Hubei narna-like virus 19

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIH1|A0A1L3KIH1_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 19 OX=1922949 PE=4 SV=1
MM1 pKa = 7.24SGAMPKK7 pKa = 9.5HH8 pKa = 5.88CEE10 pKa = 3.85VRR12 pKa = 11.84KK13 pKa = 8.53VTLAALCRR21 pKa = 11.84PLVCTSSGRR30 pKa = 11.84LGVWDD35 pKa = 3.67VRR37 pKa = 11.84SGHH40 pKa = 6.56RR41 pKa = 11.84SDD43 pKa = 4.13PVRR46 pKa = 11.84KK47 pKa = 9.34SGRR50 pKa = 11.84VRR52 pKa = 11.84IRR54 pKa = 11.84SWDD57 pKa = 3.59PPRR60 pKa = 11.84LYY62 pKa = 10.01PGQCHH67 pKa = 5.81EE68 pKa = 4.6PRR70 pKa = 11.84APLAEE75 pKa = 4.61ADD77 pKa = 3.61GATRR81 pKa = 11.84TVSMSEE87 pKa = 3.88VTPITASSLIEE98 pKa = 4.14LGLLRR103 pKa = 11.84FGGPDD108 pKa = 3.14GLGPLLAEE116 pKa = 3.92VFLRR120 pKa = 11.84EE121 pKa = 4.44NIHH124 pKa = 5.75EE125 pKa = 4.43TTGDD129 pKa = 3.4AVRR132 pKa = 11.84SCDD135 pKa = 3.54EE136 pKa = 3.99TCQLDD141 pKa = 3.87SCLMMTGRR149 pKa = 11.84VTYY152 pKa = 10.28SGPNRR157 pKa = 11.84EE158 pKa = 4.54RR159 pKa = 11.84YY160 pKa = 7.8TIQPHH165 pKa = 5.91PAFSIWQTGSTQIQRR180 pKa = 11.84EE181 pKa = 4.3MWWNSSGEE189 pKa = 4.16PNHH192 pKa = 6.53KK193 pKa = 9.08VTVIQPYY200 pKa = 9.87GSVRR204 pKa = 11.84LAALARR210 pKa = 11.84YY211 pKa = 8.06YY212 pKa = 10.01IRR214 pKa = 11.84SLGEE218 pKa = 3.68PSSQGLNQGALTGSRR233 pKa = 11.84TNYY236 pKa = 9.72ASNRR240 pKa = 11.84KK241 pKa = 8.1VLKK244 pKa = 10.06DD245 pKa = 3.31LHH247 pKa = 7.13AGYY250 pKa = 10.24YY251 pKa = 9.87INWDD255 pKa = 3.65EE256 pKa = 4.22LTQGSLQGVGKK267 pKa = 9.21LLCSEE272 pKa = 4.73TVRR275 pKa = 11.84EE276 pKa = 4.16THH278 pKa = 6.76CPLGKK283 pKa = 9.91SAAGARR289 pKa = 11.84NDD291 pKa = 3.23SFRR294 pKa = 11.84PVYY297 pKa = 10.79GEE299 pKa = 3.56AHH301 pKa = 7.08LLHH304 pKa = 7.2DD305 pKa = 3.78IEE307 pKa = 5.16VNFRR311 pKa = 11.84PWEE314 pKa = 4.35DD315 pKa = 3.63PEE317 pKa = 4.5EE318 pKa = 4.41VIPSDD323 pKa = 3.18NMRR326 pKa = 11.84VRR328 pKa = 11.84TISSFLHH335 pKa = 5.78AKK337 pKa = 9.95SVEE340 pKa = 4.03KK341 pKa = 10.96GKK343 pKa = 11.18GEE345 pKa = 3.79EE346 pKa = 4.36AEE348 pKa = 4.73RR349 pKa = 11.84SAHH352 pKa = 5.94MNTAGTRR359 pKa = 11.84DD360 pKa = 3.8DD361 pKa = 3.45GTIRR365 pKa = 11.84ITEE368 pKa = 3.94LHH370 pKa = 6.16RR371 pKa = 11.84AKK373 pKa = 10.33HH374 pKa = 5.92DD375 pKa = 3.49SAVRR379 pKa = 11.84INVRR383 pKa = 11.84HH384 pKa = 4.91NMMWLEE390 pKa = 3.8EE391 pKa = 4.29SGPEE395 pKa = 3.58RR396 pKa = 11.84LRR398 pKa = 11.84INVGNVVCCIGKK410 pKa = 8.41VTANADD416 pKa = 3.71TVPVEE421 pKa = 4.13RR422 pKa = 11.84RR423 pKa = 11.84PNGAKK428 pKa = 9.91KK429 pKa = 10.69LNIIGLSIHH438 pKa = 6.15VGPRR442 pKa = 11.84LIRR445 pKa = 11.84HH446 pKa = 6.22HH447 pKa = 6.81CPHH450 pKa = 6.99LSSYY454 pKa = 11.1YY455 pKa = 8.42ITNDD459 pKa = 3.07EE460 pKa = 4.24GSSGEE465 pKa = 3.96LCKK468 pKa = 10.73NPRR471 pKa = 11.84FISFFRR477 pKa = 11.84DD478 pKa = 2.89RR479 pKa = 11.84HH480 pKa = 5.43HH481 pKa = 7.16VGNPLLLWRR490 pKa = 11.84DD491 pKa = 3.54GTSCHH496 pKa = 6.69IDD498 pKa = 3.31SVVKK502 pKa = 10.13GFRR505 pKa = 11.84DD506 pKa = 3.56PRR508 pKa = 11.84VEE510 pKa = 3.27IALRR514 pKa = 11.84KK515 pKa = 9.41RR516 pKa = 11.84FHH518 pKa = 6.11TFEE521 pKa = 3.73IALRR525 pKa = 11.84FCIADD530 pKa = 3.63QLLHH534 pKa = 6.15TSSFEE539 pKa = 4.16DD540 pKa = 3.15RR541 pKa = 11.84CSLRR545 pKa = 11.84RR546 pKa = 11.84GVLIRR551 pKa = 11.84CPRR554 pKa = 11.84NFLFKK559 pKa = 10.42EE560 pKa = 3.8VGIISRR566 pKa = 11.84EE567 pKa = 3.91LPGPIVNVVGQFHH580 pKa = 7.2VDD582 pKa = 3.27ITWRR586 pKa = 11.84SCDD589 pKa = 3.55DD590 pKa = 3.97RR591 pKa = 11.84SVCLYY596 pKa = 11.17GLGLDD601 pKa = 3.23KK602 pKa = 10.92CIPFRR607 pKa = 11.84EE608 pKa = 4.33TSANARR614 pKa = 11.84NLRR617 pKa = 11.84PSLGVAHH624 pKa = 6.77VPGQPFLEE632 pKa = 4.87GTRR635 pKa = 11.84HH636 pKa = 3.64VTLRR640 pKa = 11.84SGSSTSHH647 pKa = 6.53RR648 pKa = 11.84PLAVLEE654 pKa = 4.42AEE656 pKa = 4.41LLQLLGYY663 pKa = 8.3VVPTPRR669 pKa = 11.84VTRR672 pKa = 11.84TAEE675 pKa = 4.0EE676 pKa = 4.27PTCEE680 pKa = 4.0YY681 pKa = 10.74LCRR684 pKa = 11.84DD685 pKa = 5.08DD686 pKa = 4.45ISKK689 pKa = 10.78LLVVSKK695 pKa = 10.81YY696 pKa = 11.02NIGHH700 pKa = 6.41NSFPDD705 pKa = 3.14RR706 pKa = 11.84VRR708 pKa = 11.84ISNSIPIRR716 pKa = 11.84QGRR719 pKa = 11.84HH720 pKa = 3.53WQLMPMSCPNNTRR733 pKa = 11.84GNRR736 pKa = 11.84PRR738 pKa = 11.84QYY740 pKa = 11.05VRR742 pKa = 11.84WW743 pKa = 3.79

Molecular weight: 83.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIH1|A0A1L3KIH1_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 19 OX=1922949 PE=4 SV=1
MM1 pKa = 6.5TTSSRR6 pKa = 11.84YY7 pKa = 9.24FDD9 pKa = 4.22LPSNILSWPVTPGVVGAGHH28 pKa = 6.25WHH30 pKa = 5.45QLPVSALTDD39 pKa = 3.65RR40 pKa = 11.84DD41 pKa = 3.35GVRR44 pKa = 11.84YY45 pKa = 10.37SNSVWKK51 pKa = 10.52AVVTNIVLAHH61 pKa = 5.85NKK63 pKa = 9.49KK64 pKa = 9.67FRR66 pKa = 11.84NVVPAKK72 pKa = 10.38IFAGWLLRR80 pKa = 11.84CSRR83 pKa = 11.84NAGGRR88 pKa = 11.84YY89 pKa = 8.86YY90 pKa = 10.6VAKK93 pKa = 10.12QLKK96 pKa = 8.74QLSFEE101 pKa = 3.99YY102 pKa = 10.28RR103 pKa = 11.84QWSVTGRR110 pKa = 11.84RR111 pKa = 11.84PTPKK115 pKa = 9.84SDD117 pKa = 3.46VPRR120 pKa = 11.84TFQKK124 pKa = 9.63WLAGHH129 pKa = 5.91VCHH132 pKa = 7.11AEE134 pKa = 3.48ARR136 pKa = 11.84AQIARR141 pKa = 11.84IGRR144 pKa = 11.84CLPEE148 pKa = 5.92GDD150 pKa = 4.26TLVQSKK156 pKa = 10.29AIKK159 pKa = 8.8AHH161 pKa = 6.2RR162 pKa = 11.84SIVTRR167 pKa = 11.84PPCDD171 pKa = 3.42VNMEE175 pKa = 4.25LADD178 pKa = 5.65DD179 pKa = 4.15IYY181 pKa = 10.19YY182 pKa = 9.02WARR185 pKa = 11.84KK186 pKa = 9.02FSTDD190 pKa = 2.95NAHH193 pKa = 6.75LFKK196 pKa = 11.25EE197 pKa = 4.54EE198 pKa = 4.12VSWAPNEE205 pKa = 4.05NSASQGATILEE216 pKa = 4.33GGRR219 pKa = 11.84MEE221 pKa = 5.04EE222 pKa = 4.95LIRR225 pKa = 11.84DD226 pKa = 4.17AKK228 pKa = 11.02AEE230 pKa = 4.04SDD232 pKa = 3.91FEE234 pKa = 4.7SVKK237 pKa = 10.97SLAEE241 pKa = 4.0GDD243 pKa = 3.51FDD245 pKa = 3.86PWVSKK250 pKa = 11.06ALDD253 pKa = 3.59DD254 pKa = 4.11AVYY257 pKa = 7.74MTACTVPPKK266 pKa = 10.51KK267 pKa = 10.22EE268 pKa = 3.99WIPDD272 pKa = 3.35VMAIAEE278 pKa = 4.35KK279 pKa = 10.26GYY281 pKa = 10.07KK282 pKa = 9.95ARR284 pKa = 11.84VLTKK288 pKa = 9.57FPAAALVVGDD298 pKa = 3.76VIRR301 pKa = 11.84RR302 pKa = 11.84QMWAMVSDD310 pKa = 4.36KK311 pKa = 10.41PWADD315 pKa = 3.72MDD317 pKa = 4.04RR318 pKa = 11.84QPDD321 pKa = 3.75DD322 pKa = 3.77VKK324 pKa = 10.88FLSTIRR330 pKa = 11.84SALDD334 pKa = 2.96RR335 pKa = 11.84HH336 pKa = 6.4GICVSSDD343 pKa = 2.8LSNATDD349 pKa = 4.52YY350 pKa = 11.04IPHH353 pKa = 7.33IYY355 pKa = 10.07AQALWAGLLEE365 pKa = 4.31PHH367 pKa = 6.6HH368 pKa = 6.71VMPYY372 pKa = 9.34INSYY376 pKa = 8.58CAIMFSSMKK385 pKa = 10.09LRR387 pKa = 11.84YY388 pKa = 8.99PDD390 pKa = 3.5GTVVTSARR398 pKa = 11.84GIHH401 pKa = 6.19MGTPLSFLTLTLLHH415 pKa = 6.67RR416 pKa = 11.84FCVEE420 pKa = 3.28KK421 pKa = 10.54AGYY424 pKa = 9.96GSYY427 pKa = 10.26PHH429 pKa = 7.66IIRR432 pKa = 11.84GDD434 pKa = 3.67DD435 pKa = 3.45LLGIFPRR442 pKa = 11.84PEE444 pKa = 3.57VYY446 pKa = 10.42FNVMQQVGFSINRR459 pKa = 11.84AKK461 pKa = 10.25TIISRR466 pKa = 11.84TGGTFAEE473 pKa = 4.08RR474 pKa = 11.84TVRR477 pKa = 11.84FSHH480 pKa = 6.75SLATQEE486 pKa = 4.97LSNPLKK492 pKa = 9.94RR493 pKa = 11.84TLGQFIPVNIISSVKK508 pKa = 9.87VLQDD512 pKa = 3.53LPVGGVVRR520 pKa = 11.84ATPGKK525 pKa = 9.75GSLVKK530 pKa = 10.72ALGRR534 pKa = 11.84WFSQTSNIVPRR545 pKa = 11.84QRR547 pKa = 11.84RR548 pKa = 11.84KK549 pKa = 9.53AYY551 pKa = 9.8RR552 pKa = 11.84AIGLNHH558 pKa = 6.97GDD560 pKa = 3.48LVIRR564 pKa = 11.84LSTTVPPHH572 pKa = 6.66LPLDD576 pKa = 4.05LGGAGLPDD584 pKa = 3.09RR585 pKa = 11.84KK586 pKa = 10.62GRR588 pKa = 11.84VGLNGVPFAIRR599 pKa = 11.84AAIGHH604 pKa = 6.53AASHH608 pKa = 6.74HH609 pKa = 4.86EE610 pKa = 4.26TAVKK614 pKa = 9.01LTGLIARR621 pKa = 11.84TDD623 pKa = 3.51GVSRR627 pKa = 11.84GFVDD631 pKa = 3.93VFSKK635 pKa = 10.98KK636 pKa = 10.07DD637 pKa = 3.71FRR639 pKa = 11.84EE640 pKa = 4.04QWSKK644 pKa = 11.35SIWTTEE650 pKa = 3.79PQEE653 pKa = 4.09TEE655 pKa = 4.49FYY657 pKa = 10.71QRR659 pKa = 11.84TRR661 pKa = 11.84RR662 pKa = 11.84YY663 pKa = 7.75WRR665 pKa = 11.84YY666 pKa = 8.76FGHH669 pKa = 7.68RR670 pKa = 11.84DD671 pKa = 3.3RR672 pKa = 11.84PSRR675 pKa = 11.84PISFRR680 pKa = 11.84QWRR683 pKa = 11.84SGLMTLPRR691 pKa = 11.84VKK693 pKa = 10.55ARR695 pKa = 11.84WVPRR699 pKa = 11.84SNSDD703 pKa = 3.12PSRR706 pKa = 11.84LANRR710 pKa = 11.84IRR712 pKa = 11.84SMTGTYY718 pKa = 9.48IPYY721 pKa = 7.82TQPTTGSTYY730 pKa = 9.89QWAAKK735 pKa = 10.17CGG737 pKa = 3.52

Molecular weight: 82.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1480

737

743

740.0

83.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.824 ± 1.111

2.162 ± 0.658

4.662 ± 0.251

4.662 ± 0.888

3.243 ± 0.389

7.432 ± 0.264

3.446 ± 0.417

5.27 ± 0.015

3.986 ± 0.723

8.243 ± 0.546

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.824 ± 0.148

3.581 ± 0.512

6.149 ± 0.03

2.838 ± 0.293

9.392 ± 0.59

7.973 ± 0.167

5.878 ± 0.254

7.5 ± 0.164

2.162 ± 0.386

2.77 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski