Pistricoccus aurantiacus
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3396 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8SWT2|A0A5B8SWT2_9GAMM Respiratory nitrate reductase subunit beta OS=Pistricoccus aurantiacus OX=1883414 GN=FGL86_17045 PE=4 SV=1
MM1 pKa = 7.72 KK2 pKa = 9.1 KK3 pKa = 8.18 TLLVTAIAGALAASAGAASAATVYY27 pKa = 10.76 NQDD30 pKa = 3.0 GTKK33 pKa = 10.37 LDD35 pKa = 3.96 LYY37 pKa = 11.59 GNIQLAYY44 pKa = 9.77 KK45 pKa = 10.32 SIEE48 pKa = 3.92 TAQFEE53 pKa = 4.54 DD54 pKa = 4.08 VPGVGNDD61 pKa = 2.85 IYY63 pKa = 11.0 QGTEE67 pKa = 3.7 AEE69 pKa = 5.87 DD70 pKa = 4.09 EE71 pKa = 4.25 IFDD74 pKa = 3.91 NGSTIGVTGEE84 pKa = 3.86 HH85 pKa = 6.64 VIMNGLTGYY94 pKa = 10.16 FKK96 pKa = 11.28 AEE98 pKa = 3.84 WEE100 pKa = 4.13 FDD102 pKa = 3.07 ADD104 pKa = 3.83 QAKK107 pKa = 9.67 GAPGGINTGDD117 pKa = 3.29 QAYY120 pKa = 10.61 LGLKK124 pKa = 10.26 GNFGDD129 pKa = 4.77 ARR131 pKa = 11.84 LGSWDD136 pKa = 4.23 PLIDD140 pKa = 5.15 DD141 pKa = 5.78 WIQDD145 pKa = 3.91 PISNNEE151 pKa = 3.8 YY152 pKa = 10.85 FDD154 pKa = 4.5 VSDD157 pKa = 3.66 SNGRR161 pKa = 11.84 ILGVEE166 pKa = 3.73 NRR168 pKa = 11.84 EE169 pKa = 3.98 GDD171 pKa = 3.4 KK172 pKa = 10.64 LQYY175 pKa = 9.38 MSPSLGGLQFAVGTQYY191 pKa = 11.35 KK192 pKa = 10.49 GDD194 pKa = 3.89 AEE196 pKa = 4.28 FDD198 pKa = 3.55 GDD200 pKa = 4.16 DD201 pKa = 3.94 TVTGTNSLGDD211 pKa = 4.23 EE212 pKa = 4.11 ITVTGEE218 pKa = 3.88 DD219 pKa = 3.29 SSNASFFGGLKK230 pKa = 10.2 YY231 pKa = 10.46 EE232 pKa = 4.19 VGNFSIAAVYY242 pKa = 10.87 DD243 pKa = 3.62 NLDD246 pKa = 3.95 NNDD249 pKa = 3.34 GSYY252 pKa = 10.95 SAVDD256 pKa = 3.24 SDD258 pKa = 4.74 GNDD261 pKa = 2.93 IGSGDD266 pKa = 3.85 FEE268 pKa = 6.42 AGEE271 pKa = 4.32 QYY273 pKa = 10.94 GITGQYY279 pKa = 8.27 TWDD282 pKa = 3.69 TLRR285 pKa = 11.84 VALKK289 pKa = 10.78 AEE291 pKa = 4.31 RR292 pKa = 11.84 FKK294 pKa = 11.52 SDD296 pKa = 3.37 NDD298 pKa = 3.7 TLADD302 pKa = 3.54 TNYY305 pKa = 8.8 YY306 pKa = 10.89 ALGARR311 pKa = 11.84 YY312 pKa = 9.63 GYY314 pKa = 10.37 GNGMGDD320 pKa = 4.06 LYY322 pKa = 11.41 GSYY325 pKa = 10.49 QYY327 pKa = 11.58 VDD329 pKa = 3.21 VGGGDD334 pKa = 4.06 FLDD337 pKa = 4.24 TADD340 pKa = 4.96 DD341 pKa = 4.34 ALTSGDD347 pKa = 3.49 WPSEE351 pKa = 4.17 RR352 pKa = 11.84 EE353 pKa = 3.9 DD354 pKa = 3.44 EE355 pKa = 4.49 SYY357 pKa = 11.75 NEE359 pKa = 4.51 IILGATYY366 pKa = 10.51 NISDD370 pKa = 3.26 AMYY373 pKa = 9.27 TFVEE377 pKa = 4.5 GALYY381 pKa = 10.68 DD382 pKa = 3.78 RR383 pKa = 11.84 TDD385 pKa = 3.62 DD386 pKa = 3.94 EE387 pKa = 6.37 GDD389 pKa = 3.61 GVAVGAVYY397 pKa = 10.44 LFF399 pKa = 4.21
Molecular weight: 42.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.605
IPC_protein 3.643
Toseland 3.414
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.592
Rodwell 3.465
Grimsley 3.325
Solomon 3.643
Lehninger 3.605
Nozaki 3.757
DTASelect 4.024
Thurlkill 3.465
EMBOSS 3.605
Sillero 3.77
Patrickios 0.642
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A5B8SNT8|A0A5B8SNT8_9GAMM 30S ribosomal protein S20 OS=Pistricoccus aurantiacus OX=1883414 GN=rpsT PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.38 RR12 pKa = 11.84 KK13 pKa = 9.23 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3396
0
3396
1106925
28
3619
325.9
36.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.487 ± 0.046
0.945 ± 0.013
5.708 ± 0.038
6.497 ± 0.043
3.597 ± 0.024
7.881 ± 0.032
2.307 ± 0.022
5.089 ± 0.035
3.226 ± 0.032
11.522 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.449 ± 0.02
2.778 ± 0.02
4.868 ± 0.026
4.097 ± 0.03
7.17 ± 0.041
5.578 ± 0.025
4.926 ± 0.025
6.822 ± 0.035
1.549 ± 0.019
2.505 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here