Amino acid dipepetide frequency for Mycobacterium phage Froghopper

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.788AlaAla: 13.788 ± 1.419
0.788AlaCys: 0.788 ± 0.239
7.025AlaAsp: 7.025 ± 0.851
6.763AlaGlu: 6.763 ± 0.769
3.086AlaPhe: 3.086 ± 0.51
8.01AlaGly: 8.01 ± 0.806
1.97AlaHis: 1.97 ± 0.518
4.793AlaIle: 4.793 ± 0.612
3.742AlaLys: 3.742 ± 0.551
8.798AlaLeu: 8.798 ± 0.804
2.101AlaMet: 2.101 ± 0.383
2.823AlaAsn: 2.823 ± 0.419
5.581AlaPro: 5.581 ± 0.89
3.217AlaGln: 3.217 ± 0.473
6.369AlaArg: 6.369 ± 0.68
5.121AlaSer: 5.121 ± 0.807
6.303AlaThr: 6.303 ± 0.65
8.864AlaVal: 8.864 ± 0.751
1.838AlaTrp: 1.838 ± 0.37
2.758AlaTyr: 2.758 ± 0.417
0.0AlaXaa: 0.0 ± 0.0
Cys
0.657CysAla: 0.657 ± 0.241
0.0CysCys: 0.0 ± 0.0
0.328CysAsp: 0.328 ± 0.137
0.591CysGlu: 0.591 ± 0.198
0.197CysPhe: 0.197 ± 0.119
0.657CysGly: 0.657 ± 0.253
0.197CysHis: 0.197 ± 0.117
0.197CysIle: 0.197 ± 0.113
0.263CysLys: 0.263 ± 0.138
0.263CysLeu: 0.263 ± 0.118
0.0CysMet: 0.0 ± 0.0
0.197CysAsn: 0.197 ± 0.115
0.394CysPro: 0.394 ± 0.199
0.197CysGln: 0.197 ± 0.108
0.525CysArg: 0.525 ± 0.174
0.131CysSer: 0.131 ± 0.103
0.394CysThr: 0.394 ± 0.18
0.525CysVal: 0.525 ± 0.178
0.131CysTrp: 0.131 ± 0.093
0.197CysTyr: 0.197 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
6.631AspAla: 6.631 ± 0.769
0.591AspCys: 0.591 ± 0.204
4.727AspAsp: 4.727 ± 0.559
3.939AspGlu: 3.939 ± 0.492
2.561AspPhe: 2.561 ± 0.38
6.369AspGly: 6.369 ± 0.661
1.379AspHis: 1.379 ± 0.315
2.823AspIle: 2.823 ± 0.454
2.495AspLys: 2.495 ± 0.419
7.156AspLeu: 7.156 ± 0.695
1.313AspMet: 1.313 ± 0.257
1.379AspAsn: 1.379 ± 0.312
4.662AspPro: 4.662 ± 0.578
1.51AspGln: 1.51 ± 0.311
3.742AspArg: 3.742 ± 0.432
3.48AspSer: 3.48 ± 0.502
3.742AspThr: 3.742 ± 0.491
4.859AspVal: 4.859 ± 0.536
1.707AspTrp: 1.707 ± 0.385
2.035AspTyr: 2.035 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
6.172GluAla: 6.172 ± 0.8
0.263GluCys: 0.263 ± 0.15
4.399GluAsp: 4.399 ± 0.534
4.793GluGlu: 4.793 ± 0.598
1.838GluPhe: 1.838 ± 0.386
3.808GluGly: 3.808 ± 0.449
1.05GluHis: 1.05 ± 0.296
3.217GluIle: 3.217 ± 0.393
2.758GluLys: 2.758 ± 0.464
7.091GluLeu: 7.091 ± 0.62
1.379GluMet: 1.379 ± 0.256
1.576GluAsn: 1.576 ± 0.305
2.758GluPro: 2.758 ± 0.496
2.889GluGln: 2.889 ± 0.426
3.677GluArg: 3.677 ± 0.622
3.808GluSer: 3.808 ± 0.505
3.939GluThr: 3.939 ± 0.475
5.975GluVal: 5.975 ± 0.664
1.444GluTrp: 1.444 ± 0.402
1.97GluTyr: 1.97 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
2.626PheAla: 2.626 ± 0.299
0.197PheCys: 0.197 ± 0.143
2.758PheAsp: 2.758 ± 0.345
1.773PheGlu: 1.773 ± 0.331
0.46PhePhe: 0.46 ± 0.165
3.348PheGly: 3.348 ± 0.474
0.657PheHis: 0.657 ± 0.25
1.313PheIle: 1.313 ± 0.27
1.247PheLys: 1.247 ± 0.328
2.495PheLeu: 2.495 ± 0.484
0.722PheMet: 0.722 ± 0.211
0.919PheAsn: 0.919 ± 0.251
1.641PhePro: 1.641 ± 0.359
1.116PheGln: 1.116 ± 0.263
2.101PheArg: 2.101 ± 0.387
2.561PheSer: 2.561 ± 0.506
1.773PheThr: 1.773 ± 0.34
1.97PheVal: 1.97 ± 0.342
0.591PheTrp: 0.591 ± 0.184
0.854PheTyr: 0.854 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
7.288GlyAla: 7.288 ± 0.858
0.722GlyCys: 0.722 ± 0.285
6.106GlyAsp: 6.106 ± 0.49
4.727GlyGlu: 4.727 ± 0.618
2.955GlyPhe: 2.955 ± 0.479
6.959GlyGly: 6.959 ± 1.105
1.576GlyHis: 1.576 ± 0.359
4.662GlyIle: 4.662 ± 0.718
3.086GlyLys: 3.086 ± 0.487
7.419GlyLeu: 7.419 ± 1.02
1.904GlyMet: 1.904 ± 0.373
3.151GlyAsn: 3.151 ± 0.42
4.333GlyPro: 4.333 ± 0.556
2.495GlyGln: 2.495 ± 0.344
4.268GlyArg: 4.268 ± 0.554
5.843GlySer: 5.843 ± 0.612
4.99GlyThr: 4.99 ± 0.713
5.581GlyVal: 5.581 ± 0.679
2.035GlyTrp: 2.035 ± 0.341
2.692GlyTyr: 2.692 ± 0.362
0.0GlyXaa: 0.0 ± 0.0
His
1.838HisAla: 1.838 ± 0.376
0.131HisCys: 0.131 ± 0.097
1.313HisAsp: 1.313 ± 0.235
1.576HisGlu: 1.576 ± 0.306
0.854HisPhe: 0.854 ± 0.243
1.116HisGly: 1.116 ± 0.264
0.788HisHis: 0.788 ± 0.253
0.985HisIle: 0.985 ± 0.223
0.854HisLys: 0.854 ± 0.318
1.576HisLeu: 1.576 ± 0.403
0.131HisMet: 0.131 ± 0.097
0.394HisAsn: 0.394 ± 0.185
1.379HisPro: 1.379 ± 0.329
0.985HisGln: 0.985 ± 0.197
1.51HisArg: 1.51 ± 0.323
0.722HisSer: 0.722 ± 0.21
1.247HisThr: 1.247 ± 0.358
1.641HisVal: 1.641 ± 0.37
0.591HisTrp: 0.591 ± 0.193
0.722HisTyr: 0.722 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
6.172IleAla: 6.172 ± 0.752
0.328IleCys: 0.328 ± 0.136
3.545IleAsp: 3.545 ± 0.393
3.348IleGlu: 3.348 ± 0.471
0.854IlePhe: 0.854 ± 0.247
4.136IleGly: 4.136 ± 0.516
1.116IleHis: 1.116 ± 0.244
1.838IleIle: 1.838 ± 0.316
1.838IleLys: 1.838 ± 0.346
3.677IleLeu: 3.677 ± 0.487
0.788IleMet: 0.788 ± 0.238
1.773IleAsn: 1.773 ± 0.35
3.151IlePro: 3.151 ± 0.43
1.641IleGln: 1.641 ± 0.316
3.742IleArg: 3.742 ± 0.506
3.348IleSer: 3.348 ± 0.507
3.086IleThr: 3.086 ± 0.482
2.364IleVal: 2.364 ± 0.56
0.657IleTrp: 0.657 ± 0.195
1.576IleTyr: 1.576 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
3.545LysAla: 3.545 ± 0.68
0.197LysCys: 0.197 ± 0.107
2.232LysAsp: 2.232 ± 0.374
1.838LysGlu: 1.838 ± 0.384
1.576LysPhe: 1.576 ± 0.318
2.692LysGly: 2.692 ± 0.402
1.247LysHis: 1.247 ± 0.338
2.626LysIle: 2.626 ± 0.556
1.707LysLys: 1.707 ± 0.402
3.283LysLeu: 3.283 ± 0.479
0.722LysMet: 0.722 ± 0.198
1.379LysAsn: 1.379 ± 0.239
2.955LysPro: 2.955 ± 0.441
1.838LysGln: 1.838 ± 0.407
2.823LysArg: 2.823 ± 0.425
2.364LysSer: 2.364 ± 0.385
1.97LysThr: 1.97 ± 0.365
2.955LysVal: 2.955 ± 0.49
0.657LysTrp: 0.657 ± 0.193
0.919LysTyr: 0.919 ± 0.271
0.0LysXaa: 0.0 ± 0.0
Leu
10.045LeuAla: 10.045 ± 0.9
0.263LeuCys: 0.263 ± 0.114
6.172LeuAsp: 6.172 ± 0.532
5.909LeuGlu: 5.909 ± 0.71
1.904LeuPhe: 1.904 ± 0.402
7.616LeuGly: 7.616 ± 0.928
1.51LeuHis: 1.51 ± 0.332
4.727LeuIle: 4.727 ± 0.594
3.808LeuLys: 3.808 ± 0.503
5.909LeuLeu: 5.909 ± 0.563
1.444LeuMet: 1.444 ± 0.263
2.955LeuAsn: 2.955 ± 0.402
6.172LeuPro: 6.172 ± 0.688
2.626LeuGln: 2.626 ± 0.418
5.909LeuArg: 5.909 ± 0.513
5.515LeuSer: 5.515 ± 0.543
6.237LeuThr: 6.237 ± 0.464
4.596LeuVal: 4.596 ± 0.707
1.182LeuTrp: 1.182 ± 0.34
2.495LeuTyr: 2.495 ± 0.417
0.0LeuXaa: 0.0 ± 0.0
Met
2.429MetAla: 2.429 ± 0.323
0.066MetCys: 0.066 ± 0.064
1.379MetAsp: 1.379 ± 0.324
1.444MetGlu: 1.444 ± 0.324
0.525MetPhe: 0.525 ± 0.167
1.247MetGly: 1.247 ± 0.335
0.197MetHis: 0.197 ± 0.107
0.591MetIle: 0.591 ± 0.212
0.854MetLys: 0.854 ± 0.23
1.182MetLeu: 1.182 ± 0.277
0.131MetMet: 0.131 ± 0.091
0.854MetAsn: 0.854 ± 0.179
1.116MetPro: 1.116 ± 0.256
0.46MetGln: 0.46 ± 0.175
1.576MetArg: 1.576 ± 0.382
2.101MetSer: 2.101 ± 0.419
2.232MetThr: 2.232 ± 0.35
1.05MetVal: 1.05 ± 0.26
0.263MetTrp: 0.263 ± 0.121
0.46MetTyr: 0.46 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
2.626AsnAla: 2.626 ± 0.574
0.263AsnCys: 0.263 ± 0.244
2.101AsnAsp: 2.101 ± 0.396
1.838AsnGlu: 1.838 ± 0.336
0.919AsnPhe: 0.919 ± 0.314
3.414AsnGly: 3.414 ± 0.495
0.788AsnHis: 0.788 ± 0.23
1.379AsnIle: 1.379 ± 0.367
0.46AsnLys: 0.46 ± 0.167
2.232AsnLeu: 2.232 ± 0.316
0.657AsnMet: 0.657 ± 0.192
0.788AsnAsn: 0.788 ± 0.239
2.429AsnPro: 2.429 ± 0.408
0.854AsnGln: 0.854 ± 0.213
1.444AsnArg: 1.444 ± 0.318
2.167AsnSer: 2.167 ± 0.45
1.97AsnThr: 1.97 ± 0.271
2.495AsnVal: 2.495 ± 0.474
0.722AsnTrp: 0.722 ± 0.21
1.379AsnTyr: 1.379 ± 0.299
0.0AsnXaa: 0.0 ± 0.0
Pro
6.172ProAla: 6.172 ± 0.821
0.394ProCys: 0.394 ± 0.166
4.071ProAsp: 4.071 ± 0.455
4.596ProGlu: 4.596 ± 0.633
2.167ProPhe: 2.167 ± 0.464
5.055ProGly: 5.055 ± 0.6
1.05ProHis: 1.05 ± 0.36
2.561ProIle: 2.561 ± 0.404
2.101ProLys: 2.101 ± 0.35
4.924ProLeu: 4.924 ± 0.588
0.854ProMet: 0.854 ± 0.235
1.444ProAsn: 1.444 ± 0.299
3.086ProPro: 3.086 ± 0.512
1.379ProGln: 1.379 ± 0.292
2.955ProArg: 2.955 ± 0.507
3.742ProSer: 3.742 ± 0.556
4.268ProThr: 4.268 ± 0.594
4.268ProVal: 4.268 ± 0.484
0.919ProTrp: 0.919 ± 0.283
1.51ProTyr: 1.51 ± 0.317
0.0ProXaa: 0.0 ± 0.0
Gln
3.348GlnAla: 3.348 ± 0.515
0.066GlnCys: 0.066 ± 0.067
1.247GlnAsp: 1.247 ± 0.346
1.576GlnGlu: 1.576 ± 0.278
1.116GlnPhe: 1.116 ± 0.254
2.495GlnGly: 2.495 ± 0.356
0.788GlnHis: 0.788 ± 0.212
2.823GlnIle: 2.823 ± 0.652
1.379GlnLys: 1.379 ± 0.241
3.874GlnLeu: 3.874 ± 0.544
0.919GlnMet: 0.919 ± 0.247
0.722GlnAsn: 0.722 ± 0.208
1.838GlnPro: 1.838 ± 0.371
1.838GlnGln: 1.838 ± 0.378
1.707GlnArg: 1.707 ± 0.369
1.773GlnSer: 1.773 ± 0.328
1.51GlnThr: 1.51 ± 0.333
2.495GlnVal: 2.495 ± 0.356
0.591GlnTrp: 0.591 ± 0.167
0.525GlnTyr: 0.525 ± 0.166
0.0GlnXaa: 0.0 ± 0.0
Arg
5.909ArgAla: 5.909 ± 0.655
0.525ArgCys: 0.525 ± 0.159
3.677ArgAsp: 3.677 ± 0.48
4.136ArgGlu: 4.136 ± 0.609
2.035ArgPhe: 2.035 ± 0.393
4.399ArgGly: 4.399 ± 0.645
1.182ArgHis: 1.182 ± 0.292
3.283ArgIle: 3.283 ± 0.548
2.823ArgLys: 2.823 ± 0.477
6.106ArgLeu: 6.106 ± 0.738
1.838ArgMet: 1.838 ± 0.332
2.101ArgAsn: 2.101 ± 0.428
2.626ArgPro: 2.626 ± 0.445
1.641ArgGln: 1.641 ± 0.331
5.055ArgArg: 5.055 ± 0.661
3.677ArgSer: 3.677 ± 0.602
3.086ArgThr: 3.086 ± 0.483
5.187ArgVal: 5.187 ± 0.594
1.182ArgTrp: 1.182 ± 0.343
1.444ArgTyr: 1.444 ± 0.272
0.0ArgXaa: 0.0 ± 0.0
Ser
6.566SerAla: 6.566 ± 0.824
0.46SerCys: 0.46 ± 0.205
3.348SerAsp: 3.348 ± 0.422
4.333SerGlu: 4.333 ± 0.588
2.167SerPhe: 2.167 ± 0.45
6.303SerGly: 6.303 ± 0.718
1.313SerHis: 1.313 ± 0.302
3.086SerIle: 3.086 ± 0.428
2.823SerLys: 2.823 ± 0.467
5.515SerLeu: 5.515 ± 0.72
1.707SerMet: 1.707 ± 0.38
2.298SerAsn: 2.298 ± 0.344
3.348SerPro: 3.348 ± 0.55
2.101SerGln: 2.101 ± 0.295
2.692SerArg: 2.692 ± 0.394
4.136SerSer: 4.136 ± 0.703
3.48SerThr: 3.48 ± 0.526
3.414SerVal: 3.414 ± 0.424
1.182SerTrp: 1.182 ± 0.342
1.116SerTyr: 1.116 ± 0.313
0.0SerXaa: 0.0 ± 0.0
Thr
6.5ThrAla: 6.5 ± 0.734
0.197ThrCys: 0.197 ± 0.098
4.071ThrAsp: 4.071 ± 0.609
4.333ThrGlu: 4.333 ± 0.579
2.232ThrPhe: 2.232 ± 0.416
6.566ThrGly: 6.566 ± 0.703
1.313ThrHis: 1.313 ± 0.378
2.561ThrIle: 2.561 ± 0.579
2.692ThrLys: 2.692 ± 0.328
5.975ThrLeu: 5.975 ± 0.726
1.182ThrMet: 1.182 ± 0.269
1.576ThrAsn: 1.576 ± 0.364
4.005ThrPro: 4.005 ± 0.547
1.838ThrGln: 1.838 ± 0.351
3.414ThrArg: 3.414 ± 0.579
3.545ThrSer: 3.545 ± 0.555
4.53ThrThr: 4.53 ± 0.564
5.581ThrVal: 5.581 ± 0.707
0.985ThrTrp: 0.985 ± 0.279
1.904ThrTyr: 1.904 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
7.222ValAla: 7.222 ± 0.763
0.394ValCys: 0.394 ± 0.19
5.646ValAsp: 5.646 ± 0.537
4.333ValGlu: 4.333 ± 0.461
2.561ValPhe: 2.561 ± 0.358
4.727ValGly: 4.727 ± 0.746
1.379ValHis: 1.379 ± 0.269
3.217ValIle: 3.217 ± 0.452
3.086ValLys: 3.086 ± 0.481
4.99ValLeu: 4.99 ± 0.695
1.313ValMet: 1.313 ± 0.334
2.955ValAsn: 2.955 ± 0.452
3.874ValPro: 3.874 ± 0.45
2.429ValGln: 2.429 ± 0.516
4.99ValArg: 4.99 ± 0.577
4.793ValSer: 4.793 ± 0.532
6.303ValThr: 6.303 ± 0.676
4.399ValVal: 4.399 ± 0.648
1.182ValTrp: 1.182 ± 0.295
2.298ValTyr: 2.298 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
1.904TrpAla: 1.904 ± 0.366
0.131TrpCys: 0.131 ± 0.089
1.641TrpAsp: 1.641 ± 0.367
0.854TrpGlu: 0.854 ± 0.239
0.657TrpPhe: 0.657 ± 0.194
1.444TrpGly: 1.444 ± 0.288
0.263TrpHis: 0.263 ± 0.136
0.854TrpIle: 0.854 ± 0.197
0.263TrpLys: 0.263 ± 0.173
1.773TrpLeu: 1.773 ± 0.322
0.328TrpMet: 0.328 ± 0.177
0.46TrpAsn: 0.46 ± 0.194
0.854TrpPro: 0.854 ± 0.268
0.854TrpGln: 0.854 ± 0.243
1.116TrpArg: 1.116 ± 0.35
1.05TrpSer: 1.05 ± 0.282
1.641TrpThr: 1.641 ± 0.355
2.101TrpVal: 2.101 ± 0.307
0.722TrpTrp: 0.722 ± 0.303
0.328TrpTyr: 0.328 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.298TyrAla: 2.298 ± 0.436
0.066TyrCys: 0.066 ± 0.063
1.313TyrAsp: 1.313 ± 0.311
2.101TyrGlu: 2.101 ± 0.345
0.46TyrPhe: 0.46 ± 0.147
2.429TyrGly: 2.429 ± 0.398
0.591TyrHis: 0.591 ± 0.213
1.444TyrIle: 1.444 ± 0.285
1.247TyrLys: 1.247 ± 0.283
2.758TyrLeu: 2.758 ± 0.422
0.657TyrMet: 0.657 ± 0.166
1.182TyrAsn: 1.182 ± 0.336
1.313TyrPro: 1.313 ± 0.299
0.722TyrGln: 0.722 ± 0.191
2.364TyrArg: 2.364 ± 0.384
1.51TyrSer: 1.51 ± 0.329
2.298TyrThr: 2.298 ± 0.423
1.773TyrVal: 1.773 ± 0.424
0.657TyrTrp: 0.657 ± 0.206
0.525TyrTyr: 0.525 ± 0.17
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15232 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski