Marinobacter sp. Z-D5-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; unclassified Marinobacter

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4CLF5|A0A2T4CLF5_9ALTE Chaperone protein ClpB OS=Marinobacter sp. Z-D5-3 OX=2137200 GN=clpB PE=3 SV=1
MM1 pKa = 7.37FKK3 pKa = 9.48KK4 pKa = 9.12TLISLAVASSVGLTGCFDD22 pKa = 3.72SGEE25 pKa = 4.29TGANANPQYY34 pKa = 10.96NIVDD38 pKa = 3.77TTIDD42 pKa = 3.34RR43 pKa = 11.84SIVRR47 pKa = 11.84PVFDD51 pKa = 5.77PNPISPTVGFPANFDD66 pKa = 4.02LLLLLGATQSSNYY79 pKa = 10.03DD80 pKa = 3.35FTGLTSGTDD89 pKa = 3.5PASNAINDD97 pKa = 4.01LAGFSTSGQFNIAFDD112 pKa = 4.1GSLNPDD118 pKa = 3.66TVIKK122 pKa = 9.97GQTVFLVPLNVAPAVDD138 pKa = 4.38SAPLALPSVNPASVTGVDD156 pKa = 3.77TTPEE160 pKa = 3.83NQPNYY165 pKa = 8.51RR166 pKa = 11.84VEE168 pKa = 5.08VITQDD173 pKa = 2.98GVTNNTIRR181 pKa = 11.84ITPLEE186 pKa = 4.26PLLEE190 pKa = 4.29SKK192 pKa = 10.47KK193 pKa = 10.68YY194 pKa = 10.19IVIVSDD200 pKa = 3.79GVTGANGKK208 pKa = 7.82PTEE211 pKa = 4.03QSIAAQQLTTGPLGSEE227 pKa = 3.86NLSNIRR233 pKa = 11.84DD234 pKa = 3.75LVNALNGLGQQIISATGKK252 pKa = 10.21DD253 pKa = 3.17VALAYY258 pKa = 9.05TFTTAGQSTVLKK270 pKa = 10.82NMASPGTYY278 pKa = 9.91LQALGQKK285 pKa = 9.61IGFTALLKK293 pKa = 10.51AVRR296 pKa = 11.84DD297 pKa = 3.98NNPNANFSQLTEE309 pKa = 3.96ILGEE313 pKa = 4.0IQDD316 pKa = 4.57GNVEE320 pKa = 4.76DD321 pKa = 4.5YY322 pKa = 10.85PEE324 pKa = 3.85TAAAVQEE331 pKa = 4.26AASVATSTNIQNAIGASFSTGALHH355 pKa = 7.45FPTTRR360 pKa = 11.84PSFFYY365 pKa = 11.06SKK367 pKa = 10.43DD368 pKa = 3.35APATGLATIANLPDD382 pKa = 3.3GNAIKK387 pKa = 10.49SAAAGVSVSQGSIVLPYY404 pKa = 8.53YY405 pKa = 10.33QPIPAVSGGDD415 pKa = 3.46ALVSRR420 pKa = 11.84GWQGDD425 pKa = 3.95TNLEE429 pKa = 3.92TSLNSNLPASGTTFQFLRR447 pKa = 11.84DD448 pKa = 3.12IDD450 pKa = 3.6GTLNVNSNFPYY461 pKa = 10.28PKK463 pKa = 10.09VEE465 pKa = 4.16GNVAVPVVAFYY476 pKa = 10.67PNAGICGGGTIAGVTILQHH495 pKa = 6.75GITVDD500 pKa = 3.54RR501 pKa = 11.84SVSMLPGILIAASTCQAVIAIDD523 pKa = 3.78QPLHH527 pKa = 5.78GLAGATAGTLPGLDD541 pKa = 3.3VLTAEE546 pKa = 4.81DD547 pKa = 3.71VGTAFDD553 pKa = 4.29SVPGLAYY560 pKa = 9.62IGEE563 pKa = 4.18RR564 pKa = 11.84HH565 pKa = 6.13FNYY568 pKa = 10.06TDD570 pKa = 3.56AGSLTPVQAASIADD584 pKa = 3.73VEE586 pKa = 4.68SGSLYY591 pKa = 11.26VNLKK595 pKa = 9.57SLQGARR601 pKa = 11.84DD602 pKa = 3.72NNRR605 pKa = 11.84QGVLDD610 pKa = 4.72LLNLSASVDD619 pKa = 3.6GSTSPQGISGLDD631 pKa = 3.1IRR633 pKa = 11.84GDD635 pKa = 3.71ANPDD639 pKa = 3.41FTSTTPVSFIGHH651 pKa = 5.68SLGGITGTTFASLSNDD667 pKa = 3.31PAVRR671 pKa = 11.84GAYY674 pKa = 8.27AAPGLPPIQFPEE686 pKa = 4.2LEE688 pKa = 4.22NVSLHH693 pKa = 5.1NTGGQIAKK701 pKa = 10.03LVEE704 pKa = 4.07NSQSISSQVLPALPQQGTSDD724 pKa = 4.5LEE726 pKa = 4.06TFLYY730 pKa = 10.52VFQSVLDD737 pKa = 3.85EE738 pKa = 4.33VDD740 pKa = 3.06PVTYY744 pKa = 10.29AKK746 pKa = 10.54SLGASEE752 pKa = 4.52TNLLVTEE759 pKa = 4.63VIGDD763 pKa = 3.6STVPNEE769 pKa = 4.35ANVNPLGSALSAPLAGTEE787 pKa = 3.87PLMALFDD794 pKa = 4.46LGAGGTNLADD804 pKa = 3.47GTALNVVDD812 pKa = 5.43SNTAPTADD820 pKa = 3.82AGLPAAVFFAGSNPCAQANHH840 pKa = 5.63GTFVVPQAPNDD851 pKa = 3.59QCPGGIANTIDD862 pKa = 3.51AFSAMVTQTAQAIAMEE878 pKa = 4.27QLPVDD883 pKa = 3.88AAIQATLGQSPTIDD897 pKa = 3.24STLDD901 pKa = 3.13QDD903 pKa = 3.79QQ904 pKa = 3.43

Molecular weight: 92.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4CLE1|A0A2T4CLE1_9ALTE Crp/Fnr family transcriptional regulator OS=Marinobacter sp. Z-D5-3 OX=2137200 GN=C9984_00050 PE=4 SV=1
MM1 pKa = 7.57ANSPQAKK8 pKa = 9.2KK9 pKa = 10.21RR10 pKa = 11.84ARR12 pKa = 11.84QNEE15 pKa = 4.12KK16 pKa = 10.14NRR18 pKa = 11.84KK19 pKa = 8.17HH20 pKa = 5.45NASLRR25 pKa = 11.84SMARR29 pKa = 11.84TYY31 pKa = 10.76VKK33 pKa = 10.39KK34 pKa = 10.26IQSKK38 pKa = 10.01IEE40 pKa = 3.9AGNYY44 pKa = 9.62EE45 pKa = 4.01EE46 pKa = 5.09AQAAFQQAQPILDD59 pKa = 3.6SMVNKK64 pKa = 10.8GIFAKK69 pKa = 10.82NKK71 pKa = 7.26VARR74 pKa = 11.84SKK76 pKa = 11.12SRR78 pKa = 11.84LSAKK82 pKa = 9.95IKK84 pKa = 10.18ALKK87 pKa = 9.95SAA89 pKa = 4.08

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

58420

28

1159

290.6

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.949 ± 0.186

0.828 ± 0.045

5.565 ± 0.121

6.768 ± 0.193

3.826 ± 0.12

8.025 ± 0.168

1.953 ± 0.089

5.404 ± 0.12

3.566 ± 0.155

10.765 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.491 ± 0.105

3.487 ± 0.148

4.575 ± 0.112

4.06 ± 0.139

6.301 ± 0.161

5.847 ± 0.175

5.262 ± 0.139

7.432 ± 0.14

1.392 ± 0.075

2.504 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski