Bovine papillomavirus type 5
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8BDG4|VE2_BPV5 Regulatory protein E2 OS=Bovine papillomavirus type 5 OX=40537 GN=E2 PE=3 SV=1
MM1 pKa = 7.53 AVRR4 pKa = 11.84 LRR6 pKa = 11.84 RR7 pKa = 11.84 VKK9 pKa = 10.35 RR10 pKa = 11.84 ANPYY14 pKa = 10.57 DD15 pKa = 4.17 LYY17 pKa = 9.98 RR18 pKa = 11.84 TCATGDD24 pKa = 3.64 CPQDD28 pKa = 3.23 VKK30 pKa = 11.53 DD31 pKa = 3.89 RR32 pKa = 11.84 FEE34 pKa = 5.45 HH35 pKa = 5.4 NTIADD40 pKa = 4.66 KK41 pKa = 10.59 ILKK44 pKa = 8.59 WGSAGVFLGGLGIGSTQARR63 pKa = 11.84 PGLGTYY69 pKa = 10.29 SPLGRR74 pKa = 11.84 GGVTGRR80 pKa = 11.84 IPVRR84 pKa = 11.84 GPGSTRR90 pKa = 11.84 PLGRR94 pKa = 11.84 PFSSGPIDD102 pKa = 3.6 TIGAGVRR109 pKa = 11.84 TSVEE113 pKa = 4.01 TSVTVPDD120 pKa = 4.04 VVAVLPEE127 pKa = 4.29 SPAVITPDD135 pKa = 3.88 SMPVDD140 pKa = 4.08 PGVGGLDD147 pKa = 3.01 ISAEE151 pKa = 4.08 IIEE154 pKa = 4.65 EE155 pKa = 4.01 PSLTFVEE162 pKa = 4.28 PHH164 pKa = 6.23 GPEE167 pKa = 4.02 DD168 pKa = 4.12 VAVLDD173 pKa = 4.23 VNPAEE178 pKa = 4.84 HH179 pKa = 7.09 DD180 pKa = 3.46 RR181 pKa = 11.84 SVYY184 pKa = 10.38 LSSSTTHH191 pKa = 6.51 HH192 pKa = 6.23 NPSFQGQVTVYY203 pKa = 9.67 TDD205 pKa = 2.84 IGEE208 pKa = 4.33 TSEE211 pKa = 4.42 TEE213 pKa = 3.7 NLLISGSNIGSSRR226 pKa = 11.84 GEE228 pKa = 4.17 EE229 pKa = 3.54 IQMQLFSGPKK239 pKa = 8.3 TSTPEE244 pKa = 3.36 TDD246 pKa = 3.01 AVTKK250 pKa = 10.53 VRR252 pKa = 11.84 GRR254 pKa = 11.84 ANWFSKK260 pKa = 10.24 RR261 pKa = 11.84 YY262 pKa = 6.12 YY263 pKa = 8.41 TQTSVRR269 pKa = 11.84 DD270 pKa = 3.44 PTFIQEE276 pKa = 4.09 PQTYY280 pKa = 9.32 FYY282 pKa = 11.25 GFEE285 pKa = 4.1 NPAYY289 pKa = 9.79 EE290 pKa = 4.59 PDD292 pKa = 3.74 PFEE295 pKa = 6.54 DD296 pKa = 3.81 SFDD299 pKa = 3.72 VQLASPSEE307 pKa = 4.02 PVQPEE312 pKa = 3.94 LRR314 pKa = 11.84 DD315 pKa = 3.52 ITHH318 pKa = 6.16 VSAARR323 pKa = 11.84 TFRR326 pKa = 11.84 GEE328 pKa = 3.87 SGRR331 pKa = 11.84 VGISRR336 pKa = 11.84 LGQKK340 pKa = 10.28 SSIQTRR346 pKa = 11.84 SGVTVGGRR354 pKa = 11.84 VHH356 pKa = 6.78 FRR358 pKa = 11.84 YY359 pKa = 10.18 SLSTIEE365 pKa = 5.63 DD366 pKa = 4.37 AIEE369 pKa = 4.1 DD370 pKa = 3.6 AGEE373 pKa = 4.2 IEE375 pKa = 4.23 LQVTNGSQGPSGSLQHH391 pKa = 5.73 TAEE394 pKa = 4.74 TILSEE399 pKa = 4.02 GHH401 pKa = 6.66 DD402 pKa = 3.81 AYY404 pKa = 11.65 VDD406 pKa = 3.29 VDD408 pKa = 3.6 MDD410 pKa = 4.51 SVGSLYY416 pKa = 10.93 SDD418 pKa = 3.2 IDD420 pKa = 4.59 LIDD423 pKa = 3.6 EE424 pKa = 4.6 HH425 pKa = 9.18 SEE427 pKa = 4.23 TPHH430 pKa = 6.79 GILVFHH436 pKa = 7.53 DD437 pKa = 3.81 EE438 pKa = 4.54 AEE440 pKa = 4.26 TDD442 pKa = 3.88 VVPVIDD448 pKa = 3.4 VSYY451 pKa = 10.73 VRR453 pKa = 11.84 KK454 pKa = 8.86 PLSTIPGSDD463 pKa = 3.37 LWPTNINIQNGPVDD477 pKa = 4.07 VDD479 pKa = 4.02 LQDD482 pKa = 4.64 SILPGIIITDD492 pKa = 3.59 SGVDD496 pKa = 3.19 GTYY499 pKa = 10.76 FLNTYY504 pKa = 8.03 LHH506 pKa = 6.92 PSLHH510 pKa = 5.77 KK511 pKa = 9.49 RR512 pKa = 11.84 KK513 pKa = 9.64 KK514 pKa = 10.03 RR515 pKa = 11.84 RR516 pKa = 11.84 FSS518 pKa = 3.23
Molecular weight: 56.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.79
IPC2_protein 4.774
IPC_protein 4.749
Toseland 4.635
ProMoST 4.876
Dawson 4.724
Bjellqvist 4.863
Wikipedia 4.609
Rodwell 4.622
Grimsley 4.546
Solomon 4.724
Lehninger 4.685
Nozaki 4.838
DTASelect 5.029
Thurlkill 4.635
EMBOSS 4.635
Sillero 4.902
Patrickios 3.961
IPC_peptide 4.736
IPC2_peptide 4.889
IPC2.peptide.svr19 4.869
Protein with the highest isoelectric point:
>sp|P50806|VL1_BPV5 Major capsid protein L1 OS=Bovine papillomavirus type 5 OX=40537 GN=L1 PE=3 SV=2
MM1 pKa = 7.42 KK2 pKa = 10.41 LVMLLRR8 pKa = 11.84 PPPQTHH14 pKa = 6.86 RR15 pKa = 11.84 ADD17 pKa = 3.83 TTDD20 pKa = 3.7 GLPGLQEE27 pKa = 4.02 EE28 pKa = 4.84 PRR30 pKa = 11.84 GGDD33 pKa = 3.38 GPTCSGPAPAIPDD46 pKa = 3.63 SPSRR50 pKa = 11.84 CLSRR54 pKa = 11.84 GFAGRR59 pKa = 11.84 DD60 pKa = 3.49 PGCHH64 pKa = 5.94 RR65 pKa = 11.84 NRR67 pKa = 11.84 HH68 pKa = 5.06 RR69 pKa = 11.84 VHH71 pKa = 7.06 PYY73 pKa = 9.33 ILSGGQRR80 pKa = 11.84 ILVTSSSSSTVQGPLSSGSSQHH102 pKa = 5.05 SQSRR106 pKa = 11.84 GRR108 pKa = 11.84 PPSPDD113 pKa = 2.95 STEE116 pKa = 4.0 TEE118 pKa = 4.12 RR119 pKa = 11.84 ARR121 pKa = 11.84 TPVNSDD127 pKa = 3.05 RR128 pKa = 11.84 QRR130 pKa = 11.84 AGEE133 pKa = 3.86 FDD135 pKa = 4.13 LLKK138 pKa = 10.79 GGCRR142 pKa = 11.84 PCCLIEE148 pKa = 4.36 GNGNKK153 pKa = 9.29 VKK155 pKa = 10.69 CLRR158 pKa = 11.84 FRR160 pKa = 11.84 LKK162 pKa = 10.15 KK163 pKa = 8.19 SHH165 pKa = 7.09 RR166 pKa = 11.84 SRR168 pKa = 11.84 FLDD171 pKa = 2.91 ITTTFWATGDD181 pKa = 3.83 EE182 pKa = 4.54 GSDD185 pKa = 3.23 RR186 pKa = 11.84 QGNGTILITFTDD198 pKa = 3.66 TTQRR202 pKa = 11.84 DD203 pKa = 3.79 LFLGSVSIPGEE214 pKa = 3.67 LSVRR218 pKa = 11.84 RR219 pKa = 11.84 ITISTDD225 pKa = 2.54
Molecular weight: 24.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.066
IPC2_protein 8.682
IPC_protein 8.77
Toseland 9.516
ProMoST 9.282
Dawson 9.721
Bjellqvist 9.648
Wikipedia 9.809
Rodwell 9.911
Grimsley 9.648
Solomon 9.867
Lehninger 9.853
Nozaki 9.999
DTASelect 9.487
Thurlkill 9.692
EMBOSS 9.97
Sillero 9.853
Patrickios 9.706
IPC_peptide 9.867
IPC2_peptide 9.092
IPC2.peptide.svr19 7.543
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2530
124
626
361.4
40.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.889 ± 0.678
2.609 ± 0.698
6.166 ± 0.303
5.178 ± 0.321
3.992 ± 0.4
7.51 ± 0.889
2.332 ± 0.325
4.111 ± 0.568
4.822 ± 0.877
8.735 ± 0.624
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.581 ± 0.338
3.913 ± 0.751
6.403 ± 0.79
4.743 ± 0.483
6.482 ± 0.836
8.656 ± 0.885
6.443 ± 0.796
6.126 ± 0.72
1.304 ± 0.277
3.004 ± 0.525
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here