Xylella phage Cota
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F8ZKP3|A0A6F8ZKP3_9CAUD DNA polymerase I phage-associated OS=Xylella phage Cota OX=2699877 PE=4 SV=1
MM1 pKa = 7.94 AINDD5 pKa = 3.81 PNYY8 pKa = 10.58 RR9 pKa = 11.84 YY10 pKa = 8.06 ATQTWEE16 pKa = 3.8 ADD18 pKa = 3.74 GVRR21 pKa = 11.84 TQYY24 pKa = 11.31 DD25 pKa = 3.22 IAFDD29 pKa = 3.37 GGYY32 pKa = 9.5 IRR34 pKa = 11.84 QSDD37 pKa = 4.05 VVAFSVLVDD46 pKa = 3.8 EE47 pKa = 4.97 DD48 pKa = 3.9 TGLTTDD54 pKa = 3.71 RR55 pKa = 11.84 QIHH58 pKa = 5.38 PVVFLSEE65 pKa = 4.41 SVDD68 pKa = 4.7 PEE70 pKa = 4.7 TEE72 pKa = 3.57 WKK74 pKa = 7.94 TAQVQITPAVPAGRR88 pKa = 11.84 RR89 pKa = 11.84 VVIFRR94 pKa = 11.84 STEE97 pKa = 3.37 KK98 pKa = 10.68 SAALVNYY105 pKa = 8.39 TNGSILTEE113 pKa = 4.18 KK114 pKa = 10.89 NLDD117 pKa = 3.68 LANDD121 pKa = 3.48 QSVFGIAEE129 pKa = 3.86 IMDD132 pKa = 4.19 GLNAARR138 pKa = 11.84 IDD140 pKa = 3.7 INSQVQEE147 pKa = 4.4 VIDD150 pKa = 4.1 MNQLIQEE157 pKa = 4.57 LYY159 pKa = 8.82 EE160 pKa = 4.14 QIIEE164 pKa = 4.07 LLAAGGIVSVAPRR177 pKa = 11.84 VWFGTGNGEE186 pKa = 4.1 DD187 pKa = 3.42 TDD189 pKa = 4.38 FPLVGADD196 pKa = 3.21 VDD198 pKa = 4.05 GSGFYY203 pKa = 9.44 DD204 pKa = 3.89 TYY206 pKa = 10.75 IGGVGMEE213 pKa = 4.85 PDD215 pKa = 3.15 TDD217 pKa = 3.7 YY218 pKa = 11.56 AIIMGEE224 pKa = 4.4 TIADD228 pKa = 3.52 TLIRR232 pKa = 11.84 FTVPPADD239 pKa = 3.32 GVRR242 pKa = 11.84 WFTVLRR248 pKa = 11.84 GYY250 pKa = 10.57 AKK252 pKa = 10.06 PYY254 pKa = 9.3 TGPQPVVSLSMPVIDD269 pKa = 5.71 VPDD272 pKa = 3.88 PQYY275 pKa = 10.99 FIGKK279 pKa = 7.94 EE280 pKa = 3.8 SEE282 pKa = 4.36 YY283 pKa = 11.54 ALLRR287 pKa = 11.84 ALNDD291 pKa = 3.43 TEE293 pKa = 4.01 ATFFVKK299 pKa = 10.38 EE300 pKa = 4.16 IPISGDD306 pKa = 3.27 PANKK310 pKa = 10.23 LGDD313 pKa = 3.61 GSYY316 pKa = 11.42 VSITQRR322 pKa = 11.84 GAAQVVLEE330 pKa = 4.59 PDD332 pKa = 3.29 TAEE335 pKa = 3.96 VQLIVPAGYY344 pKa = 10.41 LPATRR349 pKa = 11.84 ALNSVISITCEE360 pKa = 3.9 YY361 pKa = 10.79 GDD363 pKa = 3.7 GNAWIVSGDD372 pKa = 3.62 LAKK375 pKa = 10.53 EE376 pKa = 3.88
Molecular weight: 40.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.137
IPC2_protein 3.999
IPC_protein 3.999
Toseland 3.783
ProMoST 4.126
Dawson 3.973
Bjellqvist 4.139
Wikipedia 3.884
Rodwell 3.821
Grimsley 3.694
Solomon 3.973
Lehninger 3.923
Nozaki 4.075
DTASelect 4.304
Thurlkill 3.821
EMBOSS 3.897
Sillero 4.113
Patrickios 3.363
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 4.028
Protein with the highest isoelectric point:
>tr|A0A6F8ZKJ8|A0A6F8ZKJ8_9CAUD Uncharacterized protein OS=Xylella phage Cota OX=2699877 PE=4 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 NLINRR8 pKa = 11.84 LLRR11 pKa = 11.84 PLLAQRR17 pKa = 11.84 YY18 pKa = 6.97 IVRR21 pKa = 11.84 LEE23 pKa = 3.95 PFVLADD29 pKa = 3.67 LRR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 10.16 VGGTCIVTSQTTEE46 pKa = 3.45 HH47 pKa = 6.09 MAGYY51 pKa = 9.74 QLGIAHH57 pKa = 6.71 VLNVLQEE64 pKa = 4.62 GYY66 pKa = 8.49 TVSRR70 pKa = 11.84 SS71 pKa = 3.24
Molecular weight: 8.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.794
IPC_protein 10.789
Toseland 10.277
ProMoST 10.292
Dawson 10.54
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 10.496
Grimsley 10.657
Solomon 10.672
Lehninger 10.613
Nozaki 10.321
DTASelect 10.379
Thurlkill 10.379
EMBOSS 10.73
Sillero 10.482
Patrickios 10.248
IPC_peptide 10.657
IPC2_peptide 9.677
IPC2.peptide.svr19 8.17
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
12465
44
1490
244.4
27.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.413 ± 0.575
0.85 ± 0.142
6.378 ± 0.231
6.45 ± 0.297
3.506 ± 0.179
7.942 ± 0.375
1.821 ± 0.209
4.822 ± 0.226
4.765 ± 0.318
7.95 ± 0.247
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.688 ± 0.169
4.099 ± 0.185
4.108 ± 0.178
4.3 ± 0.294
6.611 ± 0.302
5.552 ± 0.275
6.081 ± 0.287
6.611 ± 0.315
1.621 ± 0.114
3.434 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here