Clostridium phage JD032

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Sherbrookevirus; unclassified Sherbrookevirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B7FSJ2|A0A6B7FSJ2_9CAUD Baseplate protein J OS=Clostridium phage JD032 OX=2530171 GN=JD032_36 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 4.89KK3 pKa = 10.3FIRR6 pKa = 11.84LDD8 pKa = 3.23YY9 pKa = 11.45DD10 pKa = 3.04KK11 pKa = 11.36GFRR14 pKa = 11.84GKK16 pKa = 9.75EE17 pKa = 4.03HH18 pKa = 6.43VSSATGDD25 pKa = 3.82GEE27 pKa = 4.23HH28 pKa = 6.7FEE30 pKa = 5.39AGISCYY36 pKa = 10.24KK37 pKa = 9.75ISKK40 pKa = 7.78EE41 pKa = 3.95KK42 pKa = 10.98CVDD45 pKa = 4.07AIINLCEE52 pKa = 3.76YY53 pKa = 8.8WFEE56 pKa = 4.51FAGEE60 pKa = 4.23CQFKK64 pKa = 11.12DD65 pKa = 3.22FDD67 pKa = 4.35INIFEE72 pKa = 4.33GCYY75 pKa = 9.26VGEE78 pKa = 4.31GASYY82 pKa = 11.0EE83 pKa = 4.15DD84 pKa = 4.32LATCEE89 pKa = 3.87KK90 pKa = 10.63HH91 pKa = 6.7LYY93 pKa = 8.5TVDD96 pKa = 3.22GSLFNEE102 pKa = 4.76VYY104 pKa = 10.54DD105 pKa = 4.23LYY107 pKa = 11.56YY108 pKa = 9.88MHH110 pKa = 6.84EE111 pKa = 4.48TYY113 pKa = 10.81LEE115 pKa = 4.05EE116 pKa = 4.29NGNVEE121 pKa = 4.3EE122 pKa = 5.38LEE124 pKa = 4.12EE125 pKa = 4.2NYY127 pKa = 10.19KK128 pKa = 10.86DD129 pKa = 3.78EE130 pKa = 5.0YY131 pKa = 10.02ITTEE135 pKa = 3.8EE136 pKa = 4.41FEE138 pKa = 4.55TKK140 pKa = 9.71IKK142 pKa = 10.83EE143 pKa = 3.99MFIKK147 pKa = 10.79YY148 pKa = 9.62LL149 pKa = 3.6

Molecular weight: 17.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B7FUN9|A0A6B7FUN9_9CAUD ATP-dependent Clp protease proteolytic subunit OS=Clostridium phage JD032 OX=2530171 GN=JD032_51 PE=3 SV=1
MM1 pKa = 7.5ARR3 pKa = 11.84RR4 pKa = 11.84HH5 pKa = 5.35IGAVISLKK13 pKa = 11.11DD14 pKa = 3.63NMSATMRR21 pKa = 11.84GIRR24 pKa = 11.84RR25 pKa = 11.84EE26 pKa = 3.74QKK28 pKa = 9.29QFQNEE33 pKa = 3.76VRR35 pKa = 11.84RR36 pKa = 11.84TRR38 pKa = 11.84NEE40 pKa = 3.3MRR42 pKa = 11.84SASRR46 pKa = 11.84EE47 pKa = 3.67RR48 pKa = 11.84MRR50 pKa = 11.84IRR52 pKa = 11.84MDD54 pKa = 3.02ATPAHH59 pKa = 6.58RR60 pKa = 11.84TIQDD64 pKa = 3.4LRR66 pKa = 11.84RR67 pKa = 11.84KK68 pKa = 8.49FAPLRR73 pKa = 11.84TKK75 pKa = 10.2LVKK78 pKa = 10.52AVVIKK83 pKa = 10.82DD84 pKa = 3.35LATEE88 pKa = 4.19KK89 pKa = 10.45IEE91 pKa = 4.58RR92 pKa = 11.84IKK94 pKa = 11.47SNVKK98 pKa = 10.41SFGKK102 pKa = 10.36FIARR106 pKa = 11.84PVVKK110 pKa = 10.65LKK112 pKa = 11.13DD113 pKa = 3.56EE114 pKa = 4.36TKK116 pKa = 11.22GMIDD120 pKa = 3.48KK121 pKa = 10.47IKK123 pKa = 10.98NRR125 pKa = 11.84LTSLSTIVPVGAAVGAAGMAVKK147 pKa = 10.35SGMEE151 pKa = 4.22LEE153 pKa = 4.18QQQISMRR160 pKa = 11.84HH161 pKa = 4.12FMGVGNKK168 pKa = 9.42GKK170 pKa = 10.31SSKK173 pKa = 10.53EE174 pKa = 3.68LDD176 pKa = 3.7GMSASYY182 pKa = 11.0LKK184 pKa = 10.53DD185 pKa = 3.17LRR187 pKa = 11.84NNANATPFEE196 pKa = 4.2TGEE199 pKa = 4.29VISAGTRR206 pKa = 11.84SLQIAGGNTKK216 pKa = 10.71DD217 pKa = 3.26AMQMVKK223 pKa = 10.34LAEE226 pKa = 4.7DD227 pKa = 3.64MAALNPGKK235 pKa = 8.56TVGDD239 pKa = 3.67AMEE242 pKa = 5.22ALADD246 pKa = 3.83MNIGEE251 pKa = 4.53MARR254 pKa = 11.84LTEE257 pKa = 4.43FGVKK261 pKa = 10.25ASSTDD266 pKa = 3.47DD267 pKa = 3.52PKK269 pKa = 11.15EE270 pKa = 3.99VQKK273 pKa = 11.17KK274 pKa = 10.2LEE276 pKa = 3.87TMYY279 pKa = 11.28AGGANKK285 pKa = 9.88LAEE288 pKa = 4.68SGSGLLSTIMGKK300 pKa = 10.14LKK302 pKa = 11.12SNIADD307 pKa = 3.2IGLGMLEE314 pKa = 4.03PLKK317 pKa = 10.63PVMTGLIGFIDD328 pKa = 3.69QASPKK333 pKa = 9.45ILEE336 pKa = 4.37VGTKK340 pKa = 7.49ITSGIGMAIGWIQQQMPTLAPIFQTAFGAVSSIVSTVAPIIGQVIGALSPVFMGLLSVASSALSGIASAVKK411 pKa = 8.64TVAPVVSSLISKK423 pKa = 7.97LAPIFLNVGSTLKK436 pKa = 11.14SMGKK440 pKa = 9.07IFKK443 pKa = 10.37NVFDD447 pKa = 4.39SVMKK451 pKa = 10.19IVKK454 pKa = 9.55KK455 pKa = 10.7ASDD458 pKa = 4.07FIKK461 pKa = 10.43PLVNGIIGATKK472 pKa = 10.29GISDD476 pKa = 3.93GVSWVAGKK484 pKa = 9.91LAGNATGTKK493 pKa = 8.26YY494 pKa = 10.08WSGGLSVVGEE504 pKa = 4.49HH505 pKa = 6.58GPEE508 pKa = 3.95LVSMPRR514 pKa = 11.84GSKK517 pKa = 10.23VFTNAEE523 pKa = 4.23SKK525 pKa = 11.46SMINKK530 pKa = 9.38SIPNFRR536 pKa = 11.84QVQGEE541 pKa = 4.12NTNYY545 pKa = 10.71NITIPKK551 pKa = 9.09IAEE554 pKa = 3.91QIVIRR559 pKa = 11.84EE560 pKa = 4.05DD561 pKa = 3.24ADD563 pKa = 3.29IEE565 pKa = 4.78RR566 pKa = 11.84ITSSLIKK573 pKa = 9.61KK574 pKa = 9.18IQMAKK579 pKa = 9.32MGGVVV584 pKa = 2.99

Molecular weight: 62.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10216

44

715

189.2

21.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.08 ± 0.369

1.087 ± 0.166

5.805 ± 0.268

9.397 ± 0.581

3.935 ± 0.185

5.844 ± 0.552

0.989 ± 0.134

9.25 ± 0.315

10.777 ± 0.498

8.232 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.428 ± 0.233

6.725 ± 0.374

2.163 ± 0.176

2.457 ± 0.187

3.534 ± 0.203

6.01 ± 0.37

5.707 ± 0.463

5.403 ± 0.256

0.969 ± 0.103

4.209 ± 0.351
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski