Termite associated circular virus 4
Average proteome isoelectric point is 7.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1E315|A0A2P1E315_9VIRU Replication associated protein OS=Termite associated circular virus 4 OX=2108552 PE=3 SV=1
MM1 pKa = 8.14 PEE3 pKa = 3.93 FRR5 pKa = 11.84 WQGKK9 pKa = 7.58 YY10 pKa = 10.71 LFLTYY15 pKa = 9.76 PQSDD19 pKa = 4.02 FNLDD23 pKa = 3.63 GAVHH27 pKa = 7.3 RR28 pKa = 11.84 LRR30 pKa = 11.84 EE31 pKa = 3.83 QGGRR35 pKa = 11.84 HH36 pKa = 3.73 VTFVRR41 pKa = 11.84 VCSEE45 pKa = 3.61 QHH47 pKa = 5.52 QSGEE51 pKa = 4.16 LHH53 pKa = 6.13 RR54 pKa = 11.84 HH55 pKa = 5.41 ALLCFGKK62 pKa = 10.23 RR63 pKa = 11.84 VSFTDD68 pKa = 3.07 QRR70 pKa = 11.84 RR71 pKa = 11.84 FDD73 pKa = 3.28 IDD75 pKa = 2.99 GRR77 pKa = 11.84 HH78 pKa = 6.06 PKK80 pKa = 8.67 WEE82 pKa = 4.16 RR83 pKa = 11.84 YY84 pKa = 6.57 TRR86 pKa = 11.84 SPAKK90 pKa = 9.72 AAEE93 pKa = 4.28 YY94 pKa = 9.38 IAKK97 pKa = 10.08 EE98 pKa = 4.1 GEE100 pKa = 4.17 YY101 pKa = 10.33 VDD103 pKa = 3.76 WGEE106 pKa = 4.31 TPDD109 pKa = 4.94 FSTPEE114 pKa = 3.91 CRR116 pKa = 11.84 EE117 pKa = 3.84 SRR119 pKa = 11.84 NEE121 pKa = 3.46 LWGRR125 pKa = 11.84 LLDD128 pKa = 3.93 EE129 pKa = 4.53 ATTPEE134 pKa = 4.2 SFMSLVRR141 pKa = 11.84 QFAPYY146 pKa = 10.76 DD147 pKa = 3.2 FATRR151 pKa = 11.84 YY152 pKa = 8.74 QALHH156 pKa = 5.69 TMATSVFHH164 pKa = 6.81 RR165 pKa = 11.84 RR166 pKa = 11.84 SPHH169 pKa = 5.15 QSPHH173 pKa = 7.56 DD174 pKa = 3.77 MVEE177 pKa = 4.2 DD178 pKa = 4.19 FQLPDD183 pKa = 3.61 TIEE186 pKa = 3.72 RR187 pKa = 11.84 WLEE190 pKa = 3.79 EE191 pKa = 4.14 EE192 pKa = 4.16 FDD194 pKa = 3.66 QEE196 pKa = 4.18 NRR198 pKa = 11.84 PIRR201 pKa = 11.84 PKK203 pKa = 10.75 SLLIVGDD210 pKa = 3.75 SRR212 pKa = 11.84 SGKK215 pKa = 8.7 TSFARR220 pKa = 11.84 CLGHH224 pKa = 7.21 HH225 pKa = 7.15 IYY227 pKa = 10.44 FQGLFNLDD235 pKa = 3.58 KK236 pKa = 10.7 FDD238 pKa = 4.16 PQADD242 pKa = 3.72 YY243 pKa = 11.54 AVFDD247 pKa = 3.85 DD248 pKa = 6.37 CKK250 pKa = 10.72 IKK252 pKa = 10.14 WISNHH257 pKa = 6.18 KK258 pKa = 9.94 SWLGCGGEE266 pKa = 4.7 FEE268 pKa = 4.99 ATDD271 pKa = 3.3 KK272 pKa = 11.32 YY273 pKa = 9.54 RR274 pKa = 11.84 TKK276 pKa = 10.36 RR277 pKa = 11.84 TLSWSGKK284 pKa = 9.53 PSIICCNRR292 pKa = 11.84 GSTWDD297 pKa = 3.2 WRR299 pKa = 11.84 YY300 pKa = 10.53 SEE302 pKa = 4.54 EE303 pKa = 4.3 WKK305 pKa = 10.66 DD306 pKa = 3.55 DD307 pKa = 4.15 PGWFEE312 pKa = 4.81 ANVVVCEE319 pKa = 4.72 LGPNQQLWEE328 pKa = 4.28 VPNN331 pKa = 3.99
Molecular weight: 38.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.753
IPC2_protein 5.703
IPC_protein 5.753
Toseland 6.135
ProMoST 6.097
Dawson 6.02
Bjellqvist 6.02
Wikipedia 6.02
Rodwell 6.008
Grimsley 6.275
Solomon 6.02
Lehninger 6.008
Nozaki 6.287
DTASelect 6.478
Thurlkill 6.478
EMBOSS 6.44
Sillero 6.389
Patrickios 4.151
IPC_peptide 6.033
IPC2_peptide 6.389
IPC2.peptide.svr19 6.313
Protein with the highest isoelectric point:
>tr|A0A2P1E315|A0A2P1E315_9VIRU Replication associated protein OS=Termite associated circular virus 4 OX=2108552 PE=3 SV=1
MM1 pKa = 6.87 PQRR4 pKa = 11.84 RR5 pKa = 11.84 ARR7 pKa = 11.84 TRR9 pKa = 11.84 RR10 pKa = 11.84 TARR13 pKa = 11.84 KK14 pKa = 8.77 GRR16 pKa = 11.84 KK17 pKa = 7.9 STTTRR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 8.72 QPLVKK29 pKa = 10.42 VMRR32 pKa = 11.84 RR33 pKa = 11.84 VARR36 pKa = 11.84 QQINRR41 pKa = 11.84 AVEE44 pKa = 4.19 TKK46 pKa = 10.13 RR47 pKa = 11.84 FAIINEE53 pKa = 4.31 SWSSTFIDD61 pKa = 4.24 FATRR65 pKa = 11.84 NTTWKK70 pKa = 10.08 YY71 pKa = 11.28 KK72 pKa = 10.85 NLFSQLGTGNTSWSIVGNEE91 pKa = 4.17 ILNPMVKK98 pKa = 9.43 IKK100 pKa = 10.69 GRR102 pKa = 11.84 IWVDD106 pKa = 2.79 WNANRR111 pKa = 11.84 QINTVVGPQDD121 pKa = 3.52 VQVTVYY127 pKa = 10.4 LIAANEE133 pKa = 4.07 QLSTTAPSPLLASSDD148 pKa = 3.43 YY149 pKa = 10.74 WFYY152 pKa = 11.36 QPDD155 pKa = 3.99 GQNPTLNGNNVKK167 pKa = 10.42 VLGRR171 pKa = 11.84 TSKK174 pKa = 10.52 KK175 pKa = 10.06 IKK177 pKa = 10.09 AQLPIAAATTSTFSGTSTTRR197 pKa = 11.84 FQMSYY202 pKa = 7.46 RR203 pKa = 11.84 WKK205 pKa = 10.64 RR206 pKa = 11.84 KK207 pKa = 7.73 LTYY210 pKa = 9.94 EE211 pKa = 4.21 DD212 pKa = 4.09 VVGNPPSVGGPTRR225 pKa = 11.84 DD226 pKa = 3.04 VYY228 pKa = 11.34 LRR230 pKa = 11.84 GWNYY234 pKa = 9.52 YY235 pKa = 9.59 VLLGVHH241 pKa = 6.35 VPQTHH246 pKa = 6.16 NGSLVGTSTNVSMDD260 pKa = 2.59 TFMYY264 pKa = 10.82 YY265 pKa = 10.34 KK266 pKa = 10.69 DD267 pKa = 3.5 PP268 pKa = 4.35
Molecular weight: 30.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.087
IPC_protein 10.906
Toseland 10.906
ProMoST 10.672
Dawson 10.994
Bjellqvist 10.73
Wikipedia 11.242
Rodwell 11.228
Grimsley 11.052
Solomon 11.14
Lehninger 11.096
Nozaki 10.877
DTASelect 10.73
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.935
Patrickios 10.935
IPC_peptide 11.14
IPC2_peptide 9.633
IPC2.peptide.svr19 8.2
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
599
268
331
299.5
34.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.175 ± 0.273
1.503 ± 0.973
5.342 ± 1.285
5.342 ± 2.251
4.841 ± 1.202
6.344 ± 0.0
2.504 ± 1.138
3.673 ± 0.521
5.175 ± 0.515
6.511 ± 0.35
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.669 ± 0.369
4.674 ± 1.564
5.342 ± 0.077
4.841 ± 0.248
8.514 ± 0.044
7.179 ± 0.184
7.846 ± 2.167
6.344 ± 1.449
3.506 ± 0.337
3.673 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here