Sea otter poxvirus
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U9QHV2|A0A2U9QHV2_9POXV Uncharacterized protein OS=Sea otter poxvirus OX=1416741 GN=SOPV-ELK-128 PE=4 SV=1
MM1 pKa = 7.27 TFIHH5 pKa = 6.86 EE6 pKa = 4.18 NDD8 pKa = 2.92 IHH10 pKa = 5.78 NTDD13 pKa = 4.4 KK14 pKa = 10.91 ISEE17 pKa = 4.01 QSQYY21 pKa = 10.84 DD22 pKa = 3.54 TMDD25 pKa = 3.2 VVEE28 pKa = 4.6 YY29 pKa = 10.86 GVIFPEE35 pKa = 5.55 LYY37 pKa = 10.63 NKK39 pKa = 10.43 LDD41 pKa = 3.38 TDD43 pKa = 4.37 ANDD46 pKa = 5.54 SIDD49 pKa = 4.93 DD50 pKa = 4.06 NNKK53 pKa = 9.08 PAAKK57 pKa = 10.38 ANIHH61 pKa = 5.86 EE62 pKa = 5.07 NIDD65 pKa = 3.66 NYY67 pKa = 11.51 NEE69 pKa = 4.19 TEE71 pKa = 4.23 DD72 pKa = 3.86 EE73 pKa = 4.2 NTNVKK78 pKa = 10.46 CSGIKK83 pKa = 9.96 KK84 pKa = 10.39 LNDD87 pKa = 2.97 NEE89 pKa = 4.47 YY90 pKa = 10.24 IVAIDD95 pKa = 3.24 ICSNDD100 pKa = 2.77 GTTNQTSSINGLVLYY115 pKa = 8.59 FAVTINGKK123 pKa = 8.28 NN124 pKa = 3.08
Molecular weight: 13.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.997
IPC2_protein 4.075
IPC_protein 4.037
Toseland 3.834
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.948
Rodwell 3.872
Grimsley 3.745
Solomon 4.012
Lehninger 3.973
Nozaki 4.139
DTASelect 4.368
Thurlkill 3.884
EMBOSS 3.961
Sillero 4.164
Patrickios 2.842
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.056
Protein with the highest isoelectric point:
>tr|A0A2U9QHV6|A0A2U9QHV6_9POXV IMV membrane protein OS=Sea otter poxvirus OX=1416741 GN=SOPV-ELK-107 PE=3 SV=1
MM1 pKa = 7.17 IRR3 pKa = 11.84 KK4 pKa = 7.44 NTTYY8 pKa = 10.76 NNKK11 pKa = 10.27 LIVSAFDD18 pKa = 3.25 IGVKK22 pKa = 10.43 NPARR26 pKa = 11.84 TVLEE30 pKa = 3.53 IHH32 pKa = 6.05 NNKK35 pKa = 8.64 VRR37 pKa = 11.84 ILDD40 pKa = 3.44 ITKK43 pKa = 10.34 LDD45 pKa = 3.48 WSSNWEE51 pKa = 3.56 QTIARR56 pKa = 11.84 DD57 pKa = 3.07 ITTYY61 pKa = 10.47 ISDD64 pKa = 3.28 IVLVEE69 pKa = 4.04 RR70 pKa = 11.84 QARR73 pKa = 11.84 SSPYY77 pKa = 9.32 IKK79 pKa = 9.95 FVYY82 pKa = 9.88 FIKK85 pKa = 10.73 GLLYY89 pKa = 10.04 NFPSKK94 pKa = 10.59 VYY96 pKa = 10.0 CVSPIMKK103 pKa = 9.88 GSTYY107 pKa = 10.14 RR108 pKa = 11.84 YY109 pKa = 8.4 RR110 pKa = 11.84 KK111 pKa = 8.83 HH112 pKa = 6.9 RR113 pKa = 11.84 SVKK116 pKa = 10.54 LFLSWLQIFGIYY128 pKa = 9.32 DD129 pKa = 3.28 QNISKK134 pKa = 10.74 KK135 pKa = 10.57 KK136 pKa = 10.15 LDD138 pKa = 4.02 DD139 pKa = 3.78 VADD142 pKa = 3.98 SFNMAMRR149 pKa = 11.84 YY150 pKa = 9.31 ILTKK154 pKa = 10.68
Molecular weight: 18.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.165
IPC2_protein 9.472
IPC_protein 9.472
Toseland 9.999
ProMoST 9.75
Dawson 10.218
Bjellqvist 9.897
Wikipedia 10.394
Rodwell 10.643
Grimsley 10.306
Solomon 10.248
Lehninger 10.204
Nozaki 9.984
DTASelect 9.897
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.131
Patrickios 10.116
IPC_peptide 10.233
IPC2_peptide 8.507
IPC2.peptide.svr19 8.449
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
130
0
130
39768
41
1286
305.9
35.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.815 ± 0.124
2.293 ± 0.145
6.229 ± 0.123
4.587 ± 0.12
4.657 ± 0.163
3.764 ± 0.15
2.484 ± 0.1
10.089 ± 0.173
6.857 ± 0.187
9.334 ± 0.2
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.099
6.714 ± 0.135
3.299 ± 0.117
2.764 ± 0.096
4.277 ± 0.113
7.825 ± 0.123
6.759 ± 0.133
6.05 ± 0.146
0.631 ± 0.048
4.906 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here