Blastococcus sp. TF02-9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Blastococcus; unclassified Blastococcus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366ZEM5|A0A366ZEM5_9ACTN Isocitrate lyase/phosphoenolpyruvate mutase family protein OS=Blastococcus sp. TF02-9 OX=2250576 GN=DQ239_15900 PE=4 SV=1
MM1 pKa = 7.33NLGPVSRR8 pKa = 11.84SAAALAAVLLAATAGCSTKK27 pKa = 10.57APEE30 pKa = 4.14SSGGGGGGGDD40 pKa = 3.48AADD43 pKa = 3.63VQTDD47 pKa = 3.52VGVEE51 pKa = 3.98GTTIQLGVLTDD62 pKa = 3.65LTGVFAALGKK72 pKa = 10.36DD73 pKa = 3.2LTNANTLYY81 pKa = 10.3WEE83 pKa = 4.94DD84 pKa = 3.63NQVCDD89 pKa = 3.95TYY91 pKa = 11.53DD92 pKa = 3.34VEE94 pKa = 6.66LDD96 pKa = 3.83VQDD99 pKa = 3.57TGYY102 pKa = 10.9VPQTGVQLYY111 pKa = 10.71SGMKK115 pKa = 10.2DD116 pKa = 3.1SILAMQQTIGSPINTALAPEE136 pKa = 4.36YY137 pKa = 10.69EE138 pKa = 4.25SDD140 pKa = 3.81QIVNFPSAWSKK151 pKa = 9.42TLTEE155 pKa = 4.03IPGTGVVGATYY166 pKa = 10.42DD167 pKa = 3.59VEE169 pKa = 4.1ISNGYY174 pKa = 10.44DD175 pKa = 3.27YY176 pKa = 11.59LLQQGLLKK184 pKa = 10.96EE185 pKa = 4.43GDD187 pKa = 4.18TVGHH191 pKa = 6.94IYY193 pKa = 10.84FEE195 pKa = 4.56GEE197 pKa = 3.69YY198 pKa = 10.43GANGLAGSQAVAEE211 pKa = 4.29AKK213 pKa = 10.25GLNIVEE219 pKa = 4.25AQIKK223 pKa = 8.41STDD226 pKa = 3.31TDD228 pKa = 2.93MSAQVTQFKK237 pKa = 10.52AAGVKK242 pKa = 10.3AIALTVAPAQTTSVAAVAQAQGLDD266 pKa = 3.5VPMLGNNPVFAPGLLDD282 pKa = 4.95GPTADD287 pKa = 3.04WLKK290 pKa = 8.13THH292 pKa = 7.25LYY294 pKa = 8.55VASPVSSFADD304 pKa = 3.32HH305 pKa = 7.6ADD307 pKa = 3.68LLDD310 pKa = 4.93AYY312 pKa = 9.7QQAYY316 pKa = 9.51PDD318 pKa = 3.81VTPSLGVLVGYY329 pKa = 10.9GMATLMNQVLDD340 pKa = 4.18AACEE344 pKa = 4.07NGDD347 pKa = 3.68LTRR350 pKa = 11.84AGLVEE355 pKa = 4.64AFDD358 pKa = 4.06GLEE361 pKa = 4.19DD362 pKa = 3.77VDD364 pKa = 3.85TGGLVVPIRR373 pKa = 11.84GFEE376 pKa = 4.06NGKK379 pKa = 9.95SPSLEE384 pKa = 3.96SFVLVPADD392 pKa = 3.66EE393 pKa = 4.81PGGAKK398 pKa = 9.45VAQDD402 pKa = 3.22AFEE405 pKa = 5.06GEE407 pKa = 4.32FAADD411 pKa = 3.11IAGG414 pKa = 3.47

Molecular weight: 42.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366ZVM9|A0A366ZVM9_9ACTN Type II toxin-antitoxin system PemK/MazF family toxin OS=Blastococcus sp. TF02-9 OX=2250576 GN=DQ239_00310 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PGRR8 pKa = 11.84RR9 pKa = 11.84PRR11 pKa = 11.84ARR13 pKa = 11.84ARR15 pKa = 11.84PGGAGAGALPGRR27 pKa = 11.84RR28 pKa = 11.84ARR30 pKa = 11.84GGGAGRR36 pKa = 11.84RR37 pKa = 11.84GLAGLARR44 pKa = 11.84LALSSRR50 pKa = 11.84RR51 pKa = 11.84VMPHH55 pKa = 5.95PSLRR59 pKa = 11.84ARR61 pKa = 11.84PAQAHH66 pKa = 4.82RR67 pKa = 11.84RR68 pKa = 11.84IRR70 pKa = 11.84RR71 pKa = 11.84CRR73 pKa = 11.84PARR76 pKa = 11.84PP77 pKa = 3.31

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3851

0

3851

1224882

32

2508

318.1

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.249 ± 0.061

0.679 ± 0.009

6.368 ± 0.032

5.69 ± 0.04

2.556 ± 0.024

9.574 ± 0.039

1.949 ± 0.018

2.88 ± 0.028

1.421 ± 0.025

10.639 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.622 ± 0.017

1.468 ± 0.016

6.182 ± 0.034

2.726 ± 0.022

8.167 ± 0.048

4.918 ± 0.027

5.988 ± 0.038

9.676 ± 0.04

1.484 ± 0.016

1.761 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski