Fall chinook aquareovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Aquareovirus; unclassified Aquareovirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6PCW9|A0A1S6PCW9_9REOV NS4 OS=Fall chinook aquareovirus OX=1963254 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 5.66FKK4 pKa = 10.62RR5 pKa = 11.84GVPTVSVHH13 pKa = 7.23RR14 pKa = 11.84DD15 pKa = 3.02MTEE18 pKa = 3.89FPEE21 pKa = 4.64VATPPIDD28 pKa = 5.4AIFDD32 pKa = 3.61QAQPYY37 pKa = 9.35CDD39 pKa = 4.32HH40 pKa = 7.04LGKK43 pKa = 9.76PLISVYY49 pKa = 10.55DD50 pKa = 3.67GRR52 pKa = 11.84LVPISGPTHH61 pKa = 7.2LEE63 pKa = 4.13DD64 pKa = 4.64EE65 pKa = 4.99DD66 pKa = 6.23DD67 pKa = 3.98YY68 pKa = 11.94EE69 pKa = 4.23PWQVHH74 pKa = 5.09GQLQTLSAHH83 pKa = 5.88SKK85 pKa = 9.04WPEE88 pKa = 3.91SVPNWTSHH96 pKa = 4.76PHH98 pKa = 5.02IQVFSNMSPYY108 pKa = 11.34ANIPGPSPYY117 pKa = 10.34KK118 pKa = 10.38FEE120 pKa = 4.2SWPAALAPHH129 pKa = 6.99GGLSAWDD136 pKa = 4.05LLWRR140 pKa = 11.84CAQLRR145 pKa = 11.84PNRR148 pKa = 11.84LPVYY152 pKa = 10.41SDD154 pKa = 3.79AFLLSTLRR162 pKa = 11.84LSGKK166 pKa = 8.05QVFEE170 pKa = 4.52LPMLDD175 pKa = 3.69SVADD179 pKa = 3.78MLSWAGWNADD189 pKa = 3.86MLCGLDD195 pKa = 3.54LCQTEE200 pKa = 4.26GMMAIPDD207 pKa = 4.56PGDD210 pKa = 3.12EE211 pKa = 4.33HH212 pKa = 8.32LPLLVEE218 pKa = 3.73QTAWFDD224 pKa = 3.75YY225 pKa = 10.5IIRR228 pKa = 11.84LPFDD232 pKa = 3.62TTHH235 pKa = 6.77HH236 pKa = 6.72LGLGLTDD243 pKa = 3.89DD244 pKa = 4.76QLWSHH249 pKa = 7.52PFTVLCFLRR258 pKa = 11.84WRR260 pKa = 11.84AAWPDD265 pKa = 3.72LYY267 pKa = 11.45

Molecular weight: 30.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6PCW6|A0A1S6PCW6_9REOV NS2 OS=Fall chinook aquareovirus OX=1963254 PE=4 SV=1
MM1 pKa = 7.83PCGDD5 pKa = 4.48TIATTFQYY13 pKa = 10.51TVLQVDD19 pKa = 4.62RR20 pKa = 11.84SCCISTSVTATSEE33 pKa = 4.11ATSWALPPILACCCCVCCCAAAGYY57 pKa = 9.91AISRR61 pKa = 11.84GSCPCITRR69 pKa = 11.84RR70 pKa = 11.84LDD72 pKa = 3.18VLRR75 pKa = 11.84GTGFTTEE82 pKa = 3.48HH83 pKa = 6.57SLRR86 pKa = 11.84ANRR89 pKa = 11.84KK90 pKa = 8.2SISRR94 pKa = 11.84SHH96 pKa = 6.93HH97 pKa = 5.8SRR99 pKa = 11.84SSDD102 pKa = 3.2SRR104 pKa = 11.84STSHH108 pKa = 6.97HH109 pKa = 6.83PALEE113 pKa = 4.27LPGYY117 pKa = 10.18RR118 pKa = 11.84YY119 pKa = 8.51TDD121 pKa = 2.71VSYY124 pKa = 11.31AHH126 pKa = 7.21RR127 pKa = 11.84SEE129 pKa = 4.39SDD131 pKa = 3.35SASEE135 pKa = 3.9LSSAEE140 pKa = 4.16SEE142 pKa = 4.56VITYY146 pKa = 7.5QPRR149 pKa = 11.84PFITHH154 pKa = 7.13LEE156 pKa = 4.26SQSSCGTCLDD166 pKa = 3.82HH167 pKa = 6.33QQNGIQEE174 pKa = 4.38RR175 pKa = 11.84SPNCVSPSRR184 pKa = 11.84HH185 pKa = 5.61DD186 pKa = 4.15RR187 pKa = 11.84IPRR190 pKa = 11.84SGHH193 pKa = 4.04PTYY196 pKa = 10.81RR197 pKa = 11.84CDD199 pKa = 4.09LRR201 pKa = 11.84PGSTLLL207 pKa = 4.01

Molecular weight: 22.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7502

207

1295

625.2

68.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.797 ± 0.468

1.293 ± 0.271

5.319 ± 0.226

3.159 ± 0.231

3.479 ± 0.244

5.678 ± 0.216

2.706 ± 0.261

5.385 ± 0.251

2.319 ± 0.347

9.717 ± 0.439

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.268

4.132 ± 0.275

7.665 ± 0.348

3.759 ± 0.292

5.305 ± 0.319

8.438 ± 0.572

7.305 ± 0.35

7.265 ± 0.318

1.533 ± 0.218

2.933 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski