Streptococcus satellite phage Javan280
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIW3|A0A4D5ZIW3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan280 OX=2558603 GN=JavanS280_0003 PE=4 SV=1
MM1 pKa = 7.26 TNKK4 pKa = 10.03 NNSFDD9 pKa = 4.16 LANEE13 pKa = 3.81 RR14 pKa = 11.84 AKK16 pKa = 11.21 NFGVWLEE23 pKa = 4.03 EE24 pKa = 4.51 AYY26 pKa = 8.85 QTMLDD31 pKa = 4.09 FSLEE35 pKa = 4.17 NKK37 pKa = 9.58 FDD39 pKa = 3.93 CYY41 pKa = 10.78 NAEE44 pKa = 4.0 EE45 pKa = 4.19 QKK47 pKa = 10.67 QLEE50 pKa = 4.41 QVLEE54 pKa = 4.12 TLMDD58 pKa = 4.15 FCDD61 pKa = 3.49 MWEE64 pKa = 4.09 KK65 pKa = 10.9 GQIILVSEE73 pKa = 4.12 EE74 pKa = 4.36 RR75 pKa = 11.84 EE76 pKa = 3.83 MSKK79 pKa = 11.0
Molecular weight: 9.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.438
IPC2_protein 4.342
IPC_protein 4.202
Toseland 4.05
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 3.999
Rodwell 4.05
Grimsley 3.973
Solomon 4.139
Lehninger 4.088
Nozaki 4.253
DTASelect 4.355
Thurlkill 4.062
EMBOSS 4.024
Sillero 4.317
Patrickios 3.783
IPC_peptide 4.139
IPC2_peptide 4.304
IPC2.peptide.svr19 4.225
Protein with the highest isoelectric point:
>tr|A0A4D5ZN24|A0A4D5ZN24_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan280 OX=2558603 GN=JavanS280_0015 PE=4 SV=1
MM1 pKa = 7.28 VFDD4 pKa = 4.0 MRR6 pKa = 11.84 QHH8 pKa = 6.19 RR9 pKa = 11.84 RR10 pKa = 11.84 FLNFPTLGNLVVVDD24 pKa = 5.03 DD25 pKa = 4.83 LLSSEE30 pKa = 4.15 RR31 pKa = 11.84 TPRR34 pKa = 11.84 KK35 pKa = 8.49 YY36 pKa = 9.19 TIKK39 pKa = 10.36 II40 pKa = 3.53
Molecular weight: 4.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.472
IPC_protein 10.116
Toseland 10.452
ProMoST 10.101
Dawson 10.57
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 10.804
Grimsley 10.628
Solomon 10.687
Lehninger 10.657
Nozaki 10.409
DTASelect 10.248
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.482
Patrickios 10.716
IPC_peptide 10.687
IPC2_peptide 8.931
IPC2.peptide.svr19 8.7
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
3610
40
381
150.4
17.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.014 ± 0.343
0.72 ± 0.154
5.485 ± 0.422
7.839 ± 0.656
4.155 ± 0.298
4.294 ± 0.365
2.022 ± 0.311
8.421 ± 0.421
9.335 ± 0.458
10.471 ± 0.691
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.853 ± 0.274
5.485 ± 0.466
2.355 ± 0.243
4.571 ± 0.35
4.266 ± 0.385
6.122 ± 0.314
6.205 ± 0.452
4.986 ± 0.368
0.942 ± 0.185
4.46 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here