Gordonia phage Pleakley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345M6K7|A0A345M6K7_9CAUD Uncharacterized protein OS=Gordonia phage Pleakley OX=2283246 GN=89 PE=4 SV=1
MM1 pKa = 7.87TYY3 pKa = 9.69TPAPGDD9 pKa = 3.75VITSRR14 pKa = 11.84TLDD17 pKa = 3.7PVDD20 pKa = 4.05GFDD23 pKa = 4.52LIAYY27 pKa = 7.38EE28 pKa = 5.06VYY30 pKa = 9.49DD31 pKa = 4.15TDD33 pKa = 3.57SSPIGEE39 pKa = 4.07EE40 pKa = 4.35TEE42 pKa = 4.58NGGCYY47 pKa = 10.11DD48 pKa = 4.0RR49 pKa = 11.84ADD51 pKa = 3.33IEE53 pKa = 4.03AWRR56 pKa = 11.84NDD58 pKa = 2.61EE59 pKa = 3.94WYY61 pKa = 8.49YY62 pKa = 11.35AGVVVVASRR71 pKa = 11.84AGIPLGSGAIWGCDD85 pKa = 2.81TGDD88 pKa = 3.1YY89 pKa = 9.63WDD91 pKa = 5.22RR92 pKa = 11.84GDD94 pKa = 3.47GTYY97 pKa = 10.46SEE99 pKa = 5.09LLDD102 pKa = 3.65VTTRR106 pKa = 11.84PAGTYY111 pKa = 7.07QTLSGPVARR120 pKa = 11.84PAGVEE125 pKa = 4.06FAHH128 pKa = 7.22GYY130 pKa = 10.62GDD132 pKa = 4.51DD133 pKa = 5.16LIAEE137 pKa = 4.39ALIEE141 pKa = 4.26ARR143 pKa = 11.84ATLARR148 pKa = 11.84LGVSAA153 pKa = 5.4

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345M6D0|A0A345M6D0_9CAUD Uncharacterized protein OS=Gordonia phage Pleakley OX=2283246 GN=12 PE=4 SV=1
MM1 pKa = 7.48TLMPSSPGSKK11 pKa = 9.75KK12 pKa = 10.37GLSRR16 pKa = 11.84LGRR19 pKa = 11.84GGTKK23 pKa = 9.68EE24 pKa = 3.69YY25 pKa = 10.51RR26 pKa = 11.84DD27 pKa = 3.73LVRR30 pKa = 11.84KK31 pKa = 10.06IEE33 pKa = 4.08AAGGEE38 pKa = 4.31VRR40 pKa = 11.84KK41 pKa = 8.61PTGKK45 pKa = 10.04GHH47 pKa = 6.46PKK49 pKa = 9.84VYY51 pKa = 10.43LGGRR55 pKa = 11.84FIMTLCCTPSDD66 pKa = 3.29HH67 pKa = 8.02RR68 pKa = 11.84SRR70 pKa = 11.84KK71 pKa = 9.67NEE73 pKa = 3.68LARR76 pKa = 11.84LRR78 pKa = 11.84RR79 pKa = 11.84AGLEE83 pKa = 3.76ISS85 pKa = 3.56

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

20220

30

1847

210.6

23.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.98 ± 0.41

0.781 ± 0.111

7.389 ± 0.26

6.607 ± 0.331

3.165 ± 0.158

8.867 ± 0.389

1.835 ± 0.149

4.787 ± 0.159

4.52 ± 0.225

7.72 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.25 ± 0.108

3.388 ± 0.145

5.134 ± 0.154

3.21 ± 0.16

6.949 ± 0.353

5.376 ± 0.175

6.251 ± 0.221

7.171 ± 0.215

1.963 ± 0.132

2.656 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski