = 7.58 FTIPMAVNVATNVAQYY471 pKa = 7.91 TTMVRR476 pKa = 11.84 ADD478 pKa = 3.19 PEE480 pKa = 4.18 YY481 pKa = 10.68 RR482 pKa = 11.84 HH483 pKa = 5.66 TLDD486 pKa = 5.29 RR487 pKa = 11.84 ILPRR491 pKa = 11.84 IFGPSTDD498 pKa = 3.46 TVFDD502 pKa = 4.96 FIEE505 pKa = 4.19 SAITSSWVSIDD516 pKa = 2.85 ARR518 pKa = 11.84 KK519 pKa = 10.06 RR520 pKa = 11.84 NGRR523 pKa = 11.84 ARR525 pKa = 11.84 KK526 pKa = 9.01 FRR528 pKa = 11.84 TAFINRR534 pKa = 11.84 FHH536 pKa = 7.21 DD537 pKa = 3.54 PEE539 pKa = 3.96 FAYY542 pKa = 10.14 MFGITGNGIEE552 pKa = 4.07 RR553 pKa = 11.84 MEE555 pKa = 4.37 GKK557 pKa = 8.6 VTSNIAQEE565 pKa = 3.93 VDD567 pKa = 3.16 YY568 pKa = 11.37 LMNGGDD574 pKa = 4.79 LRR576 pKa = 11.84 NCPILRR582 pKa = 11.84 TLKK585 pKa = 9.83 AAEE588 pKa = 4.14 RR589 pKa = 11.84 EE590 pKa = 4.18 EE591 pKa = 4.72 TVTFMCKK598 pKa = 9.82 EE599 pKa = 4.18 KK600 pKa = 10.96 VGSLYY605 pKa = 10.78 AIDD608 pKa = 3.41 GTVRR612 pKa = 11.84 VFKK615 pKa = 10.72 RR616 pKa = 11.84 FEE618 pKa = 4.46 TIDD621 pKa = 3.6 LAQLGWTSHH630 pKa = 4.96 GKK632 pKa = 9.31 VMKK635 pKa = 9.69 PYY637 pKa = 10.74 AFRR640 pKa = 11.84 APVIQGMTICSTAYY654 pKa = 8.86 TSTAIDD660 pKa = 3.78 IITTVFGPLRR670 pKa = 11.84 LRR672 pKa = 11.84 VGSLFEE678 pKa = 3.97
Molecular weight: 74.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.302
IPC2_protein 6.237
IPC_protein 6.364
Toseland 6.693
ProMoST 6.664
Dawson 6.722
Bjellqvist 6.693
Wikipedia 6.737
Rodwell 6.707
Grimsley 6.854
Solomon 6.722
Lehninger 6.722
Nozaki 6.985
DTASelect 7.161
Thurlkill 7.19
EMBOSS 7.176
Sillero 7.102
Patrickios 4.202
IPC_peptide 6.737
IPC2_peptide 6.956
IPC2.peptide.svr19 6.883
Protein with the highest isoelectric point:
>tr|Q90155|Q90155_TTV1 RNA-directed RNA polymerase (Fragment) OS=Trichomonas vaginalis virus 1 OX=674953 GN=pol PE=3 SV=1
DDD2 pKa = 4.78 FLSKKK7 pKa = 10.49 VRR9 pKa = 11.84 CGPVIPSVKKK19 pKa = 9.74 HH20 pKa = 5.5 HH21 pKa = 6.27 NINYYY26 pKa = 10.17 SILKKK31 pKa = 10.49 HH32 pKa = 6.04 GNEEE36 pKa = 3.99 YY37 pKa = 9.7 FVPGYYY43 pKa = 10.73 WVLQDDD49 pKa = 5.65 DD50 pKa = 4.48 YY51 pKa = 11.87 LNAVKKK57 pKa = 9.93 VGEEE61 pKa = 4.51 DDD63 pKa = 3.98 PPNQLPYYY71 pKa = 11.11 DD72 pKa = 4.63 DD73 pKa = 6.11 DD74 pKa = 5.37 LFTYYY79 pKa = 10.56 KKK81 pKa = 9.93 LLYYY85 pKa = 10.41 DD86 pKa = 4.23 YY87 pKa = 10.51 SHHH90 pKa = 6.81 PEEE93 pKa = 4.23 RR94 pKa = 11.84 HHH96 pKa = 5.87 KK97 pKa = 10.72 PRR99 pKa = 11.84 LLTSEEE105 pKa = 4.73 EEE107 pKa = 4.08 QLFPLKKK114 pKa = 10.3 EE115 pKa = 3.76 SAARR119 pKa = 11.84 TKKK122 pKa = 11.23 NFYYY126 pKa = 11.3 RR127 pKa = 11.84 TLWNEEE133 pKa = 3.91 TSDDD137 pKa = 3.13 KK138 pKa = 11.34 AFKKK142 pKa = 10.5 GTYYY146 pKa = 10.11 DDD148 pKa = 3.91 VAGLLMWQQCALMWSLPKKK167 pKa = 10.65 IINKKK172 pKa = 9.51 ISGVCDDD179 pKa = 3.43 LTEEE183 pKa = 4.38 KK184 pKa = 10.97 SLTLLKKK191 pKa = 10.49 ISDDD195 pKa = 3.64 LKKK198 pKa = 10.38 LGLAYYY204 pKa = 10.64 PIFRR208 pKa = 11.84 LFIEEE213 pKa = 4.84 PTLLGRR219 pKa = 11.84 GAIPGDDD226 pKa = 3.15 ALDDD230 pKa = 3.59 KKK232 pKa = 10.91 HH233 pKa = 6.52 LTYYY237 pKa = 10.29 PLMTVDDD244 pKa = 3.57 PKKK247 pKa = 9.7 QLHHH251 pKa = 5.85 DD252 pKa = 4.82 IYYY255 pKa = 10.84 LLSRR259 pKa = 11.84 NYYY262 pKa = 9.41 NTKKK266 pKa = 9.54 SSFEEE271 pKa = 3.66 HH272 pKa = 6.5 HH273 pKa = 6.74 EEE275 pKa = 4.18 EE276 pKa = 4.43 LLWSRR281 pKa = 11.84 SGSHHH286 pKa = 6.32 YY287 pKa = 11.13 YY288 pKa = 9.85 DDD290 pKa = 3.61 EE291 pKa = 4.81 IDDD294 pKa = 3.8 LLPPQPTRR302 pKa = 11.84 KKK304 pKa = 9.37 EE305 pKa = 3.75 LDDD308 pKa = 3.72 VTIDDD313 pKa = 4.02 YY314 pKa = 10.63 KKK316 pKa = 9.99 CKKK319 pKa = 7.74 QVFIRR324 pKa = 11.84 QSRR327 pKa = 11.84 KKK329 pKa = 9.69 EEE331 pKa = 4.08 HH332 pKa = 5.88 KKK334 pKa = 9.68 EE335 pKa = 3.72 FIYYY339 pKa = 10.13 CDDD342 pKa = 3.73 ISYYY346 pKa = 9.69 YYY348 pKa = 10.58 DDD350 pKa = 4.24 VLKKK354 pKa = 10.68 FEEE357 pKa = 5.38 GWQDDD362 pKa = 2.84 EEE364 pKa = 5.9 ILSPGDDD371 pKa = 3.6 YY372 pKa = 10.61 SEEE375 pKa = 3.95 LHHH378 pKa = 6.73 KKK380 pKa = 9.77 SGYYY384 pKa = 9.98 KK385 pKa = 8.42 YY386 pKa = 10.71 KK387 pKa = 11.29 MLDDD391 pKa = 3.61 YY392 pKa = 11.3 DDD394 pKa = 5.84 NSQHHH399 pKa = 6.71 IQSMRR404 pKa = 11.84 LIFEEE409 pKa = 4.35 MKKK412 pKa = 10.79 EE413 pKa = 4.04 LPPEEE418 pKa = 4.42 SFALDDD424 pKa = 3.01 CIASFDDD431 pKa = 3.88 MQTSDDD437 pKa = 3.33 RR438 pKa = 11.84 KKK440 pKa = 5.64 TATLPSGHHH449 pKa = 7.21 ATTFINTVLNWCYYY463 pKa = 9.23 QMVGLKKK470 pKa = 9.52 DDD472 pKa = 4.16 FMCAGDDD479 pKa = 4.46 DD480 pKa = 4.1 ILMSQEEE487 pKa = 4.57 ISLAPILKKK496 pKa = 10.02 QFKKK500 pKa = 10.32 NPSKKK505 pKa = 10.84 STGTRR510 pKa = 11.84 GEEE513 pKa = 4.34 LRR515 pKa = 11.84 KKK517 pKa = 9.45 HH518 pKa = 4.23 YY519 pKa = 10.01 EEE521 pKa = 5.28 GVFAYYY527 pKa = 8.75 CRR529 pKa = 11.84 AIASLVSGNWLSEEE543 pKa = 4.12 LRR545 pKa = 11.84 DDD547 pKa = 3.6 TPILVPIQNGIDDD560 pKa = 3.56 LRR562 pKa = 11.84 SRR564 pKa = 11.84 AGLLGVPWKKK574 pKa = 10.76 GLSEEE579 pKa = 4.86 IEEE582 pKa = 4.55 EEE584 pKa = 4.51 IPKKK588 pKa = 9.28 DD589 pKa = 3.53 SMALLNSHHH598 pKa = 6.99 AGPGLITRR606 pKa = 11.84 DDD608 pKa = 3.58 YY609 pKa = 11.53 SFTVTPTPPKKK620 pKa = 9.61 HHH622 pKa = 6.77 SLEEE626 pKa = 4.23 YY627 pKa = 9.01 ATRR630 pKa = 11.84 HHH632 pKa = 6.01 LQDDD636 pKa = 3.62 CKKK639 pKa = 9.78 HH640 pKa = 5.67 PWKKK644 pKa = 10.56 LTANEEE650 pKa = 4.37 NKKK653 pKa = 10.3 GQQIKKK659 pKa = 10.35 KK660 pKa = 8.89 SHHH663 pKa = 5.28 HHH665 pKa = 5.12 SQTKKK670 pKa = 7.74 TYYY673 pKa = 8.04 KK674 pKa = 10.31 VYYY677 pKa = 9.85 EE678 pKa = 4.11 FKKK681 pKa = 10.82 SGLPTVLSEEE691 pKa = 4.36 SQSALSLVWWQAMLKKK707 pKa = 10.25 EE708 pKa = 4.09 MQDDD712 pKa = 3.36 YY713 pKa = 8.34 TKKK716 pKa = 10.71 KK717 pKa = 10.21 KK718 pKa = 9.84 DD719 pKa = 3.19 HHH721 pKa = 6.25 YYY723 pKa = 9.96 CNACTSSVSGDDD735 pKa = 3.03 FLRR738 pKa = 11.84 ATSKKK743 pKa = 10.11 AGVLITSLISSSS
Molecular weight: 85.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.297
IPC2_protein 7.658
IPC_protein 7.454
Toseland 7.176
ProMoST 8.053
Dawson 8.185
Bjellqvist 8.551
Wikipedia 8.112
Rodwell 8.229
Grimsley 7.249
Solomon 8.229
Lehninger 8.244
Nozaki 8.712
DTASelect 8.287
Thurlkill 8.346
EMBOSS 8.39
Sillero 8.668
Patrickios 4.113
IPC_peptide 8.229
IPC2_peptide 7.483
IPC2.peptide.svr19 7.644
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1434
678
756
717.0
80.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.531 ± 1.119
1.604 ± 0.363
5.091 ± 0.142
4.463 ± 0.024
5.021 ± 0.559
5.439 ± 0.386
2.859 ± 0.036
6.206 ± 0.461
5.021 ± 1.129
9.484 ± 1.81
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.58 ± 0.141
4.393 ± 0.395
5.021 ± 0.285
3.347 ± 0.44
4.881 ± 0.46
8.926 ± 0.049
7.252 ± 0.64
5.509 ± 0.811
1.534 ± 0.319
3.835 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here