Acidihalobacter prosperus
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3188 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A6C2A5|A0A1A6C2A5_9GAMM Uncharacterized protein OS=Acidihalobacter prosperus OX=160660 GN=Thpro_022946 PE=4 SV=1
MM1 pKa = 6.38 STEE4 pKa = 3.97 VAEE7 pKa = 4.39 NTMPDD12 pKa = 3.64 PLLFTDD18 pKa = 4.27 SAASKK23 pKa = 10.33 VKK25 pKa = 10.41 EE26 pKa = 5.1 LIDD29 pKa = 4.16 EE30 pKa = 4.46 EE31 pKa = 4.58 NNPNLKK37 pKa = 10.34 LRR39 pKa = 11.84 VFVTGGGCSGFQYY52 pKa = 11.01 GFTFDD57 pKa = 4.44 EE58 pKa = 4.41 NVGDD62 pKa = 4.33 GDD64 pKa = 4.11 TTVEE68 pKa = 4.14 NAGVTLLIDD77 pKa = 3.74 PMSYY81 pKa = 10.31 QYY83 pKa = 11.58 LVGAEE88 pKa = 3.64 IDD90 pKa = 3.75 YY91 pKa = 10.81 TEE93 pKa = 4.44 GLEE96 pKa = 4.19 GAQFVIRR103 pKa = 11.84 NPNATTTCGCGSSFSVV119 pKa = 3.54
Molecular weight: 12.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.821
IPC_protein 3.732
Toseland 3.554
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.643
Sillero 3.859
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A1A6C4Q4|A0A1A6C4Q4_9GAMM Uncharacterized protein OS=Acidihalobacter prosperus OX=160660 GN=Thpro_021853 PE=3 SV=1
MM1 pKa = 7.14 NAAGTSASVDD11 pKa = 3.53 RR12 pKa = 11.84 RR13 pKa = 11.84 WPRR16 pKa = 11.84 AIVLVDD22 pKa = 3.23 MNAFFASIEE31 pKa = 3.99 QLDD34 pKa = 4.04 DD35 pKa = 3.71 PSLRR39 pKa = 11.84 GRR41 pKa = 11.84 PVAVTNGDD49 pKa = 3.12 QGTCIITCSYY59 pKa = 8.82 EE60 pKa = 3.49 ARR62 pKa = 11.84 AFGIHH67 pKa = 5.62 TGMRR71 pKa = 11.84 LPEE74 pKa = 3.87 ARR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 11.84 CPEE81 pKa = 4.82 LIRR84 pKa = 11.84 ITARR88 pKa = 11.84 SARR91 pKa = 11.84 YY92 pKa = 8.44 AQISARR98 pKa = 11.84 IMSALQAITPDD109 pKa = 3.1 IEE111 pKa = 4.35 VFSVDD116 pKa = 3.53 EE117 pKa = 4.54 AFLDD121 pKa = 3.66 VTRR124 pKa = 11.84 VQRR127 pKa = 11.84 LHH129 pKa = 6.52 GAPPVIAKK137 pKa = 9.62 KK138 pKa = 7.86 VQRR141 pKa = 11.84 LVRR144 pKa = 11.84 EE145 pKa = 4.28 VSGLPCSVGVAGDD158 pKa = 3.3 KK159 pKa = 7.75 TTAKK163 pKa = 9.36 WAAKK167 pKa = 7.74 QRR169 pKa = 11.84 KK170 pKa = 7.88 PEE172 pKa = 3.98 GVTVVPPWEE181 pKa = 3.81 TAARR185 pKa = 11.84 LRR187 pKa = 11.84 NVPVTEE193 pKa = 3.93 LCGVGRR199 pKa = 11.84 GIGSFLASRR208 pKa = 11.84 GVQTCGDD215 pKa = 3.9 MARR218 pKa = 11.84 LPVSVLAQRR227 pKa = 11.84 WGNVGRR233 pKa = 11.84 RR234 pKa = 11.84 IWLMAQGRR242 pKa = 11.84 DD243 pKa = 3.77 PEE245 pKa = 4.71 GVSPAVAPPKK255 pKa = 10.53 SVGHH259 pKa = 5.76 GKK261 pKa = 9.46 ILPPATRR268 pKa = 11.84 DD269 pKa = 3.4 PRR271 pKa = 11.84 LLQQYY276 pKa = 8.99 LEE278 pKa = 4.46 HH279 pKa = 6.59 MAQKK283 pKa = 10.12 VAARR287 pKa = 11.84 LRR289 pKa = 11.84 RR290 pKa = 11.84 NGLEE294 pKa = 3.83 AQQLFIGLRR303 pKa = 11.84 TDD305 pKa = 3.46 TGWLGGRR312 pKa = 11.84 YY313 pKa = 7.53 LTPRR317 pKa = 11.84 PTDD320 pKa = 3.83 DD321 pKa = 4.07 GQPIAALCRR330 pKa = 11.84 QVLARR335 pKa = 11.84 VWQGEE340 pKa = 4.38 GVHH343 pKa = 5.14 QVQVTALDD351 pKa = 3.91 PAPADD356 pKa = 3.51 VQLDD360 pKa = 3.88 LFEE363 pKa = 6.54 DD364 pKa = 4.49 PVAPTRR370 pKa = 11.84 QAVNRR375 pKa = 11.84 AVDD378 pKa = 4.56 RR379 pKa = 11.84 INRR382 pKa = 11.84 RR383 pKa = 11.84 FGGQAVATASLLGRR397 pKa = 11.84 VPLHH401 pKa = 6.99 DD402 pKa = 4.76 VIAPAWKK409 pKa = 9.06 PDD411 pKa = 3.79 GIRR414 pKa = 11.84 RR415 pKa = 11.84 TVV417 pKa = 2.61
Molecular weight: 45.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.355
IPC_protein 10.204
Toseland 10.584
ProMoST 10.335
Dawson 10.657
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.701
Grimsley 10.701
Solomon 10.818
Lehninger 10.789
Nozaki 10.599
DTASelect 10.394
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.613
Patrickios 10.409
IPC_peptide 10.833
IPC2_peptide 9.706
IPC2.peptide.svr19 8.633
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3188
0
3188
984843
37
1806
308.9
33.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.369 ± 0.062
0.884 ± 0.015
5.519 ± 0.035
5.652 ± 0.055
3.308 ± 0.028
8.504 ± 0.041
2.59 ± 0.023
4.671 ± 0.032
2.545 ± 0.034
11.287 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.381 ± 0.022
2.45 ± 0.028
5.224 ± 0.034
3.561 ± 0.03
7.972 ± 0.044
5.009 ± 0.03
4.838 ± 0.031
7.212 ± 0.034
1.402 ± 0.02
2.623 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here