Pseudonaja textilis (Eastern brown snake)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27547 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A670YCV2|A0A670YCV2_PSETE Ribosomal protein S6 kinase OS=Pseudonaja textilis OX=8673 GN=RPS6KA3 PE=3 SV=1
WW1 pKa = 7.31ILLFLIFLSSFDD13 pKa = 4.42LDD15 pKa = 3.52LSYY18 pKa = 11.09FLIFWDD24 pKa = 4.4GNGQASYY31 pKa = 10.95SVQEE35 pKa = 4.18EE36 pKa = 4.21PLYY39 pKa = 11.01DD40 pKa = 4.04PSLHH44 pKa = 6.33HH45 pKa = 6.2VAPLTLIHH53 pKa = 6.67LKK55 pKa = 10.67AVTEE59 pKa = 4.53DD60 pKa = 3.7GQFEE64 pKa = 4.47DD65 pKa = 5.6AVDD68 pKa = 3.28

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A670XR62|A0A670XR62_PSETE Dynein light chain OS=Pseudonaja textilis OX=8673 GN=DNAL4 PE=3 SV=1
MM1 pKa = 7.18GRR3 pKa = 11.84KK4 pKa = 7.98LTSPRR9 pKa = 11.84LRR11 pKa = 11.84PKK13 pKa = 10.3SIRR16 pKa = 11.84GAGLSSTSFTPASARR31 pKa = 11.84NMKK34 pKa = 9.8GAPNWASPGG43 pKa = 3.54

Molecular weight: 4.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18073

9474

27547

16957327

11

8747

615.6

69.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.288 ± 0.013

2.283 ± 0.013

4.855 ± 0.01

7.083 ± 0.018

4.029 ± 0.011

5.942 ± 0.016

2.609 ± 0.006

5.233 ± 0.012

6.294 ± 0.017

10.037 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.006

4.227 ± 0.009

5.397 ± 0.017

4.724 ± 0.015

5.167 ± 0.011

8.181 ± 0.018

5.267 ± 0.009

5.939 ± 0.011

1.219 ± 0.005

2.945 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski