Amino acid dipepetide frequency for Prevotella sp. CAG:487

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.695AlaAla: 6.695 ± 0.128	1.137AlaCys: 1.137 ± 0.038	5.16AlaAsp: 5.16 ± 0.091	4.898AlaGlu: 4.898 ± 0.091	3.22AlaPhe: 3.22 ± 0.074	5.748AlaGly: 5.748 ± 0.109	1.349AlaHis: 1.349 ± 0.048	4.29AlaIle: 4.29 ± 0.094	3.663AlaLys: 3.663 ± 0.077	6.531AlaLeu: 6.531 ± 0.105	2.32AlaMet: 2.32 ± 0.055	2.871AlaAsn: 2.871 ± 0.066	2.353AlaPro: 2.353 ± 0.062	2.492AlaGln: 2.492 ± 0.066	4.042AlaArg: 4.042 ± 0.077	4.429AlaSer: 4.429 ± 0.071	3.963AlaThr: 3.963 ± 0.076	5.56AlaVal: 5.56 ± 0.088	0.805AlaTrp: 0.805 ± 0.033	3.006AlaTyr: 3.006 ± 0.066	0.006AlaXaa: 0.006 ± 0.003
Cys	1.036CysAla: 1.036 ± 0.039	0.237CysCys: 0.237 ± 0.019	0.874CysAsp: 0.874 ± 0.034	0.739CysGlu: 0.739 ± 0.032	0.619CysPhe: 0.619 ± 0.031	1.419CysGly: 1.419 ± 0.05	0.378CysHis: 0.378 ± 0.024	0.925CysIle: 0.925 ± 0.034	0.667CysLys: 0.667 ± 0.026	1.181CysLeu: 1.181 ± 0.039	0.466CysMet: 0.466 ± 0.025	0.619CysAsn: 0.619 ± 0.027	0.629CysPro: 0.629 ± 0.033	0.319CysGln: 0.319 ± 0.021	0.967CysArg: 0.967 ± 0.037	0.907CysSer: 0.907 ± 0.037	0.708CysThr: 0.708 ± 0.029	1.028CysVal: 1.028 ± 0.039	0.159CysTrp: 0.159 ± 0.016	0.566CysTyr: 0.566 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.512AspAla: 4.512 ± 0.072	0.815AspCys: 0.815 ± 0.037	3.708AspAsp: 3.708 ± 0.087	4.056AspGlu: 4.056 ± 0.075	2.915AspPhe: 2.915 ± 0.061	5.721AspGly: 5.721 ± 0.116	0.795AspHis: 0.795 ± 0.032	4.714AspIle: 4.714 ± 0.074	3.793AspLys: 3.793 ± 0.086	3.909AspLeu: 3.909 ± 0.071	2.072AspMet: 2.072 ± 0.051	3.569AspAsn: 3.569 ± 0.074	1.768AspPro: 1.768 ± 0.056	0.929AspGln: 0.929 ± 0.032	3.114AspArg: 3.114 ± 0.07	3.77AspSer: 3.77 ± 0.092	3.21AspThr: 3.21 ± 0.067	4.209AspVal: 4.209 ± 0.094	0.805AspTrp: 0.805 ± 0.037	2.829AspTyr: 2.829 ± 0.069	0.001AspXaa: 0.001 ± 0.001
Glu	4.333GluAla: 4.333 ± 0.072	0.76GluCys: 0.76 ± 0.034	3.194GluAsp: 3.194 ± 0.074	4.01GluGlu: 4.01 ± 0.087	2.193GluPhe: 2.193 ± 0.052	3.862GluGly: 3.862 ± 0.065	1.291GluHis: 1.291 ± 0.038	3.869GluIle: 3.869 ± 0.078	4.387GluLys: 4.387 ± 0.088	5.198GluLeu: 5.198 ± 0.092	2.015GluMet: 2.015 ± 0.056	3.302GluAsn: 3.302 ± 0.073	1.812GluPro: 1.812 ± 0.05	2.202GluGln: 2.202 ± 0.055	3.72GluArg: 3.72 ± 0.079	3.228GluSer: 3.228 ± 0.066	3.257GluThr: 3.257 ± 0.072	3.556GluVal: 3.556 ± 0.069	0.811GluTrp: 0.811 ± 0.033	2.81GluTyr: 2.81 ± 0.069	0.001GluXaa: 0.001 ± 0.001
Phe	3.051PheAla: 3.051 ± 0.068	0.75PheCys: 0.75 ± 0.031	2.841PheAsp: 2.841 ± 0.061	2.196PheGlu: 2.196 ± 0.054	2.067PhePhe: 2.067 ± 0.059	3.271PheGly: 3.271 ± 0.07	0.856PheHis: 0.856 ± 0.034	2.956PheIle: 2.956 ± 0.076	2.067PheLys: 2.067 ± 0.06	3.387PheLeu: 3.387 ± 0.083	1.324PheMet: 1.324 ± 0.046	2.168PheAsn: 2.168 ± 0.057	1.601PhePro: 1.601 ± 0.048	0.871PheGln: 0.871 ± 0.034	2.491PheArg: 2.491 ± 0.056	3.241PheSer: 3.241 ± 0.079	2.658PheThr: 2.658 ± 0.073	2.909PheVal: 2.909 ± 0.06	0.517PheTrp: 0.517 ± 0.026	1.81PheTyr: 1.81 ± 0.043	0.003PheXaa: 0.003 ± 0.002
Gly	4.705GlyAla: 4.705 ± 0.089	1.181GlyCys: 1.181 ± 0.041	4.128GlyAsp: 4.128 ± 0.079	4.191GlyGlu: 4.191 ± 0.081	3.089GlyPhe: 3.089 ± 0.063	5.354GlyGly: 5.354 ± 0.104	1.492GlyHis: 1.492 ± 0.046	5.155GlyIle: 5.155 ± 0.091	5.056GlyLys: 5.056 ± 0.087	5.703GlyLeu: 5.703 ± 0.088	2.51GlyMet: 2.51 ± 0.064	3.666GlyAsn: 3.666 ± 0.086	1.355GlyPro: 1.355 ± 0.04	2.154GlyGln: 2.154 ± 0.055	3.968GlyArg: 3.968 ± 0.085	4.417GlySer: 4.417 ± 0.08	4.528GlyThr: 4.528 ± 0.105	5.046GlyVal: 5.046 ± 0.09	1.019GlyTrp: 1.019 ± 0.042	3.366GlyTyr: 3.366 ± 0.063	0.009GlyXaa: 0.009 ± 0.004
His	1.326HisAla: 1.326 ± 0.045	0.369HisCys: 0.369 ± 0.023	1.184HisAsp: 1.184 ± 0.042	1.018HisGlu: 1.018 ± 0.035	0.959HisPhe: 0.959 ± 0.037	1.479HisGly: 1.479 ± 0.05	0.514HisHis: 0.514 ± 0.029	1.525HisIle: 1.525 ± 0.048	1.055HisLys: 1.055 ± 0.035	1.503HisLeu: 1.503 ± 0.048	0.532HisMet: 0.532 ± 0.026	0.966HisAsn: 0.966 ± 0.031	1.002HisPro: 1.002 ± 0.039	0.478HisGln: 0.478 ± 0.023	1.164HisArg: 1.164 ± 0.04	1.203HisSer: 1.203 ± 0.035	1.17HisThr: 1.17 ± 0.036	1.299HisVal: 1.299 ± 0.049	0.226HisTrp: 0.226 ± 0.017	0.891HisTyr: 0.891 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	4.893IleAla: 4.893 ± 0.09	0.995IleCys: 0.995 ± 0.039	4.351IleAsp: 4.351 ± 0.074	3.946IleGlu: 3.946 ± 0.064	2.505IlePhe: 2.505 ± 0.062	4.496IleGly: 4.496 ± 0.095	1.189IleHis: 1.189 ± 0.041	4.668IleIle: 4.668 ± 0.118	3.935IleLys: 3.935 ± 0.074	5.03IleLeu: 5.03 ± 0.09	1.923IleMet: 1.923 ± 0.063	3.366IleAsn: 3.366 ± 0.081	2.724IlePro: 2.724 ± 0.059	1.673IleGln: 1.673 ± 0.047	3.524IleArg: 3.524 ± 0.072	4.587IleSer: 4.587 ± 0.091	3.976IleThr: 3.976 ± 0.066	4.398IleVal: 4.398 ± 0.094	0.651IleTrp: 0.651 ± 0.03	2.679IleTyr: 2.679 ± 0.054	0.003IleXaa: 0.003 ± 0.002
Lys	4.693LysAla: 4.693 ± 0.079	0.586LysCys: 0.586 ± 0.028	3.862LysAsp: 3.862 ± 0.074	4.494LysGlu: 4.494 ± 0.09	1.876LysPhe: 1.876 ± 0.053	4.079LysGly: 4.079 ± 0.086	1.064LysHis: 1.064 ± 0.042	3.817LysIle: 3.817 ± 0.075	4.329LysLys: 4.329 ± 0.091	4.287LysLeu: 4.287 ± 0.084	1.962LysMet: 1.962 ± 0.048	3.144LysAsn: 3.144 ± 0.061	2.11LysPro: 2.11 ± 0.049	1.785LysGln: 1.785 ± 0.056	3.077LysArg: 3.077 ± 0.068	3.354LysSer: 3.354 ± 0.076	3.615LysThr: 3.615 ± 0.076	3.812LysVal: 3.812 ± 0.07	0.768LysTrp: 0.768 ± 0.035	2.792LysTyr: 2.792 ± 0.06	0.006LysXaa: 0.006 ± 0.002
Leu	6.085LeuAla: 6.085 ± 0.106	1.458LeuCys: 1.458 ± 0.048	4.672LeuAsp: 4.672 ± 0.086	4.065LeuGlu: 4.065 ± 0.072	3.913LeuPhe: 3.913 ± 0.076	5.254LeuGly: 5.254 ± 0.087	1.777LeuHis: 1.777 ± 0.056	4.74LeuIle: 4.74 ± 0.088	5.156LeuLys: 5.156 ± 0.088	7.327LeuLeu: 7.327 ± 0.123	2.709LeuMet: 2.709 ± 0.06	3.999LeuAsn: 3.999 ± 0.084	3.708LeuPro: 3.708 ± 0.069	2.562LeuGln: 2.562 ± 0.069	4.976LeuArg: 4.976 ± 0.103	6.339LeuSer: 6.339 ± 0.098	5.203LeuThr: 5.203 ± 0.087	4.884LeuVal: 4.884 ± 0.092	0.922LeuTrp: 0.922 ± 0.041	3.385LeuTyr: 3.385 ± 0.068	0.003LeuXaa: 0.003 ± 0.002
Met	2.618MetAla: 2.618 ± 0.058	0.413MetCys: 0.413 ± 0.024	1.595MetAsp: 1.595 ± 0.05	1.814MetGlu: 1.814 ± 0.048	1.304MetPhe: 1.304 ± 0.046	1.888MetGly: 1.888 ± 0.054	0.593MetHis: 0.593 ± 0.026	1.715MetIle: 1.715 ± 0.061	2.409MetLys: 2.409 ± 0.061	2.943MetLeu: 2.943 ± 0.069	1.055MetMet: 1.055 ± 0.037	1.588MetAsn: 1.588 ± 0.042	1.541MetPro: 1.541 ± 0.048	1.082MetGln: 1.082 ± 0.034	2.043MetArg: 2.043 ± 0.044	2.192MetSer: 2.192 ± 0.06	1.989MetThr: 1.989 ± 0.048	1.722MetVal: 1.722 ± 0.045	0.319MetTrp: 0.319 ± 0.021	1.016MetTyr: 1.016 ± 0.037	0.003MetXaa: 0.003 ± 0.002
Asn	3.769AsnAla: 3.769 ± 0.072	0.56AsnCys: 0.56 ± 0.03	2.908AsnAsp: 2.908 ± 0.066	2.788AsnGlu: 2.788 ± 0.069	1.789AsnPhe: 1.789 ± 0.051	4.05AsnGly: 4.05 ± 0.087	0.87AsnHis: 0.87 ± 0.034	3.902AsnIle: 3.902 ± 0.076	2.732AsnLys: 2.732 ± 0.065	3.556AsnLeu: 3.556 ± 0.081	1.462AsnMet: 1.462 ± 0.039	2.502AsnAsn: 2.502 ± 0.073	2.147AsnPro: 2.147 ± 0.053	1.165AsnGln: 1.165 ± 0.04	2.64AsnArg: 2.64 ± 0.064	2.787AsnSer: 2.787 ± 0.061	2.91AsnThr: 2.91 ± 0.069	3.491AsnVal: 3.491 ± 0.072	0.546AsnTrp: 0.546 ± 0.025	2.022AsnTyr: 2.022 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.826ProAla: 2.826 ± 0.067	0.438ProCys: 0.438 ± 0.025	2.838ProAsp: 2.838 ± 0.058	3.233ProGlu: 3.233 ± 0.058	1.768ProPhe: 1.768 ± 0.046	2.524ProGly: 2.524 ± 0.058	0.811ProHis: 0.811 ± 0.029	1.842ProIle: 1.842 ± 0.056	1.809ProLys: 1.809 ± 0.055	3.151ProLeu: 3.151 ± 0.061	0.989ProMet: 0.989 ± 0.033	1.284ProAsn: 1.284 ± 0.04	0.857ProPro: 0.857 ± 0.033	1.406ProGln: 1.406 ± 0.038	1.369ProArg: 1.369 ± 0.042	2.3ProSer: 2.3 ± 0.056	1.742ProThr: 1.742 ± 0.052	3.084ProVal: 3.084 ± 0.066	0.49ProTrp: 0.49 ± 0.025	1.73ProTyr: 1.73 ± 0.056	0.005ProXaa: 0.005 ± 0.003
Gln	2.044GlnAla: 2.044 ± 0.055	0.353GlnCys: 0.353 ± 0.022	1.452GlnAsp: 1.452 ± 0.043	1.562GlnGlu: 1.562 ± 0.052	1.139GlnPhe: 1.139 ± 0.035	1.698GlnGly: 1.698 ± 0.048	0.564GlnHis: 0.564 ± 0.026	2.008GlnIle: 2.008 ± 0.047	1.921GlnLys: 1.921 ± 0.053	2.63GlnLeu: 2.63 ± 0.065	1.114GlnMet: 1.114 ± 0.038	1.56GlnAsn: 1.56 ± 0.044	1.179GlnPro: 1.179 ± 0.039	1.216GlnGln: 1.216 ± 0.048	1.712GlnArg: 1.712 ± 0.055	1.766GlnSer: 1.766 ± 0.045	1.939GlnThr: 1.939 ± 0.053	1.687GlnVal: 1.687 ± 0.049	0.403GlnTrp: 0.403 ± 0.023	1.29GlnTyr: 1.29 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	3.333ArgAla: 3.333 ± 0.073	0.677ArgCys: 0.677 ± 0.03	2.852ArgAsp: 2.852 ± 0.067	3.131ArgGlu: 3.131 ± 0.073	2.462ArgPhe: 2.462 ± 0.058	3.046ArgGly: 3.046 ± 0.08	1.56ArgHis: 1.56 ± 0.042	3.932ArgIle: 3.932 ± 0.059	3.921ArgLys: 3.921 ± 0.067	5.636ArgLeu: 5.636 ± 0.102	2.24ArgMet: 2.24 ± 0.045	2.915ArgAsn: 2.915 ± 0.061	1.886ArgPro: 1.886 ± 0.05	2.41ArgGln: 2.41 ± 0.061	3.801ArgArg: 3.801 ± 0.087	2.864ArgSer: 2.864 ± 0.058	2.966ArgThr: 2.966 ± 0.063	3.065ArgVal: 3.065 ± 0.067	0.694ArgTrp: 0.694 ± 0.033	2.511ArgTyr: 2.511 ± 0.058	0.005ArgXaa: 0.005 ± 0.003
Ser	4.725SerAla: 4.725 ± 0.074	0.99SerCys: 0.99 ± 0.04	4.01SerAsp: 4.01 ± 0.072	3.486SerGlu: 3.486 ± 0.07	3.065SerPhe: 3.065 ± 0.069	5.211SerGly: 5.211 ± 0.097	1.405SerHis: 1.405 ± 0.048	3.955SerIle: 3.955 ± 0.079	3.051SerLys: 3.051 ± 0.054	5.878SerLeu: 5.878 ± 0.105	1.805SerMet: 1.805 ± 0.049	2.483SerAsn: 2.483 ± 0.066	2.459SerPro: 2.459 ± 0.061	1.773SerGln: 1.773 ± 0.053	3.437SerArg: 3.437 ± 0.076	4.051SerSer: 4.051 ± 0.096	3.225SerThr: 3.225 ± 0.061	4.985SerVal: 4.985 ± 0.09	0.809SerTrp: 0.809 ± 0.041	2.797SerTyr: 2.797 ± 0.075	0.0SerXaa: 0.0 ± 0.0
Thr	4.855ThrAla: 4.855 ± 0.081	0.671ThrCys: 0.671 ± 0.036	4.043ThrAsp: 4.043 ± 0.086	3.177ThrGlu: 3.177 ± 0.062	2.677ThrPhe: 2.677 ± 0.059	4.79ThrGly: 4.79 ± 0.078	1.072ThrHis: 1.072 ± 0.036	3.598ThrIle: 3.598 ± 0.074	2.468ThrLys: 2.468 ± 0.051	5.301ThrLeu: 5.301 ± 0.091	1.432ThrMet: 1.432 ± 0.046	2.18ThrAsn: 2.18 ± 0.053	2.744ThrPro: 2.744 ± 0.066	1.563ThrGln: 1.563 ± 0.046	2.599ThrArg: 2.599 ± 0.055	3.504ThrSer: 3.504 ± 0.076	3.21ThrThr: 3.21 ± 0.075	4.811ThrVal: 4.811 ± 0.093	0.644ThrTrp: 0.644 ± 0.034	2.466ThrTyr: 2.466 ± 0.058	0.003ThrXaa: 0.003 ± 0.002
Val	5.09ValAla: 5.09 ± 0.108	1.254ValCys: 1.254 ± 0.044	3.765ValAsp: 3.765 ± 0.072	4.032ValGlu: 4.032 ± 0.077	3.025ValPhe: 3.025 ± 0.071	4.186ValGly: 4.186 ± 0.081	1.105ValHis: 1.105 ± 0.04	4.298ValIle: 4.298 ± 0.091	4.011ValLys: 4.011 ± 0.076	5.6ValLeu: 5.6 ± 0.104	2.215ValMet: 2.215 ± 0.06	3.261ValAsn: 3.261 ± 0.059	2.667ValPro: 2.667 ± 0.053	1.569ValGln: 1.569 ± 0.045	4.087ValArg: 4.087 ± 0.078	5.077ValSer: 5.077 ± 0.091	4.261ValThr: 4.261 ± 0.078	4.819ValVal: 4.819 ± 0.099	0.773ValTrp: 0.773 ± 0.028	2.808ValTyr: 2.808 ± 0.064	0.01ValXaa: 0.01 ± 0.004
Trp	0.746TrpAla: 0.746 ± 0.031	0.176TrpCys: 0.176 ± 0.017	0.723TrpAsp: 0.723 ± 0.03	0.603TrpGlu: 0.603 ± 0.029	0.528TrpPhe: 0.528 ± 0.024	0.813TrpGly: 0.813 ± 0.029	0.32TrpHis: 0.32 ± 0.02	0.743TrpIle: 0.743 ± 0.032	0.748TrpLys: 0.748 ± 0.028	1.183TrpLeu: 1.183 ± 0.042	0.45TrpMet: 0.45 ± 0.026	0.75TrpAsn: 0.75 ± 0.031	0.277TrpPro: 0.277 ± 0.021	0.514TrpGln: 0.514 ± 0.029	0.683TrpArg: 0.683 ± 0.033	0.772TrpSer: 0.772 ± 0.036	0.755TrpThr: 0.755 ± 0.033	0.603TrpVal: 0.603 ± 0.029	0.193TrpTrp: 0.193 ± 0.017	0.475TrpTyr: 0.475 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.201TyrAla: 3.201 ± 0.068	0.618TyrCys: 0.618 ± 0.028	3.056TyrAsp: 3.056 ± 0.065	2.372TyrGlu: 2.372 ± 0.064	1.92TyrPhe: 1.92 ± 0.056	3.195TyrGly: 3.195 ± 0.077	0.854TyrHis: 0.854 ± 0.032	2.858TyrIle: 2.858 ± 0.065	2.332TyrLys: 2.332 ± 0.059	3.197TyrLeu: 3.197 ± 0.071	1.248TyrMet: 1.248 ± 0.046	2.4TyrAsn: 2.4 ± 0.062	1.674TyrPro: 1.674 ± 0.047	1.076TyrGln: 1.076 ± 0.041	2.479TyrArg: 2.479 ± 0.061	2.806TyrSer: 2.806 ± 0.073	2.521TyrThr: 2.521 ± 0.065	2.943TyrVal: 2.943 ± 0.059	0.499TyrTrp: 0.499 ± 0.024	2.008TyrTyr: 2.008 ± 0.053	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.003XaaAla: 0.003 ± 0.002	0.004XaaCys: 0.004 ± 0.002	0.001XaaAsp: 0.001 ± 0.002	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.009XaaGly: 0.009 ± 0.003	0.003XaaHis: 0.003 ± 0.002	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.002	0.004XaaLeu: 0.004 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.003XaaAsn: 0.003 ± 0.002	0.004XaaPro: 0.004 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.015XaaArg: 0.015 ± 0.004	0.006XaaSer: 0.006 ± 0.003	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.066XaaXaa: 0.066 ± 0.012

Statistics based on 2507 proteins (783767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---