bacterium C-53

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9C100|A0A3A9C100_9BACT LysM peptidoglycan-binding domain-containing protein OS=bacterium C-53 OX=2320109 GN=D7X88_11095 PE=4 SV=1
MM1 pKa = 7.36SMSVSKK7 pKa = 10.85KK8 pKa = 10.57DD9 pKa = 3.61LLLLLGLCGVLAAALVYY26 pKa = 9.05FFVYY30 pKa = 10.1QPFMQKK36 pKa = 9.81SDD38 pKa = 3.53ALEE41 pKa = 4.25TEE43 pKa = 4.33NNSLSVRR50 pKa = 11.84IAEE53 pKa = 4.25LEE55 pKa = 3.79AMEE58 pKa = 4.84AKK60 pKa = 10.11RR61 pKa = 11.84EE62 pKa = 3.97EE63 pKa = 4.89YY64 pKa = 9.11ITEE67 pKa = 4.1TEE69 pKa = 4.09DD70 pKa = 3.04MNRR73 pKa = 11.84QIASVYY79 pKa = 10.82NLFPSNVMAEE89 pKa = 4.09DD90 pKa = 4.89AIYY93 pKa = 10.21LAIYY97 pKa = 9.62EE98 pKa = 4.32EE99 pKa = 4.66SLAPLQVNGITIEE112 pKa = 4.07PQEE115 pKa = 3.95VQYY118 pKa = 11.34QSGGNEE124 pKa = 3.88DD125 pKa = 3.88TEE127 pKa = 4.38GTEE130 pKa = 4.21EE131 pKa = 5.35KK132 pKa = 10.62EE133 pKa = 4.53DD134 pKa = 4.87AIDD137 pKa = 3.8KK138 pKa = 10.62AKK140 pKa = 10.86SDD142 pKa = 3.81APADD146 pKa = 3.89QAALEE151 pKa = 4.43ADD153 pKa = 3.67AEE155 pKa = 4.48LEE157 pKa = 4.23GTAVDD162 pKa = 4.94SDD164 pKa = 4.44ADD166 pKa = 3.77DD167 pKa = 3.76EE168 pKa = 4.35AAEE171 pKa = 5.21DD172 pKa = 4.65RR173 pKa = 11.84ITDD176 pKa = 3.62AGANMALYY184 pKa = 8.06NQPVTISYY192 pKa = 9.07EE193 pKa = 3.76LSYY196 pKa = 11.37DD197 pKa = 3.58GLKK200 pKa = 10.41RR201 pKa = 11.84CINYY205 pKa = 9.38ICTNPNRR212 pKa = 11.84MTVEE216 pKa = 4.38SVAAAYY222 pKa = 10.04DD223 pKa = 3.71NTSGMLSGNMRR234 pKa = 11.84VNMYY238 pKa = 10.69YY239 pKa = 9.45LTGNGKK245 pKa = 9.6PYY247 pKa = 10.06TPPDD251 pKa = 3.86FSNVLIGNTNLFGSIVLPEE270 pKa = 4.12TQSDD274 pKa = 3.69SGEE277 pKa = 4.09DD278 pKa = 3.48AEE280 pKa = 5.67EE281 pKa = 4.25EE282 pKa = 3.97NTDD285 pKa = 4.49ANEE288 pKa = 3.87QMM290 pKa = 4.49

Molecular weight: 31.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9CHT8|A0A3A9CHT8_9BACT ROK family protein OS=bacterium C-53 OX=2320109 GN=D7X88_04840 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3732

0

3732

1175357

22

2617

314.9

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.27 ± 0.043

1.531 ± 0.015

5.592 ± 0.032

7.895 ± 0.041

4.311 ± 0.03

6.9 ± 0.039

1.742 ± 0.014

7.512 ± 0.043

6.787 ± 0.036

8.855 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.152 ± 0.02

4.406 ± 0.028

3.133 ± 0.023

3.187 ± 0.023

4.814 ± 0.03

5.878 ± 0.029

5.124 ± 0.033

6.739 ± 0.034

0.884 ± 0.013

4.287 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski