Trichoderma arundinaceum
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10471 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A395NDV8|A0A395NDV8_TRIAR Major facilitator superfamily transporter OS=Trichoderma arundinaceum OX=490622 GN=TARUN_8314 PE=4 SV=1
MM1 pKa = 7.25 QLTTAIFTTLCLALSATADD20 pKa = 3.45 QRR22 pKa = 11.84 ICFPLPGDD30 pKa = 3.99 EE31 pKa = 4.1 NTIPQAILDD40 pKa = 4.53 LDD42 pKa = 4.09 SQTKK46 pKa = 10.25 LNWAATLCSQLTFPSDD62 pKa = 3.39 GLQEE66 pKa = 4.52 AKK68 pKa = 9.41 TAAADD73 pKa = 4.91 GILGDD78 pKa = 4.41 DD79 pKa = 3.37 GRR81 pKa = 11.84 LYY83 pKa = 10.91 GLEE86 pKa = 4.09 LAVHH90 pKa = 7.59 DD91 pKa = 4.65 IQTEE95 pKa = 4.19 DD96 pKa = 3.28 NCRR99 pKa = 11.84 VNANALLGAGACPGGGLLTLSGPLEE124 pKa = 3.79 QWSYY128 pKa = 9.21 ITALNN133 pKa = 3.6
Molecular weight: 13.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.872
Toseland 3.656
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.834
Sillero 3.986
Patrickios 0.846
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A395NKJ3|A0A395NKJ3_TRIAR Methylisocitrate lyase OS=Trichoderma arundinaceum OX=490622 GN=TARUN_5645 PE=4 SV=1
PP1 pKa = 7.05 KK2 pKa = 10.06 RR3 pKa = 11.84 AGGSSRR9 pKa = 11.84 GGPRR13 pKa = 11.84 GGGRR17 pKa = 11.84 GGFSRR22 pKa = 11.84 GGRR25 pKa = 11.84 GGPGGFGGRR34 pKa = 11.84 GGRR37 pKa = 11.84 GGSGGFSRR45 pKa = 11.84 GGGGGFGGRR54 pKa = 11.84 GGRR57 pKa = 11.84 GGSGGFSRR65 pKa = 11.84 GGAPGGGRR73 pKa = 11.84 GRR75 pKa = 11.84 GGFSRR80 pKa = 11.84 GGRR83 pKa = 3.49
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.403
IPC_protein 13.013
Toseland 13.173
ProMoST 13.685
Dawson 13.173
Bjellqvist 13.173
Wikipedia 13.656
Rodwell 12.705
Grimsley 13.217
Solomon 13.671
Lehninger 13.583
Nozaki 13.173
DTASelect 13.173
Thurlkill 13.173
EMBOSS 13.671
Sillero 13.173
Patrickios 12.427
IPC_peptide 13.685
IPC2_peptide 12.676
IPC2.peptide.svr19 9.322
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10471
0
10471
5304629
18
23058
506.6
55.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.923 ± 0.025
1.176 ± 0.01
5.786 ± 0.018
6.168 ± 0.025
3.709 ± 0.014
6.94 ± 0.03
2.35 ± 0.011
5.1 ± 0.02
4.862 ± 0.022
8.902 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.175 ± 0.011
3.699 ± 0.015
5.953 ± 0.026
4.052 ± 0.017
5.971 ± 0.021
8.259 ± 0.029
5.74 ± 0.017
6.067 ± 0.018
1.459 ± 0.009
2.707 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here