Streptomyces sp. HG99

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5ISE8|A0A2G5ISE8_9ACTN Isochorismate synthase OS=Streptomyces sp. HG99 OX=1958787 GN=B1C81_20285 PE=3 SV=1
MM1 pKa = 7.76PMTARR6 pKa = 11.84DD7 pKa = 3.98YY8 pKa = 11.65AWLFTPGSTFTYY20 pKa = 10.02EE21 pKa = 3.98SGDD24 pKa = 3.36TGVIHH29 pKa = 6.28VADD32 pKa = 4.93GGEE35 pKa = 3.77LWLPTGHH42 pKa = 6.73VVACDD47 pKa = 3.45PFVCLGRR54 pKa = 11.84GDD56 pKa = 4.33NEE58 pKa = 4.29PFTVAVEE65 pKa = 3.97PGRR68 pKa = 11.84YY69 pKa = 8.58RR70 pKa = 11.84VEE72 pKa = 3.84AAVATLTRR80 pKa = 11.84PGEE83 pKa = 4.26APSGDD88 pKa = 3.15PHH90 pKa = 8.32LRR92 pKa = 11.84VAAARR97 pKa = 11.84LVIRR101 pKa = 11.84DD102 pKa = 3.78APTATWEE109 pKa = 4.19LAVQPGQDD117 pKa = 3.68LAEE120 pKa = 4.78LGDD123 pKa = 3.93DD124 pKa = 3.44EE125 pKa = 5.56FYY127 pKa = 10.87GYY129 pKa = 11.02GVDD132 pKa = 3.41AGTGAFYY139 pKa = 10.91DD140 pKa = 3.9AAADD144 pKa = 3.64ASFPEE149 pKa = 4.59CEE151 pKa = 4.26GDD153 pKa = 5.7EE154 pKa = 4.6GPLWDD159 pKa = 4.84AFEE162 pKa = 4.32NSAWAPGPHH171 pKa = 6.81LVTAPDD177 pKa = 3.45TGHH180 pKa = 6.55NLVAFSSGWGDD191 pKa = 3.14GAYY194 pKa = 6.86PTWIGRR200 pKa = 11.84TASGEE205 pKa = 4.25VTCFVTDD212 pKa = 3.69FFVAPDD218 pKa = 4.28PSDD221 pKa = 4.43DD222 pKa = 3.81PAA224 pKa = 6.26

Molecular weight: 23.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5ITJ7|A0A2G5ITJ7_9ACTN Uncharacterized protein OS=Streptomyces sp. HG99 OX=1958787 GN=B1C81_18080 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7341

0

7341

2323136

18

2614

316.5

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.214 ± 0.04

0.798 ± 0.009

6.089 ± 0.023

5.88 ± 0.03

2.712 ± 0.017

9.146 ± 0.028

2.348 ± 0.013

3.376 ± 0.018

2.431 ± 0.023

10.112 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.787 ± 0.012

1.88 ± 0.016

6.051 ± 0.027

3.006 ± 0.018

7.889 ± 0.037

5.209 ± 0.024

6.121 ± 0.029

8.247 ± 0.028

1.545 ± 0.011

2.158 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski