Methanobrevibacter cuticularis

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166CGS6|A0A166CGS6_9EURY Bacterial Ig-like domain protein OS=Methanobrevibacter cuticularis OX=47311 GN=MBCUT_20640 PE=3 SV=1
MM1 pKa = 7.27NKK3 pKa = 9.78KK4 pKa = 9.45NQLEE8 pKa = 4.03FDD10 pKa = 4.02YY11 pKa = 10.92NLQIGVDD18 pKa = 3.87DD19 pKa = 3.84YY20 pKa = 11.89KK21 pKa = 11.5GIVLSIGVSNNPTDD35 pKa = 4.01FNEE38 pKa = 5.43LIPQIIQIEE47 pKa = 4.35EE48 pKa = 4.17NIGDD52 pKa = 4.02LTEE55 pKa = 4.14NTQISADD62 pKa = 3.52NGYY65 pKa = 8.22STDD68 pKa = 3.24EE69 pKa = 3.77NMEE72 pKa = 3.93YY73 pKa = 10.77LEE75 pKa = 3.89EE76 pKa = 4.56HH77 pKa = 6.76RR78 pKa = 11.84LDD80 pKa = 3.88SYY82 pKa = 11.43II83 pKa = 4.18

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166FL71|A0A166FL71_9EURY NADH dehydrogenase OS=Methanobrevibacter cuticularis OX=47311 GN=nox PE=4 SV=1
MM1 pKa = 7.76LPRR4 pKa = 11.84KK5 pKa = 9.15YY6 pKa = 10.7GKK8 pKa = 9.87AARR11 pKa = 11.84KK12 pKa = 9.25CSRR15 pKa = 11.84CGDD18 pKa = 3.56HH19 pKa = 6.6SAMIRR24 pKa = 11.84RR25 pKa = 11.84YY26 pKa = 10.5GLMLCRR32 pKa = 11.84QCFRR36 pKa = 11.84EE37 pKa = 4.12VAPKK41 pKa = 10.25IGFKK45 pKa = 10.41KK46 pKa = 10.7YY47 pKa = 9.54NN48 pKa = 3.3

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2061

0

2061

590290

29

3850

286.4

32.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.418 ± 0.061

1.037 ± 0.029

5.829 ± 0.051

6.844 ± 0.094

4.223 ± 0.047

6.517 ± 0.078

1.505 ± 0.025

10.315 ± 0.063

8.688 ± 0.1

8.517 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.186 ± 0.029

7.954 ± 0.159

3.046 ± 0.044

2.047 ± 0.029

2.96 ± 0.043

6.931 ± 0.065

5.633 ± 0.1

6.044 ± 0.062

0.617 ± 0.018

3.689 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski