Listeria phage P70

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Homburgvirus; Listeria virus P70

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 119 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9QSN7|J9QSN7_9CAUD Uncharacterized protein OS=Listeria phage P70 OX=1225800 GN=P70_0080 PE=4 SV=1
MM1 pKa = 7.46NEE3 pKa = 3.73LRR5 pKa = 11.84SLEE8 pKa = 3.94MSINAKK14 pKa = 10.41DD15 pKa = 3.45YY16 pKa = 8.38ATRR19 pKa = 11.84LEE21 pKa = 4.22SGEE24 pKa = 3.77GSLYY28 pKa = 10.33IRR30 pKa = 11.84FGDD33 pKa = 3.86SEE35 pKa = 5.19DD36 pKa = 4.33YY37 pKa = 10.34PVHH40 pKa = 6.92ASTNSTIKK48 pKa = 9.48EE49 pKa = 4.18TFIEE53 pKa = 4.23LFKK56 pKa = 11.29NGWNGYY62 pKa = 9.07EE63 pKa = 4.15EE64 pKa = 5.54DD65 pKa = 3.93EE66 pKa = 4.6QEE68 pKa = 4.42LAEE71 pKa = 4.8DD72 pKa = 3.79MQEE75 pKa = 3.89IAQEE79 pKa = 4.48LILEE83 pKa = 4.24EE84 pKa = 3.95LTDD87 pKa = 3.39IFEE90 pKa = 4.68EE91 pKa = 5.04YY92 pKa = 10.43EE93 pKa = 4.05FSTDD97 pKa = 5.17EE98 pKa = 4.1IDD100 pKa = 3.28TDD102 pKa = 4.14LFSGFTFHH110 pKa = 8.41VDD112 pKa = 2.95MDD114 pKa = 4.05NDD116 pKa = 3.54EE117 pKa = 4.92AVYY120 pKa = 11.0LMDD123 pKa = 6.61AINATKK129 pKa = 10.45YY130 pKa = 10.47FEE132 pKa = 4.82ARR134 pKa = 11.84PSSWYY139 pKa = 10.52ALLEE143 pKa = 3.93VSYY146 pKa = 11.07CGG148 pKa = 4.05

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9QIW7|J9QIW7_9CAUD Replication initiator protein OS=Listeria phage P70 OX=1225800 GN=P70_0034 PE=4 SV=1
MM1 pKa = 7.37NIIYY5 pKa = 8.39MVNYY9 pKa = 9.78ASKK12 pKa = 9.74RR13 pKa = 11.84CPRR16 pKa = 11.84LVSKK20 pKa = 10.62IIARR24 pKa = 11.84SYY26 pKa = 8.28KK27 pKa = 8.68TSRR30 pKa = 11.84TSTVTNVFVLSFVPSFNLLLPVGG53 pKa = 3.97

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

119

0

119

20210

36

970

169.8

19.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.66 ± 0.336

0.925 ± 0.111

6.136 ± 0.181

7.966 ± 0.425

3.924 ± 0.158

5.972 ± 0.203

1.564 ± 0.118

6.823 ± 0.218

7.506 ± 0.368

7.788 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.147

5.73 ± 0.226

3.053 ± 0.195

3.38 ± 0.187

4.236 ± 0.177

6.329 ± 0.249

6.625 ± 0.339

7.051 ± 0.24

1.385 ± 0.089

4.28 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski