Rhizophagus sp. RF1 medium virus
Average proteome isoelectric point is 7.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2RWR2|E2RWR2_9VIRU Putative coat protein OS=Rhizophagus sp. RF1 medium virus OX=758865 GN=CP PE=4 SV=1
MM1 pKa = 8.01 RR2 pKa = 11.84 LPPFKK7 pKa = 9.8 PDD9 pKa = 2.95 GVIYY13 pKa = 10.3 EE14 pKa = 4.55 SYY16 pKa = 11.04 CSLFNLNDD24 pKa = 3.24 IKK26 pKa = 11.38 DD27 pKa = 4.01 VFNAPANFQIMKK39 pKa = 9.72 YY40 pKa = 10.02 EE41 pKa = 4.58 PIFVNSRR48 pKa = 11.84 PRR50 pKa = 11.84 YY51 pKa = 9.26 LYY53 pKa = 10.01 PHH55 pKa = 7.24 DD56 pKa = 3.63 WDD58 pKa = 3.98 YY59 pKa = 11.94 TDD61 pKa = 4.46 GAGAGQSIEE70 pKa = 4.26 VNGVRR75 pKa = 11.84 CFFSDD80 pKa = 3.88 IYY82 pKa = 9.8 SCRR85 pKa = 11.84 WNRR88 pKa = 11.84 FNLNLYY94 pKa = 9.56 SDD96 pKa = 4.16 SVKK99 pKa = 10.64 LVNPKK104 pKa = 10.11 SQTVDD109 pKa = 3.1 MTTLTPEE116 pKa = 4.29 DD117 pKa = 4.84 LMDD120 pKa = 4.47 IDD122 pKa = 4.18 QDD124 pKa = 3.38 NTRR127 pKa = 11.84 IYY129 pKa = 11.14 VFGRR133 pKa = 11.84 LDD135 pKa = 3.24 LSHH138 pKa = 7.75 PYY140 pKa = 9.98 FSLDD144 pKa = 3.52 FPSLEE149 pKa = 4.11 SAATKK154 pKa = 9.98 IRR156 pKa = 11.84 PSLYY160 pKa = 8.85 FARR163 pKa = 11.84 DD164 pKa = 3.16 GRR166 pKa = 11.84 RR167 pKa = 11.84 LVVPILASAIGKK179 pKa = 9.88 NYY181 pKa = 8.64 LTYY184 pKa = 10.62 QNIRR188 pKa = 11.84 NVIYY192 pKa = 10.6 GCLDD196 pKa = 3.37 DD197 pKa = 5.96 RR198 pKa = 11.84 DD199 pKa = 4.35 NLPEE203 pKa = 3.92 VYY205 pKa = 9.8 KK206 pKa = 11.06 FNVSWVCQQPFLPQDD221 pKa = 3.11 KK222 pKa = 10.94 DD223 pKa = 3.67 FFSFNQEE230 pKa = 3.26 VGRR233 pKa = 11.84 WCFVATVNEE242 pKa = 4.05 NTPLHH247 pKa = 6.12 IKK249 pKa = 10.57 YY250 pKa = 8.17 LTKK253 pKa = 10.86 NLLDD257 pKa = 3.58 QMRR260 pKa = 11.84 KK261 pKa = 8.96 QMTNDD266 pKa = 2.65 SSLRR270 pKa = 11.84 HH271 pKa = 4.85 YY272 pKa = 10.94 QSTEE276 pKa = 3.57 LSHH279 pKa = 7.02 LNHH282 pKa = 6.2 EE283 pKa = 4.74 QFANWVVKK291 pKa = 10.15 VKK293 pKa = 10.62 NIKK296 pKa = 9.76 GLHH299 pKa = 5.61 EE300 pKa = 4.33 KK301 pKa = 8.81 TWDD304 pKa = 3.33 KK305 pKa = 11.36 YY306 pKa = 11.18 RR307 pKa = 11.84 LGSDD311 pKa = 3.57 SVIDD315 pKa = 3.61 QYY317 pKa = 11.82 VINYY321 pKa = 7.39 DD322 pKa = 3.75 TAIKK326 pKa = 9.99 EE327 pKa = 4.12 YY328 pKa = 10.52 QKK330 pKa = 11.63 LLGSYY335 pKa = 8.89 VNKK338 pKa = 10.1 EE339 pKa = 4.11 RR340 pKa = 11.84 IDD342 pKa = 3.47 IDD344 pKa = 4.11 SNLVDD349 pKa = 4.07 EE350 pKa = 5.02 LSGLGSVNEE359 pKa = 3.89 KK360 pKa = 10.67 VKK362 pKa = 10.26 WDD364 pKa = 3.46 RR365 pKa = 11.84 CGDD368 pKa = 3.46 NRR370 pKa = 11.84 DD371 pKa = 3.64 RR372 pKa = 11.84 LPFKK376 pKa = 10.9 DD377 pKa = 4.95 DD378 pKa = 4.18 FYY380 pKa = 11.88 NPDD383 pKa = 3.22 EE384 pKa = 4.46 SGRR387 pKa = 11.84 RR388 pKa = 11.84 YY389 pKa = 10.41 SHH391 pKa = 6.6 VFMNPSFKK399 pKa = 10.56 IFGVRR404 pKa = 11.84 LQRR407 pKa = 11.84 LLSMLDD413 pKa = 3.3 CVIDD417 pKa = 4.06 VKK419 pKa = 10.77 HH420 pKa = 6.88 DD421 pKa = 3.62 KK422 pKa = 10.72 YY423 pKa = 11.18 LQDD426 pKa = 3.57 VFSMVYY432 pKa = 9.9 CVAEE436 pKa = 3.79 VSRR439 pKa = 11.84 RR440 pKa = 11.84 FKK442 pKa = 11.25 LLGDD446 pKa = 3.71 FMKK449 pKa = 10.34 KK450 pKa = 10.28 YY451 pKa = 10.72 GSNLTKK457 pKa = 10.46 DD458 pKa = 2.79 WYY460 pKa = 11.13 VYY462 pKa = 11.25 VNLEE466 pKa = 3.75 TAIGYY471 pKa = 7.59 KK472 pKa = 9.1 TALPTDD478 pKa = 4.09 EE479 pKa = 4.31 FVHH482 pKa = 6.46 MGGEE486 pKa = 4.2 WIVGDD491 pKa = 4.23 IEE493 pKa = 5.51 HH494 pKa = 7.29 IAPVPNGQLKK504 pKa = 9.45 FYY506 pKa = 9.04 EE507 pKa = 4.13 WLVEE511 pKa = 4.55 GIEE514 pKa = 4.09 STLRR518 pKa = 11.84 LKK520 pKa = 10.86 GKK522 pKa = 10.14 SKK524 pKa = 10.41 IMYY527 pKa = 7.1 EE528 pKa = 4.03 KK529 pKa = 10.23 PIEE532 pKa = 4.17 FDD534 pKa = 3.03 EE535 pKa = 5.18 HH536 pKa = 6.13 GNPMEE541 pKa = 4.3 TLDD544 pKa = 3.54 QFSYY548 pKa = 11.12 DD549 pKa = 3.18 VSKK552 pKa = 10.72 QLSYY556 pKa = 11.0 RR557 pKa = 11.84 DD558 pKa = 3.67 YY559 pKa = 11.45 VYY561 pKa = 10.61 GCTWSRR567 pKa = 11.84 RR568 pKa = 11.84 GSSSIKK574 pKa = 8.28 TKK576 pKa = 10.65 LRR578 pKa = 11.84 AEE580 pKa = 4.11 INGKK584 pKa = 8.31 KK585 pKa = 10.22 VKK587 pKa = 10.61 LSEE590 pKa = 4.38 TKK592 pKa = 9.17 NTLAIYY598 pKa = 10.72 GDD600 pKa = 3.85 LEE602 pKa = 5.03 KK603 pKa = 10.5 ILKK606 pKa = 10.42 DD607 pKa = 3.34 SLDD610 pKa = 3.54 PDD612 pKa = 3.71 RR613 pKa = 11.84 PQWGKK618 pKa = 10.81 AIRR621 pKa = 11.84 KK622 pKa = 9.16 RR623 pKa = 11.84 EE624 pKa = 3.87 RR625 pKa = 11.84 GKK627 pKa = 10.1 IRR629 pKa = 11.84 AVLGMDD635 pKa = 3.49 VEE637 pKa = 5.31 TYY639 pKa = 11.24 LPMSYY644 pKa = 9.45 IDD646 pKa = 6.28 DD647 pKa = 4.91 IIQPYY652 pKa = 10.11 IIGNEE657 pKa = 3.96 LSNLWEE663 pKa = 4.41 TNFQRR668 pKa = 11.84 HH669 pKa = 5.56 AKK671 pKa = 10.3 DD672 pKa = 2.96 VDD674 pKa = 3.69 RR675 pKa = 11.84 LILSIEE681 pKa = 3.92 KK682 pKa = 9.31 TKK684 pKa = 10.52 WFIPLDD690 pKa = 3.45 QSGFDD695 pKa = 3.45 HH696 pKa = 7.15 QINNNMLLLTLVIIKK711 pKa = 9.84 SWLLSRR717 pKa = 11.84 VKK719 pKa = 10.96 YY720 pKa = 10.51 KK721 pKa = 11.11 GDD723 pKa = 3.33 VEE725 pKa = 4.25 RR726 pKa = 11.84 CIEE729 pKa = 4.2 IIIKK733 pKa = 10.32 RR734 pKa = 11.84 LALTNSEE741 pKa = 4.41 VNVDD745 pKa = 3.85 DD746 pKa = 5.43 KK747 pKa = 11.75 INIPVEE753 pKa = 4.0 RR754 pKa = 11.84 GILSGWKK761 pKa = 7.04 WTAFMDD767 pKa = 3.57 TLINIGEE774 pKa = 4.36 VNVALRR780 pKa = 11.84 TCEE783 pKa = 3.91 YY784 pKa = 10.62 YY785 pKa = 11.23 GLVCNYY791 pKa = 10.41 KK792 pKa = 10.36 LDD794 pKa = 4.0 AQGDD798 pKa = 3.95 DD799 pKa = 4.38 DD800 pKa = 6.96 DD801 pKa = 6.99 IIIDD805 pKa = 4.15 DD806 pKa = 4.76 LLTGLVMCEE815 pKa = 3.49 AYY817 pKa = 10.08 RR818 pKa = 11.84 YY819 pKa = 9.91 CNLKK823 pKa = 10.23 INPAKK828 pKa = 10.46 FFIKK832 pKa = 10.74 NNIDD836 pKa = 3.08 EE837 pKa = 4.48 YY838 pKa = 11.03 LRR840 pKa = 11.84 RR841 pKa = 11.84 VYY843 pKa = 11.11 VDD845 pKa = 4.0 GVSLGYY851 pKa = 8.48 PARR854 pKa = 11.84 MFLTFFEE861 pKa = 5.1 SNPIRR866 pKa = 11.84 NVPLSEE872 pKa = 3.9 MSEE875 pKa = 3.9 LRR877 pKa = 11.84 SVIKK881 pKa = 10.7 NWMDD885 pKa = 4.77 LIRR888 pKa = 11.84 RR889 pKa = 11.84 RR890 pKa = 11.84 SLNNEE895 pKa = 3.47 RR896 pKa = 11.84 WVKK899 pKa = 10.02 LVKK902 pKa = 10.67 LMEE905 pKa = 4.75 RR906 pKa = 11.84 DD907 pKa = 2.89 ILIRR911 pKa = 11.84 VNKK914 pKa = 8.77 NRR916 pKa = 11.84 KK917 pKa = 8.77 ISSLSLEE924 pKa = 4.57 TILL927 pKa = 5.58
Molecular weight: 108.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.42
IPC2_protein 6.59
IPC_protein 6.62
Toseland 6.59
ProMoST 7.088
Dawson 7.132
Bjellqvist 7.176
Wikipedia 7.088
Rodwell 7.132
Grimsley 6.678
Solomon 7.146
Lehninger 7.146
Nozaki 7.439
DTASelect 7.41
Thurlkill 7.454
EMBOSS 7.454
Sillero 7.556
Patrickios 4.177
IPC_peptide 7.146
IPC2_peptide 6.912
IPC2.peptide.svr19 6.875
Protein with the highest isoelectric point:
>tr|E2RWR2|E2RWR2_9VIRU Putative coat protein OS=Rhizophagus sp. RF1 medium virus OX=758865 GN=CP PE=4 SV=1
MM1 pKa = 7.71 SLRR4 pKa = 11.84 LRR6 pKa = 11.84 INTKK10 pKa = 9.77 GLAVVIPSGMGKK22 pKa = 7.1 TTLSNKK28 pKa = 9.55 FPSLFLDD35 pKa = 3.73 IDD37 pKa = 4.63 DD38 pKa = 5.05 IVWKK42 pKa = 9.71 KK43 pKa = 10.32 HH44 pKa = 5.94 KK45 pKa = 10.24 ILIGIINKK53 pKa = 9.32 AFAWKK58 pKa = 10.18 FGSDD62 pKa = 3.14 LTKK65 pKa = 10.92 NILRR69 pKa = 11.84 RR70 pKa = 11.84 KK71 pKa = 8.02 WLWNDD76 pKa = 3.22 TLSRR80 pKa = 11.84 KK81 pKa = 9.35 VLLCHH86 pKa = 7.01 SPDD89 pKa = 3.54 QLPSGVRR96 pKa = 11.84 VLCILNSNSLNVNKK110 pKa = 10.09 IKK112 pKa = 10.74 KK113 pKa = 8.27 EE114 pKa = 3.8 RR115 pKa = 11.84 KK116 pKa = 8.42 ADD118 pKa = 3.5 NVRR121 pKa = 11.84 ISLQNRR127 pKa = 11.84 HH128 pKa = 5.88 SLWEE132 pKa = 3.89 NYY134 pKa = 9.46 RR135 pKa = 11.84 DD136 pKa = 3.49 EE137 pKa = 4.52 EE138 pKa = 4.72 NYY140 pKa = 10.53 FEE142 pKa = 4.26 VQSNKK147 pKa = 9.65 VMLDD151 pKa = 3.33 TIINCLIKK159 pKa = 10.92 YY160 pKa = 10.39 VDD162 pKa = 4.21 FEE164 pKa = 4.57 KK165 pKa = 11.12 PNDD168 pKa = 3.61 NTKK171 pKa = 10.9 LLVPSKK177 pKa = 10.79 DD178 pKa = 3.73 YY179 pKa = 10.59 IGKK182 pKa = 9.1 FVRR185 pKa = 11.84 KK186 pKa = 8.95 EE187 pKa = 3.18 RR188 pKa = 11.84 YY189 pKa = 8.8 LRR191 pKa = 11.84 YY192 pKa = 9.19 KK193 pKa = 9.98 VKK195 pKa = 10.81 YY196 pKa = 9.55 DD197 pKa = 3.04 ISEE200 pKa = 4.77 PIVMSVRR207 pKa = 11.84 EE208 pKa = 3.84 YY209 pKa = 11.43 MMIKK213 pKa = 10.25 DD214 pKa = 4.01 YY215 pKa = 10.89 WRR217 pKa = 11.84 DD218 pKa = 3.38 LYY220 pKa = 10.16 VHH222 pKa = 6.92 RR223 pKa = 11.84 KK224 pKa = 4.96 WFKK227 pKa = 9.26 IEE229 pKa = 4.54 SYY231 pKa = 11.25 GKK233 pKa = 9.73 DD234 pKa = 3.29 EE235 pKa = 4.2 KK236 pKa = 11.14 HH237 pKa = 6.58 SLTDD241 pKa = 3.35 EE242 pKa = 4.35 SVNDD246 pKa = 3.72 QIVTKK251 pKa = 10.29 ILPLIDD257 pKa = 4.38 KK258 pKa = 7.63 EE259 pKa = 4.62 TLFVLKK265 pKa = 10.1 EE266 pKa = 3.97 YY267 pKa = 10.39 RR268 pKa = 11.84 WDD270 pKa = 3.69 RR271 pKa = 11.84 MITNLMGIKK280 pKa = 10.24 NSEE283 pKa = 4.17 LVTNWMYY290 pKa = 10.87 TPAYY294 pKa = 9.18 LGGVGIQLDD303 pKa = 4.3 GLRR306 pKa = 11.84 KK307 pKa = 8.16 PMSYY311 pKa = 8.39 ITSSEE316 pKa = 4.27 SIITPFVKK324 pKa = 10.08 YY325 pKa = 9.55 PKK327 pKa = 9.83 SEE329 pKa = 3.97 RR330 pKa = 11.84 VEE332 pKa = 4.26 NLTDD336 pKa = 3.98 MILKK340 pKa = 9.97 RR341 pKa = 11.84 SKK343 pKa = 10.69 FSVVDD348 pKa = 3.96 NIQVYY353 pKa = 10.01 EE354 pKa = 4.54 GSQSTNKK361 pKa = 9.44 IFKK364 pKa = 10.27 SVGILRR370 pKa = 11.84 FHH372 pKa = 7.07 KK373 pKa = 10.95 VIDD376 pKa = 4.27 NINFRR381 pKa = 11.84 KK382 pKa = 9.14 FEE384 pKa = 4.01 WMISGVDD391 pKa = 3.95 FCKK394 pKa = 10.95 SMIRR398 pKa = 11.84 GVEE401 pKa = 4.0 LPIKK405 pKa = 9.61 MIRR408 pKa = 11.84 PIFNSNIRR416 pKa = 11.84 INQHH420 pKa = 5.77 AQTIWINGLMRR431 pKa = 11.84 AKK433 pKa = 10.61 DD434 pKa = 3.6 EE435 pKa = 4.87 EE436 pKa = 4.32 ITNEE440 pKa = 3.72 ILSEE444 pKa = 4.07 LFVNVSEE451 pKa = 5.62 IIDD454 pKa = 3.46 KK455 pKa = 10.61 RR456 pKa = 11.84 RR457 pKa = 11.84 NFSKK461 pKa = 10.55 IFWRR465 pKa = 11.84 DD466 pKa = 3.23 WLCGRR471 pKa = 11.84 LEE473 pKa = 4.05 YY474 pKa = 10.45 QKK476 pKa = 10.33 PINMWINDD484 pKa = 4.4 NIVSTYY490 pKa = 10.33 FIINLNRR497 pKa = 11.84 LLSLNCNVDD506 pKa = 3.33 YY507 pKa = 11.52 GMLKK511 pKa = 10.51 SMCKK515 pKa = 9.71 IIEE518 pKa = 4.13 KK519 pKa = 9.89 TEE521 pKa = 3.87 LNNIIRR527 pKa = 11.84 YY528 pKa = 8.74 LRR530 pKa = 11.84 EE531 pKa = 3.63 RR532 pKa = 11.84 KK533 pKa = 7.96 ITLMM537 pKa = 4.36
Molecular weight: 63.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.967
IPC2_protein 8.96
IPC_protein 8.843
Toseland 9.677
ProMoST 9.37
Dawson 9.911
Bjellqvist 9.589
Wikipedia 10.058
Rodwell 10.335
Grimsley 9.97
Solomon 9.926
Lehninger 9.897
Nozaki 9.736
DTASelect 9.56
Thurlkill 9.765
EMBOSS 10.101
Sillero 9.838
Patrickios 5.982
IPC_peptide 9.926
IPC2_peptide 8.112
IPC2.peptide.svr19 8.08
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1464
537
927
732.0
85.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.391 ± 0.589
1.503 ± 0.108
7.104 ± 0.823
5.464 ± 0.066
4.167 ± 0.139
4.508 ± 0.425
1.434 ± 0.071
8.675 ± 1.254
8.06 ± 0.88
9.973 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.801 ± 0.4
6.967 ± 0.463
3.415 ± 0.337
2.459 ± 0.324
6.352 ± 0.09
6.899 ± 0.197
3.962 ± 0.073
6.831 ± 0.17
2.254 ± 0.191
4.781 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here