Streptomyces phage Celia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; unclassified Arquatrovirinae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KRA4|A0A516KRA4_9CAUD Uncharacterized protein OS=Streptomyces phage Celia OX=2590946 GN=21 PE=4 SV=1
MM1 pKa = 7.76ALNDD5 pKa = 4.23DD6 pKa = 3.85ATLVIGSGNYY16 pKa = 7.78LTAPAGTPIPEE27 pKa = 4.97DD28 pKa = 3.74LLSPASPWEE37 pKa = 4.29AVGHH41 pKa = 5.85TSLEE45 pKa = 4.53DD46 pKa = 3.06IFGITSEE53 pKa = 4.22GGEE56 pKa = 3.92ATVIGTLQNKK66 pKa = 7.42SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.2TMTFTLQQFDD87 pKa = 3.87APGLRR92 pKa = 11.84LYY94 pKa = 10.82YY95 pKa = 10.24GANAPTLPDD104 pKa = 3.54GSIGVPTEE112 pKa = 4.05PEE114 pKa = 3.85PTVCAFLAIFVDD126 pKa = 4.23GDD128 pKa = 3.48NHH130 pKa = 6.56FAFYY134 pKa = 10.71APKK137 pKa = 10.65AEE139 pKa = 4.56IYY141 pKa = 10.28RR142 pKa = 11.84ADD144 pKa = 4.92DD145 pKa = 3.37MTLSDD150 pKa = 4.12TEE152 pKa = 4.4SLAGLPLGVKK162 pKa = 10.09PMAYY166 pKa = 10.07SSNPWTYY173 pKa = 11.07AVTPLGSSIAATGATAGIPGAFTPTGATPPADD205 pKa = 3.95LEE207 pKa = 4.22ALQGSTIVASPGTAWTEE224 pKa = 4.04GQYY227 pKa = 10.81VALGDD232 pKa = 3.83LTSAHH237 pKa = 7.06WDD239 pKa = 3.3GDD241 pKa = 3.61SWEE244 pKa = 4.41TGAAPAA250 pKa = 4.82

Molecular weight: 25.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KR90|A0A516KR90_9CAUD Uncharacterized protein OS=Streptomyces phage Celia OX=2590946 GN=8 PE=4 SV=1
MM1 pKa = 7.7LPDD4 pKa = 3.52QPKK7 pKa = 10.54GPTLRR12 pKa = 11.84IVTWNAGNGTAADD25 pKa = 4.1LRR27 pKa = 11.84ALLARR32 pKa = 11.84CDD34 pKa = 3.81VLAGQEE40 pKa = 3.83WGDD43 pKa = 3.62RR44 pKa = 11.84ADD46 pKa = 3.62LSRR49 pKa = 11.84AARR52 pKa = 11.84LMDD55 pKa = 3.11WTVLDD60 pKa = 3.65GRR62 pKa = 11.84GAAGQASTPLLVGPHH77 pKa = 4.07VRR79 pKa = 11.84VRR81 pKa = 11.84RR82 pKa = 11.84EE83 pKa = 3.41VAVPVLRR90 pKa = 11.84SRR92 pKa = 11.84YY93 pKa = 8.33IGPGAGPDD101 pKa = 3.49HH102 pKa = 7.09NKK104 pKa = 9.48PKK106 pKa = 10.27HH107 pKa = 6.08AIGGLLSLDD116 pKa = 3.22GSTFGVVSTHH126 pKa = 6.9LPATQGRR133 pKa = 11.84SLRR136 pKa = 11.84HH137 pKa = 5.48EE138 pKa = 4.12AAEE141 pKa = 4.14DD142 pKa = 3.62QVDD145 pKa = 3.72EE146 pKa = 4.29LVRR149 pKa = 11.84RR150 pKa = 11.84FNRR153 pKa = 11.84RR154 pKa = 11.84QFPWFLCGDD163 pKa = 4.27FNAVPTSGALSPLYY177 pKa = 10.38RR178 pKa = 11.84SGWTNSHH185 pKa = 6.32RR186 pKa = 11.84ASRR189 pKa = 11.84PLATHH194 pKa = 5.73GQRR197 pKa = 11.84PIDD200 pKa = 3.88YY201 pKa = 8.18VWWDD205 pKa = 3.16KK206 pKa = 10.99GHH208 pKa = 5.91GVRR211 pKa = 11.84FVRR214 pKa = 11.84HH215 pKa = 4.49AVEE218 pKa = 4.42RR219 pKa = 11.84TRR221 pKa = 11.84SDD223 pKa = 2.6HH224 pKa = 6.45RR225 pKa = 11.84ALVAQFKK232 pKa = 9.02LTT234 pKa = 3.44

Molecular weight: 25.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

15154

43

1161

196.8

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.126 ± 0.345

0.732 ± 0.102

6.949 ± 0.317

6.975 ± 0.435

2.871 ± 0.162

8.585 ± 0.265

2.059 ± 0.178

4.164 ± 0.226

3.854 ± 0.22

8.4 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.129

2.791 ± 0.15

4.976 ± 0.256

3.174 ± 0.138

7.054 ± 0.5

5.939 ± 0.286

6.408 ± 0.277

7.16 ± 0.244

1.927 ± 0.139

2.778 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski