Halopiger salifodinae

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halopiger

Average proteome isoelectric point is 4.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4090 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0NPH5|A0A1I0NPH5_9EURY Kynurenine formamidase OS=Halopiger salifodinae OX=1202768 GN=SAMN05216285_1923 PE=4 SV=1
MM1 pKa = 7.7ANDD4 pKa = 4.68DD5 pKa = 4.12NNTSKK10 pKa = 9.2TCRR13 pKa = 11.84IGKK16 pKa = 7.86TSVGRR21 pKa = 11.84RR22 pKa = 11.84TFLGAAGAGAVATTLAGCLGGGDD45 pKa = 4.52DD46 pKa = 5.23DD47 pKa = 5.43GFTIGHH53 pKa = 7.6LAPLEE58 pKa = 4.02NTQGLGSEE66 pKa = 4.47QSAEE70 pKa = 3.95LAAKK74 pKa = 9.33EE75 pKa = 4.15INDD78 pKa = 3.93DD79 pKa = 3.6GGIRR83 pKa = 11.84DD84 pKa = 3.93EE85 pKa = 4.41DD86 pKa = 4.12VEE88 pKa = 4.43IVSADD93 pKa = 3.48TRR95 pKa = 11.84SDD97 pKa = 3.2PSTAQDD103 pKa = 3.29EE104 pKa = 4.46ASRR107 pKa = 11.84LINQEE112 pKa = 3.82RR113 pKa = 11.84VDD115 pKa = 4.12LLVGTFSSEE124 pKa = 3.85VSVNIMDD131 pKa = 5.01MIAEE135 pKa = 4.07NDD137 pKa = 3.17VPYY140 pKa = 10.31IVTGSASPAIIEE152 pKa = 4.23NSTGSDD158 pKa = 3.43YY159 pKa = 11.23EE160 pKa = 4.07ANKK163 pKa = 10.68NIFRR167 pKa = 11.84SGPINSYY174 pKa = 9.95FQAEE178 pKa = 4.32AMGGYY183 pKa = 10.43ADD185 pKa = 4.54YY186 pKa = 11.26LSDD189 pKa = 3.27HH190 pKa = 6.77HH191 pKa = 6.6GWNTFAYY198 pKa = 10.14LADD201 pKa = 4.28DD202 pKa = 4.85AAWTDD207 pKa = 3.6PFTNNLPDD215 pKa = 3.61EE216 pKa = 4.69LEE218 pKa = 4.0SRR220 pKa = 11.84GYY222 pKa = 10.68DD223 pKa = 3.14VVYY226 pKa = 10.38EE227 pKa = 4.47SEE229 pKa = 4.57LSTGIDD235 pKa = 3.4DD236 pKa = 5.2FSTVMSDD243 pKa = 4.64LEE245 pKa = 4.42SEE247 pKa = 4.32EE248 pKa = 4.01PDD250 pKa = 3.13AVFRR254 pKa = 11.84FFAHH258 pKa = 7.54IIATDD263 pKa = 3.79MLASWHH269 pKa = 5.18QRR271 pKa = 11.84QAGFGIEE278 pKa = 4.55GIHH281 pKa = 5.67VASMTPDD288 pKa = 4.31FYY290 pKa = 11.42QLSEE294 pKa = 4.04GTATFEE300 pKa = 4.36TTSQSGAAGTTAITDD315 pKa = 3.51KK316 pKa = 10.95TLDD319 pKa = 3.79FVDD322 pKa = 5.08EE323 pKa = 4.32YY324 pKa = 11.59QAFTEE329 pKa = 4.96DD330 pKa = 5.03DD331 pKa = 4.32DD332 pKa = 4.71GAPDD336 pKa = 3.57LPMYY340 pKa = 10.22MGFNTYY346 pKa = 10.57DD347 pKa = 3.54AIYY350 pKa = 9.68LYY352 pKa = 10.79RR353 pKa = 11.84EE354 pKa = 4.17AVEE357 pKa = 4.22EE358 pKa = 4.54AGTADD363 pKa = 3.89YY364 pKa = 11.1EE365 pKa = 4.53SDD367 pKa = 4.13LDD369 pKa = 4.95DD370 pKa = 5.1IVDD373 pKa = 3.57AMLGLDD379 pKa = 3.6YY380 pKa = 10.83TGTAGEE386 pKa = 3.79ISFYY390 pKa = 11.37GEE392 pKa = 3.67GDD394 pKa = 3.83EE395 pKa = 4.88YY396 pKa = 10.8PHH398 pKa = 7.25DD399 pKa = 3.95VQEE402 pKa = 4.36EE403 pKa = 4.18RR404 pKa = 11.84GEE406 pKa = 4.11GDD408 pKa = 4.22AITNFPMTQWRR419 pKa = 11.84PEE421 pKa = 3.82GGLEE425 pKa = 4.07CVYY428 pKa = 10.6PEE430 pKa = 3.83QHH432 pKa = 5.63RR433 pKa = 11.84TADD436 pKa = 3.41HH437 pKa = 6.09VQPEE441 pKa = 4.38WMSS444 pKa = 3.25

Molecular weight: 48.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0MMJ3|A0A1I0MMJ3_9EURY Uncharacterized protein OS=Halopiger salifodinae OX=1202768 GN=SAMN05216285_1118 PE=4 SV=1
MM1 pKa = 8.29RR2 pKa = 11.84YY3 pKa = 9.14RR4 pKa = 11.84EE5 pKa = 3.64RR6 pKa = 11.84SIRR9 pKa = 11.84RR10 pKa = 11.84GQRR13 pKa = 11.84RR14 pKa = 11.84QHH16 pKa = 3.58VHH18 pKa = 5.57KK19 pKa = 10.57RR20 pKa = 11.84LAEE23 pKa = 3.98LEE25 pKa = 4.27RR26 pKa = 11.84EE27 pKa = 4.25LEE29 pKa = 4.11EE30 pKa = 3.69TRR32 pKa = 11.84DD33 pKa = 3.32RR34 pKa = 11.84VRR36 pKa = 11.84QLEE39 pKa = 4.03NTLRR43 pKa = 11.84GVVQNANDD51 pKa = 3.48VSIGGPCKK59 pKa = 10.2CGKK62 pKa = 9.91SLLLIRR68 pKa = 11.84QQKK71 pKa = 9.4IYY73 pKa = 10.48CPHH76 pKa = 6.73CGYY79 pKa = 10.58NRR81 pKa = 11.84TMM83 pKa = 4.06

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4090

0

4090

1200987

29

1673

293.6

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.047 ± 0.051

0.754 ± 0.014

8.856 ± 0.047

8.89 ± 0.055

3.219 ± 0.025

8.478 ± 0.034

1.938 ± 0.019

4.333 ± 0.031

1.69 ± 0.02

8.744 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.712 ± 0.016

2.314 ± 0.021

4.741 ± 0.025

2.399 ± 0.027

6.647 ± 0.046

5.399 ± 0.029

6.336 ± 0.029

8.639 ± 0.038

1.143 ± 0.016

2.72 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski