Hubei tombus-like virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGE3|A0A1L3KGE3_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 17 OX=1923263 PE=4 SV=1
MM1 pKa = 7.33VSHH4 pKa = 6.95GNQVHH9 pKa = 6.59FNTSEE14 pKa = 4.27CVVIAPSIWTYY25 pKa = 7.26QVRR28 pKa = 11.84VHH30 pKa = 6.56AQCACNQYY38 pKa = 10.04EE39 pKa = 4.45SLIHH43 pKa = 6.27RR44 pKa = 11.84HH45 pKa = 5.34LASKK49 pKa = 10.46EE50 pKa = 3.67VDD52 pKa = 3.3LDD54 pKa = 3.23IGLWRR59 pKa = 11.84RR60 pKa = 11.84LADD63 pKa = 3.3RR64 pKa = 11.84WLHH67 pKa = 6.46IFQADD72 pKa = 3.78LAKK75 pKa = 10.82LSFKK79 pKa = 10.86DD80 pKa = 3.88VISHH84 pKa = 5.78FTGKK88 pKa = 9.9RR89 pKa = 11.84KK90 pKa = 9.66KK91 pKa = 9.77RR92 pKa = 11.84YY93 pKa = 8.96SSAFTHH99 pKa = 5.93YY100 pKa = 10.34CEE102 pKa = 5.32SGYY105 pKa = 9.34QSKK108 pKa = 10.67IKK110 pKa = 10.25MFIKK114 pKa = 9.84QDD116 pKa = 3.54KK117 pKa = 9.57YY118 pKa = 11.19PLDD121 pKa = 4.06TIYY124 pKa = 11.08DD125 pKa = 3.64KK126 pKa = 11.19SPRR129 pKa = 11.84AIQYY133 pKa = 8.55RR134 pKa = 11.84SPEE137 pKa = 3.87FNLAFMQYY145 pKa = 9.8IKK147 pKa = 10.44PIEE150 pKa = 4.12DD151 pKa = 2.73WVYY154 pKa = 11.32NNVTYY159 pKa = 10.71AVVSDD164 pKa = 3.77TPTIAKK170 pKa = 10.06AMNPYY175 pKa = 9.46QRR177 pKa = 11.84AEE179 pKa = 3.56IFRR182 pKa = 11.84YY183 pKa = 8.84KK184 pKa = 10.7VSWFKK189 pKa = 11.12NPKK192 pKa = 9.69FYY194 pKa = 10.87LIDD197 pKa = 3.45HH198 pKa = 6.64SAFDD202 pKa = 3.98SCISKK207 pKa = 10.0YY208 pKa = 10.14HH209 pKa = 6.74LKK211 pKa = 8.01TTHH214 pKa = 6.11KK215 pKa = 10.49KK216 pKa = 9.43YY217 pKa = 10.95FKK219 pKa = 10.75FFDD222 pKa = 3.3NFEE225 pKa = 3.99FRR227 pKa = 11.84KK228 pKa = 10.05LCQAQIHH235 pKa = 6.08NSGKK239 pKa = 7.35TRR241 pKa = 11.84SGIKK245 pKa = 10.29YY246 pKa = 8.88KK247 pKa = 11.05VEE249 pKa = 3.93GTRR252 pKa = 11.84MSGDD256 pKa = 3.54ADD258 pKa = 3.75TALGNCIVNLDD269 pKa = 4.58CITAVLCLSGISKK282 pKa = 10.42YY283 pKa = 11.1DD284 pKa = 3.24IMVDD288 pKa = 3.36GDD290 pKa = 3.86DD291 pKa = 4.37SIVIVEE297 pKa = 3.91QEE299 pKa = 4.0AVIEE303 pKa = 4.1EE304 pKa = 4.28SWFKK308 pKa = 11.14KK309 pKa = 10.43LGFITKK315 pKa = 10.02ISSTTNINEE324 pKa = 4.04VEE326 pKa = 4.44FCQSRR331 pKa = 11.84LCFDD335 pKa = 3.91GSRR338 pKa = 11.84YY339 pKa = 10.56AFVRR343 pKa = 11.84NPMRR347 pKa = 11.84MLAHH351 pKa = 6.43YY352 pKa = 8.19SICNKK357 pKa = 10.01KK358 pKa = 10.71YY359 pKa = 9.6SLKK362 pKa = 10.67QINHH366 pKa = 5.1WLKK369 pKa = 10.63GISMCEE375 pKa = 3.49NSLAGQYY382 pKa = 9.72PIYY385 pKa = 10.36NAIVKK390 pKa = 10.34AFDD393 pKa = 3.1VSDD396 pKa = 3.6RR397 pKa = 11.84FIIDD401 pKa = 3.39EE402 pKa = 4.12EE403 pKa = 4.63LEE405 pKa = 3.85QRR407 pKa = 11.84MKK409 pKa = 10.97GLSFSPLCRR418 pKa = 11.84SITTAARR425 pKa = 11.84KK426 pKa = 9.89SFEE429 pKa = 4.32EE430 pKa = 4.19VWGIDD435 pKa = 3.81HH436 pKa = 6.95ITQEE440 pKa = 4.76LIEE443 pKa = 4.91KK444 pKa = 10.21DD445 pKa = 2.86ILGFKK450 pKa = 10.46GFSKK454 pKa = 11.22VNGKK458 pKa = 9.7YY459 pKa = 10.19DD460 pKa = 3.84EE461 pKa = 5.27PIQRR465 pKa = 11.84AWQRR469 pKa = 11.84YY470 pKa = 4.85QLCHH474 pKa = 6.53EE475 pKa = 4.94SSSSCWRR482 pKa = 11.84DD483 pKa = 3.23CSEE486 pKa = 3.63GRR488 pKa = 11.84YY489 pKa = 10.05GSVGTVVDD497 pKa = 4.32PTDD500 pKa = 4.77DD501 pKa = 4.48PPDD504 pKa = 3.73TTTKK508 pKa = 10.4SLPNGTVGGCPARR521 pKa = 11.84KK522 pKa = 8.93GAAA525 pKa = 3.17

Molecular weight: 60.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFY9|A0A1L3KFY9_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 17 OX=1923263 PE=4 SV=1
MM1 pKa = 7.05MNQYY5 pKa = 10.18RR6 pKa = 11.84GRR8 pKa = 11.84GRR10 pKa = 11.84GTNFAMNLAAAAGGTAARR28 pKa = 11.84VAMDD32 pKa = 5.32RR33 pKa = 11.84LGQWWTQPTTPPTPQPNPYY52 pKa = 10.8QMGQLVAALPGRR64 pKa = 11.84GRR66 pKa = 11.84PRR68 pKa = 11.84RR69 pKa = 11.84GRR71 pKa = 11.84GRR73 pKa = 11.84GRR75 pKa = 11.84GRR77 pKa = 11.84GRR79 pKa = 11.84RR80 pKa = 11.84PQVARR85 pKa = 11.84SGGQPSSGVQTRR97 pKa = 11.84SGDD100 pKa = 3.6LFVARR105 pKa = 11.84GTEE108 pKa = 3.82VLGPVTGEE116 pKa = 3.91LQVLEE121 pKa = 5.06FNPSCDD127 pKa = 3.32GLPRR131 pKa = 11.84LAAVEE136 pKa = 4.14KK137 pKa = 9.17MYY139 pKa = 10.66HH140 pKa = 6.62RR141 pKa = 11.84YY142 pKa = 9.31RR143 pKa = 11.84IKK145 pKa = 10.58YY146 pKa = 8.83VNIAFKK152 pKa = 10.61SGSGTATAGNVAFGVCVGPKK172 pKa = 8.59VANVKK177 pKa = 7.87TQSDD181 pKa = 3.52IMKK184 pKa = 9.55LRR186 pKa = 11.84PFSYY190 pKa = 10.44VPAWKK195 pKa = 10.23NSSITVGSDD204 pKa = 2.39IDD206 pKa = 3.14IGRR209 pKa = 11.84FMISGSKK216 pKa = 10.39SEE218 pKa = 4.86DD219 pKa = 2.99GVAFTLYY226 pKa = 9.85IFASAAQLGVIQISYY241 pKa = 9.62EE242 pKa = 4.13VEE244 pKa = 4.35FSHH247 pKa = 6.51PTPFF251 pKa = 4.63

Molecular weight: 27.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1078

251

525

359.3

41.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.308 ± 1.035

2.505 ± 0.646

4.36 ± 1.033

5.009 ± 0.776

4.638 ± 0.424

6.215 ± 2.054

3.061 ± 0.779

6.215 ± 1.007

5.937 ± 1.287

7.05 ± 1.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.69 ± 0.468

4.267 ± 0.343

5.38 ± 1.273

4.917 ± 0.565

7.143 ± 1.199

7.05 ± 1.13

5.195 ± 0.249

6.308 ± 0.542

1.763 ± 0.178

3.989 ± 0.572
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski